GCA_003231475.1 - 6 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

NSIW01000016.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 8.1	RiPP-like	13,291	29,632
Region 8.2	RiPP-like	123,757	134,053

NSIW01000018.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 10.1	NRPS-like,T1PKS,NRPS,lanthipeptide-class-ii	40,193	129,473	salivaricin 9	RiPP:Lanthipeptide	72%

NSIW01000003.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 18.1	T3PKS	117,028	149,721

NSIW01000004.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 19.1	RiPP-like	74,996	87,092

NSIW01000008.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 23.1	lassopeptide	31,011	55,227

Region	Type	From	To	Most similar known cluster	Similarity
Region 8.1	RiPP-like	13,291	29,632
Region 8.2	RiPP-like	123,757	134,053
Region 10.1	NRPS-like,T1PKS,NRPS,lanthipeptide-class-ii	40,193	129,473	salivaricin 9	RiPP:Lanthipeptide	72%
Region 18.1	T3PKS	117,028	149,721
Region 19.1	RiPP-like	74,996	87,092
Region 23.1	lassopeptide	31,011	55,227

Compact view

NSIW01000016.1 - Region 1 - RiPP-like

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Location: 13,291 - 29,632 nt. (total: 16,342 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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KnownClusterBlast

Similar known gene clusters

No matches found.

NSIW01000016.1 - Region 2 - RiPP-like

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Location: 123,757 - 134,053 nt. (total: 10,297 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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KnownClusterBlast

Similar known gene clusters

No matches found.

NSIW01000018.1 - Region 1 - NRPS,NRPS-like,T1PKS,lanthipeptide-class-ii

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Location: 40,193 - 129,473 nt. (total: 89,281 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))
lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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NRPS/PKS domains

Lanthipeptides

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Lanthipeptide predictions

Lanthipeptide(s) for ctg10_85 - Putative Class II

ctg10_86 leader / core peptide: MKSTNNQSIAEIAAVNSLQEVSMEELDQIIGA - GNGVVLDhbLDhbHECNLADhbWDhbKKLKCC

Lanthipeptide(s) for ctg10_96 - Putative Class II

ctg10_97 leader / core peptide: MKNSKDILNNAIEEVSEKELMEVAGG - KRGDhbGWFADhbIDhbDDCPNDhaVFVCC

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Similar known gene clusters

Lanthipeptides

NRPS/PKS products

NRPS/PKS monomers

Core peptide predictions

ctg10_86 - Class II

Cleavage pHMM score: 5.20

RODEO score: 0

Monoisotopic mass: 2559.3 Da

Molecular weight: 2561.0 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 2579.1 Da N = 1

ctg10_97 - Class II

Cleavage pHMM score: 4.40

RODEO score: 0

Monoisotopic mass: 2347.1 Da

Molecular weight: 2348.7 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 2366.7 Da N = 1

Predicted core structure(s)

For candidate cluster 1, location 40192 - 129473:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (asp) + (X) + (pk) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 40192 - 88394:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (asp) + (X) + (pk) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 48395 - 99057:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (asp) + (X) + (pk) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg10_59

Search NORINE for peptide: strict or relaxed

AMP-binding (12..378): X

NRPSPredictor2: val, leu, ile, abu, iva

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DlLFFGaivK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 60% (weak)

AMP-binding (990..1376): X

NRPSPredictor2: orn, lys, arg

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: orn, lys, arg
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVsnnGSIcK
Nearest Stachelhaus code:: orn
Stachelhaus code match:: 60% (weak)

ctg10_60

PKS_AT (504..788): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 62.5%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 35.6
Methylmalonyl-CoA: 30.7
Propionyl-CoA: 16.0
Ethylmalonyl-CoA: 15.1
CHC-CoA: 11.1
trans-1,2-CPDA: 8.8
inactive: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0
Malonyl-CoA: -0.3

ctg10_61

Search NORINE for peptide: strict or relaxed

AMP-binding (471..823): X

NRPSPredictor2: hydrophobic-aliphatic

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DiEsiGTpVK
Nearest Stachelhaus code:: lys-b
Stachelhaus code match:: 60% (weak)

ctg10_63

Search NORINE for peptide: strict or relaxed

AMP-binding (19..400): asn

NRPSPredictor2: asn

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asn

Stachelhaus prediction details:

Stachelhaus sequence:: DATKVGEVGK
Nearest Stachelhaus code:: asn
Stachelhaus code match:: 100% (strong)

ctg10_64

Search NORINE for peptide: strict or relaxed

AMP-binding (541..937): asp

NRPSPredictor2: asp

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asp

Stachelhaus prediction details:

Stachelhaus sequence:: DfTKmGHIGK
Nearest Stachelhaus code:: asp
Stachelhaus code match:: 80% (moderate)

NSIW01000003.1 - Region 1 - T3PKS

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Location: 117,028 - 149,721 nt. (total: 32,694 nt) Show pHMM detection rules used

Region on contig edge.

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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KnownClusterBlast

Similar known gene clusters

No matches found.

NSIW01000004.1 - Region 1 - RiPP-like

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Location: 74,996 - 87,092 nt. (total: 12,097 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

KnownClusterBlast

Similar known gene clusters

No matches found.

NSIW01000008.1 - Region 1 - lassopeptide

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Location: 31,011 - 55,227 nt. (total: 24,217 nt) Show pHMM detection rules used

lassopeptide: (PF13471 and Asn_synthase or micJ25 or mcjC)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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KnownClusterBlast

Lasso peptides

Similar known gene clusters

No matches found.

Lasso peptide predictions

Lassopeptide(s) for allorf_045707_045829 - Putative Class II

allorf_045707_045829 leader / core peptide / cleaved tail: MNYTKPVIEKISTFKSGTS - GLVAGRFRDIFGGRALFLIRI

Lassopeptide(s) for ctg23_46 - Putative Class II

ctg23_46 leader / core peptide / cleaved tail: MKYCKPTFEPIATFKKDTK - GLWTGNFRDVFGGRAIVRVRIEF

Legend:

Red: Putative macrolactam
Purple: Putative cleaved off residues

Lasso peptides

Core peptide predictions

ctg23_46 - Class II: Cleavage pHMM score: -8.80; RODEO score: 16; Monoisotopic mass: 2646.4 Da; Molecular weight: 2648.0 Da; Number of bridges: 0
allorf_045707_045829 - Class II: Cleavage pHMM score: -8.10; RODEO score: 18; Monoisotopic mass: 2328.4 Da; Molecular weight: 2329.8 Da; Number of bridges: 0