GCA_010578165.1 - 17 region(s)

Identified secondary metabolite regions using strictness 'relaxed'

NJRE01000001.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 1.1	terpene	199,769	220,572
Region 1.2	transAT-PKS,PKS-like,T3PKS,NRPS	807,482	914,557	bacillaene	Polyketide + NRP	100%

NJRE01000010.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 2.1	NRPS	192,081	218,504	surfactin	NRP:Lipopeptide	43%

NJRE01000015.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 7.1	NRPS	1	9,426	plipastatin	NRP	15%

NJRE01000016.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 8.1	NRPS	1	10,219	surfactin	NRP:Lipopeptide	8%

NJRE01000018.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 10.1	NRPS	1	15,131	plipastatin	NRP	30%

NJRE01000002.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 11.1	CDPS	267,220	287,966
Region 11.2	sactipeptide	513,314	534,925	subtilosin A	RiPP:Thiopeptide	100%
Region 11.3	other	541,713	583,131	bacilysin	Other	100%
Region 11.4	epipeptide	800,256	821,954	thailanstatin A	NRP + Polyketide	10%

NJRE01000003.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 12.1	NRPS,betalactone	37,355	65,399	fengycin	NRP	80%

NJRE01000005.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 14.1	T3PKS	816,755	857,852
Region 14.2	terpene	905,594	927,492
Region 14.3	NRPS	989,110	1,011,869	plipastatin	NRP	38%

NJRE01000007.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 16.1	NRPS	1	27,659	surfactin	NRP:Lipopeptide	43%

NJRE01000008.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 17.1	NRPS	80,789	127,925	bacillibactin	NRP	100%
Region 17.2	lanthipeptide-class-i,lanthipeptide-class-ii	276,766	326,507	subtilin	RiPP:Lanthipeptide	100%

Region	Type	From	To	Most similar known cluster	Similarity
Region 1.1	terpene	199,769	220,572
Region 1.2	transAT-PKS,PKS-like,T3PKS,NRPS	807,482	914,557	bacillaene	Polyketide + NRP	100%
Region 2.1	NRPS	192,081	218,504	surfactin	NRP:Lipopeptide	43%
Region 7.1	NRPS	1	9,426	plipastatin	NRP	15%
Region 8.1	NRPS	1	10,219	surfactin	NRP:Lipopeptide	8%
Region 10.1	NRPS	1	15,131	plipastatin	NRP	30%
Region 11.1	CDPS	267,220	287,966
Region 11.2	sactipeptide	513,314	534,925	subtilosin A	RiPP:Thiopeptide	100%
Region 11.3	other	541,713	583,131	bacilysin	Other	100%
Region 11.4	epipeptide	800,256	821,954	thailanstatin A	NRP + Polyketide	10%
Region 12.1	NRPS,betalactone	37,355	65,399	fengycin	NRP	80%
Region 14.1	T3PKS	816,755	857,852
Region 14.2	terpene	905,594	927,492
Region 14.3	NRPS	989,110	1,011,869	plipastatin	NRP	38%
Region 16.1	NRPS	1	27,659	surfactin	NRP:Lipopeptide	43%
Region 17.1	NRPS	80,789	127,925	bacillibactin	NRP	100%
Region 17.2	lanthipeptide-class-i,lanthipeptide-class-ii	276,766	326,507	subtilin	RiPP:Lanthipeptide	100%

No secondary metabolite regions were found in these records:: NJRE01000011.1; NJRE01000012.1; NJRE01000013.1; NJRE01000014.1; NJRE01000017.1; NJRE01000004.1; NJRE01000006.1; NJRE01000009.1

Compact view

NJRE01000001.1 - Region 1 - terpene

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Location: 199,769 - 220,572 nt. (total: 20,804 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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KnownClusterBlast

Similar known gene clusters

No matches found.

