GCA_001281305.1 - 6 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

CP010411.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	lanthipeptide-class-v	1	31,187	N-octanoyl-Met-Phe-H	NRP	22%
Region 2	NRPS-like	154,267	197,278	nosiheptide	RiPP:Thiopeptide	7%
Region 3	RiPP-like	1,246,807	1,255,851
Region 4	lanthipeptide-class-ii	1,255,985	1,279,065
Region 5	lanthipeptide-class-iii,RRE-containing	1,313,087	1,342,743
Region 6	RiPP-like	2,415,441	2,425,833

CP010411.1 - Region 1 - lanthipeptide-class-v

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Location: 1 - 31,187 nt. (total: 31,187 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-v: (APH and HopA1)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

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Gene details

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Lanthipeptides

KnownClusterBlast

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Similar known gene clusters

Lanthipeptides

Core peptide predictions

No core peptides found.

CP010411.1 - Region 2 - NRPS-like

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Location: 154,267 - 197,278 nt. (total: 43,012 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 2, location 154266 - 197278:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg1_139

Search NORINE for peptide: strict or relaxed

AMP-binding (14..381): X

NRPSPredictor2: gly, ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DfftIGiIYK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 60% (weak)

CP010411.1 - Region 3 - RiPP-like

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Location: 1,246,807 - 1,255,851 nt. (total: 9,045 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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KnownClusterBlast

Similar known gene clusters

No matches found.

CP010411.1 - Region 4 - lanthipeptide-class-ii

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Location: 1,255,985 - 1,279,065 nt. (total: 23,081 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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Gene details

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Lanthipeptides

KnownClusterBlast

Lanthipeptide predictions

Lanthipeptide(s) for ctg1_1040 - Putative Class II

ctg1_1033 leader / core peptide: MVTLKNDNVVGDRGDGAAAA - VGDhaARELVDGLVDhaDhaGRWIPCDhaDhaG
allorf_1259724_1259795 leader / core peptide: MPPARTAAASGM - DhaDhbIGVCMAQYE
allorf_1259774_1259971 leader / core peptide: VHGPIRVIRRDGVTLGG - PQHAQHEQRGRGGKPVDQHAARFPQFQRDhaIAHIDhaYPRLRDhaAYIACVDhaV
allorf_1270034_1270174 leader / core peptide: MRHYDAVAGS - RLPDAICRLDhaFIGCRLDhaDADhaVDhaAQRCDDhaVPVRADhaIV
allorf_1270208_1270282 leader / core peptide: LRKRVFFA - YWDNDhaRCPGLRVNRDhaDhb
allorf_1259634_1259786 leader / core peptide: LGHAHTNRAHAAGG - GRPCRWHRAGLAVREDhbRMGDGRDhaACPRVAAGDhaCRRG
allorf_1265737_1265847 leader / core peptide: LQEQTKVPHS - CVRVQDhbHPLLAMYGLDhbILVNDhaLDhaICF

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Similar known gene clusters

No matches found.

Lanthipeptides

Core peptide predictions

ctg1_1033 - Class II

Cleavage pHMM score: -6.00

RODEO score: 0

Monoisotopic mass: 2241.2 Da

Molecular weight: 2242.5 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2260.5 Da N = 1; 2278.5 Da N = 2; 2296.6 Da N = 3; 2314.6 Da N = 4

allorf_1259724_1259795 - Class II

Cleavage pHMM score: -7.30

RODEO score: 0

Monoisotopic mass: 1164.5 Da

Molecular weight: 1165.3 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1183.3 Da N = 1

allorf_1259774_1259971 - Class II

Cleavage pHMM score: -3.70

RODEO score: 0

Monoisotopic mass: 5377.8 Da

Molecular weight: 5381.0 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 5399.0 Da N = 1; 5417.0 Da N = 2; 5435.1 Da N = 3

allorf_1270034_1270174 - Class II

Cleavage pHMM score: -3.90

RODEO score: 0

Monoisotopic mass: 3752.0 Da

Molecular weight: 3754.3 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 3772.4 Da N = 1; 3790.4 Da N = 2; 3808.4 Da N = 3

allorf_1270208_1270282 - Class II

Cleavage pHMM score: -6.30

RODEO score: 0

Monoisotopic mass: 1868.9 Da

Molecular weight: 1870.0 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1888.1 Da N = 1; 1906.1 Da N = 2

allorf_1259634_1259786 - Class II

Cleavage pHMM score: -4.40

RODEO score: 0

Monoisotopic mass: 3853.9 Da

Molecular weight: 3856.4 Da

Number of bridges: 3

allorf_1265737_1265847 - Class II

Cleavage pHMM score: -7.20

RODEO score: 0

Monoisotopic mass: 2818.5 Da

Molecular weight: 2820.4 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 2838.4 Da N = 1; 2856.5 Da N = 2

CP010411.1 - Region 5 - RRE-containing,lanthipeptide-class-iii

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Location: 1,313,087 - 1,342,743 nt. (total: 29,657 nt) Show pHMM detection rules used

lanthipeptide-class-iii: (micKC and not PKS_KS)
RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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Lanthipeptides

KnownClusterBlast

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Similar known gene clusters

No matches found.

Lanthipeptides

Core peptide predictions

No core peptides found.

CP010411.1 - Region 6 - RiPP-like

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Location: 2,415,441 - 2,425,833 nt. (total: 10,393 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

KnownClusterBlast

Similar known gene clusters

No matches found.