LOCUS       c00008_JACJGC0..        1779 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  JACJGC010000017.1 Bacillus subtilis strain RO-FF-1
            NODE_18_length_1779_cov_119.35_ID_6692, whole genome shotgun
            sequence.
ACCESSION   JACJGC010000017
VERSION     JACJGC010000017
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.0.0
            Run date     :: 2022-06-21 01:02:59
            Original ID  :: JACJGC010000017.1
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 1779
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..1779
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1:1777](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2..1777
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     cand_cluster    1..1779
                     /SMILES="NC(CCC(=O)O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..1779
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX))"
                     /tool="antismash"
     aSDomain        2..1111
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg8_1_AMP-binding.1"
                     /evalue="9.20E-104"
                     /label="ctg8_1_AMP-binding.1"
                     /locus_tag="ctg8_1"
                     /protein_end="370"
                     /protein_start="0"
                     /score="339.0"
                     /specificity="consensus: glu"
                     /tool="antismash"
                     /translation="RLAAHLAARGVTNESIVGILSERSPDMLIAVLAVLKAGGAYLPLD
                     PAYPKERLSYMLKDSGASLLLTQPGCSAPSFSGETLEVDMTSLDREEVKRYVSASVSDG
                     SLAYVIYTSGSTGQPKGVAVEHRQAVSFLTGMQHQFPLSEDDIVMVKTSFSFDASVWQL
                     FWWALSGASAYLLPPGWEKDSALIVKAIHQEKVTTVHFIPAMLNSFLDQAEMLALGDGT
                     NLKRVFAGGEPLAPRTAARFASVLPQVSLIHGYGPTEATVDAAFYVLDPERERDRLRIP
                     IGKPVPGARLYVLDPHLAVQPSGVAGELYIAGAGVARGYLNRPALTEERFLEDPFYPGE
                     RMYKTGDVARWLPDGNIEFLGRLDDQVKIR"
     aSModule        2..1624
                     /complete
                     /domains="nrpspksdomains_ctg8_1_AMP-binding.1"
                     /domains="nrpspksdomains_ctg8_1_PCP.1"
                     /locus_tags="ctg8_1"
                     /monomer_pairings="glu -> glu"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS             2..1777
                     /NRPS_PKS="Domain: AMP-binding (0-370). E-value: 9.2e-104.
                     Score: 339.0. Matches aSDomain:
                     nrpspksdomains_ctg8_1_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (472-541). E-value: 3.3e-26. Score:
                     83.1. Matches aSDomain: nrpspksdomains_ctg8_1_PCP.1"
                     /NRPS_PKS="type: NRPS-like protein"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     336.4; E-value: 3.9e-102)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg8_1"
                     /sec_met_domain="Condensation (E-value: 2.2e-11, bitscore:
                     29.5, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 8.5e-121, bitscore:
                     389.4, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 3.5e-18, bitscore:
                     52.2, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="RLAAHLAARGVTNESIVGILSERSPDMLIAVLAVLKAGGAYLPLD
                     PAYPKERLSYMLKDSGASLLLTQPGCSAPSFSGETLEVDMTSLDREEVKRYVSASVSDG
                     SLAYVIYTSGSTGQPKGVAVEHRQAVSFLTGMQHQFPLSEDDIVMVKTSFSFDASVWQL
                     FWWALSGASAYLLPPGWEKDSALIVKAIHQEKVTTVHFIPAMLNSFLDQAEMLALGDGT
                     NLKRVFAGGEPLAPRTAARFASVLPQVSLIHGYGPTEATVDAAFYVLDPERERDRLRIP
                     IGKPVPGARLYVLDPHLAVQPSGVAGELYIAGAGVARGYLNRPALTEERFLEDPFYPGE
                     RMYKTGDVARWLPDGNIEFLGRLDDQVKIRGYRIEPGEIEAALRSIEGVREAAVTVRTD
                     SGEPELCAYVEGLQRNEVRAQLESLLPGYMVPAYMIEMEQWPVTPSGKLDRNALPAPGG
                     AADAETYTAPRNVTEMKLSQLWEDVLKNGPVGIHDNFFDRGGHSLKATALVSRIAKEFD
                     VQVPLKDVFAHPTVEGLATVIREGTDSPYEAIKPAEKQETYPVSSAQKRIYVLQQLEDG
                     GTGYNMPAVLELEGKL"
     CDS_motif       95..136
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg8_1_0001"
                     /evalue="3.40E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="ctg8_1"
