GCA_012028945.1 - 16 region(s)

Identified secondary metabolite regions using strictness 'relaxed'

VIOH01000073.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 2.1	betalactone,NRPS	1	27,159	fengycin	NRP	73%
Region 2.2	NRPS,transAT-PKS,T3PKS,PKS-like	101,696	206,896	bacillaene	Polyketide + NRP	100%

VIOH01000014.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 3.1	NRPS	1	9,424	surfactin	NRP:Lipopeptide	8%

VIOH01000015.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 4.1	NRPS	1	13,270	plipastatin	NRP	30%

VIOH01000001.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 6.1	other	319,907	361,325	bacilysin	Other	100%
Region 6.2	sactipeptide	368,165	389,776	subtilosin A	RiPP:Thiopeptide	100%
Region 6.3	CDPS	614,465	635,211
Region 6.4	lanthipeptide-class-ii,lanthipeptide-class-i	741,049	790,791	subtilin	RiPP:Lanthipeptide	100%
Region 6.5	NRPS	939,402	986,538	bacillibactin	NRP	100%

VIOH01000020.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 8.1	NRPS	1	10,013	plipastatin	NRP	23%

VIOH01000002.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 9.1	T3PKS	805,395	846,492
Region 9.2	terpene	894,229	916,127
Region 9.3	NRPS	985,945	1,007,590	plipastatin	NRP	30%

VIOH01000004.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 11.1	terpene	197,973	218,776

VIOH01000005.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 12.1	NRPS	155,949	183,669	surfactin	NRP:Lipopeptide	43%

VIOH01000009.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 16.1	NRPS	1	25,567	surfactin	NRP:Lipopeptide	43%

Compact view

VIOH01000073.1 - Region 1 - NRPS,betalactone

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Location: 1 - 27,159 nt. (total: 27,159 nt) Show pHMM detection rules used

Region on contig edge.

betalactone: (HMGL-like and AMP-binding)
NRPS: cds(Condensation and (AMP-binding or A-OX))

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 27159:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-tyr) + (ile)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg2_1

Search NORINE for peptide: strict or relaxed

AMP-binding (177..571): tyr

NRPSPredictor2: tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: phe, trp, phg, tyr, bht
Small clusters prediction:: tyr, bht
Single AA prediction:: tyr

Stachelhaus prediction details:

Stachelhaus sequence:: DGTITAEVAK
Nearest Stachelhaus code:: tyr
Stachelhaus code match:: 100% (strong)

ctg2_2

Search NORINE for peptide: strict or relaxed

AMP-binding (474..867): ile

NRPSPredictor2: ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: ile

Stachelhaus prediction details:

Stachelhaus sequence:: DAFFYGITFK
Nearest Stachelhaus code:: ile
Stachelhaus code match:: 100% (strong)

ctg2_6

Search NORINE for peptide: strict or relaxed

AMP-binding (13..445): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: N/A
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: gclhIAApvK
Nearest Stachelhaus code:: phe
Stachelhaus code match:: 40% (weak)

VIOH01000073.1 - Region 2 - NRPS,PKS-like,T3PKS,transAT-PKS

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Location: 101,696 - 206,896 nt. (total: 105,201 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))
transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))
T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)
PKS-like: (t2pks2 or ksIII)

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 2, location 101695 - 206896:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (gly - ccmal - ohmal - mal) + (ccmal - mal - ohmal - mal - mal) + (ccmal - ohmal - mal - mal) + (X - ccmal - ccmal - ccmal) + (mal - mal - ccmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 116336 - 206896:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (gly - ccmal - ohmal - mal) + (ccmal - mal - ohmal - mal - mal) + (ccmal - ohmal - mal - mal) + (X - ccmal - ccmal - ccmal) + (mal - mal - ccmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg2_118

Search NORINE for peptide: strict or relaxed

AMP-binding (497..905): X

NRPSPredictor2: hydrophobic-aliphatic

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVsnmAiIYK
Nearest Stachelhaus code:: ala
Stachelhaus code match:: 60% (weak)

ctg2_121

Search NORINE for peptide: strict or relaxed

CAL_domain (63..471): pk

minowa: fatty_acid

Prediction, score:

fatty_acid: 10.7
NH2: 10.3
shikimic_acid: 0.0
Acetyl-CoA: 0.0
AHBA: 0.0

AMP-binding (1159..1554): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGMIWK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 100% (strong)

ctg2_127

PKS_AT (4..288): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 66.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 96.2
inactive: 40.7
Methoxymalonyl-CoA: 29.2
Methylmalonyl-CoA: 22.2
Propionyl-CoA: 16.2
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ctg2_128

PKS_AT (7..293): pk

ATSignature: (unknown)

