LOCUS       c00065_JAFFQQ0..        7964 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  JAFFQQ010000063.1 Lactiplantibacillus plantarum strain DY46
            DY46_contig_63, whole genome shotgun sequence.
ACCESSION   JAFFQQ010000063
VERSION     JAFFQQ010000063
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2022-06-21 04:11:13
            Original ID  :: JAFFQQ010000063.1
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7964
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7964
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2531:5648]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2532..5648
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7964
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7964
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(15..935)
                     /locus_tag="ctg65_1"
                     /transl_table=11
                     /translation="LKTINLGHSNIQASSVALGIMRMDALDVAQATKVIDTAVDLGINY
                     VDSADIYGGGQSSTIFGQALKQAHVSRDQLYIQSKGGIVPGKRYDFSKQHILAAVDGEL
                     QRLGVDYLDTFLLHRPDALMEPEEVAAAFDKLQTSGKVRHFGVSNFNPMQVDMLQAALN
                     QRLIINQLQFGIMHTGAIDFGLHTNMQDERSLNHDGEMIEYSRLHKMTIQAWSPYQYGN
                     FAGIFLDNPKFPELNNAMQALADEKQVTKSAIATAWILRHPAHFQVILGTMNPEHLAQN
                     AAGADVTLSRQEWYDIYFAAGNDLP"
     CDS             1978..2307
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) FeS_assembly_P"
                     /gene_functions="other (smcogs) SMCOG1048:Iron-sulfur
                     cluster assembly protein (Score: 66.9; E-value: 4.7e-21)"
                     /gene_kind="other"
                     /locus_tag="ctg65_2"
                     /sec_met_domain="FeS_assembly_P (E-value: 5.3e-22,
                     bitscore: 66.9, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEGRDTMTTTFKDRAFEALGHVIDPELGIDLVNLGLIYDAQLTAE
                     GTAEITMTLTIAGCPLTEWLAKDIHDELIKLPEVNNIEIEIVWEPAWNESMMSREARME
                     LGIHS"
     CDS             complement(2331..2516)
                     /locus_tag="ctg65_3"
                     /transl_table=11
                     /translation="MTKTPAIQSSTDGCVTGVFLLARRMHVLLAGISTVARKKGAASLN
                     QQRLSLIMPYPMTVSP"
     CDS             complement(2532..3353)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 74; E-value:
                     4.8e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg65_4"
                     /sec_met_domain="Radical_SAM (E-value: 2.7e-28, bitscore:
                     88.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MENKQVSTTQAAAKEPLIGYVHSIETFGSVDGPGIRYVAFLQGCH
                     MRCQYCHNPDTWKLNVGDQMTADEILEDAAKYRAFWGKTGGITVSGGESLVQIDFILDL
                     FEKAKAMNISTCLDTSGQPFTREQPFFDKFERLMKVTDISLVDIKHIDSAKHKQLTQYG
                     NENILDMIQYMAQHHDDMWIRHVLVPQRTDYDEDLKKLGDYIAKIPNDVVQKVEVLPYH
                     TLGVKKYHEMKIKYRLEGIESPTQDRVANAEKLLHTADYNGYKTWMPLPKL"
     CDS             complement(3390..5648)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg65_5"
                     /sec_met_domain="Gly_radical (E-value: 1.7e-40, bitscore:
                     126.9, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 2.8e-247, bitscore:
                     810.7, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MITSEKTTKPAAWKGFKGGHWQEEINIRDFIQNNFTQYNGDESFL
                     AGPTAATKTLNDKVLELKKQERAAGGVLDADTKVVATITSHGPGYIQKDLEKIVGLQTD
