GCA_002224045.1 - 14 region(s)

Identified secondary metabolite regions using strictness 'relaxed'

NMRK01000014.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 5.1	NRPS	1	29,916

NMRK01000017.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 8.1	NRPS	1	9,869	surfactin	NRP:Lipopeptide	8%

NMRK01000001.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 11.1	transAT-PKS,NRPS-like,NRPS,T3PKS	36,507	137,267	bacillaene	Polyketide + NRP	100%
Region 11.2	terpene	699,510	720,253
Region 11.3	PKS-like	804,950	846,194	butirosin A / butirosin B	Saccharide	7%

NMRK01000021.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 13.1	NRPS-like	1	1,151	icosalide A / icosalide B	NRP:Lipopeptide	100%

NMRK01000002.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 14.1	other	193,511	234,929	bacilysin	Other	100%
Region 14.2	RiPP-like,NRPS	787,771	837,808	bacillibactin	NRP	100%

NMRK01000003.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 15.1	terpene	16,887	38,770
Region 15.2	T3PKS	93,989	135,089

NMRK01000006.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 18.1	NRPS	1	28,208	surfactin	NRP:Lipopeptide	56%

NMRK01000007.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 19.1	NRPS	1	25,538	surfactin	NRP:Lipopeptide	39%

NMRK01000008.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 20.1	NRPS,transAT-PKS,betalactone	32,598	142,854	fengycin	NRP	93%

NMRK01000009.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 21.1	lanthipeptide-class-iii	55,153	77,750

Region	Type	From	To	Most similar known cluster	Similarity
Region 5.1	NRPS	1	29,916
Region 8.1	NRPS	1	9,869	surfactin	NRP:Lipopeptide	8%
Region 11.1	transAT-PKS,NRPS-like,NRPS,T3PKS	36,507	137,267	bacillaene	Polyketide + NRP	100%
Region 11.2	terpene	699,510	720,253
Region 11.3	PKS-like	804,950	846,194	butirosin A / butirosin B	Saccharide	7%
Region 13.1	NRPS-like	1	1,151	icosalide A / icosalide B	NRP:Lipopeptide	100%
Region 14.1	other	193,511	234,929	bacilysin	Other	100%
Region 14.2	RiPP-like,NRPS	787,771	837,808	bacillibactin	NRP	100%
Region 15.1	terpene	16,887	38,770
Region 15.2	T3PKS	93,989	135,089
Region 18.1	NRPS	1	28,208	surfactin	NRP:Lipopeptide	56%
Region 19.1	NRPS	1	25,538	surfactin	NRP:Lipopeptide	39%
Region 20.1	NRPS,transAT-PKS,betalactone	32,598	142,854	fengycin	NRP	93%
Region 21.1	lanthipeptide-class-iii	55,153	77,750

Compact view

NMRK01000014.1 - Region 1 - NRPS

Download region GenBank file

Download region SVG

Location: 1 - 29,916 nt. (total: 29,916 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

No matches found.

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 29916:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg5_18

Search NORINE for peptide: strict or relaxed

AMP-binding (199..589): X

NRPSPredictor2: gly, ala, val, leu, ile, abu, iva

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: gmvlLaVVvK
Nearest Stachelhaus code:: gln
Stachelhaus code match:: 40% (weak)

AMP-binding (1249..1647): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: cys
Small clusters prediction:: cys
Single AA prediction:: cys

Stachelhaus prediction details:

Stachelhaus sequence:: DmFaVAcnwK
Nearest Stachelhaus code:: phe
Stachelhaus code match:: 50% (weak)

NMRK01000017.1 - Region 1 - NRPS

Download region GenBank file

Download region SVG

Location: 1 - 9,869 nt. (total: 9,869 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 9869:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (val - asp)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg8_1

Search NORINE for peptide: strict or relaxed

AMP-binding (268..662): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: val

Stachelhaus prediction details:

Stachelhaus sequence:: DAFWIGGTFK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 100% (strong)

AMP-binding (1300..1702): asp

NRPSPredictor2: asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asp

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKVGHIGK
Nearest Stachelhaus code:: asp
Stachelhaus code match:: 100% (strong)

