LOCUS       c00020_JAAJBN0..       13431 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  JAAJBN010000028.1 Ligilactobacillus salivarius strain VA40-10
            NODE_28_length_15149_cov_111.600719, whole genome shotgun sequence.
ACCESSION   JAAJBN010000028
VERSION     JAAJBN010000028
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2022-06-21 18:26:37
            Original ID  :: JAAJBN010000028.1
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 13431
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..13431
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[300:3431]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      301..3431
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..13431
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..13431
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(2..100)
                     /locus_tag="ctg20_1"
                     /transl_table=11
                     /translation="MKKFEIPLYYYHKKYRRIFYTMEETEIVEVKPT"
     CDS             301..2568
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg20_2"
                     /sec_met_domain="Gly_radical (E-value: 5.9e-41, bitscore:
                     129.7, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 7.4e-262, bitscore:
                     860.2, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVEKTQMQNVDVKDAWEGFRGKEWKEEIDIREFIQDNFTPYDGDQ
                     SFLEGPTEATTKLNDKLIDLKLKERAHGGPLEADTKVVSTITSHKPGYLDKSLEKIVGL
                     QTDKPMKRALMPYGGIRMAKGALEAYGFKIDPETDFVFENLSKTHNQGVFDAYTSEIMK
                     ARHNKIITGLPDAYGRGRLIGDFPRIALYGIDRLIEGKKKDLENCGDGEMTNDVIQMRE
                     EISDQIKALNDMKIMAESYGYDISKPATTAKEAIQWLYFGYLASIKQQNGAAMSIGRID
                     SFIDIYIERDMKRGILTEKEAQELIDQFTMKLRMVCFIRTPAYNSLFSGNPIWATLSIA
                     GMGLDGRHHVTKTSYRFLNTLHNMGAAPEPNITLLWSDRLPESFKHYAASVSIASSTIQ
                     YENDDLMMKTWGTDYYGIACCVSAQPIANGMQFFGARANLAKAVLYAINGGKDELTKQQ
                     VGPAYEPISSEYIDYDEFMKKFDDMMEWIADIYVNALNTIHYMHDKYYYESAQMALKDT
                     LVDRSFATGISGLSVAADSISAIKYGHVKVIRDEDGIAVDFKADHDFPRYGNDDDRVDD
                     IAAWLVKELFTKMSKHHLYRGAKLTTSVLTITSNVVYGKNTGTTPNGRQKGEPFAPGAN
                     PAYGADTTGALASLLSVAKLPYKYATDGISNTFAVTPTTLGHDEKMQEDTLVNMIGGYM
                     RKDGHHLNINVFNRDTLLDAQAHPEKYPTLTIRVSGYCVYFADLTKEQQDDVIARTFHA
                     SM"
     CDS             2595..3431
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 70.1;
                     E-value: 7.7e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg20_3"
                     /sec_met_domain="Radical_SAM (E-value: 2.5e-33, bitscore:
                     106.1, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSANPLLNPVPTKDGHVVGYVHSLETFGAVDGPGIRFVAFMQGCH
                     MRCKFCHNPDTWKTRVGSQMTTEEVLNKALPYRSFWGDKGGITLSGGEILLQIDFALEL
                     FKMCKEEGISTCLDTCGQPFTRREPWFSKFNELMDYTDILLVDIKHINSDEHKRLTGFP
                     NDNILDMCEYLSSIGKPVWIRHVLIPGITDNDEYLKQLGEYVKENLHNVEKFEVLPYHT
                     MGVHKYQEMGIRYRLEGVEPPTPERVKNAEKLLEVDRYQGYKDWYPGINTLDQQPS"
     CDS             complement(3517..4395)
