LOCUS       c00021_JADMRB0..        7127 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  JADMRB010000029.1 Ligilactobacillus salivarius strain
            1001287B_170213_E2 NODE_29_length_7127_cov_49.4908, whole genome
            shotgun sequence.
ACCESSION   JADMRB010000029
VERSION     JADMRB010000029
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2022-06-21 20:12:53
            Original ID  :: JADMRB010000029.1
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7127
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..225
                     /locus_tag="ctg21_1"
                     /transl_table=11
                     /translation="GGQKFIPETLEKIRQLHQIKEERNLNFDIEVDGGINEETVKLCRE
                     AGATVAVAGSYVFNSGNETHAERINKLRN"
     protocluster    1..7127
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2850:5981]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2851..5981
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7127
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7127
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             244..819
                     /locus_tag="ctg21_2"
                     /transl_table=11
                     /translation="VISGIFYFYILMKAWGKMTIHDSNVREKLLQLGSQKRYNFTAEYA
                     RCGYKVTYRRGLDRDKLAPTIMFKNVKINNNLVTDHLWFNYTKGFAELGKLIVGDVISF
                     NARVASYEKLGHKIDYKLERPTKVKLVPHKNGKDALPIPNTTKGKNELLGYIMLENKKF
                     YQENNRPYYPWYVEKYKKFLDQRSSNTV"
     CDS             1327..1629
                     /locus_tag="ctg21_3"
                     /transl_table=11
                     /translation="MNKIIISKLNNDENKIEWRISNSETGHYLNISISRALEDAMKKKR
                     NLSFNRFESEQINNLSHLVTNIQEDYVLNIDESNISSSYLPLRGIDALSYMKTVE"
     CDS             1887..2765
                     /locus_tag="ctg21_4"
                     /transl_table=11
                     /translation="MYYNAALVLEGGAMRGLYTSGVLDTFLEHDIQFDCVIGVSAGSLN
                     GANFVSKQYQRTYNVNVKYRKDHDYISLARPFKHESIINLDFLFDDHGLDWHNFDNKAY
                     LKSETDFIVVATSLESGKAVMLRNPQPGQDLIDALEASSSMPFISKPHNTSKGLCLDGG
                     IANSIPFDIAQKMGFQKIVVVRTRPRDYRKKPTSFALKRLYSNSFKEYPGFVKTAIRRP
                     EMYNHSVEVLNKLEQNEEIFCLAPKDPVKVGRLEHNIKKMTELYNIGKNDAESNLEAML
                     NYLQKSEPLYD"
     CDS             complement(2851..3687)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 70.1;
                     E-value: 7.7e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg21_5"
                     /sec_met_domain="Radical_SAM (E-value: 9.6e-34, bitscore:
                     106.1, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSANPLLNPVPTKDGHVVGYVHSLETFGAVDGPGIRFVAFMQGCH
                     MRCKFCHNPDTWKTRVGSQMTTEEVLNKALPYRSFWGDKGGITLSGGEILLQIDFALEL
