LOCUS       RJWE01000103.1          3735 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  RJWE01000103.1 Limosilactobacillus reuteri strain PNW1
            NODE_104_length_3735_cov_616.956763, whole genome shotgun sequence.
ACCESSION   RJWE01000103
VERSION     RJWE01000103.1
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.0.0
            Run date     :: 2022-06-20 07:10:26
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3735
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3735
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[143:2657](+)"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      144..2657
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
     cand_cluster    1..3735
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(LANC_like and DUF4135)"
                     /kind="single"
                     /product="lanthipeptide-class-ii"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..3735
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-ii"
                     /region_number="1"
                     /rules="cds(LANC_like and DUF4135)"
                     /tool="antismash"
     CDS             144..2657
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070:lanthionine synthetase C family protein (Score:
                     550; E-value: 1.8e-166)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg5_1"
                     /sec_met_domain="LANC_like (E-value: 5.5e-29, bitscore:
                     89.1, seeds: 47, tool: rule-based-clusters)"
                     /sec_met_domain="DUF4135 (E-value: 1.6e-87, bitscore:
                     282.1, seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VLALELYKYKKEKIKSGIRISQADTDVLSSFIKENFCNETRFKAF
                     LRKYPTLSRIIITRVNFFVTNLSKLGEAIGTHRNDLCNFLHLKQENIVLTNIEIGVGDS
                     HCKGNTVIILELNHKKIVYKPRDLRILQQFEKFCNWINKSSSTLLDIDFLPKGLFYKDY
                     AFFEFVTRVPVKSKDECADYYERYGYLIVISYLLGLNDLHLENIIAKKDQPILVDGETM
                     FQNSLITPQNAFGKYLMSLNRDSIKGSCLLPTKLPMGNNEAIELSGLEGRETLLKNKVL
                     VPVNIENSNFHFEMRRQGYVKGGENIPITCNNKEVNVKKYRLAILDGYNKMTSFVSNNI
                     KELKKQVKEFSNFRIRILTKSTERYETMLNYAAHPSYNVEMKYRERLMLNIAAYPYDDK
                     RIVNSEVEDLLFGDVPYFSAVVGKTSITDSRGKNYDAYFKKSGLNLALDRINNFNYETI
                     EKERGLLLASLSVGDRITDVRKKSRDLLDQEFNYVRQASSIADTIMNSAFEDENSVIIS
                     TININNAGNWDILPMDESLYCGISGVTVFFIQLGYEKNEARYKEFAKKLLKASINMSLD
                     LEVQGAFKGFLSPLYPLLFHYSLYKTLDYEEVAYLKQLEKKIKQNIEKGELISDFINGL
                     GGIIKLLHLQKRLFGFKYISQNVMNRLEKKFMNCIMHSGKFGVAHGNCGDAMCLYEIGQ
                     LDQASALMKKITPVKNDMGWCKGVSGMLLARNYMQLDNNYAQEKLNDLENAPRNDTLCH
                     GKAGIIMIMHELFMQTGSTKWKEKANLLTNIMLGNYILNKDYSIRRFGKFSALGLFDGL
                     SGIGLSLLCHKGLKTNPLLLEIERG"
     CDS             2663..2848
                     /locus_tag="ctg5_2"
                     /transl_table=11
                     /translation="MEEKELEGVIGNSFESMTVEEMTKIQGMGEYQVDSTPGYFMESAA
                     FSALTANITRHAMHHH"
     CDS             2864..3094
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg5_3"
                     /transl_table=11
                     /translation="MDKEKLEKIVGNNFEEMSLQKMTEIQGMGEYQVDSTPAASAISRA
