GCA_015667455.1 - 6 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

JADPEB010000010 (original name was: JADPEB010000010.1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1.1	RiPP-like	1,328	20,170

JADPEB010000003 (original name was: JADPEB010000003.1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 32.1	lanthipeptide-class-i	37,844	62,070	streptin	RiPP:Lanthipeptide	90%
Region 32.2	NRPS-like,T1PKS,NRPS,lanthipeptide-class-ii	82,158	172,194	salivaricin 9	RiPP:Lanthipeptide	72%

JADPEB010000006 (original name was: JADPEB010000006.1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 36.1	RiPP-like	72,006	82,302

JADPEB010000007 (original name was: JADPEB010000007.1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 37.1	RiPP-like	1	7,725

JADPEB010000008 (original name was: JADPEB010000008.1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 38.1	T3PKS	63,543	104,709

Region	Type	From	To	Most similar known cluster	Similarity
Region 1.1	RiPP-like	1,328	20,170
Region 32.1	lanthipeptide-class-i	37,844	62,070	streptin	RiPP:Lanthipeptide	90%
Region 32.2	NRPS-like,T1PKS,NRPS,lanthipeptide-class-ii	82,158	172,194	salivaricin 9	RiPP:Lanthipeptide	72%
Region 36.1	RiPP-like	72,006	82,302
Region 37.1	RiPP-like	1	7,725
Region 38.1	T3PKS	63,543	104,709

Compact view

c00001_JADPEB0.. - Region 1 - RiPP-like

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Location: 1,328 - 20,170 nt. (total: 18,843 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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KnownClusterBlast

Similar known gene clusters

No matches found.

c00032_JADPEB0.. - Region 1 - lanthipeptide-class-i

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Location: 37,844 - 62,070 nt. (total: 24,227 nt) Show pHMM detection rules used

lanthipeptide-class-i: ((LANC_like and (Lant_dehydr_N or Lant_dehydr_C)) and not (YcaO or TIGR03882))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Lanthipeptides

KnownClusterBlast

Lanthipeptide predictions

Lanthipeptide(s) for ctg32_54 - Putative Class I

ctg32_51 leader / core peptide: MKNTIKDFDLDLKTTKNNSDEPL - VGDhaRYLCDhbPGDhaCWKLVCFDhbDhbDhbVK

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Similar known gene clusters

Lanthipeptides

Core peptide predictions

ctg32_51 - Class I

Cleavage pHMM score: 6.80

RODEO score: 22

Monoisotopic mass: 2440.3 Da

Molecular weight: 2441.9 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 2459.9 Da N = 1; 2477.9 Da N = 2; 2496.0 Da N = 3

c00032_JADPEB0.. - Region 2 - NRPS,NRPS-like,T1PKS,lanthipeptide-class-ii

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Location: 82,158 - 172,194 nt. (total: 90,037 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))
lanthipeptide-class-ii: cds(LANC_like and DUF4135)

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core biosynthetic genes

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NRPS/PKS domains

Lanthipeptides

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Lanthipeptide predictions

Lanthipeptide(s) for ctg32_131 - Putative Class II

ctg32_132 leader / core peptide: MKSTNNQSIAEIAAVNSLQEVSMEELDQIIGA - GNGVVLDhbLDhbHECNLADhbWDhbKKLKCC

Lanthipeptide(s) for ctg32_142 - Putative Class II

ctg32_143 leader / core peptide: MKNSKDILNNAIEEVSEKELMEVAGG - KRGDhbGWFADhbIDhbDDCPNDhaVFVCC

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Similar known gene clusters

Lanthipeptides

NRPS/PKS products

NRPS/PKS monomers

Core peptide predictions

ctg32_132 - Class II

Cleavage pHMM score: 5.20

RODEO score: 0

Monoisotopic mass: 2559.3 Da

Molecular weight: 2561.0 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 2579.1 Da N = 1

ctg32_143 - Class II

Cleavage pHMM score: 4.40

RODEO score: 0

Monoisotopic mass: 2347.1 Da

Molecular weight: 2348.7 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 2366.7 Da N = 1

Predicted core structure(s)

For candidate cluster 2, location 82157 - 172194:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (asp) + (X) + (pk) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 82157 - 130377:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (asp) + (X) + (pk) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 90378 - 140068:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (asp) + (X) + (pk) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg32_101

Search NORINE for peptide: strict or relaxed

AMP-binding (12..378): X

NRPSPredictor2: val, leu, ile, abu, iva

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DlLFFGaivK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 60% (weak)

AMP-binding (990..1376): X

NRPSPredictor2: orn, lys, arg

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: orn, lys, arg
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVsnnGSIcK
Nearest Stachelhaus code:: orn
Stachelhaus code match:: 60% (weak)

ctg32_102

PKS_AT (504..788): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 62.5%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 35.6
Methylmalonyl-CoA: 30.7
Propionyl-CoA: 16.0
Ethylmalonyl-CoA: 15.1
CHC-CoA: 11.1
trans-1,2-CPDA: 8.8
inactive: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0
Malonyl-CoA: -0.3

ctg32_103

Search NORINE for peptide: strict or relaxed

AMP-binding (471..823): X

NRPSPredictor2: hydrophobic-aliphatic

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DiEsiGTpVK
Nearest Stachelhaus code:: lys-b
Stachelhaus code match:: 60% (weak)

ctg32_105

Search NORINE for peptide: strict or relaxed

AMP-binding (19..400): asn

NRPSPredictor2: asn

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asn

Stachelhaus prediction details:

Stachelhaus sequence:: DATKVGEVGK
Nearest Stachelhaus code:: asn
Stachelhaus code match:: 100% (strong)

ctg32_106

Search NORINE for peptide: strict or relaxed

AMP-binding (217..613): asp

NRPSPredictor2: asp

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asp

Stachelhaus prediction details:

Stachelhaus sequence:: DfTKMGHiGK
Nearest Stachelhaus code:: asp
Stachelhaus code match:: 80% (moderate)

c00036_JADPEB0.. - Region 1 - RiPP-like

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Location: 72,006 - 82,302 nt. (total: 10,297 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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KnownClusterBlast

Similar known gene clusters

No matches found.

c00037_JADPEB0.. - Region 1 - RiPP-like

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Location: 1 - 7,725 nt. (total: 7,725 nt) Show pHMM detection rules used

Region on contig edge.

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

KnownClusterBlast

Similar known gene clusters

No matches found.

c00038_JADPEB0.. - Region 1 - T3PKS

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Location: 63,543 - 104,709 nt. (total: 41,167 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

KnownClusterBlast

Similar known gene clusters

No matches found.