                     FKMCKEEGISTCLDTCGQPFTRREPWFSKFNELMDYTDILLVDIKHINSDEHKRLTGFP
                     NDNILDMCEYLSSIGKPVWIRHVLIPGITDNDEYLKQLGEYVKENLHNVEKFEVLPYHT
                     MGVHKYQEMGIRYRLEGVEPPTPERVKNAEKLLEVDRYQGYKDWYPGINTLDQQPS"
     CDS             complement(3714..5981)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg21_6"
                     /sec_met_domain="Gly_radical (E-value: 2.3e-41, bitscore:
                     129.7, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.7e-262, bitscore:
                     861.0, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVEKTQMQNVDVKDAWEGFRGKEWKEEIDIREFIQDNFTPYDGDQ
                     SFLEGPTEATTKLNDKLIDLKLKERAHGGPLEADTKVVSTITSHKPGYLDKSLEKIVGL
                     QTDKPMKRALMPYGGIRMAKGALEAYGFKIDPETDFVFENLSKTHNQGVFDAYTSEIMK
                     ARHNKIITGLPDAYGRGRLIGDFPRVALYGIDRLIEEKKKDLENCGDGEMTNDIIQMRE
                     EISDQIKALNDMKIMAESYGYDISKPATTAKEAIQWLYFGYLASIKQQNGAAMSIGRID
                     SFIDIYIERDMKRGILTEKEAQELIDQFTMKLRMVCFIRTPAYNSLFSGNPIWATLSIA
                     GMGLDGRHHVTKTSYRFLNTLHNMGAAPEPNITLLWSDRLPESFKHYAASVSIASSTIQ
                     YENDDLMMKTWGTDYYGIACCVSAQPIANGMQFFGARANLAKAVLYAINGGKDELTKQQ
                     VGPAYEPISSEYIDYDEFMKKFDDMMEWIADIYVNALNTIHYMHDKYYYESAQMALKDT
                     LVDRSFATGISGLSVAADSISAIKYGHVKVIRDEDGIAVDFKADHDFPRYGNDDDRVDD
                     IAAWLVKELFTKMSKHHLYRGAKLTTSVLTITSNVVYGKNTGTTPNGRQKGEPFAPGAN
                     PAYGADTTGALASLLSVAKLPYKYATDGISNTFAVTPTTLGHDEKMQEDTLVNMIGGYM
                     RKDGHHLNINVFNRDTLLDAQAHPEKYPTLTIRVSGYCVYFADLTKEQQDDVIARTFHA
                     SM"
     CDS             complement(6292..6876)
                     /locus_tag="ctg21_7"
                     /transl_table=11
                     /translation="VKVMHVLRVKTNFFWDSLNVIRASILGANDGIISVSGIVLGAAGA
                     NLDSKTLLISGLSGMLAGACSMAGGEWISVSTQRDIQEKTMERQSQLENDDCENCPIKL
                     QKNDILMPFHAAASSFCSFIIGAMIPLLTMILARPEHRVVFTLIAMIASLSINAIVCTH
                     NSEVSTSKTILRNVITGLLTTLVTFILGASV"
ORIGIN
        1 ggtggacaaa aattcatacc agaaacatta gaaaagattc gccaactaca tcaaataaaa
       61 gaagaacgaa atcttaattt tgatatagaa gttgatggtg gaattaacga agaaactgtt
      121 aagttatgtc gagaagctgg cgcaacagtt gcagttgccg ggtcatatgt ttttaattct
      181 ggtaatgaaa ctcatgctga aagaataaat aaattacgta attaatatat agaagatact
      241 actgtgatta gtggtatctt ctatttttat atattgatga aagcgtgggg aaaaatgact
      301 atacatgatt cgaatgttcg ggaaaaatta cttcaattag gaagtcaaaa acggtacaat
      361 tttacagctg aatatgcaag gtgtggttac aaagttacat ataggcgtgg attagataga
      421 gataaattag ctccaacaat tatgttcaaa aatgttaaga taaataacaa tctagttaca
      481 gaccatctgt ggttcaacta cacaaaaggc tttgctgagt tgggaaaact aattgttggg
