GCA_000409585.1 - 20 region(s)

Identified secondary metabolite regions using strictness 'relaxed'

AMCA01000008.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 6.1	NRPS	1	5,333	mycosubtilin	NRP + Polyketide	30%

AMCA01000011.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 9.1	NRPS	1	10,220	surfactin	NRP:Lipopeptide	8%

AMCA01000030.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 28.1	NRPS	1	7,817	plipastatin	NRP	23%

AMCA01000031.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 29.1	betalactone	1	13,500	fengycin	NRP	60%

AMCA01000075.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 72.1	transAT-PKS,T3PKS,PKS-like,NRPS	1	100,580	bacillaene	Polyketide + NRP	100%

AMCA01000083.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 80.1	NRPS	1	2,241

AMCA01000088.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 83.1	NRPS	1	5,605	surfactin	NRP:Lipopeptide	17%

AMCA01000100.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 94.1	NRPS	1	10,135	surfactin	NRP:Lipopeptide	17%

AMCA01000118.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 110.1	T3PKS	1	25,184

AMCA01000124.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 116.1	NRPS	1	12,553	bacillibactin	NRP	53%

AMCA01000190.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 172.1	terpene	1	14,145

AMCA01000222.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 197.1	lanthipeptide-class-i	17,646	31,388	subtilin	RiPP:Lanthipeptide	100%

AMCA01000223.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 198.1	RRE-containing	1	12,582

AMCA01000296.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 262.1	other	1	7,390	bacilysin	Other	100%

AMCA01000301.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 266.1	sactipeptide	1	11,537	subtilosin A	RiPP:Thiopeptide	25%

AMCA01000316.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 281.1	CDPS	5,640	26,395

AMCA01000327.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 284.1	NRPS	1	13,606	fengycin	NRP	26%

AMCA01000337.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 290.1	terpene	1	7,293

AMCA01000340.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 293.1	NRPS	1	7,061	plipastatin	NRP	30%

AMCA01000343.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 295.1	NRPS	1	9,994	plipastatin	NRP	23%