No matches above 50%

minowa: inactive

Prediction, score:

inactive: 27.3
Malonyl-CoA: 24.5
Isobutyryl-CoA: 8.5
Benzoyl-CoA: 8.4
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ctg2_131

PKS_AT (4..284): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 101.4
Methoxymalonyl-CoA: 55.6
inactive: 37.8
Methylmalonyl-CoA: 37.1
Propionyl-CoA: 24.9
Ethylmalonyl-CoA: 10.8
2-Methylbutyryl-CoA: 9.4
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

VIOH01000014.1 - Region 1 - NRPS

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Location: 1 - 9,424 nt. (total: 9,424 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 9424:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (val - asp)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg3_1

Search NORINE for peptide: strict or relaxed

AMP-binding (470..864): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: val

Stachelhaus prediction details:

Stachelhaus sequence:: DAFWIGGTFK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 100% (strong)

AMP-binding (1501..1904): asp

NRPSPredictor2: asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asp

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKVGHIGK
Nearest Stachelhaus code:: asp
Stachelhaus code match:: 100% (strong)

VIOH01000015.1 - Region 1 - NRPS

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Location: 1 - 13,270 nt. (total: 13,270 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

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Gene details

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 13270:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-orn) + (tyr - D-thr)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg4_2

Search NORINE for peptide: strict or relaxed

AMP-binding (474..865): tyr

NRPSPredictor2: tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: phe, trp, phg, tyr, bht
Small clusters prediction:: tyr, bht
Single AA prediction:: tyr

Stachelhaus prediction details:

Stachelhaus sequence:: DGTITAEVAK
Nearest Stachelhaus code:: tyr
Stachelhaus code match:: 100% (strong)

AMP-binding (1505..1903): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

ctg4_3

Search NORINE for peptide: strict or relaxed

AMP-binding (235..640): orn

NRPSPredictor2: orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: orn, lys, arg
Small clusters prediction:: orn, horn
Single AA prediction:: orn

Stachelhaus prediction details:

Stachelhaus sequence:: DVGEIGSIDK
Nearest Stachelhaus code:: orn
Stachelhaus code match:: 100% (strong)

VIOH01000001.1 - Region 1 - other

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Location: 319,907 - 361,325 nt. (total: 41,419 nt) Show pHMM detection rules used

other: (LmbU or Neocarzinostat or fom1 or bcpB or frbD or mitE or vlmB or prnB or CaiA or bacilysin or orf2_PTase)

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KnownClusterBlast

Similar known gene clusters

VIOH01000001.1 - Region 2 - sactipeptide

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Location: 368,165 - 389,776 nt. (total: 21,612 nt) Show pHMM detection rules used

sactipeptide: (subtilosin or thuricin or TIGR04404 or Subtilosin_A or skfc)

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KnownClusterBlast

Sactipeptides

Similar known gene clusters

Sactipeptide predictions

Putative Sactipeptide(s)

ctg6_376 leader / core peptide: MKKAVIVENK - GCATCSIGAACLVDGPIPDFEIAGATGLFGLWG

Sactipeptides

Core peptide predictions

ctg6_376: Putative sactipeptide; RODEO score: 33.0; Core sequence, approximate: GCATCSIGAACLVDGPIPDFEIAGATGLFGLWG

VIOH01000001.1 - Region 3 - CDPS

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Location: 614,465 - 635,211 nt. (total: 20,747 nt) Show pHMM detection rules used

CDPS: (CDPS or cycdipepsynth)

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KnownClusterBlast

Similar known gene clusters

No matches found.

VIOH01000001.1 - Region 4 - lanthipeptide-class-i,lanthipeptide-class-ii

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Location: 741,049 - 790,791 nt. (total: 49,743 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)
lanthipeptide-class-i: ((LANC_like and (Lant_dehydr_N or Lant_dehydr_C)) and not (YcaO or TIGR03882))

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Lanthipeptides

KnownClusterBlast

Lanthipeptide predictions

Lanthipeptide(s) for ctg6_730 - Putative Class II

ctg6_729 leader / core peptide: MKKTKILKTPELKVTKDLNLIDPCGDVLEEIKEHEVSGA - FNDhbWNDhbDhbDhbDhbDhaVGDhbILVDhaEVFGNKGKVCDhbGDhbKECQGDhbC
ctg6_731 leader / core peptide: MKKDMVKNASGFIEEDELISLANGENATGG - GDhbPVDhbAIIDhbALDhbGDhbDhbFDhbVDhbLDhaAADhaCPDhbDhaACDhbNLCNK
allorf_0749513_0749689 leader / core peptide: LFLNTVETS - LDhaLRIDhaYNIRLDIFNGRDhbEHGKFQKRCDhaIFKDhaHDNRMNKVMIDhbDhbMEHCV
allorf_0749747_0749923 leader / core peptide: LEGILGVKSFLENGQQSFKTLRDGCHNLRVTVGS - ANNGCLEDhaRKHDhbDhaNNGIYENDLDhaDhb
allorf_0749892_0750023 leader / core peptide: MAYMKMICPHDKGEPLYDMEPEVFGG - NLVDhbADhaDhaVDhaCLELRGKY