                     TIQVSRASSGKCLSWGSGAAFSAYFTHKRWC"
     CDS_motif       2864..2947
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MDKEKLEKIVGNNFEEMSLQKMTEIQGM"
                     /locus_tag="ctg5_3"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       2948..3094
                     /prepeptide="core"
                     /locus_tag="ctg5_3"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-2.10"
                     /molecular_weight="4882.4"
                     /monoisotopic_mass="4879.4"
                     /core_sequence="GEYQVDSTPAASAISRATIQVSRASSGKCLSWGSGAAFSAYFT
                     HKRWC"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MDKEKLEKIVGNNFEEMSLQKMTEIQGM"
                     /leader_location="[2863:2947](+)"
                     /alternative_weights="4900.4"
                     /alternative_weights="4918.4"
                     /alternative_weights="4936.4"
                     /alternative_weights="4954.4"
                     /alternative_weights="4972.5"
                     /alternative_weights="4990.5"
                     /alternative_weights="5008.5"
                     /alternative_weights="5026.5"
                     /alternative_weights="5044.5"
                     /alternative_weights="5062.6"
     CDS             3153..3734
                     /locus_tag="ctg5_4"
                     /transl_table=11
                     /translation="MRPVKNFPSLFFERMMNLKRILVLNGSCRYQGSTQFFLESLFNNL
                     DSKEYKVQYLFCRDFNKMRLNPNTLYDISYTQRNGDSKIYELEEMILKSDCFVVASPVY
                     MHNISSDLKLILEKLSTWSHTLRLDGKPCLVLSTCNSNGNTTVTGYLCRLCHNKQLINS
                     HFYRGVSELPYKLLFAVIYTHLEFRYEVSHT"
ORIGIN
        1 cttggctaat agtcgtaacc tctgcttgaa ggttcgttca gaatcggtag ggtaagtatg
       61 cgatgtttcc agaatctccc acttcaagca ttaaaccgta ggtttagcta agtgggagta
      121 gttcaattag acaaagtggg aaagtgctag cactagagtt atataaatat aaaaaagaga
      181 aaataaaatc tggtataaga attagtcaag ctgatacaga tgtactatcg tcttttataa
      241 aagaaaactt ttgtaatgaa actagattta aagcgttttt aagaaagtat ccaaccttat
      301 ctagaataat aataacgcgc gtcaattttt ttgtgacaaa tttatcaaaa cttggtgagg
      361 caattggcac ccatagaaat gatctttgta atttcttaca tcttaaacaa gaaaacattg
      421 tcttaactaa tatagaaatt ggagtgggag attcccattg caaaggaaat acagttataa
      481 tcttagaatt aaatcataaa aaaatagtat ataaacctcg tgatcttaga attcttcagc
      541 aatttgaaaa gttttgtaat tggattaata agtctagttc aactttactt gatatagatt
      601 ttttacctaa aggattattt tataaagatt atgctttttt tgaatttgta acacgggttc
      661 ctgtaaaaag taaagacgaa tgtgccgatt attatgaacg ttatggatac cttattgtta
      721 tttcttattt attagggcta aacgatttgc atttagagaa tataattgca aaaaaggacc
      781 aaccaattct tgttgatgga gaaacgatgt ttcaaaattc attaattact cctcaaaacg
      841 cgtttggaaa atatttaatg agtttgaata gagattcgat aaagggttcc tgcttattac
      901 ccacgaaatt accaatggga aataatgaag cgatagaact aagtggacta gaaggaagag
      961 aaactttatt gaaaaataaa gtactcgtac ccgtaaatat tgaaaattca aattttcatt
     1021 ttgaaatgag aagacaagga tacgttaaag gcggggaaaa tattccaatt acctgtaata
     1081 ataaagaagt taatgtaaaa aaatatagat tagcgattct agatggttat aacaaaatga
     1141 caagttttgt tagtaataat attaaagagt taaaaaaaca agttaaagaa ttttcaaact