                     /locus_tag="ctg20_4"
                     /transl_table=11
                     /translation="MYYNAALVLEGGAMRGLYTSGVLDTFLEHDIQFDCVIGVSAGSLN
                     GANFVSKQYQRTYNVNVKYRKDHDYISLARPFKHESIINLDFLFDDHGLDWHNFDNKAY
                     LKSETDFIVVATSLESGKAVTLRNPQPGQDLIDALEASSSMPFISKPHNTSKGLCLDGG
                     IANSIPFDIAQKMGFQKIIVVRTRPRDYRKKPTSFALKRLYSNSFKEYPEFVKTAIRRP
                     EMYNHSVEVLNKLEHNDKIFCLAPNDPVKVGRLEHNTKKMTELYNIGRNDAESNLEAML
                     NYLQKSEPLYD"
     CDS             complement(4653..4955)
                     /locus_tag="ctg20_5"
                     /transl_table=11
                     /translation="MNKIIISKLNNDENKIEWRISNSETGHYLNISISRALEDAMKKKR
                     NLSFNRFESEQINNLSHLVTNIQEDYVLNIDESNISSSYLPLKGIDALSYMKTVE"
     CDS             complement(5481..6056)
                     /locus_tag="ctg20_6"
                     /transl_table=11
                     /translation="VISGIFYFYILMKAWGKMTIHDSNVREKLLQLGSQKRYNFTAEYA
                     RCGYKVTYRRGLDRDKLAPTIMFKNVKINNNLVTDHLWFNYTKGFAELGKLIVGDVISF
                     NARVASYEKLGHKIDYKLERPTKVKLVPHKNGKDALPIPNTTKGKNELLGYIMLENKKF
                     YQENNRPYYPWYVEKYKKFLDQRSSNTV"
     CDS             complement(6075..6722)
                     /locus_tag="ctg20_7"
                     /transl_table=11
                     /translation="MKIAPSILSADMLNLGADVQKVEDAGAEYLHIDIMDGNFVPNLSF
                     GANVVKALRPQSKMVFDVHLMVNNPENWVEDFIKAGADIVGVHYEATPHIHRALDLIKK
                     NNAKAEVVINPGTPVSAIESVLSIVDQVLVMTVDPGFGGQKFIPETLEKIRQLHQIKEE
                     RNLNFDIEVDGGINEETVKLCREAGATVAVAGSYVFNSGNETHAERINKLRN"
     CDS             complement(6739..7422)
                     /locus_tag="ctg20_8"
                     /transl_table=11
                     /translation="MNQDELKQLVGTKAVEWIKDGMIVGLGTGSTVKYMVDALGKRVNE
                     EGLNIVGVTTSIRTAEQAKSLGIVIKDIDEVDHIDLTIDGADEISSDFQGIKGGGAALL
                     YEKIVATKSNKNMWIVDESKMVDDLGQFPLPVEVIPYGSGTVFKRFEEKGLNPEFRKNE
                     DGSLLHTDSDNYIIDLHLGKIENPKELGDYLINQVGVVEHGLFLDIVNTVIVGRQDGPE
                     VLEAR"
     CDS             complement(7740..8072)
                     /locus_tag="ctg20_9"
                     /transl_table=11
                     /translation="MLYNEKLVLNNIRKLRKEKKLTQDEVSKFLGIARGSYAKTENGIN
                     KITLDIVLKLSRLYNTSIEGLLCLSNDNNELDTVLKEGVTYNGKTLSSTDIIALKAFLQ
                     GLIANK"
     CDS             complement(8744..9022)
                     /locus_tag="ctg20_10"
                     /transl_table=11
                     /translation="LASQNLNIKVKLDFDESNLKSLNLGTVRVESDVNHFKSKLRSVLN
                     SNFKITPKINTKGISNRLSNIKNDMREFRNAAPIKINFELIKMYLVI"
     CDS             complement(9111..9686)
                     /locus_tag="ctg20_11"
                     /transl_table=11
                     /translation="MFCTKCGAKNPEDATFCYKCGNEMYTDKTRTMVDDTTDTTDEIVA
                     EEVNPLIDFWTNIVNFEGKMNRHNFWVAVFIMALINIVIVVISYGTEAMILWYLYDLFF
                     LIATLSASVRRLHDINKSGAYVLLNLIPLVGQVALLIMLLQENEVDKKKVVDDPINNMD
                     DKGFYLTAAVFIGIILVILLPLLLNGTL"
     CDS             complement(9700..10737)