No secondary metabolite regions were found in these records:: AMCA01000001.1; AMCA01000002.1; AMCA01000003.1; AMCA01000005.1; AMCA01000007.1; AMCA01000009.1; AMCA01000010.1; AMCA01000012.1; AMCA01000013.1; AMCA01000014.1; AMCA01000015.1; AMCA01000016.1; AMCA01000017.1; AMCA01000018.1; AMCA01000019.1; AMCA01000020.1; AMCA01000021.1; AMCA01000022.1; AMCA01000023.1; AMCA01000024.1; AMCA01000025.1; AMCA01000026.1; AMCA01000027.1; AMCA01000028.1; AMCA01000029.1; AMCA01000032.1; AMCA01000033.1; AMCA01000034.1; AMCA01000035.1; AMCA01000036.1; AMCA01000037.1; AMCA01000038.1; AMCA01000039.1; AMCA01000040.1; AMCA01000042.1; AMCA01000043.1; AMCA01000044.1; AMCA01000045.1; AMCA01000046.1; AMCA01000047.1; AMCA01000048.1; AMCA01000049.1; AMCA01000050.1; AMCA01000051.1; AMCA01000052.1; AMCA01000053.1; AMCA01000054.1; AMCA01000055.1; AMCA01000056.1; AMCA01000057.1; AMCA01000058.1; AMCA01000059.1; AMCA01000060.1; AMCA01000061.1; AMCA01000062.1; AMCA01000063.1; AMCA01000064.1; AMCA01000065.1; AMCA01000066.1; AMCA01000067.1; AMCA01000068.1; AMCA01000069.1; AMCA01000070.1; AMCA01000071.1; AMCA01000072.1; AMCA01000073.1; AMCA01000074.1; AMCA01000076.1; AMCA01000077.1; AMCA01000078.1; AMCA01000079.1; AMCA01000080.1; AMCA01000081.1; AMCA01000082.1; AMCA01000084.1; AMCA01000086.1; AMCA01000089.1; AMCA01000091.1; AMCA01000092.1; AMCA01000093.1; AMCA01000094.1; AMCA01000095.1; AMCA01000096.1; AMCA01000097.1; AMCA01000098.1; AMCA01000099.1; AMCA01000101.1; AMCA01000102.1; AMCA01000103.1; AMCA01000104.1; AMCA01000105.1; AMCA01000106.1; AMCA01000107.1; AMCA01000108.1; AMCA01000109.1; AMCA01000111.1; AMCA01000112.1; AMCA01000113.1; AMCA01000114.1; AMCA01000115.1; AMCA01000116.1; AMCA01000119.1; AMCA01000120.1; AMCA01000121.1; AMCA01000122.1; AMCA01000123.1; AMCA01000125.1; AMCA01000126.1; AMCA01000127.1; AMCA01000128.1; AMCA01000129.1; AMCA01000130.1; AMCA01000131.1; AMCA01000132.1; AMCA01000133.1; AMCA01000134.1; AMCA01000135.1; AMCA01000138.1; AMCA01000139.1; AMCA01000140.1; AMCA01000141.1; AMCA01000142.1; AMCA01000143.1; AMCA01000144.1; AMCA01000145.1; AMCA01000146.1; AMCA01000147.1; AMCA01000148.1; AMCA01000149.1; AMCA01000150.1; AMCA01000151.1; AMCA01000152.1; AMCA01000153.1; AMCA01000154.1; AMCA01000155.1; AMCA01000156.1; AMCA01000157.1; AMCA01000159.1; AMCA01000160.1; AMCA01000161.1; AMCA01000162.1; AMCA01000163.1; AMCA01000166.1; AMCA01000167.1; AMCA01000168.1; AMCA01000169.1; AMCA01000170.1; AMCA01000171.1; AMCA01000172.1; AMCA01000174.1; AMCA01000175.1; AMCA01000176.1; AMCA01000177.1; AMCA01000178.1; AMCA01000183.1; AMCA01000184.1; AMCA01000185.1; AMCA01000186.1; AMCA01000187.1; AMCA01000188.1; AMCA01000189.1; AMCA01000191.1; AMCA01000192.1; AMCA01000194.1; AMCA01000195.1; AMCA01000196.1; AMCA01000197.1; AMCA01000198.1; AMCA01000200.1; AMCA01000201.1; AMCA01000202.1; AMCA01000203.1; AMCA01000204.1; AMCA01000205.1; AMCA01000206.1; AMCA01000207.1; AMCA01000208.1; AMCA01000209.1; AMCA01000215.1; AMCA01000216.1; AMCA01000217.1; AMCA01000218.1; AMCA01000219.1; AMCA01000220.1; AMCA01000221.1; AMCA01000224.1; AMCA01000225.1; AMCA01000226.1; AMCA01000227.1; AMCA01000228.1; AMCA01000229.1; AMCA01000230.1; AMCA01000231.1; AMCA01000232.1; AMCA01000233.1; AMCA01000234.1; AMCA01000235.1; AMCA01000236.1; AMCA01000237.1; AMCA01000238.1; AMCA01000240.1; AMCA01000241.1; AMCA01000242.1; AMCA01000243.1; AMCA01000244.1; AMCA01000245.1; AMCA01000246.1; AMCA01000247.1; AMCA01000248.1; AMCA01000249.1; AMCA01000250.1; AMCA01000251.1; AMCA01000252.1; AMCA01000253.1; AMCA01000254.1; AMCA01000255.1; AMCA01000256.1; AMCA01000257.1; AMCA01000258.1; AMCA01000259.1; AMCA01000260.1; AMCA01000261.1; AMCA01000262.1; AMCA01000263.1; AMCA01000264.1; AMCA01000265.1; AMCA01000266.1; AMCA01000267.1; AMCA01000268.1; AMCA01000269.1; AMCA01000270.1; AMCA01000271.1; AMCA01000272.1; AMCA01000273.1; AMCA01000274.1; AMCA01000275.1; AMCA01000276.1; AMCA01000278.1; AMCA01000279.1; AMCA01000280.1; AMCA01000281.1; AMCA01000282.1; AMCA01000283.1; AMCA01000287.1; AMCA01000288.1; AMCA01000289.1; AMCA01000291.1; AMCA01000295.1; AMCA01000298.1; AMCA01000299.1; AMCA01000300.1; AMCA01000302.1; AMCA01000303.1; AMCA01000304.1; AMCA01000305.1; AMCA01000306.1; AMCA01000307.1; AMCA01000308.1; AMCA01000309.1; AMCA01000310.1; AMCA01000311.1; AMCA01000312.1; AMCA01000313.1; AMCA01000314.1; AMCA01000315.1; AMCA01000317.1; AMCA01000318.1; AMCA01000328.1; AMCA01000329.1; AMCA01000330.1; AMCA01000331.1; AMCA01000336.1; AMCA01000338.1; AMCA01000339.1; AMCA01000341.1