      541 gatgttatta gttttaatgc aagagttgct agctatgaga aattaggaca taaaatagat
      601 tataaattag aacgtccaac aaaagttaaa ttagttcccc ataaaaatgg taaagatgct
      661 ttgcctatac ctaacactac aaaaggtaaa aatgagttgt tgggatacat tatgctagag
      721 aataaaaaat tttatcaaga aaataatcgt ccatattacc catggtatgt tgaaaagtat
      781 aaaaaattct tagatcaaag atcttctaac actgtttaat aatagataca ctttaaagtc
      841 tccaatttac tataaagatt agggggattt ttgtcatgat taattcttaa ttcatctttc
      901 taaaaaatgt atgtttttca taaagatgga tagagagaaa accaaacagt gtaatataat
      961 ctcattatag ctttattgat taattttgat gtttatcaaa aatcatattt tttcagaaag
     1021 ttaaaataac tttttatttt gttggaaatt aatttcctaa ttttgatttt aataatctgt
     1081 ctacgatctg ttgtttttaa agacaaattt gtattatata actgattcca ttcaataatc
     1141 ctttatcaaa acaattatat taacatacca atccttatat tatatggata ttcaactttt
     1201 taaataagta aaaacaatta tttacttata ttttcaattt tacccttgct tttattcgta
     1261 aataagagta gagttatact tgtaagttaa ggaaagcatt tacatttatt aaggtggtaa
     1321 ttatatatga ataaaattat tattagcaag ttaaacaatg acgaaaataa gattgaatgg
     1381 cgtatttcta acagtgaaac tggacattac ttaaatattt caatttcccg tgcattagaa
     1441 gatgctatga agaaaaagcg taatttatcc tttaaccgct ttgaatctga acaaattaac
     1501 aatttgtctc acttagttac aaacattcaa gaagactatg ttcttaatat tgatgaatcc
     1561 aatattagct ctagttattt accattaagg ggtatcgacg cattatcata tatgaaaacc
     1621 gtcgaataag tgattataag cattaaatta agttgttatt ttaatactta tcttggatat
     1681 atttaagtat tcttaattga ttaaagattt gtctgtcaac aacctctatt ttgatgatag
     1741 aggttgtttt tttgttaaat attttacagt tgcattgttt ttgactaatt aaatatttaa
     1801 attctcttat tattcctttg tattcttgct atctctactt tttaggttta tattaattct
     1861 agttattttt cgaaaggaat ggttacatgt attacaatgc agctctagtt ttagaaggtg
     1921 gtgcaatgcg tggcctctat acttctggcg ttctagatac ctttttggaa catgatattc
     1981 aatttgattg tgttattgga gtttctgcag ggtccctaaa tggtgctaat ttcgtttcaa
     2041 aacaatatca aagaacttat aatgtaaatg ttaaataccg taaagatcat gactatatct
     2101 ctttagcacg cccttttaag catgaaagta ttattaactt agatttctta tttgatgatc
     2161 atggattaga ttggcataat ttcgataata aagcttattt aaagtctgaa actgatttta
     2221 ttgttgtagc tacttctcta gaaagcggaa aagctgtgat gctacgtaat ccccaacctg
     2281 gccaagactt aattgatgct ttagaagctt cttcatctat gcctttcatc tcaaagccac
     2341 ataatacttc taaaggactt tgtctagatg gtggtattgc taactctatc ccttttgata
     2401 tcgctcaaaa aatgggtttt caaaaaattg tagttgttcg tactcgtccg cgtgattatc
     2461 gcaaaaagcc tactagtttt gctttaaaac gcctctacag taatagtttc aaggaatacc
     2521 caggattcgt taaaacggct atcagacgtc ctgaaatgta taaccattct gttgaagttt
     2581 taaataaact tgaacagaat gaagaaattt tctgtctagc acctaaagat ccagttaaag
     2641 ttggtagact tgaacacaat attaaaaaga tgactgaact ctataatata ggtaaaaatg
     2701 atgctgaaag taatcttgaa gcaatgctta actatcttca aaaaagtgaa cctctatatg