                     /locus_tag="ctg20_12"
                     /transl_table=11
                     /translation="MCKKIANPEFMINSQLAKNSREQKRLEKLQANYPEAIEVAKYNVL
                     AIRYMFKLVLSKKELDKLQNIVKKCHNIVRRYISGKGSGTGDLSLYTKEFNRYKNWLMN
                     YHTDNLDTPSRFPTSILTNMIITGQKDEANKIYDNVSKSDGIITNDIAIKIKNEEFLNW
                     CLSNMLRFDGYEKHKITVSKFGEFNMVVGKRRNNYWYKVDSSIMLRMANYPKIKPLSVE
                     RLSLLKDGMIPTPETRKKLIADFILLLNNNKFHIYHPNLKRLSDRNYRKYSNNIYVYSN
                     KGDLAIFSIDDILNKIMLDYITTPNSVKLNIHNLVDNANKITVKELTQQIEKDIQIDQS
                     IKELF"
     CDS             complement(10722..11078)
                     /locus_tag="ctg20_13"
                     /transl_table=11
                     /translation="MMTKLLITNYERNVLINSYLLKNTPELDDIRRLLVHNKHISENDV
                     STEMARRIKNHKADWLRVTYLDLTKDKSRSSYYVKNGEKFTCYFCNKPLTSKAYFVTDK
                     DDKVFQVGSECVKK"
     CDS             11606..12481
                     /locus_tag="ctg20_14"
                     /transl_table=11
                     /translation="MTTALEVKNINKKYKNKVVLNDINFSIQSGEIVALIGKNGAGKST
                     LINIITKLIQQDSGQSKIFEKEKFDKNLIGVMMQENISLDRITVKEIIKLTRTYYRNPM
                     SYQAILALSELQNYTNHPMDKLSGGQKRKLQFALTLAGNPDLIFLDEPTVGMDAESRTK
                     FWKHVDELKKQGKTFLITSHYLEELEKVANRFIFLHNQKIIFDGSLNEMGKQLKKVQVT
                     FNSELIEDIFNKLPAVLRVSELNHHYTLITSDVNRLITQLVPYLSAIDNLEIRQQNLDT
                     LMDSLIRNEE"
     CDS             12484..13233
                     /locus_tag="ctg20_15"
                     /transl_table=11
                     /translation="MTNFLYQLEIDFKRIILRNPAFFMFDLAMPLTFYLLFTKALPHYS
                     NTMQWKVDYLVSMAIYGILLGSIMTVAHTLSSDIDRKFTLFIDLSPFPKFMYYIEMIII
                     FEVLNLLCLVGIGLAGILVNNLTLPTKVWILLLILIPIMSLPLVLLGILISSFKDSNVI
                     NALANILVFPMAICSGLWWPLSVLPQWLQKIGKVTPTYYISNLSNNILHGNSINYNSLV
                     GLIIWFVVLVLALGISYIFTSKKDWKN"
ORIGIN
        1 tagttggttt tacttcaaca atttcagttt cttccattgt ataaaaaatc ctcctatact
       61 ttttatgata atagtatagg gggatttcaa attttttcat attcagctca ttataaagta
      121 cttactcaaa aatattgtca tattaacatt tataatgatt aaaataaatg atagcgttta
      181 taagattttt tgaaaataaa aaacattgtg aacgattttt cattgaattt aatttaacta
      241 aggtgtagta ttaacagtgt agaaaacgtt ttcttgaaaa tcacaaagga ggctttgagt
      301 atggttgaaa agactcaaat gcaaaacgtt gatgtaaaag atgcttggga aggatttaga
      361 ggaaaagaat ggaaagaaga aattgatatt cgtgaattta ttcaagataa cttcactcca
      421 tatgatggag atcaatcttt ccttgaagga ccaaccgaag ctacaacgaa gttaaacgat
      481 aaattaatcg acttaaaatt aaaagaacgt gcacatggtg gacctcttga agctgataca
      541 aaagtagtat caacaattac atctcataaa ccaggttatt tagacaagtc attagaaaag
      601 attgttggtt tacaaacaga taaacctatg aaacgtgcac ttatgccata tggtggtatc
      661 agaatggcta aaggtgcttt ggaagcttat ggattcaaga ttgatccaga aacagacttc
      721 gtatttgaaa atctatctaa gacgcataac caaggtgtat ttgatgcata cacaagcgaa
      781 atcatgaaag ctcgccacaa caagattatc actggactac cagatgcata tggtcgtggc
      841 cgtttgattg gagacttccc acgtattgca ttatacggta ttgatcgttt gatcgaagga
      901 aagaagaagg atttggaaaa ttgcggcgat ggtgaaatga ctaacgacgt aatccaaatg
