LOCUS       c00004_JADNEF0..        4964 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  JADNEF010000103.1 Ligilactobacillus salivarius strain D6t1_180914_C6
            NODE_103_length_4964_cov_49.2034, whole genome shotgun sequence.
ACCESSION   JADNEF010000103
VERSION     JADNEF010000103
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2022-06-21 20:10:07
            Original ID  :: JADNEF010000103.1
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4964
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4964
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1616:4747]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1617..4747
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4964
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4964
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             93..395
                     /locus_tag="ctg4_1"
                     /transl_table=11
                     /translation="MNKIIISKLNNDENKIEWRISNSETGHYLNISISRALEDAMKKKR
                     NLSFNRFESEQINNLSHLVTNIQEDYVLNIDKSNISSSYLPLKGIDALSYMKTVE"
     CDS             653..1531
                     /locus_tag="ctg4_2"
                     /transl_table=11
                     /translation="MYYNAALVLEGGAMRGLYTSGVLDTFLEHDIQFDCVIGVSAGSLN
                     GANFVSKQYQRTYNVNVKYRKDHDYISLARPFKHESIINLDFLFDDHGLDWHNFDNKAY
                     LKSETDFIVVATSLESGKAVTLRNPQPGQDLIDALEASSSMPFISKPHNTSKGLCLDGG
                     IANSIPFDIAQKMGFQKIVVVRTRPRDYRKKPTSFALKRLYSNSFKEYPEFVKTAIRRP
                     EMYNHSVEVLNKLEHNDEIFCLAPKDPVKVGRLEHNTKKMTELYNIGRNDAESNLEAML
                     NYLQKSEPLYD"
     CDS             complement(1617..2453)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 70.1;
                     E-value: 7.7e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg4_3"
                     /sec_met_domain="Radical_SAM (E-value: 5.5e-34, bitscore:
                     106.1, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSANPLLNPVPTKDGHVVGYVHSLETFGAVDGPGIRFVAFMQGCH
                     MRCKFCHNPDTWKTRVGSQMTTEEVLNKALPYRSFWGDKGGITLSGGEILLQIDFALEL
                     FKMCKEEGISTCLDTCGQPFTRREPWFSKFNELMDYTDILLVDIKHINSDEHKRLTGFP
                     NDNILDMCEYLSSIGKPVWIRHVLIPGITDNDEYLKQLGEYVKENLHNVEKFEVLPYHT
                     MGVHKYQEMGIRYRLEGVEPPTPERVKNAEKLLEVDRYQGYKDWYPGINTLDQQPS"
     CDS             complement(2480..4747)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg4_4"
                     /sec_met_domain="Gly_radical (E-value: 1.3e-41, bitscore:
                     129.7, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.5e-262, bitscore:
                     860.3, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVEKTQMQNVDVKDAWEGFRGKEWKEEIDIREFIQDNFTPYDGDQ
                     SFLEGPTEATTKLNNKLIDLKLKERAHGGPLEADTKVVSTITSHKPGYLDKSLEKIVGL
                     QTDKPMKRALMPYGGIRMAKGALEAYGFKIDPETDFVFENLSKTHNQGVFDAYTSEIMK
                     ARHNKIITGLPDAYGRGRLIGDFPRIALYGIDRLIEGKKKDLENCGDGEMTNDVIQMRE
                     EISDQIKALNDMKIMAESYGYDISKPATTAKEAIQWLYFGYLASIKQQNGAAMSIGRID
                     SFIDIYIERDMKRGILTEKEAQELIDQFTMKLRMVCFIRTPAYNSLFSGNPIWATLSIA
                     GMGLDGRHHVTKTSYRFLNTLHNMGAAPEPNITLLWSDRLPESFKHYAASVSIASSTIQ
                     YENDDLMMKTWGTDYYGIACCVSAQPIANGMQFFGARANLAKAVLYAINGGKDELTKQQ
