LOCUS       c00162_JACBJX0..        4221 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  JACBJX010000246.1 Ligilactobacillus salivarius strain FFIG124
            Lactobacillus_salivarius_FFIG124_contig_246_1, whole genome shotgun
            sequence.
ACCESSION   JACBJX010000246
VERSION     JACBJX010000246
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2022-06-21 19:15:51
            Original ID  :: JACBJX010000246.1
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4221
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4221
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[90:3221]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      91..3221
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4221
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4221
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(91..927)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.4;
                     E-value: 1.5e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg162_1"
                     /sec_met_domain="Radical_SAM (E-value: 1.3e-34, bitscore:
                     107.7, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSANPLLNPVPTKDGHVVGYVHSLETFGAVDGPGIRFVVFMQGCH
                     MRCKFCHNPDTWKTRVGSQMTTEEVLNKALPYRSFWGDKGGITLSGGEILLQIDFALEL
                     FKMCKEEGISTCLDTCGQPFTRREPWFSKFNELMDYTDILLVDIKHINSDEHKRLTGFP
                     NDNILDMCEYLSSIGKPVWIRHVLIPGITDNDEYLKQLGEYVKENLHNVEKFEVLPYHT
                     MGVHKYHEMGIRYRLEGVEPPTPERVKNAEKLLEVDRYQGYKDWYPGINTLDQQPS"
     CDS             complement(954..3221)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg162_2"
                     /sec_met_domain="Gly_radical (E-value: 9.8e-42, bitscore:
                     129.7, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 2.6e-262, bitscore:
                     859.1, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVEKTQMQNVDVKDAWEGFRGKEWKEEIDIREFIQDNFTPYDGDQ
                     SFLEGPTEATTKLNDKLIDLKLKERAHGGPLEADTKVVSTITSHKPGYLDKSLEKIVGL
                     QTDKPMKRALMPYGGIRMAKGALEAYGFKIDPETDFVFENLSKTHNQGVFDAYTSEIMK
                     ARHNKIITGLPDAYGRGRLIGDFPRVALYGIDRLIEEKKKDLENCGDGEMTNDIIQMRE
                     EISDQIKALNDMKIMAESYGYDISKPATTAKEAIQWLYFGYLASIKQQNGAAMSIGRID
                     SFIDIYIERDMKRGILTEKEAQELIDQFTMKLRMVCFIRTPAYNSLFSGNPIWATLSIA
                     GMGLDGRHHVTKTSYRFLNTLHNMGAAPEPNITLLWSDRLPESFKHYAASVSIASSTIQ
                     YENDDLMMKTWGTDYYGIACCVSAQPIANGMQFFGARANLAKAVLYAINGGKDELTKQQ
                     VGPAYEPISSEYIDYDEFMKKFDDMMEWIADIYVNALNTIHYMHDKYYYESAQMALKDT
                     LVDRSFATGISGLSVAADSISAIKYGHVKVIRDEDGIAVDFKADHDFPRYGNDDDRADD
                     IAAWLVKELFTKMSKHHLYRGAKLTTSVLTITSNVVYGKNTGTTPNGRQKGEPFAPGAN
                     PAYGADTTGALASLLSVAKLPYKYATDGISNTFAVTPTTLGHDEKMQEDTLVNMIGGYM
                     RKDGHHLNINVFNRDTLLDAQAHPEKYPTLTIRVSGYCVYFADLTKEQQDDVIARTFHT
