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Overview
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1.5
1.6
1.7
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1.10
1.11
1.12
1.13

### Identified secondary metabolite regions using strictness 'relaxed'

CP022391.1

Region | Type | From | To | Most similar known cluster | Similarity
--- | --- | --- | --- | --- | ---
Region 1 | ranthipeptide,sactipeptide | 204,175 | 226,248 | sporulation killing factor | RiPP:Head-to-tailcyclized peptide | 100%
Region 2 | NRPS | 358,303 | 421,744 | surfactin | NRP:Lipopeptide | 82%
Region 3 | terpene | 1,149,958 | 1,170,476 |
Region 4 | transAT-PKS,PKS-like,T3PKS,NRPS | 1,763,763 | 1,869,009 | bacillaene | Polyketide + NRP | 100%
Region 5 | NRPS,betalactone | 1,940,625 | 2,017,660 | fengycin | NRP | 100%
Region 6 | terpene | 2,092,168 | 2,114,066 |
Region 7 | T3PKS | 2,296,956 | 2,338,053 |
Region 8 | NRPS | 3,260,519 | 3,307,655 | bacillibactin | NRP | 100%
Region 9 | CDPS | 3,593,821 | 3,614,567 |
Region 10 | sactipeptide | 3,826,058 | 3,847,669 | subtilosin A | RiPP:Thiopeptide | 100%
Region 11 | other | 3,850,668 | 3,892,086 | bacilysin | Other | 100%
Region 12 | RRE-containing | 4,088,150 | 4,108,419 |
Region 13 | epipeptide | 4,115,742 | 4,137,440 | thailanstatin A | NRP + Polyketide | 10%

CP022391.1 - Region 1 - ranthipeptide,sactipeptide
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 204,175 - 226,248 nt. (total: 22,074 nt) Show pHMM detection rules used
ranthipeptide: (TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085 or SPASM)) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716 or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or YcaO or TIGR03882)))
sactipeptide: (subtilosin or thuricin or TIGR04404 or Subtilosin_A or skfc)

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
reset view
zoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
KnownClusterBlast
Sactipeptides
Similar known gene clusters
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.
Sactipeptide predictions
Lists the possible core peptides for each biosynthetic enzyme, including the predicted class. Each core peptide shows the leader and core peptide sequences, separated by a dash.
Sactipeptides - No core peptides found.
Sactipeptides
Core peptide predictions
Lists the possible core peptides in the region. Each core peptide lists its RODEO score and predicted core sequence.
No core peptides found.
CP022391.1 - Region 2 - NRPS
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 358,303 - 421,744 nt. (total: 63,442 nt) Show pHMM detection rules used
NRPS: cds(Condensation and (AMP-binding or A-OX))

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
reset view
zoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
NRPS/PKS domains
KnownClusterBlast
Detailed domain annotation
Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
A glossary is available here.
Selected features only
Show module domains
Similar known gene clusters
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.
NRPS/PKS products
NRPS/PKS monomers
Predicted core structure(s)
Shows estimated product structure and polymer for each candidate cluster in the region. To show the product, click on the expander or the candidate cluster feature drawn in the overview.
For candidate cluster 2, location 358302 - 421744:
Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:
  (glu - leu - D-leu) + (val - asp - D-leu) + (leu)

Direct lookup in NORINE database: strict or relaxed
Link to NORINE database query form
NRPS/PKS monomer predictions
Shows the predicted monomers for each adynelation domain and acyltransferase within genes. Each gene prediction can be expanded to view detailed predictions of each domain. Each prediction can be expanded to view the predictions by tool (and, for some tools, further expanded for extra details).