      961 cgtgaagaaa tttctgacca aatcaaagcc ttaaacgata tgaagattat ggctgaatcc
     1021 tatggctatg acatttctaa accagctaca acagctaaag aagcaattca atggctatac
     1081 tttggttact tagcatctat caagcaacaa aatggtgctg ctatgtctat tggtcgtatc
     1141 gattcattca tcgatattta cattgaacgt gatatgaagc gtggtatttt aactgaaaaa
     1201 gaagcacaag aattaattga ccaattcaca atgaaattga gaatggtatg cttcattcgt
     1261 acaccagctt acaactcact attctcaggt aaccctatct gggcaacatt atcaattgct
     1321 ggtatgggct tagatggacg tcaccatgta actaagacat catatcgttt cttaaataca
     1381 ttgcataata tgggtgccgc accagaacca aatattacat tactttggtc tgatcgtttg
     1441 cctgaatcat ttaagcacta tgctgcaagt gtatctattg caagttctac aattcaatac
     1501 gaaaacgatg acttaatgat gaagacatgg ggaacagact actatggcat tgcttgctgt
     1561 gtatctgcac aaccaattgc aaatggtatg caattcttcg gagctcgtgc aaatctagct
     1621 aaggctgtat tgtatgctat caatggtggt aaggatgaac ttacaaaaca acaagtaggt
     1681 cctgcatacg aaccaattag ctcagaatac attgactacg atgaattcat gaagaaattc
     1741 gatgacatga tggaatggat tgctgatatt tacgtaaatg ctttgaacac aattcattac
     1801 atgcatgaca agtattacta tgaatcagca caaatggcat tgaaagatac attggtagat
     1861 cgtagctttg ctacaggtat ctctggattg tctgttgctg ctgactcaat ttcagctatc
     1921 aagtacggac atgttaaagt aattcgtgac gaagatggta ttgctgttga cttcaaggca
     1981 gatcatgact tccctcgtta tggtaacgat gatgatcgtg ttgacgatat tgctgcatgg
     2041 cttgttaagg aactatttac taagatgagc aagcatcatc tatatcgtgg agctaagtta
     2101 acaacatccg ttcttacaat tacttcaaat gttgtttatg gtaagaacac aggtactact
     2161 ccaaatggtc gtcaaaaggg tgaaccattt gcaccaggtg ctaacccagc atacggagca
     2221 gatacaacag gtgctttagc atcactatta tccgttgcta aattaccata caagtatgct
     2281 acagatggta tttctaatac atttgctgta acaccaacaa cattgggtca tgatgaaaag
     2341 atgcaagaag atacattggt aaacatgatc ggaggctata tgagaaaaga tggtcatcat
     2401 ttgaatatta atgtattcaa cagagatact ttattagatg ctcaagcaca tccagagaag
     2461 tatccaacat taacaattcg tgtttcaggt tactgtgtat actttgctga tttaactaaa
     2521 gaacaacaag atgacgttat tgcaagaacc ttccatgctt caatgtaata attagaagag
     2581 agtaggtgga gagaatgagt gctaatcctt tattgaatcc agttccaact aaagacggtc
     2641 atgtcgtagg atatgtacac tctttggaaa cttttggagc tgttgatgga ccaggtatac
     2701 gttttgtcgc ttttatgcaa ggttgccata tgaggtgtaa gttctgtcac aatccggaca
     2761 cttggaaaac aagggtaggt tcacaaatga cgactgaaga agttctaaac aaggcacttc
     2821 cttatcgcag cttttggggc gacaaaggtg gaataacttt atcaggtggt gaaattctat
     2881 tgcaaataga ttttgcacta gagttattca agatgtgcaa ggaagaaggt attagcactt
     2941 gtttggacac ttgtggacag ccatttacaa gaagagaacc ttggtttagt aagttcaatg
     3001 agttaatgga ctataccgac attttattag ttgatatcaa gcatattaat tcggacgaac
     3061 ataagagatt aaccggattc ccaaatgata atattttgga tatgtgcgaa tatctttcta
     3121 gtatagggaa acctgtttgg attcgtcatg tccttatccc aggcatcacg gataatgatg
     3181 aatacttgaa acaattgggc gagtatgtta aggaaaatct tcataatgtc gaaaaatttg
     3241 aagtattacc ttatcataca atgggcgttc ataagtatca ggaaatgggt atcagatatc
     3301 gtcttgaagg tgttgagcct cctacgccag aaagagtaaa aaatgctgaa aaactgctag
     3361 aagtagatag atatcaagga tataaagatt ggtatccagg cattaatact ttggatcaac