     1201 ttagaattcg tattttaacg aagagtacag aacgttatga aacgatgtta aattatgcag
     1261 cacacccatc ttataatgtt gaaatgaaat atcgtgaaag gctaatgtta aatattgctg
     1321 cttatccata tgatgataag agaatagtaa atagtgaagt tgaagattta ctgtttgggg
     1381 atgttcccta tttttctgct gtagttggga agacaagtat tactgatagt agaggaaaaa
     1441 attatgatgc atattttaag aaatcaggac ttaaccttgc attagataga attaataatt
     1501 ttaattacga aacgatagaa aaagaaagag gacttctatt agcttcttta tcagtaggag
     1561 atagaatcac tgatgtaaga aagaaaagtc gagatttgtt agatcaggaa tttaattatg
     1621 tgagacaagc aagcagtatt gcggatacta ttatgaatag tgcattcgaa gatgaaaata
     1681 gtgttattat ttcgaccata aatatcaata atgcaggcaa ttgggatatt ttacctatgg
     1741 atgaaagtct atattgtgga attagtggtg taacagtatt ctttatacaa ttaggatatg
     1801 aaaaaaacga agctagatat aaagaatttg cgaagaaact attaaaagca tccattaata
     1861 tgtcattaga tctggaggta caaggagcct ttaagggatt tttatcgcca ctatacccat
     1921 tattatttca ttatagtctt tataaaacgt tggattatga agaagtggct tatttaaaac
     1981 agcttgaaaa gaagattaag caaaacattg aaaaaggcga actaattagt gattttatta
     2041 atggtttagg tggaattata aaacttttac atttacagaa aagattattt ggatttaaat
     2101 acatctcaca aaatgtaatg aatagattag aaaaaaagtt tatgaattgt attatgcatt
     2161 caggtaaatt cggtgttgcg catggtaatt gtggagacgc aatgtgctta tatgaaattg
     2221 ggcagctaga ccaagcaagt gctttaatga aaaaaattac tcctgtgaag aatgatatgg
     2281 gatggtgtaa aggagtatct ggaatgcttt tagcgcgtaa ttatatgcaa cttgataata
     2341 attacgcaca agaaaaatta aatgatttag aaaatgcgcc aaggaatgat acattatgcc
     2401 acggaaaagc aggaataatt atgattatgc atgaactttt tatgcagacg ggttctacta
     2461 aatggaagga aaaggccaac ttactaacta atattatgct aggaaattac atattaaata
     2521 aggactattc tattagaaga tttggaaagt tttctgcgtt aggcttattt gatggacttt
     2581 cgggtatcgg tttatcctta ttatgtcata aagggcttaa aactaatcca ttacttttgg
     2641 aaattgaaag ggggtgaaaa ttatggaaga aaaagaatta gaaggtgtaa tagggaattc
     2701 gtttgaaagt atgactgtag aggaaatgac aaaaattcaa ggtatgggtg aatatcaagt
     2761 agattcgacg cctggatatt ttatggaaag tgctgccttt tcagctctta cagccaatat
     2821 aacaagacat gctatgcatc atcattaaag ggaggtattt gttatggaca aagaaaaatt
     2881 agaaaaaatt gtaggtaata actttgagga aatgagttta caaaaaatga cagaaattca
     2941 aggcatgggt gaataccaag tggattcaac accagcagct tctgcgattt cacgggcaac
     3001 aattcaagta tcacgtgcat cttctggaaa atgtctaagt tggggtagtg gtgcagcatt
     3061 tagtgcttat tttactcata aaagatggtg ctagagtaat taaccgcctt gtggttaatc
     3121 cctatgatat cttaacaagg ttgatataaa gaatgaggcc ggtgaaaaat tttcccagcc
     3181 tcttttttga aaggatgatg aatttgaaac ggatattagt actgaacggc agttgtagat
     3241 atcaagggtc gacgcaattc tttttagaaa gcctctttaa caatctagat agcaaagagt
     3301 ataaagttca atatttgttt tgtagagact ttaacaagat gagactcaac ccgaatacgt
     3361 tatatgatat tagctatact caaagaaatg gtgattccaa aatttacgag ttagaagaaa
     3421 tgatattgaa atcagactgt tttgtagttg cttctccagt atatatgcat aatatttcgt
     3481 cagatttaaa attaatatta gaaaagcttt ctacttggtc acatacctta cgcttagatg
     3541 gtaaaccgtg tttagtttta agtacttgta attcaaatgg aaatactaca gtaacaggtt
     3601 atttgtgtag gctatgtcac aacaaacagt tgataaatag ccatttttat aggggagtat
     3661 cagaactccc ctataaactg ttatttgcag ttatctatac tcatttggaa tttcgatatg
     3721 aagtgtctca cacaa
//