ctg1_356: glu - leu - leu
  Search NORINE for peptide: strict or relaxed
  AMP-binding (466..867): glu
  NRPSPredictor2: glu
  SVM prediction details:
    Predicted physicochemical class:
      hydrophilic
    Large clusters prediction:
      asp, asn, glu, gln, aad
    Small clusters prediction:
      glu, gln
    Single AA prediction:
      N/A
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DAKDLGVVDK
    Nearest Stachelhaus code:
      glu
    Stachelhaus code match:
      100% (strong)
  AMP-binding (1510..1908): leu
  NRPSPredictor2: leu
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aliphatic
    Large clusters prediction:
      gly, ala, val, leu, ile, abu, iva
    Small clusters prediction:
      val, leu, ile, abu, iva
    Single AA prediction:
      leu
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DAFMMGMVFK
    Nearest Stachelhaus code:
      leu
    Stachelhaus code match:
      100% (strong)
  AMP-binding (2550..2938): leu
  NRPSPredictor2: leu
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aliphatic
    Large clusters prediction:
      gly, ala, val, leu, ile, abu, iva
    Small clusters prediction:
      val, leu, ile, abu, iva
    Single AA prediction:
      leu
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DAWFLGNVVK
    Nearest Stachelhaus code:
      leu
    Stachelhaus code match:
      100% (strong)
ctg1_357: val - asp - leu
  Search NORINE for peptide: strict or relaxed
  AMP-binding (470..864): val
  NRPSPredictor2: val
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aliphatic
    Large clusters prediction:
      gly, ala, val, leu, ile, abu, iva
    Small clusters prediction:
      val, leu, ile, abu, iva
    Single AA prediction:
      val
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DAFWIGGTFK
    Nearest Stachelhaus code:
      val
    Stachelhaus code match:
      100% (strong)
  AMP-binding (1501..1904): asp
  NRPSPredictor2: asp
  SVM prediction details:
    Predicted physicochemical class:
      hydrophilic
    Large clusters prediction:
      asp, asn, glu, gln, aad
    Small clusters prediction:
      asp, asn
    Single AA prediction:
      asp
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DLTKVGHIGK
    Nearest Stachelhaus code:
      asp
    Stachelhaus code match:
      100% (strong)
  AMP-binding (2546..2934): leu
  NRPSPredictor2: leu
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aliphatic
    Large clusters prediction:
      gly, ala, val, leu, ile, abu, iva
    Small clusters prediction:
      val, leu, ile, abu, iva
    Single AA prediction:
      leu
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DAWFLGNVVK
    Nearest Stachelhaus code:
      leu
    Stachelhaus code match:
      100% (strong)
ctg1_358: leu
  Search NORINE for peptide: strict or relaxed
  AMP-binding (469..867): leu
  NRPSPredictor2: leu
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aliphatic
    Large clusters prediction:
      gly, ala, val, leu, ile, abu, iva
    Small clusters prediction:
      val, leu, ile, abu, iva
    Single AA prediction:
      leu
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DAFFLGCVFK
    Nearest Stachelhaus code:
      leu
    Stachelhaus code match:
      100% (strong)

CP022391.1 - Region 3 - terpene
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 1,149,958 - 1,170,476 nt. (total: 20,519 nt) Show pHMM detection rules used
terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
reset view
zoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
KnownClusterBlast
Similar known gene clusters
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.

No matches found.

CP022391.1 - Region 4 - NRPS,PKS-like,T3PKS,transAT-PKS
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 1,763,763 - 1,869,009 nt. (total: 105,247 nt) Show pHMM detection rules used
transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))
PKS-like: (t2pks2 or ksIII)
T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)
NRPS: cds(Condensation and (AMP-binding or A-OX))

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
reset view
zoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
NRPS/PKS domains
KnownClusterBlast
Detailed domain annotation
Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
A glossary is available here.
Selected features only
Show module domains
Similar known gene clusters
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.
NRPS/PKS products
NRPS/PKS monomers
Predicted core structure(s)
Shows estimated product structure and polymer for each candidate cluster in the region. To show the product, click on the expander or the candidate cluster feature drawn in the overview.
For candidate cluster 4, location 1763762 - 1869009:
Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:
  (gly - ccmal - ohmal - mal) + (mal - ccmal - mal - ohmal - mal) + (mal - ccmal - ohmal - mal) + (X - ccmal - ccmal - ccmal) + (ccmal - mal - mal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 5, location 1763762 - 1854341:
Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:
  (gly - ccmal - ohmal - mal) + (mal - ccmal - mal - ohmal - mal) + (mal - ccmal - ohmal - mal) + (X - ccmal - ccmal - ccmal) + (ccmal - mal - mal)