NJRE01000001.1 - Region 2 - NRPS,PKS-like,T3PKS,transAT-PKS

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Location: 807,482 - 914,557 nt. (total: 107,076 nt) Show pHMM detection rules used

transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))
PKS-like: (t2pks2 or ksIII)
T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)
NRPS: cds(Condensation and (AMP-binding or A-OX))

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 2, location 807481 - 914557:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (gly - ccmal - ohmal - mal) + (mal - ccmal - mal - ohmal - mal) + (mal - ccmal - ohmal - mal) + (X - ccmal - ccmal - ccmal) + (ccmal - mal - mal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 807481 - 898026:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (gly - ccmal - ohmal - mal) + (mal - ccmal - mal - ohmal - mal) + (mal - ccmal - ohmal - mal) + (X - ccmal - ccmal - ccmal) + (ccmal - mal - mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg1_849

PKS_AT (4..284): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 98.0
Methoxymalonyl-CoA: 52.0
inactive: 34.2
Methylmalonyl-CoA: 33.8
Propionyl-CoA: 22.5
Ethylmalonyl-CoA: 10.8
2-Methylbutyryl-CoA: 9.4
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

ctg1_850

PKS_AT (7..293): pk

ATSignature: (unknown)

No matches above 50%

minowa: inactive

Prediction, score:

inactive: 27.3
Malonyl-CoA: 24.5
Isobutyryl-CoA: 8.5
Benzoyl-CoA: 8.4
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ctg1_851

PKS_AT (4..288): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 66.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 96.2
inactive: 40.7
Methoxymalonyl-CoA: 29.2
Methylmalonyl-CoA: 22.2
Propionyl-CoA: 16.2
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ctg1_857

Search NORINE for peptide: strict or relaxed

CAL_domain (63..471): pk

minowa: fatty_acid

Prediction, score:

fatty_acid: 16.1
NH2: 10.3
shikimic_acid: 0.0
Acetyl-CoA: 0.0
AHBA: 0.0

AMP-binding (1159..1554): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGMIWK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 100% (strong)

ctg1_860

Search NORINE for peptide: strict or relaxed

AMP-binding (497..905): X

NRPSPredictor2: hydrophobic-aliphatic

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVsnmAiIYK
Nearest Stachelhaus code:: ala
Stachelhaus code match:: 60% (weak)

NJRE01000010.1 - Region 1 - NRPS

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Location: 192,081 - 218,504 nt. (total: 26,424 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

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Gene details

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 192080 - 218504:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (leu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg2_229

Search NORINE for peptide: strict or relaxed

AMP-binding (469..867): leu

NRPSPredictor2: leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: leu

Stachelhaus prediction details:

Stachelhaus sequence:: DAFFLGCVFK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 100% (strong)

NJRE01000015.1 - Region 1 - NRPS

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Location: 1 - 9,426 nt. (total: 9,426 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 9426:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-val) + (pro)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg7_1

Search NORINE for peptide: strict or relaxed

AMP-binding (468..865): pro

NRPSPredictor2: pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: pro, pip
Small clusters prediction:: pro
Single AA prediction:: pro

Stachelhaus prediction details:

Stachelhaus sequence:: DVQFIAHVVK
Nearest Stachelhaus code:: pro
Stachelhaus code match:: 100% (strong)

AMP-binding (1499..1900): glu

NRPSPredictor2: glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: glu, gln
Single AA prediction:: glu

Stachelhaus prediction details:

Stachelhaus sequence:: DAWHFGSVEK
Nearest Stachelhaus code:: glu
Stachelhaus code match:: 100% (strong)

ctg7_2

Search NORINE for peptide: strict or relaxed

AMP-binding (132..525): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: val

Stachelhaus prediction details:

Stachelhaus sequence:: DVFWIGGTFK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 100% (strong)

NJRE01000016.1 - Region 1 - NRPS

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Location: 1 - 10,219 nt. (total: 10,219 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 10219:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (val - asp)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg8_1

Search NORINE for peptide: strict or relaxed

AMP-binding (470..864): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: val

Stachelhaus prediction details:

Stachelhaus sequence:: DAFWIGGTFK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 100% (strong)

AMP-binding (1501..1904): asp

NRPSPredictor2: asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asp

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKVGHIGK
Nearest Stachelhaus code:: asp
Stachelhaus code match:: 100% (strong)