Lanthipeptide(s) for ctg6_732 - Putative Class II

ctg6_729 leader / core peptide: MKKTKILKTPELKVTKDLNLIDPCGDVLEEIKEHEVSGA - FNDhbWNDhbDhbDhbDhbDhaVGDhbILVDhaEVFGNKGKVCDhbGDhbKECQGDhbC
ctg6_731 leader / core peptide: MKKDMVKNASGFIEEDELISLANGENATGG - GDhbPVDhbAIIDhbALDhbGDhbDhbFDhbVDhbLDhaAADhaCPDhbDhaACDhbNLCNK
allorf_0749513_0749689 leader / core peptide: LFLNTVETS - LDhaLRIDhaYNIRLDIFNGRDhbEHGKFQKRCDhaIFKDhaHDNRMNKVMIDhbDhbMEHCV
allorf_0749747_0749923 leader / core peptide: LEGILGVKSFLENGQQSFKTLRDGCHNLRVTVGS - ANNGCLEDhaRKHDhbDhaNNGIYENDLDhaDhb
allorf_0749892_0750023 leader / core peptide: MAYMKMICPHDKGEPLYDMEPEVFGG - NLVDhbADhaDhaVDhaCLELRGKY

Lanthipeptide(s) for ctg6_751 - Putative Class I

ctg6_754 leader / core peptide: MSKFDDFDLDVVKVSKQDSKITPQ - WKDhaEDhaLCDhbPGCVDhbGVLQDhbCFLQDhbIDhbCNCKIDhaK

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Similar known gene clusters

Lanthipeptides

Core peptide predictions

ctg6_729 - Class II

Cleavage pHMM score: -3.20

RODEO score: 0

Monoisotopic mass: 3712.9 Da

Molecular weight: 3715.1 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 3733.2 Da N = 1; 3751.2 Da N = 2; 3769.2 Da N = 3; 3787.2 Da N = 4; 3805.2 Da N = 5; 3823.3 Da N = 6; 3841.3 Da N = 7; 3859.3 Da N = 8

ctg6_731 - Class II

Cleavage pHMM score: -5.20

RODEO score: 0

Monoisotopic mass: 3294.8 Da

Molecular weight: 3296.8 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 3314.8 Da N = 1; 3332.8 Da N = 2; 3350.8 Da N = 3; 3368.9 Da N = 4; 3386.9 Da N = 5; 3404.9 Da N = 6; 3422.9 Da N = 7; 3440.9 Da N = 8; 3459.0 Da N = 9; 3477.0 Da N = 10

allorf_0749513_0749689 - Class II

Cleavage pHMM score: -6.40

RODEO score: 0

Monoisotopic mass: 5740.0 Da

Molecular weight: 5743.7 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 5761.7 Da N = 1; 5779.7 Da N = 2; 5797.7 Da N = 3; 5815.7 Da N = 4; 5833.8 Da N = 5

allorf_0749747_0749923 - Class II

Cleavage pHMM score: -5.00

RODEO score: 0

Monoisotopic mass: 2546.2 Da

Molecular weight: 2547.7 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2565.7 Da N = 1; 2583.7 Da N = 2; 2601.7 Da N = 3; 2619.7 Da N = 4

allorf_0749892_0750023 - Class II

Cleavage pHMM score: -5.70

RODEO score: 0

Monoisotopic mass: 1767.0 Da

Molecular weight: 1768.0 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1786.0 Da N = 1; 1804.1 Da N = 2; 1822.1 Da N = 3

ctg6_729 - Class II

Cleavage pHMM score: -3.20

RODEO score: 0

Monoisotopic mass: 3712.9 Da

Molecular weight: 3715.1 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 3733.2 Da N = 1; 3751.2 Da N = 2; 3769.2 Da N = 3; 3787.2 Da N = 4; 3805.2 Da N = 5; 3823.3 Da N = 6; 3841.3 Da N = 7; 3859.3 Da N = 8

ctg6_731 - Class II

Cleavage pHMM score: -5.20

RODEO score: 0

Monoisotopic mass: 3294.8 Da

Molecular weight: 3296.8 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 3314.8 Da N = 1; 3332.8 Da N = 2; 3350.8 Da N = 3; 3368.9 Da N = 4; 3386.9 Da N = 5; 3404.9 Da N = 6; 3422.9 Da N = 7; 3440.9 Da N = 8; 3459.0 Da N = 9; 3477.0 Da N = 10