Direct lookup in NORINE database: strict or relaxed
Link to NORINE database query form
NRPS/PKS monomer predictions
Shows the predicted monomers for each adynelation domain and acyltransferase within genes. Each gene prediction can be expanded to view detailed predictions of each domain. Each prediction can be expanded to view the predictions by tool (and, for some tools, further expanded for extra details).

ctg1_1709: mal
  PKS_AT (4..284): mal
  ATSignature: Malonyl-CoA
  Top 3 matches:
    Malonyl-CoA: 87.5%
    inactive: 62.5%
  minowa: Malonyl-CoA
  Prediction, score:
    Malonyl-CoA: 98.2
    Methoxymalonyl-CoA: 54.0
    inactive: 36.8
    Methylmalonyl-CoA: 35.6
    Propionyl-CoA: 22.9
    Ethylmalonyl-CoA: 10.8
    2-Methylbutyryl-CoA: 9.4
    Isobutyryl-CoA: 9.1
    trans-1,2-CPDA: 0.0
    fatty_acid: 0.0
    CHC-CoA: 0.0
    Benzoyl-CoA: 0.0
    Acetyl-CoA: 0.0
    3-Methylbutyryl-CoA: 0.0
ctg1_1711: pk
  PKS_AT (7..293): pk
  ATSignature: (unknown)
  No matches above 50%
  minowa: inactive
  Prediction, score:
    inactive: 30.1
    Malonyl-CoA: 24.5
    Benzoyl-CoA: 8.7
    Isobutyryl-CoA: 8.6
    trans-1,2-CPDA: 0.0
    fatty_acid: 0.0
    Propionyl-CoA: 0.0
    Methylmalonyl-CoA: 0.0
    Methoxymalonyl-CoA: 0.0
    Ethylmalonyl-CoA: 0.0
    CHC-CoA: 0.0
    Acetyl-CoA: 0.0
    3-Methylbutyryl-CoA: 0.0
    2-Methylbutyryl-CoA: 0.0
ctg1_1712: mal
  PKS_AT (4..288): mal
  ATSignature: Malonyl-CoA
  Top 3 matches:
    Malonyl-CoA: 83.3%
    inactive: 66.7%
  minowa: Malonyl-CoA
  Prediction, score:
    Malonyl-CoA: 91.4
    inactive: 40.0
    Methoxymalonyl-CoA: 25.7
    Methylmalonyl-CoA: 17.4
    Propionyl-CoA: 12.8
    trans-1,2-CPDA: 0.0
    fatty_acid: 0.0
    Isobutyryl-CoA: 0.0
    Ethylmalonyl-CoA: 0.0
    CHC-CoA: 0.0
    Benzoyl-CoA: 0.0
    Acetyl-CoA: 0.0
    3-Methylbutyryl-CoA: 0.0
    2-Methylbutyryl-CoA: 0.0
ctg1_1718: pk - gly
  Search NORINE for peptide: strict or relaxed
  CAL_domain (60..476): pk
  minowa: fatty_acid
  Prediction, score:
    fatty_acid: 16.2
    NH2: 10.3
    shikimic_acid: 0.0
    Acetyl-CoA: 0.0
    AHBA: 0.0
  AMP-binding (1159..1554): gly
  NRPSPredictor2: gly
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aliphatic
    Large clusters prediction:
      gly, ala, val, leu, ile, abu, iva
    Small clusters prediction:
      gly, ala
    Single AA prediction:
      gly
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DILQLGMIWK
    Nearest Stachelhaus code:
      gly
    Stachelhaus code match:
      100% (strong)
ctg1_1721: X
  Search NORINE for peptide: strict or relaxed
  AMP-binding (497..905): X
  NRPSPredictor2: hydrophobic-aliphatic
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aliphatic
    Large clusters prediction:
      N/A
    Small clusters prediction:
      N/A
    Single AA prediction:
      N/A
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DVsnmAiIYK
    Nearest Stachelhaus code:
      ala
    Stachelhaus code match:
      60% (weak)

