LOCUS       c00004_JAAEBI0..        1977 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  JAAEBI010000013.1 Bacillus subtilis strain TNC3(2019)
            NODE_13_length_1977_cov_6.405405, whole genome shotgun sequence.
ACCESSION   JAAEBI010000013
VERSION     JAAEBI010000013
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.0.0
            Run date     :: 2022-06-20 23:01:56
            Original ID  :: JAAEBI010000013.1
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 1977
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..1977
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1:1975](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(2..1975)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     cand_cluster    1..1977
                     /SMILES="NC(CCC(=O)O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..1977
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX))"
                     /tool="antismash"
     CDS             complement(2..1975)
                     /NRPS_PKS="Domain: AMP-binding (0-335). E-value: 6e-89.
                     Score: 290.2. Matches aSDomain:
                     nrpspksdomains_ctg4_1_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (437-506). E-value: 4.6e-26. Score:
                     82.6. Matches aSDomain: nrpspksdomains_ctg4_1_PCP.1"
                     /NRPS_PKS="type: NRPS-like protein"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     295.8; E-value: 8e-90)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg4_1"
                     /sec_met_domain="Condensation (E-value: 4.9e-48, bitscore:
                     149.8, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 1.7e-107, bitscore:
                     345.6, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 4e-18, bitscore:
                     52.0, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="KAGGAYLPLDPAYPKERLSYMLKDSGASLLLTQPGCSAPNFSGET
                     LEVDMTSLASEKAENHLFTPADGGSLAYVIYTSGSTGQPKGVAVEHRQAVSFLTGMQHQ
                     FPLSEDDIVMVKTSFSFDASVWQLFWWALSGASAYLLPPGWEKDSALIVKAITQEKVTT
                     AHFIPAMLNSFLDQAEMLALGDGTNLKRVFAGGEPLAPRTAARFASVLPQVSLIHGYGP
                     TEATVDAAFYVLDPERDRDRLRIPIGKPVPGARLYVLDPHLAVQPSGVAGELYIAGAGV
                     ARGYLNRPALTEERFLEDPFYPGERMYKTGDVARWLPDGNIEFLGRLDDQVKIRGYRIE
                     PGEIEAALRSIEGVREAAVTVRTDSGEPELCAYVEGLQRNEVRAQLESLLPGYMVPAYM
                     IEMEQWPVTPSGKLDRNALPAPGGAADAETYTAPRNVTEMKLSQLWEDVLKNGPVGIHD
                     NFFDRGGHSLKATALVSRIAKEFDVQVPLKDVFAHPTVEGLATVIRKGTDSPYEAIKPA
                     EKQETYPVSSAQKRIYVLQQLEDGGTGYNMPAVLELEGKLNPERMDRAFKELIKRHESL
                     RTSFEQDAGGDPVQRIHDEVPFTLQTTVLGERTEEEAAAAFIKPFDLSQAPLFRAQIVK
                     VSDERHLLLVDMHHIISDGVSVN"
     CDS_motif       complement(236..343)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg4_1_0005"
                     /evalue="3.00E-17"
                     /label="C2_LCL_024-062"
                     /locus_tag="ctg4_1"
                     /protein_end="580"
                     /protein_start="544"
                     /score="55.0"
                     /tool="antismash"
                     /translation="YNMPAVLELEGKLNPERMDRAFKELIKRHESLRTSF"
     aSDomain        complement(458..664)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg4_1_PCP.1"
                     /evalue="4.60E-26"
                     /label="ctg4_1_PCP.1"
                     /locus_tag="ctg4_1"
                     /protein_end="506"
                     /protein_start="437"
                     /score="82.6"
                     /tool="antismash"
                     /translation="EMKLSQLWEDVLKNGPVGIHDNFFDRGGHSLKATALVSRIAKEFD
                     VQVPLKDVFAHPTVEGLATVIRKG"
     aSModule        458..1975
                     /complete
                     /domains="nrpspksdomains_ctg4_1_AMP-binding.1"
                     /domains="nrpspksdomains_ctg4_1_PCP.1"
                     /locus_tags="ctg4_1"
                     /monomer_pairings="glu -> glu"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(941..1006)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg4_1_0004"
                     /evalue="3.20E-13"
                     /label="NRPS-A_a8"
                     /locus_tag="ctg4_1"
                     /protein_end="345"
                     /protein_start="323"
                     /score="41.4"
                     /tool="antismash"
                     /translation="FLGRLDDQVKIRGYRIEPGEIE"
     aSDomain        complement(971..1975)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg4_1_AMP-binding.1"