                     SM"
     CDS             complement(3532..4116)
                     /locus_tag="ctg162_3"
                     /transl_table=11
                     /translation="VKVMHVLRVKTNFFWDSLNVIRASILGANDGIISVSGIVLGAAGA
                     NLDSKTLLISGLSGMLAGACSMAGGEWISVSTQRDIQEKTMERQSQLENDDCENCPIKL
                     QKNDILMPFHAAASSFCSFIIGAMIPLLTMILARPEHRVVFTLIAMIASLSINAIVCTH
                     NSEVSTSKTILRNVITGLLTTLVTFILGASV"
ORIGIN
        1 cctttttgac aaagaacccc tttttgacaa agaaccgtaa aaactaagcg ataccatgtt
       61 tttattttat atttgtattt gccaatttaa ctagcttggt tgttgatcca aagtattaat
      121 gcctggatac caatctttat atccttgata tctatctact tctagcagtt tttcagcatt
      181 ttttactctt tctggcgtag gaggctcaac accttcaaga cgatatctga tacccatttc
      241 atgatactta tgaacgccca ttgtatgata aggtaatact tcaaattttt cgacattatg
      301 aagattttcc ttaacatact cgcccaattg tttcaagtat tcatcattat ccgtgatgcc
      361 tgggataagg acatgacgaa tccaaacagg tttccctatg ctagaaagat attcgcacat
      421 atccaaaata ttatcatttg ggaatccggt taatctctta tgttcgtccg aattaatatg
      481 cttgatatca actaatagaa tatcggtata gtccattaac tcattgaact tactaaacca
      541 aggttctctt cttgtaaatg gctgtccaca agtgtccaaa caagtgctaa taccttcttc
      601 cttgcacatc ttgaataact ctagtgcaaa atctatttgc aatagaattt caccacctga
      661 taaagttatt ccacctttgt cgccccaaaa gctacgataa ggaagtgcct tatttagaac
      721 ttcttcagtc gtcatttgtg aacctaccct tgttttccaa gtgtccggat tgtgacagaa
      781 cttacacctc atatggcaac cttgcataaa aacgacaaaa cgtatacctg gtccatcgac
      841 agccccaaaa gtttccaaag aatgtacata gcctacgaca tgaccatctt tagttggaac
      901 tggattcaat aaaggattag cactcattct ctccacctac tctcttctaa atattacatt
      961 gaagtatgga aggttcttgc aataacgtca tcttgttgtt ctttagttag atcagcaaag
     1021 tatacacagt aacctgaaac acgaattgtt aatgttggat acttctctgg atgtgcttga
     1081 gcatctaata aagtatctct attgaataca ttaatattca aatgatgacc atcttttctc
     1141 atatagcctc cgatcatgtt taccaatgta tcttcttgca tcttttcatc atgacccaat
     1201 gttgttggtg ttacagcaaa tgtattagaa ataccatctg tagcatactt gtatggtaat
     1261 ttagcaacag ataataatga tgctaaagca cctgttgtat ctgctccgta tgctgggtta
     1321 gcacctggtg caaatggttc acccttttga cgaccatttg gagtagtacc tgtgttctta
     1381 ccataaacaa catttgaagt aattgtaaga acggatgttg ttaacttagc tccacgatat
     1441 agatgatgct tgctcatctt agtaaatagt tccttaacaa gccatgcagc aatatcgtca
     1501 gcacgatcat catcgttacc ataacgaggg aagtcatgat ctgctttaaa gtcaacagca
     1561 ataccatctt cgtcacgaat tactttaaca tgtccgtact tgatagctga aattgagtca
     1621 gcagcaacag acaatccaga aatacctgta gcaaagctac gatctactaa tgtatctttc
     1681 aatgccattt gtgctgattc atagtaatac ttgtcatgca tgtaatgaat tgtgttcaaa
     1741 gcatttacat aaatatcagc aatccattcc atcatgtcat cgaatttctt catgaattca
     1801 tcgtagtcaa tgtattctga gctaattggt tcgtatgcag gacctacttg ttgttttgta
     1861 agttcatcct taccaccatt gatagcatac aatacagcct tagctagatt tgcacgagct