NMRK01000001.1 - Region 1 - NRPS,NRPS-like,T3PKS,transAT-PKS

Download region GenBank file

Download region SVG

Location: 36,507 - 137,267 nt. (total: 100,761 nt) Show pHMM detection rules used

transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))
NRPS: cds(Condensation and (AMP-binding or A-OX))
T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 36506 - 137267:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (gly) + (ccmal - ohmal - gly) + (mal) + (ccmal - mal - ohmal - mal - mal) + (ccmal - mal - mal) + (mal) + (X - ccmal - ccmal) + (mal) + (mal - mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg11_54

Search NORINE for peptide: strict or relaxed

AMP-binding (195..599): X

NRPSPredictor2: hydrophobic-aliphatic

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVsnmAiIYK
Nearest Stachelhaus code:: ala
Stachelhaus code match:: 60% (weak)

ctg11_61

Search NORINE for peptide: strict or relaxed

AMP-binding (879..1187): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGMIWK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 100% (strong)

ctg11_62

Search NORINE for peptide: strict or relaxed

AMP-binding (49..245): X

NRPSPredictor2: hydrophobic-aliphatic

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: ---------K
Nearest Stachelhaus code:: val
Stachelhaus code match:: 10% (weak)

ctg11_67

PKS_AT (4..282): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 66.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 99.9
inactive: 30.9
Methoxymalonyl-CoA: 28.6
Methylmalonyl-CoA: 15.3
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ctg11_68

PKS_AT (7..293): mal

ATSignature: (unknown)

No matches above 50%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 29.5
inactive: 17.6
Methoxymalonyl-CoA: 11.9
Benzoyl-CoA: 9.4
Isobutyryl-CoA: 8.6
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ctg11_69

PKS_AT (4..285): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 98.2
inactive: 43.3
Methoxymalonyl-CoA: 36.6
Methylmalonyl-CoA: 24.6
Propionyl-CoA: 24.5
2-Methylbutyryl-CoA: 5.7
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

NMRK01000001.1 - Region 2 - terpene

Download region GenBank file

Download region SVG

Location: 699,510 - 720,253 nt. (total: 20,744 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

KnownClusterBlast

Similar known gene clusters

No matches found.

NMRK01000001.1 - Region 3 - PKS-like

Download region GenBank file

Download region SVG

Location: 804,950 - 846,194 nt. (total: 41,245 nt) Show pHMM detection rules used

PKS-like: (t2pks2 or ksIII)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NMRK01000021.1 - Region 1 - NRPS-like

Download region GenBank file

Download region SVG

Location: 1 - 1,151 nt. (total: 1,151 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NMRK01000002.1 - Region 1 - other

Download region GenBank file

Download region SVG

Location: 193,511 - 234,929 nt. (total: 41,419 nt) Show pHMM detection rules used

other: (LmbU or Neocarzinostat or fom1 or bcpB or frbD or mitE or vlmB or prnB or CaiA or bacilysin or orf2_PTase)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

KnownClusterBlast

Similar known gene clusters

NMRK01000002.1 - Region 2 - NRPS,RiPP-like

Download region GenBank file

Download region SVG

Location: 787,771 - 837,808 nt. (total: 50,038 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193)
NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 2, location 787770 - 837808:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (gly) + (X) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 792379 - 837808:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (gly) + (X) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg14_874

Search NORINE for peptide: strict or relaxed

AMP-binding (31..435): dhb

NRPSPredictor2: dhb

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: dhb, sal
Small clusters prediction:: dhb, sal
Single AA prediction:: dhb

Stachelhaus prediction details:

Stachelhaus sequence:: PLPAQGVVNK
Nearest Stachelhaus code:: dhb
Stachelhaus code match:: 100% (strong)

ctg14_876

Search NORINE for peptide: strict or relaxed

AMP-binding (456..855): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGLIWK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 100% (strong)

ctg14_877

Search NORINE for peptide: strict or relaxed

AMP-binding (389..669): X

NRPSPredictor2: hydrophobic-aliphatic

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DFWN-----K
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 50% (weak)

NMRK01000003.1 - Region 1 - terpene

Download region GenBank file

Download region SVG

Location: 16,887 - 38,770 nt. (total: 21,884 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

KnownClusterBlast

Similar known gene clusters

No matches found.

NMRK01000003.1 - Region 2 - T3PKS

Download region GenBank file

Download region SVG

Location: 93,989 - 135,089 nt. (total: 41,101 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

KnownClusterBlast

Similar known gene clusters

No matches found.

