GCA_001587435.1 - 14 region(s)

Identified secondary metabolite regions using strictness 'relaxed'

LQYP01000010.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 2.1	NRPS	1	27,388	surfactin	NRP:Lipopeptide	47%

LQYP01000030.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 3.1	NRPS-like	1	1,638

LQYP01000002.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 10.1	NRPS,transAT-PKS,betalactone	32,641	142,894	fengycin	NRP	93%

LQYP01000018.1

Region	Type	From	To
Region 11.1	NRPS	189,303	234,830
Region 11.2	T3PKS	581,585	622,685
Region 11.3	terpene	677,908	699,791

LQYP01000019.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 12.1	transAT-PKS,T3PKS,NRPS	80,434	183,083	bacillaene	Polyketide + NRP	100%

LQYP01000003.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 13.1	other	193,468	234,886	bacilysin	Other	100%
Region 13.2	RiPP-like,NRPS	750,318	802,093	bacillibactin	NRP	100%

LQYP01000025.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 20.1	NRPS	1	25,285	surfactin	NRP:Lipopeptide	39%

LQYP01000008.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 22.1	lanthipeptide-class-iii	42,957	55,875

LQYP01000027.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 23.1	PKS-like	104,874	146,118	butirosin A / butirosin B	Saccharide	7%
Region 23.2	terpene	230,838	251,581

LQYP01000009.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 25.1	NRPS	1	9,237	surfactin	NRP:Lipopeptide	8%

Region	Type	From	To	Most similar known cluster		Similarity
Region 2.1	NRPS	1	27,388	surfactin	NRP:Lipopeptide	47%
Region 3.1	NRPS-like	1	1,638
Region 10.1	NRPS,transAT-PKS,betalactone	32,641	142,894	fengycin	NRP	93%
Region 11.1	NRPS	189,303	234,830
Region 11.2	T3PKS	581,585	622,685
Region 11.3	terpene	677,908	699,791
Region 12.1	transAT-PKS,T3PKS,NRPS	80,434	183,083	bacillaene	Polyketide + NRP	100%
Region 13.1	other	193,468	234,886	bacilysin	Other	100%
Region 13.2	RiPP-like,NRPS	750,318	802,093	bacillibactin	NRP	100%
Region 20.1	NRPS	1	25,285	surfactin	NRP:Lipopeptide	39%
Region 22.1	lanthipeptide-class-iii	42,957	55,875
Region 23.1	PKS-like	104,874	146,118	butirosin A / butirosin B	Saccharide	7%
Region 23.2	terpene	230,838	251,581
Region 25.1	NRPS	1	9,237	surfactin	NRP:Lipopeptide	8%

Compact view

LQYP01000010.1 - Region 1 - NRPS

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Location: 1 - 27,388 nt. (total: 27,388 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 27388:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (glu - leu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

LQYP01000030.1 - Region 1 - NRPS-like

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Location: 1 - 1,638 nt. (total: 1,638 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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core biosynthetic genes

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 1638:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (leu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

LQYP01000002.1 - Region 1 - NRPS,betalactone,transAT-PKS

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Location: 32,641 - 142,894 nt. (total: 110,254 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))
transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))
betalactone: (HMGL-like and AMP-binding)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 32640 - 142894:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (glu - D-tyr) + (ile) + (mal - asn) + (D-tyr - D-asn - gln - pro) + (D-asn - ser)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 32640 - 109732:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal - asn) + (D-tyr - D-asn - gln - pro) + (D-asn - ser)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 91405 - 142894:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (glu - D-tyr) + (ile)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

LQYP01000018.1 - Region 1 - NRPS

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Location: 189,303 - 234,830 nt. (total: 45,528 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 189302 - 234830:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

LQYP01000018.1 - Region 2 - T3PKS

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Location: 581,585 - 622,685 nt. (total: 41,101 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

Legend:

core biosynthetic genes

additional biosynthetic genes

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KnownClusterBlast

Similar known gene clusters

No matches found.