CP022391.1 - Region 5 - NRPS,betalactone
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 1,940,625 - 2,017,660 nt. (total: 77,036 nt) Show pHMM detection rules used
NRPS: cds(Condensation and (AMP-binding or A-OX))
betalactone: (HMGL-like and AMP-binding)

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
reset view
zoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
NRPS/PKS domains
KnownClusterBlast
Detailed domain annotation
Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
A glossary is available here.
Selected features only
Show module domains
Similar known gene clusters
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.
NRPS/PKS products
NRPS/PKS monomers
Predicted core structure(s)
Shows estimated product structure and polymer for each candidate cluster in the region. To show the product, click on the expander or the candidate cluster feature drawn in the overview.
For candidate cluster 6, location 1940624 - 2017660:
Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:
  (glu - D-orn) + (tyr - D-thr) + (glu - D-val) + (pro - glu - D-tyr) + (ile)

Direct lookup in NORINE database: strict or relaxed
Link to NORINE database query form
NRPS/PKS monomer predictions
Shows the predicted monomers for each adynelation domain and acyltransferase within genes. Each gene prediction can be expanded to view detailed predictions of each domain. Each prediction can be expanded to view the predictions by tool (and, for some tools, further expanded for extra details).

ctg1_1834: X
  Search NORINE for peptide: strict or relaxed
  AMP-binding (13..445): X
  NRPSPredictor2: (unknown)
  SVM prediction details:
    NOTE: outside applicability domain
    Predicted physicochemical class:
      N/A
    Large clusters prediction:
      asp, asn, glu, gln, aad
    Small clusters prediction:
      N/A
    Single AA prediction:
      N/A
  Stachelhaus prediction details:
    Stachelhaus sequence:
      gclhIAApvK
    Nearest Stachelhaus code:
      phe
    Stachelhaus code match:
      40% (weak)
ctg1_1839: ile
  Search NORINE for peptide: strict or relaxed
  AMP-binding (474..867): ile
  NRPSPredictor2: ile
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aliphatic
    Large clusters prediction:
      gly, ala, val, leu, ile, abu, iva
    Small clusters prediction:
      val, leu, ile, abu, iva
    Single AA prediction:
      ile
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DAFFYGITFK
    Nearest Stachelhaus code:
      ile
    Stachelhaus code match:
      100% (strong)
ctg1_1840: pro - glu - tyr
  Search NORINE for peptide: strict or relaxed
  AMP-binding (468..865): pro
  NRPSPredictor2: pro
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aliphatic
    Large clusters prediction:
      pro, pip
    Small clusters prediction:
      pro
    Single AA prediction:
      pro
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DVQFIAHVVK
    Nearest Stachelhaus code:
      pro
    Stachelhaus code match:
      100% (strong)
  AMP-binding (1499..1900): glu
  NRPSPredictor2: glu
  SVM prediction details:
    Predicted physicochemical class:
      hydrophilic
    Large clusters prediction:
      asp, asn, glu, gln, aad
    Small clusters prediction:
      glu, gln
    Single AA prediction:
      glu
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DAWHFGSVEK
    Nearest Stachelhaus code:
      glu
    Stachelhaus code match:
      100% (strong)
  AMP-binding (2538..2932): tyr
  NRPSPredictor2: tyr
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aromatic
    Large clusters prediction:
      phe, trp, phg, tyr, bht
    Small clusters prediction:
      tyr, bht
    Single AA prediction:
      tyr
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DGTITAEVAK
    Nearest Stachelhaus code:
      tyr
    Stachelhaus code match:
      100% (strong)
ctg1_1841: glu - val
  Search NORINE for peptide: strict or relaxed
  AMP-binding (469..870): glu
  NRPSPredictor2: glu
  SVM prediction details:
    Predicted physicochemical class:
      hydrophilic
    Large clusters prediction:
      asp, asn, glu, gln, aad
    Small clusters prediction:
      glu, gln
    Single AA prediction:
      glu