     3421 aaccaagcta gctaaattgg caaatataaa ataaaaacat ggtatcgctt agttttgcta
     3481 agtagatacc atgttttttg gttttaaaat gtatttttag tcatataggg gttcactttt
     3541 ttgaagatag ttaagcattg cttcaagatt actttcagca tcatttctac ctatattata
     3601 gagttcagtc atctttttag tattgtgttc aagcctacca actttaactg gatcattggg
     3661 tgctaaacaa aaaattttgt cgttgtgttc aagtttattt aaaacttcaa cagaatggtt
     3721 atacatttca ggacgtctga tagccgtttt aacgaattct gggtattcct tgaagctatt
     3781 actgtagagg cgttttaaag caaaactagt aggctttttg cgataatcac gtgggcgagt
     3841 acgaacaact ataatttttt gaaaacccat tttttgagcg atatcaaaag ggatagagtt
     3901 agcaatacca ccatctagac aaagtccttt agaagtatta tgtggctttg agatgaaagg
     3961 catagatgaa gaagcttcta aggcatcaat taaatcttga ccaggttggg gattacgtag
     4021 cgttacagct tttccgcttt ctagagaagt agctacaaca ataaaatcag tttcagactt
     4081 taaataggct ttattatcga aattatgcca atctaatcca tgatcatcaa ataagaaatc
     4141 taagttaata atactttcat gcttaaaagg gcgtgctaag gagatatagt catgatcttt
     4201 acggtattta acatttacat tataagttct ttgatattgt tttgaaacga aattagcgcc
     4261 atttagagac cctgcagaaa ctccaataac acaatcaaat tgaatatcat gttccaaaaa
     4321 tgtatctaga acgccagaag tatagaggcc acgcattgca ccaccttcta aaactagagc
     4381 tgcattgtaa tacatgtaac cattcctttc gaaaaataac tagaattaat ataaacctaa
     4441 aaagtagaga tagcaagaat acaaaggaat aataagagaa tttaaatatt taattagtca
     4501 aaaacaatgc aactgtaaaa tatttaacaa aaaaacaacc tctatcatca aaatagaggc
     4561 tgttgacaga taaatcttta atcaattaag aatacttaag tatatccaag ataagtatta
     4621 aaataacaac ttaatttaat gcttataatc acttattcga cggttttcat atatgataat
     4681 gcgtcgatac cctttaatgg taaataacta gaactaatat ttgattcatc aatattaaga
     4741 acgtagtctt cttgaatgtt tgtaactaag tgagacaaat tgttaatttg ttcagattca
     4801 aagcggttaa aggataaatt acgctttttc ttcatagcat cttctaatgc acgggaaatt
     4861 gaaatattta agtaatgtcc agtttcactg ttagaaatac gccattcaat cttattttcg
     4921 tcattgttta acttgctaat aataatttta ttcatatata attaccacct taataaatgt
     4981 aaatgctttc cttaacttac aagtataact ctactctcat ttacgaataa aagcaagggt
     5041 aaaattaaaa atataagtaa ataattgttt ttgcttattt aaaaagttga atatcaatat
     5101 aatataagga ttggtatgtt aatataattg ttttgatgaa gaattattga atggaatcag
     5161 ttgttatagc caaattaaaa gtatgcaaat ttgttttttt ataaatagca gattgtagat
     5221 agattattaa agtcaaactt aggaaatcaa tttttgataa aataaaaagt tattttaact
     5281 ttctgaaaaa atatgatttt tgataaacat caaaattaat caataaagct ataatgagat
     5341 tatattacac tgtttggatt tctctctatc catctttatg aaaaacgtac attttttaga
     5401 aagatgaatt aagaattaat catgacaaaa aagcccccta atctttatag taaattggag
     5461 actttaaagt gtatctattg ttaaacagtg ttagaagatc tttgatctaa gaatttttta
     5521 tacttttcaa cataccatgg gtaatatgga cgattatttt cttgataaaa ttttttattc
     5581 tctagcataa tgtatcccaa caactcattt ttaccttttg tagtgttagg tataggcaaa
     5641 gcatctttac catttttatg gggaactaat ttaacttttg ttggacgttc taatttataa
     5701 tctattttat gtcctaattt ctcatagcta gcaactcttg cattaaaact aataacatcc
     5761 ccaacaatta gttttcccaa ctcagcaaag ccttttgtgt agttgaacca cagatggtct
     5821 gtaactagat tgttatttat cttaacattt ttgaacataa ttgttggagc taatttatct
     5881 ctatctaatc cacgcctata tgtaactttg taaccacacc ttgcatattc agctgtaaaa