                     /protein_end="45"
                     /protein_start="31"
                     /score="20.1"
                     /tool="antismash"
                     /translation="LAVLKAGGAYLPLD"
     CDS_motif       320..379
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg8_1_0002"
                     /evalue="2.30E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="ctg8_1"
                     /protein_end="126"
                     /protein_start="106"
                     /score="35.3"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGQPKGVAVEH"
     CDS_motif       905..994
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg8_1_0003"
                     /evalue="3.30E-18"
                     /label="NRPS-A_a6"
                     /locus_tag="ctg8_1"
                     /protein_end="331"
                     /protein_start="301"
                     /score="57.7"
                     /tool="antismash"
                     /translation="PSGVAGELYIAGAGVARGYLNRPALTEERF"
     CDS_motif       1076..1141
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg8_1_0004"
                     /evalue="2.80E-13"
                     /label="NRPS-A_a8"
                     /locus_tag="ctg8_1"
                     /protein_end="380"
                     /protein_start="358"
                     /score="41.6"
                     /tool="antismash"
                     /translation="FLGRLDDQVKIRGYRIEPGEIE"
     aSDomain        1418..1624
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg8_1_PCP.1"
                     /evalue="3.30E-26"
                     /label="ctg8_1_PCP.1"
                     /locus_tag="ctg8_1"
                     /protein_end="541"
                     /protein_start="472"
                     /score="83.1"
                     /tool="antismash"
                     /translation="EMKLSQLWEDVLKNGPVGIHDNFFDRGGHSLKATALVSRIAKEFD
                     VQVPLKDVFAHPTVEGLATVIREG"
ORIGIN
        1 ccggctggct gcgcaccttg cggcacgcgg cgtcacaaac gaaagtatcg tcggtatcct
       61 gtctgaacgc tcacctgaca tgctgatcgc tgtccttgct gtattgaagg ccggaggggc
      121 gtatttgccg cttgatcctg cgtatccaaa ggagcggctg agctacatgc tgaaagacag
      181 cggggcatca ctcttgctga cacagccggg atgctccgca ccgagctttt ctggagagac
      241 gcttgaagtt gacatgacat ctcttgatcg tgaagaagta aaacgttatg tgtctgcttc
      301 tgttagcgac ggatctctcg cctatgtcat ctatacatcg ggctctaccg ggcagccgaa
      361 aggagtggcc gttgagcacc gccaggctgt ttcttttctg accggcatgc agcaccagtt
      421 tccgctttca gaagatgaca tcgtcatggt gaaaacctct ttttcctttg atgcgtctgt
      481 gtggcagctg ttttggtggg ctctttccgg tgcttcggcg tatctgcttc cgcccggctg
      541 ggaaaaagac tccgcattga tcgtaaaagc cattcatcag gaaaaagtga caacggttca
      601 ttttattccg gctatgctga acagctttct cgatcaagcg gaaatgttag cgcttggtga
      661 cgggacaaac ctaaagcgtg tattcgccgg aggtgaaccg cttgcgccgc gtacggcagc
      721 ccgttttgct tctgtattgc cgcaggtatc attaatccat ggctacgggc caacagaagc
      781 aacggtagac gcggcatttt acgtgttaga tccagaacgg gaaagggatc gtttgcggat
      841 tccgattggg aagcccgtac ctggtgcgcg tttgtatgtt ttagatccgc atttagccgt
      901 acagccttcc ggtgtcgccg gcgaactata cattgccgga gcgggtgttg ccagaggtta
      961 tttgaatcgg ccggcattaa cggaggagcg ttttcttgag gacccgtttt acccgggtga
     1021 acgcatgtac aaaaccgggg atgtggcgcg ctggctccct gacgggaata ttgaattcct
     1081 gggacggttg gatgatcagg tgaaaattcg cggctaccgg attgaaccgg gagaaattga
     1141 agcggcacta agaagtatag aaggtgtaag agaggcggcc gttaccgtac ggacagacag
     1201 cggcgagccg gaattatgcg cttatgtaga agggctccaa agaaacgaag tccgagcgca
     1261 gcttgagagc ctgttgccgg gctacatggt cccagcctat atgatagaaa tggaacagtg
     1321 gccggttaca ccaagcggaa agctcgaccg aaacgccctg ccagctcctg gcggagcggc
     1381 agatgcggag acctacacgg cgccgagaaa cgtgactgag atgaagcttt ctcagctgtg
     1441 ggaggacgtg ctgaaaaatg gccctgtcgg gattcacgac aacttttttg accgcggggg
     1501 acattcctta aaagcaacag cgcttgtctc acggatcgcc aaagaattcg atgtacaggt
     1561 gccgctaaaa gatgtgtttg cccatccgac ggtagaagga ctggctacag ttatccgtga
     1621 aggaacggac agtccatatg aagcgattaa gcccgctgaa aaacaggaaa cctatccggt
     1681 ttcctctgcc caaaagcgga tttatgtcct tcagcagctg gaagacggag ggacaggcta
     1741 caatatgccg gctgtgttag agctcgaagg gaagctcaa
//