Compact view

AMCA01000008.1 - Region 1 - NRPS

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Location: 1 - 5,333 nt. (total: 5,333 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 5333:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (val)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg6_1

Search NORINE for peptide: strict or relaxed

AMP-binding (265..663): X

NRPSPredictor2: hydrophobic-aliphatic

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVtnFaiIyK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 50% (weak)

ctg6_2

Search NORINE for peptide: strict or relaxed

AMP-binding (0..364): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: val

Stachelhaus prediction details:

Stachelhaus sequence:: DAFWIGGTFK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 100% (strong)

AMCA01000011.1 - Region 1 - NRPS

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Location: 1 - 10,220 nt. (total: 10,220 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 10220:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (val - asp)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg9_1

Search NORINE for peptide: strict or relaxed

AMP-binding (470..864): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: val

Stachelhaus prediction details:

Stachelhaus sequence:: DAFWIGGTFK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 100% (strong)

AMP-binding (1501..1904): asp

NRPSPredictor2: asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asp

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKVGHIGK
Nearest Stachelhaus code:: asp
Stachelhaus code match:: 100% (strong)

AMP-binding (2546..2883): leu

NRPSPredictor2: leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: leu

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFLGNVVK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 100% (strong)

AMCA01000030.1 - Region 1 - NRPS

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Location: 1 - 7,817 nt. (total: 7,817 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 7817:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-tyr) + (ile)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg28_1

Search NORINE for peptide: strict or relaxed

AMP-binding (248..642): tyr

NRPSPredictor2: tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: phe, trp, phg, tyr, bht
Small clusters prediction:: tyr, bht
Single AA prediction:: tyr

Stachelhaus prediction details:

Stachelhaus sequence:: DGTITAEVAK
Nearest Stachelhaus code:: tyr
Stachelhaus code match:: 100% (strong)

ctg28_2

Search NORINE for peptide: strict or relaxed

AMP-binding (474..867): ile

NRPSPredictor2: ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: ile

Stachelhaus prediction details:

Stachelhaus sequence:: DAFFYGITFK
Nearest Stachelhaus code:: ile
Stachelhaus code match:: 100% (strong)

AMCA01000031.1 - Region 1 - betalactone

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Location: 1 - 13,500 nt. (total: 13,500 nt) Show pHMM detection rules used

Region on contig edge.

betalactone: (HMGL-like and AMP-binding)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

KnownClusterBlast

Similar known gene clusters

AMCA01000075.1 - Region 1 - NRPS,PKS-like,T3PKS,transAT-PKS

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Location: 1 - 100,580 nt. (total: 100,580 nt) Show pHMM detection rules used

Region on contig edge.

transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))
T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)
PKS-like: (t2pks2 or ksIII)
NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 100580:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (gly - ccmal - ohmal - mal) + (mal - ccmal - mal - ohmal - mal) + (mal - ccmal - ohmal - mal) + (X - ccmal - ccmal - ccmal) + (ccmal - mal - mal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 84046:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (gly - ccmal - ohmal - mal) + (mal - ccmal - mal - ohmal - mal) + (mal - ccmal - ohmal - mal) + (X - ccmal - ccmal - ccmal) + (ccmal - mal - mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg72_13

PKS_AT (4..284): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 101.4
Methoxymalonyl-CoA: 55.6
inactive: 37.8
Methylmalonyl-CoA: 37.1
Propionyl-CoA: 24.9
Ethylmalonyl-CoA: 10.8
2-Methylbutyryl-CoA: 9.4
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

ctg72_15

PKS_AT (7..293): pk

ATSignature: (unknown)