                     /evalue="6.00E-89"
                     /label="ctg4_1_AMP-binding.1"
                     /locus_tag="ctg4_1"
                     /protein_end="335"
                     /protein_start="0"
                     /score="290.2"
                     /specificity="consensus: glu"
                     /tool="antismash"
                     /translation="KAGGAYLPLDPAYPKERLSYMLKDSGASLLLTQPGCSAPNFSGET
                     LEVDMTSLASEKAENHLFTPADGGSLAYVIYTSGSTGQPKGVAVEHRQAVSFLTGMQHQ
                     FPLSEDDIVMVKTSFSFDASVWQLFWWALSGASAYLLPPGWEKDSALIVKAITQEKVTT
                     AHFIPAMLNSFLDQAEMLALGDGTNLKRVFAGGEPLAPRTAARFASVLPQVSLIHGYGP
                     TEATVDAAFYVLDPERDRDRLRIPIGKPVPGARLYVLDPHLAVQPSGVAGELYIAGAGV
                     ARGYLNRPALTEERFLEDPFYPGERMYKTGDVARWLPDGNIEFLGRLDDQVKIR"
     CDS_motif       complement(1088..1177)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg4_1_0003"
                     /evalue="3.70E-18"
                     /label="NRPS-A_a6"
                     /locus_tag="ctg4_1"
                     /protein_end="296"
                     /protein_start="266"
                     /score="57.6"
                     /tool="antismash"
                     /translation="PSGVAGELYIAGAGVARGYLNRPALTEERF"
     CDS_motif       complement(1703..1762)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg4_1_0002"
                     /evalue="2.60E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="ctg4_1"
                     /protein_end="91"
                     /protein_start="71"
                     /score="35.2"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGQPKGVAVEH"
     CDS_motif       complement(1946..1975)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg4_1_0001"
                     /evalue="5.20E-03"
                     /label="NRPS-A_a2"
                     /locus_tag="ctg4_1"
                     /protein_end="10"
                     /protein_start="0"
                     /score="10.5"
                     /tool="antismash"
                     /translation="KAGGAYLPLD"
ORIGIN
        1 tgttgacgga aacaccgtct gaaatgatgt gatgcatatc caccagcaac aggtgccgct
       61 catctgatac ctttacgatt tgggcacgga acagcggcgc ttggctgaga tcaaacggtt
      121 tgataaatgc ggctgcggcc tcctcttcgg ttcgttcgcc gagaactgta gtctgtaacg
      181 taaacggcac ctcgtcatgg atgcgctgca ccggatcgcc tcctgcgtcc tgttcgaagg
      241 aggtccgaag cgactcgtgg cgcttgatca gctctttgaa agctctgtcc atcctttcag
      301 ggttgagctt cccttcgagc tctaacacag ctggcatatt gtagcctgtc cctccgtcct
      361 ccagctgctg aaggacataa atccgctttt gggcagagga aaccggatac gtttcctgtt
      421 tttcagcggg cttaatcgct tcatatggac tgtccgttcc tttacggata acggtagcca
      481 gtccttctac cgtcggatgg gcaaacacat cttttagcgg cacctgtaca tcgaattctt
      541 tggcaatccg tgagacaagc gccgttgctt ttaaggaatg tcccccgcgg tcaaaaaagt
      601 tgtcgtgaat cccgacaggg ccatttttca gcacgtcctc ccatagctga gaaagcttca
      661 tctcagtcac gtttctcggc gccgtgtagg tctccgcatc tgccgctccg ccaggagctg
      721 gcagggcgtt tcggtcgagc tttccgcttg gtgtaaccgg ccactgttcc atttctatca
      781 tataggctgg gaccatgtag cccggcaaca ggctctcaag ctgcgctcgg acttcgtttc
      841 tttggagccc ttctacataa gcgcataatt ccggctcgcc gctgtctgtc cgtacggtaa
      901 cggccgcctc tcttacacct tctatacttc ttagtgccgc ttcaatttct cccggttcaa
      961 tccggtagcc gcgaattttc acctgatcat ccaaccgtcc caggaattca atattcccgt
     1021 cagggagcca gcgcgccaca tctcctgttt tgtacatacg ttcacccggg taaaacgggt
     1081 cttctagaaa acgctcctcc gttaatgccg gccgattcaa ataacctctg gcaacacccg
     1141 ctccggcaat gtatagttcg ccggcgacac cggaaggctg tacggctaaa tgcggatcta
     1201 aaacatacaa acgcgcacca ggtacgggct tcccaatcgg aatccgcaaa cggtctctgt
     1261 cccgttctgg atccaatacg taaaatgccg cgtctaccgt tgcttctgtc ggcccgtagc
     1321 catggattaa tgatacctgc ggcaatacag aagcaaaacg ggctgccgta cgcggcgcaa
     1381 gcggttcacc tccggcgaat acacgcttta ggtttgtccc gtcaccaagc gctaacattt
     1441 ctgcttgatc cagaaagcta ttcagcatag ccggaataaa atgagccgtt gtcacttttt
     1501 cctgagtaat ggcttttacg atcaatgcgg agtctttttc ccagccgggc ggaagcagat
     1561 acgccgaagc accggaaaga gcccaccaaa acagctgcca cacagacgca tcaaaggaaa
     1621 aagaggtttt caccatgaca atgtcatctt ctgaaagcgg aaactggtgc tgcatgccgg
     1681 tcagaaagga aacagcctga cggtgctcaa cggccactcc tttcggctgc ccggtagagc
     1741 ccgatgtata gatgacatag gcgagagaac cgccgtcagc aggagtaaac agatggtttt
     1801 ctgctttttc gctcgcaaga gatgtcatgt ccacttcaag cgtctctccg gaaaagttcg
     1861 gtgcggagca tcccggctgt gtcagcaaga gcgatgcccc gctgtctttc agcatgtagc
     1921 tcagccgctc ctttggatac gcaggatcaa gcggcaaata cgcccctccg gccttca
//
