GCA_000286295.1 - 6 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

ALIF01000007.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 1.1	lanthipeptide-class-ii	3	20,469	streptococcin A-FF22	RiPP:Lanthipeptide	50%
Region 1.2	NRPS,transAT-PKS	23,735	78,782	thermophilin 1277	RiPP:Lanthipeptide	10%
Region 1.3	lanthipeptide-class-ii	164,555	185,045	salivaricin A	RiPP:Lanthipeptide	87%

ALIF01000001.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 2.1	RiPP-like	280,309	292,405
Region 2.2	T3PKS	568,534	609,700	coumermycin A1	Saccharide:Hybrid/tailoring + Other:Aminocoumarin	6%

ALIF01000006.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 7.1	RiPP-like	296,267	315,058

Compact view

ALIF01000007.1 - Region 1 - lanthipeptide-class-ii

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Location: 3 - 20,469 nt. (total: 20,467 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

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core biosynthetic genes

additional biosynthetic genes

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Gene details

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Lanthipeptides

KnownClusterBlast

Lanthipeptide predictions

Lanthipeptide(s) for ctg1_10 - Putative Class II

ctg1_9 leader / core peptide: MAKQQMNLVEIEAMNSLQELTLEELDNVLGA - GGGVIQDhbIDhaHECRMNDhaWQFLFDhbCCDha
allorf_001966_002037 leader / core peptide: MYCRKRQLHWG - CRFYLVFAIIGDha

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Similar known gene clusters

Lanthipeptides

Core peptide predictions

ctg1_9 - Class II

Cleavage pHMM score: 5.20

RODEO score: 0

Monoisotopic mass: 2713.2 Da

Molecular weight: 2715.1 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 2733.1 Da N = 1; 2751.1 Da N = 2

allorf_001966_002037 - Class II

Cleavage pHMM score: -6.30

RODEO score: 0

Monoisotopic mass: 1369.7 Da

Molecular weight: 1370.7 Da

Number of bridges: 1

ALIF01000007.1 - Region 2 - NRPS,transAT-PKS

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Location: 23,735 - 78,782 nt. (total: 55,048 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))
transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))

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core biosynthetic genes

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Gene details

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 2, location 23734 - 78782:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - mal) + (cys) + (ohmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 23734 - 69305:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - mal) + (cys) + (ohmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 31631 - 78782:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - mal) + (cys) + (ohmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg1_43

Search NORINE for peptide: strict or relaxed

AMP-binding (11..360): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: phe, trp
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: ipWTIldtlK
Nearest Stachelhaus code:: phe
Stachelhaus code match:: 40% (weak)

ctg1_44

Search NORINE for peptide: strict or relaxed

AMP-binding (824..1226): cys

NRPSPredictor2: cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: cys
Small clusters prediction:: cys
Single AA prediction:: cys

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNlSLVWK
Nearest Stachelhaus code:: cys
Stachelhaus code match:: 90% (moderate)

ctg1_49

PKS_AT (6..282): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 70.8%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 67.0
inactive: 35.5
Methoxymalonyl-CoA: 15.9
Methylmalonyl-CoA: 11.2
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ALIF01000007.1 - Region 3 - lanthipeptide-class-ii

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Location: 164,555 - 185,045 nt. (total: 20,491 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Lanthipeptides

KnownClusterBlast

Lanthipeptide predictions

Lanthipeptide(s) for ctg1_167 - Putative Class II

ctg1_166 leader / core peptide: MKNSKDILNNAIEEVSEKELMEVAGG - KRGDhbGWFADhbIDhbDDCPNDhaVFVCC

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Similar known gene clusters

Lanthipeptides

Core peptide predictions

ctg1_166 - Class II

Cleavage pHMM score: 4.40

RODEO score: 0

Monoisotopic mass: 2347.1 Da

Molecular weight: 2348.7 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 2366.7 Da N = 1

ALIF01000001.1 - Region 1 - RiPP-like

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Location: 280,309 - 292,405 nt. (total: 12,097 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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KnownClusterBlast

Similar known gene clusters

No matches found.

ALIF01000001.1 - Region 2 - T3PKS

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Location: 568,534 - 609,700 nt. (total: 41,167 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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KnownClusterBlast

Similar known gene clusters

ALIF01000006.1 - Region 1 - RiPP-like

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Location: 296,267 - 315,058 nt. (total: 18,792 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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KnownClusterBlast

Similar known gene clusters

No matches found.