NJRE01000018.1 - Region 1 - NRPS

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Location: 1 - 15,131 nt. (total: 15,131 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 15131:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-orn) + (tyr - D-thr) + (glu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg10_1

Search NORINE for peptide: strict or relaxed

AMP-binding (469..870): glu

NRPSPredictor2: glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: glu, gln
Single AA prediction:: glu

Stachelhaus prediction details:

Stachelhaus sequence:: DAWHFGGVDK
Nearest Stachelhaus code:: glu
Stachelhaus code match:: 100% (strong)

ctg10_2

Search NORINE for peptide: strict or relaxed

AMP-binding (474..865): tyr

NRPSPredictor2: tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: phe, trp, phg, tyr, bht
Small clusters prediction:: tyr, bht
Single AA prediction:: tyr

Stachelhaus prediction details:

Stachelhaus sequence:: DGTITAEVAK
Nearest Stachelhaus code:: tyr
Stachelhaus code match:: 100% (strong)

AMP-binding (1505..1903): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

ctg10_3

Search NORINE for peptide: strict or relaxed

AMP-binding (129..534): orn

NRPSPredictor2: orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: orn, lys, arg
Small clusters prediction:: orn, horn
Single AA prediction:: orn

Stachelhaus prediction details:

Stachelhaus sequence:: DVGEIGSIDK
Nearest Stachelhaus code:: orn
Stachelhaus code match:: 100% (strong)

NJRE01000002.1 - Region 1 - CDPS

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Location: 267,220 - 287,966 nt. (total: 20,747 nt) Show pHMM detection rules used

CDPS: (CDPS or cycdipepsynth)

Legend:

core biosynthetic genes

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KnownClusterBlast

Similar known gene clusters

No matches found.

NJRE01000002.1 - Region 2 - sactipeptide

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Location: 513,314 - 534,925 nt. (total: 21,612 nt) Show pHMM detection rules used

sactipeptide: (subtilosin or thuricin or TIGR04404 or Subtilosin_A or skfc)

Legend:

core biosynthetic genes

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KnownClusterBlast

Sactipeptides

Similar known gene clusters

Sactipeptide predictions

Putative Sactipeptide(s)

ctg11_508 leader / core peptide: MKKAVIVENK - GCATCSIGAACLVDGPIPDFEIAGATGLFGLWG

Sactipeptides

Core peptide predictions

ctg11_508: Putative sactipeptide; RODEO score: 33.0; Core sequence, approximate: GCATCSIGAACLVDGPIPDFEIAGATGLFGLWG

NJRE01000002.1 - Region 3 - other

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Location: 541,713 - 583,131 nt. (total: 41,419 nt) Show pHMM detection rules used

other: (LmbU or Neocarzinostat or fom1 or bcpB or frbD or mitE or vlmB or prnB or CaiA or bacilysin or orf2_PTase)

Legend:

core biosynthetic genes

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KnownClusterBlast

Similar known gene clusters

NJRE01000002.1 - Region 4 - epipeptide

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Location: 800,256 - 821,954 nt. (total: 21,699 nt) Show pHMM detection rules used

epipeptide: (TIGR04078 and yydH)

Legend:

core biosynthetic genes

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KnownClusterBlast

Similar known gene clusters

NJRE01000003.1 - Region 1 - NRPS,betalactone

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Location: 37,355 - 65,399 nt. (total: 28,045 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
betalactone: (HMGL-like and AMP-binding)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 37354 - 65399:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-tyr) + (ile)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg12_64

Search NORINE for peptide: strict or relaxed

AMP-binding (13..445): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: N/A
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: gclhIAApvK
Nearest Stachelhaus code:: phe
Stachelhaus code match:: 40% (weak)

ctg12_68

Search NORINE for peptide: strict or relaxed

AMP-binding (474..867): ile

NRPSPredictor2: ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: ile

Stachelhaus prediction details:

Stachelhaus sequence:: DAFFYGITFK
Nearest Stachelhaus code:: ile
Stachelhaus code match:: 100% (strong)

ctg12_69

Search NORINE for peptide: strict or relaxed

AMP-binding (333..727): tyr

NRPSPredictor2: tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: phe, trp, phg, tyr, bht
Small clusters prediction:: tyr, bht
Single AA prediction:: tyr

Stachelhaus prediction details:

Stachelhaus sequence:: DGTITAEVAK
Nearest Stachelhaus code:: tyr
Stachelhaus code match:: 100% (strong)

NJRE01000005.1 - Region 1 - T3PKS

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Location: 816,755 - 857,852 nt. (total: 41,098 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

Legend:

core biosynthetic genes

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Gene details

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KnownClusterBlast

Similar known gene clusters

No matches found.

NJRE01000005.1 - Region 2 - terpene

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Location: 905,594 - 927,492 nt. (total: 21,899 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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KnownClusterBlast

Similar known gene clusters

No matches found.

NJRE01000005.1 - Region 3 - NRPS

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Location: 989,110 - 1,011,869 nt. (total: 22,760 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NJRE01000007.1 - Region 1 - NRPS

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Location: 1 - 27,659 nt. (total: 27,659 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 27659:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (glu - leu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg16_1

Search NORINE for peptide: strict or relaxed

AMP-binding (466..867): glu

NRPSPredictor2: glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: glu, gln
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAKDLGVVDK
Nearest Stachelhaus code:: glu
Stachelhaus code match:: 100% (strong)

AMP-binding (1510..1908): leu

NRPSPredictor2: leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: leu

Stachelhaus prediction details:

Stachelhaus sequence:: DAFMMGMVFK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 100% (strong)

NJRE01000008.1 - Region 1 - NRPS

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Location: 80,789 - 127,925 nt. (total: 47,137 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 80788 - 127925:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (gly - thr)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg17_99

Search NORINE for peptide: strict or relaxed

AMP-binding (463..861): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGLIWK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 100% (strong)

AMP-binding (1519..1935): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

ctg17_101

Search NORINE for peptide: strict or relaxed

AMP-binding (31..435): dhb

NRPSPredictor2: dhb

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: dhb, sal
Small clusters prediction:: dhb, sal
Single AA prediction:: dhb

Stachelhaus prediction details:

Stachelhaus sequence:: PLPAQGVVNK
Nearest Stachelhaus code:: dhb
Stachelhaus code match:: 100% (strong)

NJRE01000008.1 - Region 2 - lanthipeptide-class-i,lanthipeptide-class-ii

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Location: 276,766 - 326,507 nt. (total: 49,742 nt) Show pHMM detection rules used

lanthipeptide-class-i: ((LANC_like and (Lant_dehydr_N or Lant_dehydr_C)) and not (YcaO or TIGR03882))
lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Lanthipeptides

KnownClusterBlast

Lanthipeptide predictions

Lanthipeptide(s) for ctg17_287 - Putative Class I

ctg17_284 leader / core peptide: MSKFDDFDLDVVKVSKQDSKITPQ - WKDhaEDhaLCDhbPGCVDhbGVLQDhbCFLQDhbIDhbCNCKIDhaK

Lanthipeptide(s) for ctg17_306 - Putative Class II

ctg17_307 leader / core peptide: MKKDMVKNASGFIEEDELISLANGENATGG - GDhbPVDhbAIIDhbALDhbGDhbDhbFDhbVDhbLDhaAADhaCPDhbDhaACDhbNLCNK
ctg17_309 leader / core peptide: MKKTKILKTPELKVTKDLNLIDPCGDVLEEIKEHEVSGA - FNDhbWNDhbDhbDhbDhbDhaVGDhbILVDhaEVFGNKGKVCDhbGDhbKECQGDhbC