     5941 ttgtaccgtt tttgacttcc taattgaagt aatttttccc gaacattcga atcatgtata
     6001 gtcatttttc cccacgcttt catcaatata taaaaataga agataccact aatcacagta
     6061 gtatcttcta tatattaatt acgtaattta tttattcttt cagcatgagt ttcattacca
     6121 gaattaaaaa cgtatgatcc ggcaaccgca actgttgcgc cagcttctcg acataactta
     6181 acggtttctt cgttaattcc accatcaact tctatatcaa agttaagatt tctttcttct
     6241 tttatttgat gtagttggcg aatcttttct aatgtttctg gtatgaattt ttgtccacca
     6301 aatccaggat caactgtcat aacaagtact tgatcaacaa ttgatagaac actttcaatt
     6361 gcactaactg gtgttcctgg attaattact acttcagcct tagcattgtt tttcttgatt
     6421 aaatctaatg cacgatgaat gtgtggtgta gcttcataat gaacacctac aatgtctgca
     6481 ccagctttta tgaagtcttc tacccaattt tcagggttat tcaccatcaa atgaacgtca
     6541 aacaccattt tactttgtgg tcttaatgct ttaactacat tagcaccaaa agacagattg
     6601 ggaacaaaat ttccgtccat aatatcaata tggagatatt cagcaccggc atcttctacc
     6661 ttttgtacat cagcacctag atttaacata tcagcgctaa gaatagaagg agctattttc
     6721 atataaattc acctcgaatt atctggcttc aagaacctca gggccatctt gacgcccaac
     6781 aataacagta tttacgatgt ctaagaataa tccgtgttcg acaactccta cttgattaat
     6841 taggtaatct ccaagttctt taggattttc aattttacct aagtgaagat cgataatata
     6901 gttatcagag tctgtatgaa gtaaactgcc atcttcattt tttctaaatt ctggatttaa
     6961 tcctttttct tcaaagcgtt taaagacagt accactacca taagggataa cttcaactgg
     7021 taatgggaat tgtcctagat catcgaccat tttactttca tcgacaatcc acatattctt
     7081 attagattta gttgcaacaa tcttttcata tagtaaagct gcaccgccac ctttaattcc
     7141 ttggaagtca gaagaaattt cgtcagcacc atcgattgtt aaatcaatat gatcaacttc
     7201 atcaatatct ttgataacaa tgcccaaaga cttagcttgt tcagctgttc taatagaagt
     7261 tgtaacacca acaatgttta gaccttcttc gtttacacgt tttccaagtg catcaaccat
     7321 gtatttaaca gttgaacctg ttcctagacc gacaatcatg ccatctttaa tccattcaac
     7381 tgcctttgta cctactaatt gctttaattc atcttggttc attaattata cccctttcaa
     7441 acatcgcata cctaaattat acaacaatta gtatagttcc ggcaccatga atagtaagta
     7501 ttttcaaaat tattctaatt atttacttaa tagtattaat tccaagcatc ttaatagttc
     7561 ttttaacaat taacaacaag cgtatttata attacggtag ttattccatt acatatgtta
     7621 atataaacgc cttaaatcat tgccgatgtt ataaggataa gcaacagcat tacaaataac
     7681 ataaagtccc ctacttgctt tggagatatt gtctagctac tcttacacta ttttataatt
     7741 tacttattag ctattagacc ctgtaaaaag gctttgagcg cgattatatc agtacttgaa
     7801 agtgttttgc cattgtatgt aacaccttct tttaatacgg tatctaattc attgttatca
     7861 ttagatagac ataataaacc ctcaatactt gtattgtaca atctgcttaa ttttaaaact
     7921 atatcaaggg ttattttatt aatgccgttt tctgtttttg cataagaccc acgagcaata
     7981 cctaaaaatt tagatacttc atcttgtgtt aacttctttt cttttcttag ttttctaata
     8041 ttgttaagca ccaatttttc attgtataac atataataat atcgtccttt tgattttgta
     8101 tatatttagt agaaacacac gtaagtttct ggtttttatt ataccgtaca aacgttatta
     8161 tatcaaagtt tatagcaata accacatact taatataatc acgtgtaaac tctttaacaa
     8221 taaaggtata cttttgttaa gactgcttgt actttgaata caaagcatct gtaagctctc
     8281 atcattctag ttaattatat aatagtcatc gcgacgtatt gaacaaggta acccttgagt
     8341 ttcctcgtta taattccaat gttaaggggt aactataaag taccccaagt agcaataaac
     8401 atataagaat acaaaaaata ccaccttctt actaagtaga tttatacaat caattttaac