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DAWHFGGVDK
    Nearest Stachelhaus code:
      glu
    Stachelhaus code match:
      100% (strong)
  AMP-binding (1510..1903): val
  NRPSPredictor2: val
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aliphatic
    Large clusters prediction:
      gly, ala, val, leu, ile, abu, iva
    Small clusters prediction:
      val, leu, ile, abu, iva
    Single AA prediction:
      val
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DVFWIGGTFK
    Nearest Stachelhaus code:
      val
    Stachelhaus code match:
      100% (strong)
ctg1_1842: tyr - thr
  Search NORINE for peptide: strict or relaxed
  AMP-binding (474..865): tyr
  NRPSPredictor2: tyr
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aromatic
    Large clusters prediction:
      phe, trp, phg, tyr, bht
    Small clusters prediction:
      tyr, bht
    Single AA prediction:
      tyr
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DGTITAEVAK
    Nearest Stachelhaus code:
      tyr
    Stachelhaus code match:
      100% (strong)
  AMP-binding (1505..1903): thr
  NRPSPredictor2: thr
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aliphatic
    Large clusters prediction:
      ser, thr, dhpg, hpg
    Small clusters prediction:
      thr
    Single AA prediction:
      thr
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DFWNIGMVHK
    Nearest Stachelhaus code:
      thr
    Stachelhaus code match:
      100% (strong)
ctg1_1843: glu - orn
  Search NORINE for peptide: strict or relaxed
  AMP-binding (463..864): glu
  NRPSPredictor2: glu
  SVM prediction details:
    Predicted physicochemical class:
      hydrophilic
    Large clusters prediction:
      asp, asn, glu, gln, aad
    Small clusters prediction:
      glu, gln
    Single AA prediction:
      glu
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DAWHFGGVDK
    Nearest Stachelhaus code:
      glu
    Stachelhaus code match:
      100% (strong)
  AMP-binding (1503..1908): orn
  NRPSPredictor2: orn
  SVM prediction details:
    Predicted physicochemical class:
      hydrophilic
    Large clusters prediction:
      orn, lys, arg
    Small clusters prediction:
      orn, horn
    Single AA prediction:
      orn
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DVGEIGSIDK
    Nearest Stachelhaus code:
      orn
    Stachelhaus code match:
      100% (strong)

CP022391.1 - Region 6 - terpene
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 2,092,168 - 2,114,066 nt. (total: 21,899 nt) Show pHMM detection rules used
terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
reset view
zoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
KnownClusterBlast
Similar known gene clusters
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.

No matches found.

CP022391.1 - Region 7 - T3PKS
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 2,296,956 - 2,338,053 nt. (total: 41,098 nt) Show pHMM detection rules used
T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
reset view
zoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
KnownClusterBlast
Similar known gene clusters
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.

No matches found.

CP022391.1 - Region 8 - NRPS
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 3,260,519 - 3,307,655 nt. (total: 47,137 nt) Show pHMM detection rules used
NRPS: cds(Condensation and (AMP-binding or A-OX))

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
reset view
zoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
NRPS/PKS domains
KnownClusterBlast
Detailed domain annotation
Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
A glossary is available here.
Selected features only
Show module domains
Similar known gene clusters
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.
NRPS/PKS products
NRPS/PKS monomers
Predicted core structure(s)
Shows estimated product structure and polymer for each candidate cluster in the region. To show the product, click on the expander or the candidate cluster feature drawn in the overview.
For candidate cluster 9, location 3260518 - 3307655:
Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:
  (gly - thr)