     2761 actaaaatac attttaaaag caaaaaacat gatatctacc tagtaaaaac taagcgatac
     2821 catgttttta ttttatattt gccaatttag ctagcttggt tgttgatcca aagtattaat
     2881 gcctggatac caatctttat atccttgata tctatctact tctagcagtt tttcagcatt
     2941 ttttactctt tctggcgtag gaggctcaac accttcaaga cgatatctga tacccatttc
     3001 ctgatactta tgaacgccca ttgtatgata aggtaatact tcaaattttt cgacattatg
     3061 aagattttcc ttaacatact cgcccaattg tttcaagtat tcatcattat ccgtgatgcc
     3121 tgggataagg acatgacgaa tccaaacagg tttccctatg ctagaaagat attcgcacat
     3181 atccaaaata ttatcatttg ggaatccggt taatctctta tgttcgtccg aattaatatg
     3241 cttgatatca actaataaaa tgtcggtata gtccattaac tcattgaact tactaaacca
     3301 aggttctctt cttgtaaatg gctgtccaca agtgtccaaa caagtgctaa taccttcttc
     3361 cttgcacatc ttgaataact ctagtgcaaa atctatttgc aatagaattt caccacctga
     3421 taaagttatt ccacctttgt cgccccaaaa gctgcgataa ggaagtgcct tgtttagaac
     3481 ttcttcagtc gtcatttgtg aacctaccct tgttttccaa gtgtccggat tgtgacagaa
     3541 cttacacctc atatggcaac cttgcataaa agcgacaaaa cgtatacctg gtccatcaac
     3601 agctccaaaa gtttccaaag agtgtacata tcctacgaca tgaccgtctt tagttggaac
     3661 tggattcaat aaaggattag cactcattct ctccacctac tctcttctaa ttattacatt
     3721 gaagcatgga aggttcttgc aataacgtca tcttgttgtt ctttagttaa atcagcaaag
     3781 tatacacagt aacctgaaac acgaattgtt aatgttggat acttctctgg atgtgcttga
     3841 gcatctaata aagtatctct gttgaataca ttaatattca aatgatgacc atcttttctc
     3901 atatagcctc cgatcatgtt taccaatgta tcttcttgca tcttttcatc atgacccaat
     3961 gttgttggtg ttacagcaaa tgtattagaa ataccatctg tagcatactt gtatggtaat
     4021 ttagcaacgg ataatagtga tgctaaagca cctgttgtat ctgctccgta tgctgggtta
     4081 gcacctggtg caaatggttc acccttttga cgaccatttg gagtagtacc tgtgttctta
     4141 ccataaacaa catttgaagt aattgtaaga acggatgttg ttaacttagc tccacgatat
     4201 agatgatgct tgctcatctt agtaaatagt tccttaacaa gccatgcagc aatatcgtca
     4261 acacgatcat catcgttacc ataacgaggg aagtcatgat ctgccttgaa gtcaacagca
     4321 ataccatctt cgtcacgaat tactttaaca tgtccgtact tgatagctga aattgagtca
     4381 gcagcaacag acaatccaga gatacctgta gcaaagctac gatctaccaa tgtatctttc
     4441 aatgccattt gtgctgattc atagtaatac ttgtcatgca tgtaatgaat tgtgttcaaa
     4501 gcatttacgt aaatatcagc aatccattcc atcatgtcat cgaatttctt catgaattca
     4561 tcgtagtcaa tgtattctga gctaattggt tcgtatgcag gacctacttg ttgttttgta
     4621 agttcatcct taccaccatt gatagcatac aatacagcct tagctagatt tgcacgagct
     4681 ccgaagaatt gcataccatt tgcaattggt tgtgcagata cacagcaagc aatgccatag
     4741 tagtctgttc cccatgtctt catcattaag tcatcgtttt cgtattgaat tgtagaactt
     4801 gcaatagata cacttgcagc atagtgctta aatgattcag gcaaacgatc agaccaaagt
     4861 aatgtaatat ttggttctgg tgcggcaccc atattatgca atgtatttaa gaaacgatat