allorf_0749513_0749689 - Class II

Cleavage pHMM score: -6.40

RODEO score: 0

Monoisotopic mass: 5740.0 Da

Molecular weight: 5743.7 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 5761.7 Da N = 1; 5779.7 Da N = 2; 5797.7 Da N = 3; 5815.7 Da N = 4; 5833.8 Da N = 5

allorf_0749747_0749923 - Class II

Cleavage pHMM score: -5.00

RODEO score: 0

Monoisotopic mass: 2546.2 Da

Molecular weight: 2547.7 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2565.7 Da N = 1; 2583.7 Da N = 2; 2601.7 Da N = 3; 2619.7 Da N = 4

allorf_0749892_0750023 - Class II

Cleavage pHMM score: -5.70

RODEO score: 0

Monoisotopic mass: 1767.0 Da

Molecular weight: 1768.0 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1786.0 Da N = 1; 1804.1 Da N = 2; 1822.1 Da N = 3

ctg6_754 - Class I

Cleavage pHMM score: 27.80

RODEO score: 30

Monoisotopic mass: 3346.7 Da

Molecular weight: 3349.0 Da

Number of bridges: 5

Alternative weights: (assuming N unmodified Ser/Thr residues); 3367.0 Da N = 1; 3385.0 Da N = 2; 3403.0 Da N = 3

VIOH01000001.1 - Region 5 - NRPS

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Location: 939,402 - 986,538 nt. (total: 47,137 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 5, location 939401 - 986538:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (gly - thr)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg6_937

Search NORINE for peptide: strict or relaxed

AMP-binding (31..435): dhb

NRPSPredictor2: dhb

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: dhb, sal
Small clusters prediction:: dhb, sal
Single AA prediction:: dhb

Stachelhaus prediction details:

Stachelhaus sequence:: PLPAQGVVNK
Nearest Stachelhaus code:: dhb
Stachelhaus code match:: 100% (strong)

ctg6_939

Search NORINE for peptide: strict or relaxed

AMP-binding (463..861): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGLIWK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 100% (strong)

AMP-binding (1519..1935): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

VIOH01000020.1 - Region 1 - NRPS

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Location: 1 - 10,013 nt. (total: 10,013 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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Gene details

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 10013:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-val) + (pro - glu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg8_1

Search NORINE for peptide: strict or relaxed

AMP-binding (237..630): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: val

Stachelhaus prediction details:

Stachelhaus sequence:: DVFWIGGTFK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 100% (strong)

ctg8_2

Search NORINE for peptide: strict or relaxed

AMP-binding (469..866): pro

NRPSPredictor2: pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: pro, pip
Small clusters prediction:: pro
Single AA prediction:: pro

Stachelhaus prediction details:

Stachelhaus sequence:: DVQFIAHVVK
Nearest Stachelhaus code:: pro
Stachelhaus code match:: 100% (strong)

AMP-binding (1500..1901): glu

NRPSPredictor2: glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: glu, gln
Single AA prediction:: glu

Stachelhaus prediction details:

Stachelhaus sequence:: DAWHFGSVEK
Nearest Stachelhaus code:: glu
Stachelhaus code match:: 100% (strong)

VIOH01000002.1 - Region 1 - T3PKS

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Location: 805,395 - 846,492 nt. (total: 41,098 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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KnownClusterBlast

Similar known gene clusters

No matches found.

VIOH01000002.1 - Region 2 - terpene

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Location: 894,229 - 916,127 nt. (total: 21,899 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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KnownClusterBlast

Similar known gene clusters

No matches found.

VIOH01000002.1 - Region 3 - NRPS

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Location: 985,945 - 1,007,590 nt. (total: 21,646 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

VIOH01000004.1 - Region 1 - terpene

Download region GenBank file

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Location: 197,973 - 218,776 nt. (total: 20,804 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

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Gene details

Select a gene to view the details available for it

KnownClusterBlast

Similar known gene clusters

No matches found.

VIOH01000005.1 - Region 1 - NRPS

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Location: 155,949 - 183,669 nt. (total: 27,721 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 155948 - 183669:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (glu - leu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg12_170

Search NORINE for peptide: strict or relaxed

AMP-binding (466..867): glu

NRPSPredictor2: glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: glu, gln
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAKDLGVVDK
Nearest Stachelhaus code:: glu
Stachelhaus code match:: 100% (strong)

AMP-binding (1510..1908): leu

NRPSPredictor2: leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: leu

Stachelhaus prediction details:

Stachelhaus sequence:: DAFMMGMVFK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 100% (strong)

VIOH01000009.1 - Region 1 - NRPS

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Location: 1 - 25,567 nt. (total: 25,567 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 25567:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (leu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg16_2

Search NORINE for peptide: strict or relaxed

AMP-binding (469..867): leu

NRPSPredictor2: leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: leu

Stachelhaus prediction details:

Stachelhaus sequence:: DAFFLGCVFK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 100% (strong)