Direct lookup in NORINE database: strict or relaxed
Link to NORINE database query form
NRPS/PKS monomer predictions
Shows the predicted monomers for each adynelation domain and acyltransferase within genes. Each gene prediction can be expanded to view detailed predictions of each domain. Each prediction can be expanded to view the predictions by tool (and, for some tools, further expanded for extra details).

ctg1_3052: gly - thr
  Search NORINE for peptide: strict or relaxed
  AMP-binding (463..861): gly
  NRPSPredictor2: gly
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aliphatic
    Large clusters prediction:
      gly, ala, val, leu, ile, abu, iva
    Small clusters prediction:
      gly, ala
    Single AA prediction:
      gly
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DILQLGLIWK
    Nearest Stachelhaus code:
      gly
    Stachelhaus code match:
      100% (strong)
  AMP-binding (1519..1935): thr
  NRPSPredictor2: thr
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aliphatic
    Large clusters prediction:
      ser, thr, dhpg, hpg
    Small clusters prediction:
      thr
    Single AA prediction:
      thr
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DFWNIGMVHK
    Nearest Stachelhaus code:
      thr
    Stachelhaus code match:
      100% (strong)
ctg1_3054: dhb
  Search NORINE for peptide: strict or relaxed
  AMP-binding (31..435): dhb
  NRPSPredictor2: dhb
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aromatic
    Large clusters prediction:
      dhb, sal
    Small clusters prediction:
      dhb, sal
    Single AA prediction:
      dhb
  Stachelhaus prediction details:
    Stachelhaus sequence:
      PLPAQGVVNK
    Nearest Stachelhaus code:
      dhb
    Stachelhaus code match:
      100% (strong)

CP022391.1 - Region 9 - CDPS
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 3,593,821 - 3,614,567 nt. (total: 20,747 nt) Show pHMM detection rules used
CDPS: (CDPS or cycdipepsynth)

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
reset view
zoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
KnownClusterBlast
Similar known gene clusters
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.

No matches found.

CP022391.1 - Region 10 - sactipeptide
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 3,826,058 - 3,847,669 nt. (total: 21,612 nt) Show pHMM detection rules used
sactipeptide: (subtilosin or thuricin or TIGR04404 or Subtilosin_A or skfc)

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
reset view
zoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
KnownClusterBlast
Sactipeptides
Similar known gene clusters
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.
Sactipeptide predictions
Lists the possible core peptides for each biosynthetic enzyme, including the predicted class. Each core peptide shows the leader and core peptide sequences, separated by a dash.

#### Putative Sactipeptide(s)

ctg1_3599 leader / core peptide
  MKKAVIVENK - GCATCSIGAACLVDGPIPDFEIAGATGLFGLWG

Sactipeptides
Core peptide predictions
Lists the possible core peptides in the region. Each core peptide lists its RODEO score and predicted core sequence.

ctg1_3599
  Putative sactipeptide
  RODEO score: 33.0
  Core sequence, approximate: GCATCSIGAACLVDGPIPDFEIAGATGLFGLWG

CP022391.1 - Region 11 - other
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 3,850,668 - 3,892,086 nt. (total: 41,419 nt) Show pHMM detection rules used
other: (LmbU or Neocarzinostat or fom1 or bcpB or frbD or mitE or vlmB or prnB or CaiA or bacilysin or orf2_PTase)

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
reset view
zoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
KnownClusterBlast
Similar known gene clusters
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.
CP022391.1 - Region 12 - RRE-containing
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 4,088,150 - 4,108,419 nt. (total: 20,270 nt) Show pHMM detection rules used
RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
reset view
zoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
KnownClusterBlast
Similar known gene clusters
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.

No matches found.

CP022391.1 - Region 13 - epipeptide
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 4,115,742 - 4,137,440 nt. (total: 21,699 nt) Show pHMM detection rules used
epipeptide: (TIGR04078 and yydH)

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
reset view
zoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
KnownClusterBlast
Similar known gene clusters
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.