Lanthipeptide(s) for ctg17_308 - Putative Class II

ctg17_307 leader / core peptide: MKKDMVKNASGFIEEDELISLANGENATGG - GDhbPVDhbAIIDhbALDhbGDhbDhbFDhbVDhbLDhaAADhaCPDhbDhaACDhbNLCNK
ctg17_309 leader / core peptide: MKKTKILKTPELKVTKDLNLIDPCGDVLEEIKEHEVSGA - FNDhbWNDhbDhbDhbDhbDhaVGDhbILVDhaEVFGNKGKVCDhbGDhbKECQGDhbC
allorf_317533_317664 leader / core peptide: MAYMKMICPHDKGEPLYDMEPEVFGG - NLVDhbADhaDhaVDhaCLELRGKY
allorf_317633_317809 leader / core peptide: LEGILGVKSFLENGQQSFKTLRDGCHNLRVTVGS - ANNGCLEDhaRKHDhbDhaNNGIYENDLDhaDhb
allorf_317867_318043 leader / core peptide: LFLNTVETS - LDhaLRIDhaYNIRLDIFNGRDhbEHGKFQKRCDhaIFKDhaHDNRMNKVMIDhbDhbMEHCV

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Similar known gene clusters

Lanthipeptides

Core peptide predictions

ctg17_284 - Class I

Cleavage pHMM score: 27.80

RODEO score: 30

Monoisotopic mass: 3346.7 Da

Molecular weight: 3349.0 Da

Number of bridges: 5

Alternative weights: (assuming N unmodified Ser/Thr residues); 3367.0 Da N = 1; 3385.0 Da N = 2; 3403.0 Da N = 3

ctg17_307 - Class II

Cleavage pHMM score: -5.20

RODEO score: 0

Monoisotopic mass: 3294.8 Da

Molecular weight: 3296.8 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 3314.8 Da N = 1; 3332.8 Da N = 2; 3350.8 Da N = 3; 3368.9 Da N = 4; 3386.9 Da N = 5; 3404.9 Da N = 6; 3422.9 Da N = 7; 3440.9 Da N = 8; 3459.0 Da N = 9; 3477.0 Da N = 10

ctg17_309 - Class II

Cleavage pHMM score: -3.20

RODEO score: 0

Monoisotopic mass: 3712.9 Da

Molecular weight: 3715.1 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 3733.2 Da N = 1; 3751.2 Da N = 2; 3769.2 Da N = 3; 3787.2 Da N = 4; 3805.2 Da N = 5; 3823.3 Da N = 6; 3841.3 Da N = 7; 3859.3 Da N = 8

ctg17_307 - Class II

Cleavage pHMM score: -5.20

RODEO score: 0

Monoisotopic mass: 3294.8 Da

Molecular weight: 3296.8 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 3314.8 Da N = 1; 3332.8 Da N = 2; 3350.8 Da N = 3; 3368.9 Da N = 4; 3386.9 Da N = 5; 3404.9 Da N = 6; 3422.9 Da N = 7; 3440.9 Da N = 8; 3459.0 Da N = 9; 3477.0 Da N = 10

ctg17_309 - Class II

Cleavage pHMM score: -3.20

RODEO score: 0

Monoisotopic mass: 3712.9 Da

Molecular weight: 3715.1 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 3733.2 Da N = 1; 3751.2 Da N = 2; 3769.2 Da N = 3; 3787.2 Da N = 4; 3805.2 Da N = 5; 3823.3 Da N = 6; 3841.3 Da N = 7; 3859.3 Da N = 8

allorf_317533_317664 - Class II

Cleavage pHMM score: -5.70

RODEO score: 0

Monoisotopic mass: 1767.0 Da

Molecular weight: 1768.0 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1786.0 Da N = 1; 1804.1 Da N = 2; 1822.1 Da N = 3

allorf_317633_317809 - Class II

Cleavage pHMM score: -5.00

RODEO score: 0

Monoisotopic mass: 2546.2 Da

Molecular weight: 2547.7 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2565.7 Da N = 1; 2583.7 Da N = 2; 2601.7 Da N = 3; 2619.7 Da N = 4

allorf_317867_318043 - Class II

Cleavage pHMM score: -6.40

RODEO score: 0

Monoisotopic mass: 5740.0 Da

Molecular weight: 5743.7 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 5761.7 Da N = 1; 5779.7 Da N = 2; 5797.7 Da N = 3; 5815.7 Da N = 4; 5833.8 Da N = 5