No matches above 50%

minowa: inactive

Prediction, score:

inactive: 27.3
Malonyl-CoA: 24.5
Isobutyryl-CoA: 8.5
Benzoyl-CoA: 8.4
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ctg72_16

PKS_AT (4..288): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 66.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 96.2
inactive: 40.7
Methoxymalonyl-CoA: 29.2
Methylmalonyl-CoA: 22.2
Propionyl-CoA: 16.2
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ctg72_22

Search NORINE for peptide: strict or relaxed

CAL_domain (63..471): pk

minowa: fatty_acid

Prediction, score:

fatty_acid: 16.1
NH2: 10.3
shikimic_acid: 0.0
Acetyl-CoA: 0.0
AHBA: 0.0

AMP-binding (1159..1554): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGMIWK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 100% (strong)

ctg72_25

Search NORINE for peptide: strict or relaxed

AMP-binding (497..905): X

NRPSPredictor2: hydrophobic-aliphatic

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVsnmAiIYK
Nearest Stachelhaus code:: ala
Stachelhaus code match:: 60% (weak)

AMCA01000083.1 - Region 1 - NRPS

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Location: 1 - 2,241 nt. (total: 2,241 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

No matches found.

AMCA01000088.1 - Region 1 - NRPS

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Location: 1 - 5,605 nt. (total: 5,605 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 5605:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (leu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg83_2

Search NORINE for peptide: strict or relaxed

AMP-binding (469..867): leu

NRPSPredictor2: leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: leu

Stachelhaus prediction details:

Stachelhaus sequence:: DAFFLGCVFK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 100% (strong)

AMCA01000100.1 - Region 1 - NRPS

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Location: 1 - 10,135 nt. (total: 10,135 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 10135:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (glu - leu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg94_1

Search NORINE for peptide: strict or relaxed

AMP-binding (466..867): glu

NRPSPredictor2: glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: glu, gln
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAKDLGVVDK
Nearest Stachelhaus code:: glu
Stachelhaus code match:: 100% (strong)

AMP-binding (1510..1908): leu

NRPSPredictor2: leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: leu

Stachelhaus prediction details:

Stachelhaus sequence:: DAFMMGMVFK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 100% (strong)

AMCA01000118.1 - Region 1 - T3PKS

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Location: 1 - 25,184 nt. (total: 25,184 nt) Show pHMM detection rules used

Region on contig edge.

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

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KnownClusterBlast

Similar known gene clusters

No matches found.

AMCA01000124.1 - Region 1 - NRPS

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Location: 1 - 12,553 nt. (total: 12,553 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 12553:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (gly - thr)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg116_2

Search NORINE for peptide: strict or relaxed

AMP-binding (31..435): dhb

NRPSPredictor2: dhb

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: dhb, sal
Small clusters prediction:: dhb, sal
Single AA prediction:: dhb

Stachelhaus prediction details:

Stachelhaus sequence:: PLPAQGVVNK
Nearest Stachelhaus code:: dhb
Stachelhaus code match:: 100% (strong)

ctg116_4

Search NORINE for peptide: strict or relaxed

AMP-binding (463..861): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGLIWK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 100% (strong)

AMP-binding (1519..1935): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

AMCA01000190.1 - Region 1 - terpene

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Location: 1 - 14,145 nt. (total: 14,145 nt) Show pHMM detection rules used

Region on contig edge.

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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KnownClusterBlast

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No matches found.

AMCA01000222.1 - Region 1 - lanthipeptide-class-i

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Location: 17,646 - 31,388 nt. (total: 13,743 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-i: ((LANC_like and (Lant_dehydr_N or Lant_dehydr_C)) and not (YcaO or TIGR03882))

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Lanthipeptides

KnownClusterBlast

Lanthipeptide predictions

Lanthipeptide(s) for ctg197_33 - Putative Class I

ctg197_30 leader / core peptide: MSKFDDFDLDVVKVSKQDSKITPQ - WKDhaEDhaLCDhbPGCVDhbGVLQDhbCFLQDhbIDhbCNCKIDhaK

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Similar known gene clusters

Lanthipeptides

Core peptide predictions

ctg197_30 - Class I

Cleavage pHMM score: 27.80

RODEO score: 29

Monoisotopic mass: 3346.7 Da

Molecular weight: 3349.0 Da

Number of bridges: 5

Alternative weights: (assuming N unmodified Ser/Thr residues); 3367.0 Da N = 1; 3385.0 Da N = 2; 3403.0 Da N = 3

AMCA01000223.1 - Region 1 - RRE-containing

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Location: 1 - 12,582 nt. (total: 12,582 nt) Show pHMM detection rules used

Region on contig edge.

RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

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KnownClusterBlast

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No matches found.

AMCA01000296.1 - Region 1 - other

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Location: 1 - 7,390 nt. (total: 7,390 nt) Show pHMM detection rules used

Region on contig edge.

other: (LmbU or Neocarzinostat or fom1 or bcpB or frbD or mitE or vlmB or prnB or CaiA or bacilysin or orf2_PTase)

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AMCA01000301.1 - Region 1 - sactipeptide

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Location: 1 - 11,537 nt. (total: 11,537 nt) Show pHMM detection rules used

Region on contig edge.

sactipeptide: (subtilosin or thuricin or TIGR04404 or Subtilosin_A or skfc)

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KnownClusterBlast

Sactipeptides

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Sactipeptide predictions

Putative Sactipeptide(s)

allorf_01671_01802 leader / core peptide: MKKAVIVENK - GCATCSIGAACLVDGPIPDFEIAGATGLFGLWG

Sactipeptides

Core peptide predictions

allorf_01671_01802: Putative sactipeptide; RODEO score: 28.0; Core sequence, approximate: GCATCSIGAACLVDGPIPDFEIAGATGLFGLWG

AMCA01000316.1 - Region 1 - CDPS

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Location: 5,640 - 26,395 nt. (total: 20,756 nt) Show pHMM detection rules used

CDPS: (CDPS or cycdipepsynth)

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KnownClusterBlast

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No matches found.

AMCA01000327.1 - Region 1 - NRPS

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Location: 1 - 13,606 nt. (total: 13,606 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 13606:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-orn) + (tyr - D-thr)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg284_1

Search NORINE for peptide: strict or relaxed

AMP-binding (358..763): orn

NRPSPredictor2: orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: orn, lys, arg
Small clusters prediction:: orn, horn
Single AA prediction:: orn

Stachelhaus prediction details:

Stachelhaus sequence:: DVGEIGSIDK
Nearest Stachelhaus code:: orn
Stachelhaus code match:: 100% (strong)

ctg284_2

Search NORINE for peptide: strict or relaxed

AMP-binding (474..865): tyr

NRPSPredictor2: tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: phe, trp, phg, tyr, bht
Small clusters prediction:: tyr, bht
Single AA prediction:: tyr

Stachelhaus prediction details:

Stachelhaus sequence:: DGTITAEVAK
Nearest Stachelhaus code:: tyr
Stachelhaus code match:: 100% (strong)

AMP-binding (1505..1903): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

AMCA01000337.1 - Region 1 - terpene

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Location: 1 - 7,293 nt. (total: 7,293 nt) Show pHMM detection rules used

Region on contig edge.

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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KnownClusterBlast

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No matches found.

AMCA01000340.1 - Region 1 - NRPS

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Location: 1 - 7,061 nt. (total: 7,061 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

AMCA01000343.1 - Region 1 - NRPS

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Location: 1 - 9,994 nt. (total: 9,994 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 9994:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-val) + (pro)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg295_1

Search NORINE for peptide: strict or relaxed

AMP-binding (267..660): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: val

Stachelhaus prediction details:

Stachelhaus sequence:: DVFWIGGTFK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 100% (strong)

ctg295_2

Search NORINE for peptide: strict or relaxed

AMP-binding (468..865): pro

NRPSPredictor2: pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: pro, pip
Small clusters prediction:: pro
Single AA prediction:: pro

Stachelhaus prediction details:

Stachelhaus sequence:: DVQFIAHVVK
Nearest Stachelhaus code:: pro
Stachelhaus code match:: 100% (strong)

AMP-binding (1499..1900): glu

NRPSPredictor2: glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: glu, gln
Single AA prediction:: glu

Stachelhaus prediction details:

Stachelhaus sequence:: DAWHFGSVEK
Nearest Stachelhaus code:: glu
Stachelhaus code match:: 100% (strong)