GCA_020076235.1 - 6 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

JAIQWT010000015 (original name was: JAIQWT010000015.1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 6.1	RiPP-like	12,213	24,309

JAIQWT010000018 (original name was: JAIQWT010000018.1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 9.1	lanthipeptide-class-ii	24,577	45,174	salivaricin A	RiPP:Lanthipeptide	87%

JAIQWT010000001 (original name was: JAIQWT010000001.1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 11.1	T3PKS	100,050	141,216	coumermycin A1	Saccharide:Hybrid/tailoring + Other:Aminocoumarin	6%

JAIQWT010000002 (original name was: JAIQWT010000002.1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 22.1	RiPP-like	137,838	156,629

JAIQWT010000032 (original name was: JAIQWT010000032.1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 25.1	lanthipeptide-class-ii	1	6,777	streptococcin A-FF22	RiPP:Lanthipeptide	30%

JAIQWT010000006 (original name was: JAIQWT010000006.1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 40.1	transAT-PKS,NRPS	61,058	116,106	thermophilin 1277	RiPP:Lanthipeptide	10%

Region	Type	From	To	Most similar known cluster	Similarity
Region 6.1	RiPP-like	12,213	24,309
Region 9.1	lanthipeptide-class-ii	24,577	45,174	salivaricin A	RiPP:Lanthipeptide	87%
Region 11.1	T3PKS	100,050	141,216	coumermycin A1	Saccharide:Hybrid/tailoring + Other:Aminocoumarin	6%
Region 22.1	RiPP-like	137,838	156,629
Region 25.1	lanthipeptide-class-ii	1	6,777	streptococcin A-FF22	RiPP:Lanthipeptide	30%
Region 40.1	transAT-PKS,NRPS	61,058	116,106	thermophilin 1277	RiPP:Lanthipeptide	10%

Compact view

c00006_JAIQWT0.. - Region 1 - RiPP-like

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Location: 12,213 - 24,309 nt. (total: 12,097 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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KnownClusterBlast

Similar known gene clusters

No matches found.

c00009_JAIQWT0.. - Region 1 - lanthipeptide-class-ii

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Location: 24,577 - 45,174 nt. (total: 20,598 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Lanthipeptides

KnownClusterBlast

Lanthipeptide predictions

Lanthipeptide(s) for ctg9_28 - Putative Class II

ctg9_27 leader / core peptide: MKNSKDILNNAIEEVSEKELMEVAGG - KRGDhbGWFADhbIDhbDDCPNDhaVFVCC

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Similar known gene clusters

Lanthipeptides

Core peptide predictions

ctg9_27 - Class II

Cleavage pHMM score: 4.40

RODEO score: 0

Monoisotopic mass: 2347.1 Da

Molecular weight: 2348.7 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 2366.7 Da N = 1

c00011_JAIQWT0.. - Region 1 - T3PKS

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Location: 100,050 - 141,216 nt. (total: 41,167 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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KnownClusterBlast

Similar known gene clusters

c00022_JAIQWT0.. - Region 1 - RiPP-like

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Location: 137,838 - 156,629 nt. (total: 18,792 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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KnownClusterBlast

Similar known gene clusters

No matches found.

c00025_JAIQWT0.. - Region 1 - lanthipeptide-class-ii

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Location: 1 - 6,777 nt. (total: 6,777 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

Lanthipeptides

KnownClusterBlast

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Similar known gene clusters

Lanthipeptides

Core peptide predictions

No core peptides found.

c00040_JAIQWT0.. - Region 1 - NRPS,transAT-PKS

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Location: 61,058 - 116,106 nt. (total: 55,049 nt) Show pHMM detection rules used

transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))
NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 61057 - 116106:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - mal) + (cys) + (ohmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 61057 - 108208:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - mal) + (cys) + (ohmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 70535 - 116106:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - mal) + (cys) + (ohmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg40_91

PKS_AT (6..282): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 70.8%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 67.0
inactive: 35.5
Methoxymalonyl-CoA: 15.9
Methylmalonyl-CoA: 11.2
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ctg40_95

Search NORINE for peptide: strict or relaxed

AMP-binding (824..1226): cys

NRPSPredictor2: cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: cys
Small clusters prediction:: cys
Single AA prediction:: cys

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNLSLvWK
Nearest Stachelhaus code:: cys
Stachelhaus code match:: 90% (moderate)

ctg40_96

Search NORINE for peptide: strict or relaxed

AMP-binding (11..360): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: phe, trp
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: ipWTIldtlK
Nearest Stachelhaus code:: phe
Stachelhaus code match:: 40% (weak)