LQYP01000018.1 - Region 3 - terpene

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Location: 677,908 - 699,791 nt. (total: 21,884 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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resistance

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Gene details

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KnownClusterBlast

Similar known gene clusters

No matches found.

LQYP01000019.1 - Region 1 - NRPS,T3PKS,transAT-PKS

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Location: 80,434 - 183,083 nt. (total: 102,650 nt) Show pHMM detection rules used

transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))
T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)
NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 80433 - 183083:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (gly - ccmal - ohmal - mal) + (mal - ccmal - mal - ohmal - mal) + (mal - ccmal - ohmal) + (X - ccmal - ccmal - ccmal) + (ccmal - mal - mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg12_140

PKS_AT (4..285): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 98.2
inactive: 43.3
Methoxymalonyl-CoA: 36.6
Methylmalonyl-CoA: 24.6
Propionyl-CoA: 24.5
2-Methylbutyryl-CoA: 5.7
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

ctg12_141

PKS_AT (7..293): mal

ATSignature: (unknown)

No matches above 50%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 29.5
inactive: 17.6
Methoxymalonyl-CoA: 11.9
Benzoyl-CoA: 9.4
Isobutyryl-CoA: 8.6
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ctg12_142

PKS_AT (4..282): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 66.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 99.9
inactive: 30.9
Methoxymalonyl-CoA: 28.6
Methylmalonyl-CoA: 15.3
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ctg12_147

Search NORINE for peptide: strict or relaxed

CAL_domain (74..476): pk

minowa: fatty_acid

Prediction, score:

fatty_acid: 22.2
shikimic_acid: 0.0
NH2: 0.0
Acetyl-CoA: 0.0
AHBA: 0.0

AMP-binding (1145..1540): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGMIWK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 100% (strong)

ctg12_150

Search NORINE for peptide: strict or relaxed

AMP-binding (494..898): X

NRPSPredictor2: hydrophobic-aliphatic

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVsnmAiIYK
Nearest Stachelhaus code:: ala
Stachelhaus code match:: 60% (weak)

LQYP01000003.1 - Region 1 - other

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Location: 193,468 - 234,886 nt. (total: 41,419 nt) Show pHMM detection rules used

other: (LmbU or Neocarzinostat or fom1 or bcpB or frbD or mitE or vlmB or prnB or CaiA or bacilysin or orf2_PTase)

Legend:

core biosynthetic genes

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KnownClusterBlast

Similar known gene clusters

LQYP01000003.1 - Region 2 - NRPS,RiPP-like

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Location: 750,318 - 802,093 nt. (total: 51,776 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193)
NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 2, location 750317 - 802093:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (gly - thr)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 754965 - 802093:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (gly - thr)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

LQYP01000025.1 - Region 1 - NRPS

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Location: 1 - 25,285 nt. (total: 25,285 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 25285:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ile)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

LQYP01000008.1 - Region 1 - lanthipeptide-class-iii

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Location: 42,957 - 55,875 nt. (total: 12,919 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-iii: (micKC and not PKS_KS)

Legend:

core biosynthetic genes

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Lanthipeptides

KnownClusterBlast

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Lanthipeptides

Core peptide predictions

No core peptides found.

LQYP01000027.1 - Region 1 - PKS-like

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Location: 104,874 - 146,118 nt. (total: 41,245 nt) Show pHMM detection rules used

PKS-like: (t2pks2 or ksIII)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

LQYP01000027.1 - Region 2 - terpene

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Location: 230,838 - 251,581 nt. (total: 20,744 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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KnownClusterBlast

Similar known gene clusters

No matches found.

LQYP01000009.1 - Region 1 - NRPS

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Location: 1 - 9,237 nt. (total: 9,237 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

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Gene details

Select a gene to view the details available for it

NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 9237:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (val - asp)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form