                     VGPAYEPISSEYIDYDEFMKKFDDMMEWIADIYVNALNTIHYMHDKYYYESAQMALKDT
                     LVDRSFATGISGLSVAADSISAIKYGHVKVIRDEDGIAVDFKADHDFPRYGNDDDRVDD
                     IAAWLVKELFTKMSKHHLYRGAKLTTSVLTITSNVVYGKNTGTTPNGRQKGEPFAPGAN
                     PAYGADTTGALASLLSVAKLPYKYATDGISNTFAVTPTTLGHDEKMQEDTLVNMIGGYM
                     RKDGHHLNINVFNRDTLLDAQAHPEKYPTLTIRVSGYCVYFADLTKEQQDDVIARTFHA
                     SM"
ORIGIN
        1 taattttacc cttgctttta ttcgtaaatg agagtagagt tatacttgta agttaaggaa
       61 agcatttaca tttattaagg tggtaattat atatgaataa aattattatt agcaagttaa
      121 acaatgacga aaataagatt gaatggcgta tttctaacag tgaaactgga cattacttaa
      181 atatttcaat ttcccgtgca ttagaagatg ctatgaagaa aaagcgtaat ttatccttta
      241 accgctttga atctgaacaa attaacaatt tgtctcactt agttacaaat attcaagaag
      301 actacgttct taatattgat aaatcaaata ttagttctag ttatttacca ttaaagggta
      361 tcgacgcatt atcatatatg aaaaccgtcg aataagtgat tataagcatt aaattaagtt
      421 gttattttaa tacttatctt ggatatactt aagtattctt aattgattaa agatttatct
      481 gtcaacagcc tctattttga tgatagaggt tgtttttttg ttaaatattt tacagttgca
      541 ttgtttttaa ctaattaaat atttaaattc tcttattatt cctttgtatt cttgctatct
      601 ctacttttta ggtttatatt aattctagtt atttttcgaa aggaatggtt acatgtatta
      661 caatgcagct ctagttttag aaggtggtgc aatgcgtggc ctctatactt ctggcgttct
      721 agatacattt ttggaacatg atattcaatt tgattgtgtt attggagttt ctgcagggtc
      781 cttaaatggt gctaatttcg tttcaaaaca atatcaaaga acttataatg taaatgttaa
      841 ataccgtaaa gatcatgact atatctcttt agcacgccct tttaagcatg aaagtattat
      901 taacttagat ttcttatttg atgatcatgg attagattgg cataatttcg ataataaagc
      961 ctatttaaag tctgaaactg attttattgt tgtagctact tctctagaaa gcggaaaagc
     1021 tgtgacgcta cgtaatcccc aacctggtca agatttaatt gatgccttag aagcttcttc
     1081 atctatgcct ttcatctcaa agccacataa tacttctaaa ggactttgtc tagatggtgg
     1141 tattgctaac tctatccctt ttgatatcgc tcaaaaaatg ggttttcaaa aaattgtagt
     1201 tgttcgtact cgcccgcgtg attatcgcaa aaagcctact agttttgctt taaaacgtct
     1261 ctacagtaat agtttcaagg aatacccaga attcgttaaa acggctatca gacgccctga
     1321 aatgtataat cattctgttg aagttttaaa taaacttgaa cacaacgacg aaattttttg
     1381 tttagcaccc aaggatccag ttaaagttgg tagacttgaa cacaatacta aaaagatgac
     1441 tgaactctat aatataggta gaaatgatgc tgaaagtaat cttgaagcaa tgcttaacta
     1501 tcttcaaaaa agtgaacccc tatatgacta aaaatacatt ttaaaaccaa aaaacatggt
     1561 atctacttag caaaactaag cgataccatg tttttatttt atatttgcca atttagctag
     1621 cttggttgtt gatccaaagt attaatgcct ggataccaat ctttatatcc ttgatatcta
     1681 tctacttcta gcagtttttc agcatttttt actctttctg gcgtaggagg ctcaacacct
     1741 tcaagacgat atctgatacc catttcctga tacttatgaa cgcccattgt atgataaggt
     1801 aatacttcaa atttttcgac attatgaaga ttttccttaa catactcgcc caattgtttc
     1861 aagtattcat cattatccgt gatgcctggg ataaggacat gacgaatcca aacaggtttc
     1921 cctatactag aaagatattc gcacatatcc aaaatattat catttgggaa tccggttaat
     1981 ctcttatgtt cgtccgaatt aatatgcttg atatcaacta ataaaatgtc ggtatagtcc
     2041 attaactcat tgaacttact aaaccaaggt tctcttcttg taaatggctg tccacaagtg
     2101 tccaaacaag tgctaatacc ttcttccttg cacatcttga ataactctag tgcaaaatct
     2161 atttgcaata gaatttcacc acctgataaa gttattccac ctttgtcgcc ccaaaagctg
     2221 cgataaggaa gtgccttgtt tagaacttct tcagtcgtca tttgtgaacc tacccttgtt
     2281 ttccaagtgt ccggattgtg acagaactta cacctcatat ggcaaccttg cataaaagcg