NMRK01000006.1 - Region 1 - NRPS

Download region GenBank file

Download region SVG

Location: 1 - 28,208 nt. (total: 28,208 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 28208:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (glu) + (leu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg18_1

Search NORINE for peptide: strict or relaxed

AMP-binding (23..420): leu

NRPSPredictor2: leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: leu

Stachelhaus prediction details:

Stachelhaus sequence:: DAFMMGMVFK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 100% (strong)

ctg18_2

Search NORINE for peptide: strict or relaxed

AMP-binding (465..867): glu

NRPSPredictor2: glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: glu, gln
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAKDLGVVDK
Nearest Stachelhaus code:: glu
Stachelhaus code match:: 100% (strong)

NMRK01000007.1 - Region 1 - NRPS

Download region GenBank file

Download region SVG

Location: 1 - 25,538 nt. (total: 25,538 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 25538:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ile)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg19_2

Search NORINE for peptide: strict or relaxed

AMP-binding (469..867): ile

NRPSPredictor2: ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: ile

Stachelhaus prediction details:

Stachelhaus sequence:: DGFFLGVVFK
Nearest Stachelhaus code:: ile
Stachelhaus code match:: 100% (strong)

NMRK01000008.1 - Region 1 - NRPS,betalactone,transAT-PKS

Download region GenBank file

Download region SVG

Location: 32,598 - 142,854 nt. (total: 110,257 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))
transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))
betalactone: (HMGL-like and AMP-binding)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 32597 - 142854:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (glu - D-tyr) + (ile) + (mal) + (asn) + (D-tyr - D-asn - gln - pro) + (pro) + (D-asn - ser)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 32597 - 109680:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal) + (asn) + (D-tyr - D-asn - gln - pro) + (pro) + (D-asn - ser)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 91312 - 142854:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (glu - D-tyr) + (ile)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg20_65

Search NORINE for peptide: strict or relaxed

AMP-binding (268..663): asn

NRPSPredictor2: asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asn

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKIGEVGK
Nearest Stachelhaus code:: asn
Stachelhaus code match:: 100% (strong)

AMP-binding (1778..2185): ser

NRPSPredictor2: ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: ser
Single AA prediction:: ser

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHFSLIDK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 100% (strong)

ctg20_67

Search NORINE for peptide: strict or relaxed

AMP-binding (268..662): tyr

NRPSPredictor2: tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: phe, trp, phg, tyr, bht
Small clusters prediction:: tyr, bht
Single AA prediction:: tyr

Stachelhaus prediction details:

Stachelhaus sequence:: DALSVGEVVK
Nearest Stachelhaus code:: tyr
Stachelhaus code match:: 100% (strong)

AMP-binding (1778..2173): asn

NRPSPredictor2: asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asn

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKIGEVGK
Nearest Stachelhaus code:: asn
Stachelhaus code match:: 100% (strong)

AMP-binding (3287..3692): gln

NRPSPredictor2: gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: glu, gln
Single AA prediction:: gln

Stachelhaus prediction details:

Stachelhaus sequence:: DAQDLGVVDK
Nearest Stachelhaus code:: gln
Stachelhaus code match:: 100% (strong)

AMP-binding (4334..4730): pro

NRPSPredictor2: pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: pro, pip
Small clusters prediction:: pro
Single AA prediction:: pro

Stachelhaus prediction details:

Stachelhaus sequence:: DVQFIAHVVK
Nearest Stachelhaus code:: pro
Stachelhaus code match:: 100% (strong)

ctg20_68

Search NORINE for peptide: strict or relaxed

AMP-binding (604..999): asn

NRPSPredictor2: asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asn

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKIGEVGK
Nearest Stachelhaus code:: asn
Stachelhaus code match:: 100% (strong)

ctg20_69

CAL_domain (36..478): pk

minowa: fatty_acid

Prediction, score:

fatty_acid: 614.1
NH2: 18.8
shikimic_acid: 0.0
Acetyl-CoA: 0.0
AHBA: 0.0

ctg20_70

PKS_AT (6..291): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 62.5%; inactive: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 75.6
inactive: 33.5
Methoxymalonyl-CoA: 20.3
Propionyl-CoA: 13.0
Methylmalonyl-CoA: 11.3
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ctg20_89

Search NORINE for peptide: strict or relaxed

AMP-binding (65..333): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: N/A
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: gclhIAApvK
Nearest Stachelhaus code:: phe
Stachelhaus code match:: 40% (weak)

ctg20_94

Search NORINE for peptide: strict or relaxed

AMP-binding (474..867): ile

NRPSPredictor2: ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: ile

Stachelhaus prediction details:

Stachelhaus sequence:: DAFFYGITFK
Nearest Stachelhaus code:: ile
Stachelhaus code match:: 100% (strong)

ctg20_95

Search NORINE for peptide: strict or relaxed

AMP-binding (464..868): glu

NRPSPredictor2: glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: glu, gln
Single AA prediction:: glu

Stachelhaus prediction details:

Stachelhaus sequence:: DAWHFGGVDK
Nearest Stachelhaus code:: glu
Stachelhaus code match:: 100% (strong)

AMP-binding (1499..1891): tyr

NRPSPredictor2: tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: phe, trp, phg, tyr, bht
Small clusters prediction:: tyr, bht
Single AA prediction:: tyr

Stachelhaus prediction details:

Stachelhaus sequence:: DGTITAEVAK
Nearest Stachelhaus code:: tyr
Stachelhaus code match:: 100% (strong)

NMRK01000009.1 - Region 1 - lanthipeptide-class-iii

Download region GenBank file

Download region SVG

Location: 55,153 - 77,750 nt. (total: 22,598 nt) Show pHMM detection rules used

lanthipeptide-class-iii: (micKC and not PKS_KS)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Lanthipeptides

KnownClusterBlast

Lanthipeptide predictions

Lanthipeptide(s) for ctg21_60 - Putative Class III

ctg21_62 leader / core peptide: MKDVLELQQLNEGTEVEASKGKGG - LLDhbLIDhbDhbIVGDhaDhaDhbIDhaNNC
ctg21_63 leader / core peptide: MRDVLELQQLNEGTEVEASKGKGG - LLDhbLIDhbDhbVVGDhbDhaDhbIDhaNNCG
ctg21_64 leader / core peptide: MRDVLELQQLNEGTEVEASKGKGG - LLDhbLIDhbDhbVVGDhbDhaDhbIDhaNNC
ctg21_65 leader / core peptide: MRDVLELQQLNEGTEVEASKGKGG - LLDhbLIDhbDhbVVGDhbDhaDhbIDhaNNCG
allorf_068156_068287 leader / core peptide: VDEQMNEVLELQQLSKGSEVE - DhbAGWDhbLLDhbDhbLIGDhbDhaDhbIDhaNDhaCNK

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Similar known gene clusters

No matches found.

Lanthipeptides

Core peptide predictions

ctg21_62 - Class III

Cleavage pHMM score: 0.00

RODEO score: 0

Monoisotopic mass: 1723.0 Da

Molecular weight: 1724.0 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1742.0 Da N = 1; 1760.0 Da N = 2; 1778.1 Da N = 3; 1796.1 Da N = 4; 1814.1 Da N = 5; 1832.1 Da N = 6

ctg21_63 - Class III

Cleavage pHMM score: 0.00

RODEO score: 0

Monoisotopic mass: 1780.0 Da

Molecular weight: 1781.1 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1799.1 Da N = 1; 1817.1 Da N = 2; 1835.1 Da N = 3; 1853.1 Da N = 4; 1871.2 Da N = 5; 1889.2 Da N = 6

ctg21_64 - Class III

Cleavage pHMM score: 0.00

RODEO score: 0

Monoisotopic mass: 1723.0 Da

Molecular weight: 1724.0 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1742.0 Da N = 1; 1760.0 Da N = 2; 1778.1 Da N = 3; 1796.1 Da N = 4; 1814.1 Da N = 5; 1832.1 Da N = 6

ctg21_65 - Class III

Cleavage pHMM score: 0.00

RODEO score: 0

Monoisotopic mass: 1780.0 Da

Molecular weight: 1781.1 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1799.1 Da N = 1; 1817.1 Da N = 2; 1835.1 Da N = 3; 1853.1 Da N = 4; 1871.2 Da N = 5; 1889.2 Da N = 6

allorf_068156_068287 - Class III

Cleavage pHMM score: -1.30

RODEO score: 0

Monoisotopic mass: 2119.2 Da

Molecular weight: 2120.4 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2138.4 Da N = 1; 2156.4 Da N = 2; 2174.4 Da N = 3; 2192.5 Da N = 4; 2210.5 Da N = 5; 2228.5 Da N = 6; 2246.5 Da N = 7; 2264.5 Da N = 8