     1921 ccgaagaatt gcataccatt tgcaattggt tgtgcagata cacagcaagc aatgccatag
     1981 tagtctgttc cccatgtctt catcattaag tcatcgtttt cgtattgaat tgtagaactt
     2041 gcaatagata cacttgcagc atagtgctta aatgattcag gcaaacgatc agaccaaagt
     2101 aatgtgatat ttggttctgg tgcggcaccc atattatgca atgtatttaa gaaacgatat
     2161 gatgtcttag ttacatggtg acgtccatct aagcccatac cagcaattga taatgttgcc
     2221 cagatagggt tacctgagaa tagtgagttg taagctggtg tacgaatgaa gcataccatt
     2281 ctcaatttca ttgtgaattg gtcaattaat tcttgtgctt ctttttcagt taaaatacca
     2341 cgcttcatat cacgttcaat gtaaatatcg atgaatgaat cgatacgacc aatagacata
     2401 gcagcaccat tttgttgttt aatagatgct aaatatccaa agtataacca ttgaattgct
     2461 tctttagctg ttgtagctgg tttggaaatg tcatagccat aagactcagc cataatcttc
     2521 atatcattta aggctttgat ttggtcagaa atctcttcac gcatttggat tatgtcgtta
     2581 gtcatctcac catcgccaca attttccaaa tcttttttct tttcttcgat taaacgatca
     2641 ataccataca atgcaacacg tgggaagtca ccaatcaaac ggccacgacc atatgcatct
     2701 ggtagtccag tgataatctt attgtggcga gctttcatga tttcgcttgt gtatgcatca
     2761 aatacacctt ggttatgcgt cttagataga ttttcaaata cgaagtctgt ttctggatca
     2821 atcttgaatc cataagcttc caaagcacct ttagccattc tgataccacc atatggcata
     2881 agtgcacgtt tcataggttt atctgtttgt aaaccaacaa tcttttctaa tgacttgtct
     2941 aaataacctg gtttatgaga tgtaattgtt gatactactt ttgtatcagc ttcaagaggt
     3001 ccaccatgtg cacgttcttt taattttaag tcgattaatt tatcgtttaa cttcgttgta
     3061 gcttcggttg gtccttcaag gaaagattga tctccatcat atggagtgaa gttatcttga
     3121 ataaattcac gaatatcaat ttcttctttc cattcttttc ctctaaatcc ttcccaagca
     3181 tcttttacat caacgttttg catttgagtc ttttcaacca tgctcaaagc ctcctttgtg
     3241 atttttaaga aaacgttttc tacactgtta atactacacc ttagttaaat taaattcaat
     3301 gaaaaatcgt tcacaatgtt ttttattttc aaaaaatctt ataaacgcta tcatttattt
     3361 taatcattat aaatgttaat atgacaatac ttttgacaac agtttataaa ctttaaaaat
     3421 gtgatttatg tatcaaaata taaatttcat taattttatt ttagaatttc ttaaaattac
     3481 aaaaaaagac cctatatcca aaaaatttag gtatagagtc tttaaaagta tttatactga
     3541 tgcccctaaa ataaaggtaa ctaacgtcgt taacaatcca gttataacat ttcgtaaaat
     3601 tgttttgcta gttgaaactt cggagttgtg tgtacaaact atcgcattaa ttgataatga
     3661 ggcaatcatc gcaattaatg taaataccac gcgatgctct ggtcttgcta ggatcatcgt
     3721 caataatgga atcattgccc caattataaa tgaacagaaa gaactggctg cagcatgaaa
     3781 cggcattaag atatcattct tttgcaattt tatcggacaa ttttcacagt catcattttc
     3841 caactgactt tgtctttcca ttgttttttc ttgaatatct ctttgagtgc ttacagatat
     3901 ccattctcca ccagccattg aacaagctcc agctagcatg ccagataagc cactgattaa
     3961 aagggtctta ctatcaagat tagccccagc agcacctaac acgattccag atactgaaat
     4021 aattccatca ttggcaccta aaatactagc tctgataaca ttcaaactat cccagaaaaa
     4081 attagttttt actcttaata catgcataac tttcactttg tacacttcct cacgaatttc
     4141 gcaaaaatat cattttttat atgtactgtc aaatatatgt aaaccgcttt atttacgatt
     4201 aaattataca ttatattagg a
//