     2341 acaaaacgta tacctggtcc atcaacagct ccaaaagttt ccaaagagtg tacatatcct
     2401 acgacatgac cgtctttagt tggaactgga ttcaataaag gattagcact cattctctcc
     2461 acctactctc ttctaattat tacattgaag catggaaggt tcttgcaata acgtcatctt
     2521 gttgttcttt agttaaatca gcaaagtata cacagtaacc tgaaacacga attgttaatg
     2581 ttggatactt ctctggatgt gcttgagcat ctaataaagt atctctgttg aatacattaa
     2641 tattcaaatg atgaccatct tttctcatat agcctccgat catgtttacc aatgtatctt
     2701 cttgcatctt ttcatcatga cccaatgttg ttggtgttac agcaaatgta ttagaaatac
     2761 catctgtagc atacttgtat ggtaatttag caacggataa tagtgatgct aaagcacctg
     2821 ttgtatctgc tccgtatgct gggttagcac ctggtgcaaa tggttcaccc ttttgacgac
     2881 catttggagt agtacctgtg ttcttaccat aaacaacatt tgaagtaatt gtaagaacgg
     2941 atgttgttaa cttagctcca cgatatagat gatgcttgct catcttagta aatagttcct
     3001 taacaagcca tgcagcaata tcgtcaacac gatcatcatc gttaccataa cgagggaagt
     3061 catgatctgc cttgaagtca acagcaatac catcttcgtc acgaattact ttaacatgtc
     3121 cgtacttgat agctgaaatt gagtcagcag caacagacaa tccagagata cctgtagcaa
     3181 agctacgatc taccaatgta tctttcaatg ccatttgtgc tgattcatag taatacttgt
     3241 catgcatgta atgaattgtg ttcaaagcat ttacgtaaat atcagcaatc cattccatca
     3301 tgtcatcgaa tttcttcatg aattcatcgt agtcaatgta ttctgagcta attggttcgt
     3361 atgcaggacc tacttgttgt tttgtaagtt catccttacc accattgata gcatacaata
     3421 cagccttagc tagatttgca cgagctccga agaattgcat accatttgca attggttgtg
     3481 cagatacaca gcaagcaatg ccatagtagt ctgttcccca tgtcttcatc attaagtcat
     3541 cgttttcgta ttgaattgta gaacttgcaa tagatacact tgcagcatag tgcttaaatg
     3601 attcaggcaa acgatcagac caaagtaatg taatatttgg ttctggtgcg gcacccatat
     3661 tatgcaatgt atttaagaaa cgatatgatg tcttagttac atggtgacgt ccatctaagc
     3721 ccataccagc aattgataat gttgcccaga tagggttacc tgagaatagt gagttgtaag
     3781 ctggtgtacg aatgaagcat accattctca atttcattgt gaattggtca attaattctt
     3841 gtgcttcttt ttcagttaaa ataccacgct tcatatcacg ttcaatgtaa atatcgatga
     3901 atgaatcgat acgaccaata gacatagcag caccattttg ttgcttgata gatgctaagt
     3961 aaccaaagta tagccattga attgcttctt tagctgttgt agctggttta gaaatgtcat
     4021 agccatagga ttcagccata atcttcatat cgtttaaggc tttgatttgg tcagaaattt
     4081 cttcacgcat ttggattacg tcgttagtca tttcaccatc gccgcaattt tccaaatcct
     4141 tcttctttcc ttcgatcaaa cgatcaatac cgtataatgc aatacgtggg aagtctccaa
     4201 tcaaacggcc acgaccatat gcatctggta gtccagtgat aatcttgttg tggcgagctt
     4261 tcatgatttc gcttgtgtat gcatcaaata caccttggtt atgcgtctta gatagatttt
     4321 caaatacgaa gtctgtttct ggatcaatct tgaatccata agcttccaaa gcacctttag
     4381 ccattctgat accaccatat ggcataagtg cacgtttcat aggtttatct gtttgtaaac
     4441 caacaatctt ttctaatgac ttgtctaaat aacctggttt atgagatgta attgttgata
     4501 ctacttttgt atcagcttca agaggtccac catgtgcacg ttcttttaat tttaagtcga
     4561 ttaatttatt gtttaacttc gttgtagctt cggttggtcc ttcaaggaaa gattgatctc
     4621 catcatatgg agtgaagtta tcttgaataa attcacgaat atcaatttct tctttccatt
     4681 cttttcctct aaatccttcc caagcatctt ttacatcaac gttttgcatt tgagtctttt
     4741 caaccatact caaagcctcc tttgtgattt ttaagaaaac gttttctaca ctgttaatac
     4801 tacaccttag ttaaattaaa ttcaatgaaa aatcgttcac aatgtttttt attttcaaaa
     4861 aatcttataa acgctatcat ttattttaat cattataaat gttaatatga caatattttt
     4921 gacaacagtt tataaacttt aaaaatgtga tttatgtatc aaaa
//