     8461 aagatagtgg tattctatga aaaatgttga cgtcaacacg gttacatttt attgtacttt
     8521 agttagtgca ttgtatccga tgttgaaaga tatattttgc taatgttaaa gctttaaaac
     8581 gcttagttga taagcatgaa cattagtatc caatcatagt gagttagaaa ctcgtacttt
     8641 ctaacttact atgattattg tagtacttga aaacactcta tacaatacta atataatctt
     8701 aattcaccgc gatgtatctt ctctaattgt cttaatacaa cagctatatc actaaataca
     8761 tctttattaa ctcaaaatta attttgatag gagcagcgtt tctaaattcc ctcatatcat
     8821 tcttgatgtt gcttagtcgg ttagatatac cttttgtgtt tatctttgga gtaatcttaa
     8881 aattactatt caaaactgac cttaacttac tcttaaagtg atttacatct gattctaccc
     8941 ttactgtacc taggtttaaa ctctttaagt tcgactcgtc aaagtctagc ttaactttta
     9001 tgtttaagtt ttgactagcc aatcatttca aaaacatctt attattttat tagtaagcga
     9061 tcacatattg gcgatatata aaagcctaat tactagggct taaatattaa ttataaagta
     9121 ccattgagta ataatggcaa aagtattact aagataatgc ctataaaaac agcagctgta
     9181 agataaaaac ctttgtcatc catattattt atagggtcgt ctacgacctt ctttttatct
     9241 acttcgtttt cttgcaacaa cataatcagc aatgctactt gtccaacaag cggtataaga
     9301 ttaagtaata catatgcacc gcttttattt atatcgtgta atcttctcac ggaagcagat
     9361 agtgttgcaa taaggaagaa taggtcgtaa agataccata atatcatagc ttctgtacca
     9421 tatgatatca ctacaattac tatgttaata agtgccatta tgaacacagc aacccagaag
     9481 ttatgtctat tcattttacc ttcaaaatta acaatattag tccaaaaatc aattagggga
     9541 ttaacttctt ctgctacaat ttcgtcagta gtatcagtag tatcatctac catagttctt
     9601 gttttatctg tgtacatttc atttccacat ttataacaaa aagttgcatc ttctgggttc
     9661 tttgctccgc atttagtaca aaacatattt tatctccttt taaaataatt cctttattga
     9721 ttggtcaatt tgtatatctt tttcaatttg ttgtgtcaat tcttttacag taattttatt
     9781 cgcattatct acgaggttgt gtatgtttaa tttaacagaa tttggtgtag taatataatc
     9841 taacattatt ttatttaaaa tatcatcaat actaaaaatc gctaaatctc ctttattcga
     9901 atatacatat atattattag aatattttct gtaatttctg tcagataacc tttttaaatt
     9961 tggatggtat atgtggaatt tattattgtt taataacaat atgaaatctg ctatcaattt
    10021 tttacgtgtt tcaggagttg gaatcattcc atcttttaat aatgataatc gttcgactga
    10081 caatggcttt attttgggat aatttgccat ccttaacatg atagaagaat caactttata
    10141 ccaataatta ttgcgtcttt taccaactac catattaaat tcaccaaact tcgataccgt
    10201 tatcttatgt ttttcatatc catcaaatct tagcatattt gatagacacc aatttaaaaa
    10261 ttcttcattt ttaattttaa tagctatatc attagtaata attccatcac ttttactaac
    10321 attatcatat atcttatttg cttcatcttt ttgacctgta ataatcatgt ttgttagtat
    10381 agaagtggga aatctcgatg gtgtatctag attatctgtg tgataattca tcaaccaatt
    10441 tttatatcta ttgaactcct ttgtgtaaag actcaaatcc cctgttccac taccttttcc
    10501 actaatatac ctacggacta tattgtgaca tttcttaact atattttgta atttatccaa
    10561 ctctttcttg cttaaaacca atttgaacat atatctaata gctaacacat tatattttgc
    10621 tacttcaata gcttctggat aatttgcttg taatttttct aaccgttttt gctctctaga
    10681 gtttttagcc aattgtgaat taatcataaa ttctggattg gctatttttt tacacattct
    10741 gaaccaacct gaaaaacttt gtcatcctta tcagtaacaa aataagcttt tgatgtcaaa
    10801 ggtttattac aaaaatagca cgtaaatttt tcaccatttt ttacataata cgaactccta
    10861 cttttatctt ttgttaagtc caaatatgta actcttaacc aatcagcttt atgattcttg