     4921 gatgtcttag ttacatggtg acgtccatct aagcccatac cagcaattga taatgttgcc
     4981 cagatagggt tacctgagaa tagtgagtta taagctggtg tacgaatgaa gcataccatt
     5041 ctcaatttca ttgtgaattg gtcaattaat tcttgtgctt ctttttcagt taaaatacca
     5101 cgcttcatat cacgttcaat gtaaatatcg atgaatgaat cgatacgacc aatagacatt
     5161 gcagcaccat tttgttgttt aatagatgct aaatatccaa aatataacca ttgaattgct
     5221 tctttagctg ttgtagctgg tttggaaatg tcatagccat aagactcagc cataatcttc
     5281 atatcattta aggctttgat ttgatcagaa atctcttcac gcatttggat tatgtcgtta
     5341 gtcatctcac catcgccaca attttccaaa tcttttttct tttcttcgat taaacgatca
     5401 atgccataca atgcaacacg tgggaagtca ccaatcaaac ggccacgacc atatgcatct
     5461 ggtagtccag tgataatctt gttgtggcga gctttcatga tttcgcttgt gtatgcatca
     5521 aatacacctt ggttatgtgt cttagataga ttttcaaata cgaagtctgt ttctggatca
     5581 atcttaaatc cataagcttc caaagcacct ttagccattc tgataccacc atatggcata
     5641 agtgcacgtt tcataggttt atctgtttgt aaaccaacaa tcttttctaa tgacttgtct
     5701 aaataacctg gtttatgaga tgtaattgtt gatactactt ttgtatcagc ttcaagaggt
     5761 ccaccatgtg cacgttcttt taattttaag tcgattaatt tatcgtttaa cttcgttgta
     5821 gcttcggttg gtccttcaag gaaagattga tctccatcat atggagtgaa gttatcttga
     5881 ataaattcac gaatatcaat ttcttctttc cattcttttc ctctaaatcc ttcccaagca
     5941 tcttttacat caacgttttg catttgagtc ttttcaacca tgctcaaagc ctcctttgtg
     6001 atttttaaga aaacgttttc tacactgtta atactacacc ttagttaaat taaattcaat
     6061 gaaaaatcgt tcacaatgtt ttttattttc aaaaaatctt ataaacgcta tcatttattt
     6121 taatcattat aaatgttaat atgataatac ttttgacaac agtttataaa ctttaaaaat
     6181 gtgatttatg tatcaaaata taaatttcat taattttatt ttagaatttc ttaaaattac
     6241 aaaaaaagac cctatatcca aaaaatttag gtatagagtc tttaaaagta tttatactga
     6301 tgcccctaaa ataaaggtaa ctaacgtcgt taacaatcca gttataacat ttcgtaaaat
     6361 tgttttgcta gttgaaactt cggagttgtg tgtacaaact atcgcattaa ttgataatga
     6421 ggcaatcatc gcaattaatg taaataccac gcgatgttct ggccttgcta ggatcatcgt
     6481 caataatgga atcattgccc caattataaa tgaacagaaa gaactggctg cagcatgaaa
     6541 cggcattaag atatcattct tttgcaattt tatcggacaa ttttcacagt catcattttc
     6601 caactgactt tgtctttcca ttgttttttc ttgaatatct ctttgagtgc ttacagatat
     6661 ccattctcca ccagccattg aacaagctcc agctagcatg ccagataagc cactgattaa
     6721 aagggtctta ctatcaagat tagccccagc agcacctaac acgattccag atactgaaat
     6781 aattccatca ttggcaccta aaatactagc tctgataaca ttcaaactat cccagaaaaa
     6841 attagttttt actcttaata catgcataac tttcactttg tacacttcct cacgaatttc
     6901 gcaaaaatat cattttttat atgtactgtc aaatatatgt aaaccgcttt atttacgatt
     6961 aaattataca ttatattagg atttattact aatttagcaa aatttataat tatatacatg
     7021 tttcacaata atataaaaaa gcatactttt tgaagtgcct tacaattgtt agacaaagtc
     7081 tagagattgt gaggtgcttt tttcatatga ctaaatatac tcaaaaa
//