    10921 atccttctag ccatttcagt tgacacatca ttttcactga tatgtttatt atgtacaagc
    10981 aatcttctga tatcatccaa ttcaggtgta ttttttaaaa gatagctatt aatcaaaaca
    11041 ttccgctcat agttagtaat taaaagtttt gtcatcatgt ttcacccttg taactacaat
    11101 atgtattata cacaccgctt gttcggtgct ttggtatttt aatccttctt tatattttat
    11161 gaaattatct tccttatagc ttgataactt cctcctttaa aaataaaaac gcaattctgt
    11221 attattgtgt gtgttatgaa aattagtcgt aactaatttc tttgctctcc tctgacaata
    11281 ataaatttga cttaataata ttatcaaaaa ttttattata catcaatata acgttcaact
    11341 catcgcgata aaataagcaa cataaaaaac tccttagcaa tgtaaagggc ttgtatagat
    11401 ttattttagg ctctgagtta ggtctgtatt tttacataat tacaagttat ttataaaaat
    11461 caccataatt cgaaattatt tatttaatag tattaattcc atacatctta tgacaattgt
    11521 cacgaagagt aatgactttc aattctatta accttaactg attttgttta tattgaagat
    11581 gtaatacagg gaaggatgat aaattatgac caccgcactt gaagtaaaaa atatcaataa
    11641 aaaatataaa aataaagttg ttttaaatga tattaatttt tcaattcaat ctggagaaat
    11701 tgttgcctta attggtaaaa atggtgctgg gaaatctact ttgatcaata ttattacgaa
    11761 gttaattcaa caagatagtg gacaaagcaa gatttttgag aaagaaaaat ttgataaaaa
    11821 tttaattgga gtaatgatgc aagaaaatat ctccttagat cgaataacag tgaaagaaat
    11881 aattaaatta actagaacat attatcgtaa tcctatgtct tatcaagcga tattagcttt
    11941 atcagagttg caaaattata caaaccatcc tatggataaa ttatctggag gtcaaaaaag
    12001 aaagctacag ttcgctctta ctttagccgg gaatccagat ttaattttct tagacgaacc
    12061 tactgtagga atggatgcag aaagtcgcac taaattttgg aagcatgtcg atgagttaaa
    12121 gaaacaaggg aaaacatttt taattactag tcattattta gaagaattag aaaaagttgc
    12181 taataggttt atttttctgc ataatcaaaa gattattttt gatggtagtt taaatgaaat
    12241 gggaaagcag ttaaaaaagg ttcaagtaac cttcaatagt gagttgatag aagatatttt
    12301 taataaatta ccagctgtac ttagagtaag tgaattgaac caccattata cacttattac
    12361 aagtgatgtt aatagattaa taactcagtt agtcccctat ttatcagcaa ttgataattt
    12421 agaaattaga caacaaaact tagacacatt aatggactct ttaattagaa acgaggaata
    12481 atgatgacca attttttata tcaacttgaa attgatttta aacgcataat cttaagaaat
    12541 ccggcattct tcatgtttga tttagcaatg cctttgactt tttatttact ttttacaaaa
    12601 gccttaccac attatagtaa tacaatgcaa tggaaggtag attatttagt aagtatggcg
    12661 atttacggaa ttctacttgg atctattatg actgttgcac atacactaag ttccgatata
    12721 gaccgcaaat ttacactatt tatagattta agccctttcc ctaaatttat gtattatatt
    12781 gaaatgatca ttatttttga agtattaaac ttactttgtc ttgtaggaat tggtttagcc
    12841 ggtattttag ttaataatct cacattaccg acaaaggtct ggatactttt attaattcta
    12901 attcctatta tgagtttgcc tctcgtttta ttaggaattt tgatttcatc atttaaagat
    12961 agtaatgtta tcaatgcctt agccaacatc ttagtttttc ctatggcaat ttgtagtggc
    13021 ttatggtggc cgcttagtgt acttccacaa tggctacaaa aaattggtaa agtaacacca
    13081 acatactaca tttcaaattt gtctaataac attttacatg gaaatagcat caactataat
    13141 tcgcttgtag gtttaataat ttggtttgtt gtgttagtct tagctctagg aattagttat
    13201 atatttactt caaaaaagga ttggaaaaac taatgaagaa gtttctaaaa aagcacgtat
    13261 tatttccaag ttacgtagga ctagctcctt atttctggtt actatggtta tttccagcag
    13321 taagtgtgtt acttacattg cccactatta tgcgtaatat aacgttaatt ttactaatta
    13381 tttttgtcaa agtttacaga gattgttttt tagccagaga taaaggagta g
//