                     KPLKRAFMPFGGIRMADDALKSYGYTPDEENDKIFTEYRKTHNQGVFDVYTPDMRKARH
                     YKIITGLPDAYARGRLIPDLPRVAVYGIDRLMEDKANDFAHIGDGELTDDVIRLREEVQ
                     DQYRALADMKKMAASYGYDISKPATNAQEAIQWMYFAYLAAIKTQNGAAMSVGRIDTTM
                     DIFIQRDLDNGVLDESQAQELIDQFVMKLRMVRFIRTEEYNSLFSGDPIWATLSMCGLG
                     VDGQHHVTKTAFRILKTLDNMGAAPEPNITILWSDRLPEDFKRYATEVSIDSSTIQYEN
                     DDLMRVQWGTDYYGIACCVSAQPIADGIQYFGARANLAKAILYAINGGRDEIAGDQVGP
                     AYEPITSEYIDYDEFMKKLDKQMDWLADTYVNSLNAIHYMHDKYYYEAAQLALKNTDLD
                     RTFATGISGLSHAADSISAIKYGHVKVIRDERGIAVDFKADNDYPRYGNNDDRADDIAK
                     WLVKELYSKMNTHHLYRNAKLSTSVLTITSNVVYGKNTGTTPNGRQKGEPFSPGANPAY
                     GAEKSGALASLLSTAKLPYRYATDGISNTFGVTPNTLGHDLESRKDTLVNMLDGYMKND
                     GMHLNINVFNKDTLIDAQKHPEEYPTLTVRVSGYCVYFADLTKEQQDDVISRTFFESM"
     CDS             6066..7055
                     /locus_tag="ctg65_6"
                     /transl_table=11
                     /translation="MRMLEQIEQYIDSCIEAGDIYGASFSLITPKGINQYYHGKQGRDE
                     FAVGLDPAMIYDLASVTKVVGTTTRIFQLLSDHTIHLTDSVARSLPGFTHPEITIKNLL
                     LHNSGLPADIDNLDKMQREDLIKAVYDAPLVTAPGTTYVYSDLGFILLGWIIRAVDGSL
                     ARSIQDHVCYPLAMTNTGYNLNRPKIRFVPTEFDPRRGQIQGQVHDYKAFLLNGESGHA
                     GLFSTLTDLSVFIEMMLNFGEYQGKRVLDENVFDWLGEYDENGRTLGWERRAGRHQYFH
                     TGFTGPAVAFDLDRQIGLVVLTNRVYPTSDNQVWNQDRKRVFDLFFES"
     CDS             complement(7223..7738)
                     /locus_tag="ctg65_7"
                     /transl_table=11
                     /translation="MLAWLPFFLISLMFIVLLYACARSARTNIQRFKQLSLIAFLWVLT
                     AFCYTPTSYNFGTQLVIQYIQWGPVKLNVIPFQQLDIEFWLNVLLTVPLGGLLAWNFPD
                     LPWRRFIWLGFLTGLTLELGQFILDWLVNLKRWVDIDDVITNWAGVIIGAAGYKIARHV
                     PGLRWLQK"
ORIGIN
        1 taggtcgatc gtgattaagg taaatcgttg ccagctgcaa agtaaatgtc gtaccattct
       61 tgccgagaaa gcgtaacgtc tgctccagca gcattttgag ctagatgttc cggattcatc
      121 gtaccaagga tgacttggaa gtgggctggg tgccggagaa tccatgcggt cgcaatagct
      181 gatttagtga cctgtttctc atcggcgagt gcttgcatgg cattattaag ttctggaaac
      241 ttcggattgt ctaagaagat acccgcgaag ttaccgtatt gataaggcga ccaagcttga
      301 atcgtcatct tatggagccg tgagtattcg atcatctcgc cgtcgtggtt aagactacgt
      361 tcgtcctgca tgttcgtgtg caagccaaag tcgatcgcac cggtatgcat gataccaaat
      421 tggagttggt tgatgatgag ccgttggttt agtgccgctt gaagcatgtc aacttgcatg
      481 ggattgaagt tagagacgcc aaagtggcgc accttgccgc tagtttgcaa cttgtcaaaa
      541 gcggcagcaa cttcttcggg ttccatcagg gcgtccggtc gatggaggag gaaggtgtcg
      601 agataatcaa cacctaagcg ttgaagttcg ccatccacgg ctgccagtat gtgctgtttg
      661 gaaaagtcgt aacgcttgcc aggaacgatg ccgccttttg actgaatata gagctgatca
      721 cgcgatacgt gagcctgctt gagtgcttga ccgaagatgg tcgatgattg accaccaccg
      781 tagatatcag ctgaatcaac gtaattgatg cctaaatcaa cagcggtatc gataaccttg
      841 gtggcttgag cgacatccaa ggcgtccatg cgcatgatgc caagcgccac gctggaagct
      901 tgaatattgc tgtgacctaa attaatcgtt ttcaaaacta aagtcctcct caatgtgcaa
      961 cttgatttaa attcagtata cggttttcgt aagaggatgt aaacggttac gactggtgcc
     1021 gactgttggt aaatctgatt ttagagcaaa aaaaatagaa cccaaggctt ctctacttga
     1081 gttctactta tgaggaataa tcctgattag caatgaaaaa ttgttcagtg ttaatatgat
     1141 acaccttcat gctattcaat tttacgcgtg tccaacttct aacggactaa cccgtattta
     1201 ttgtaactat ctgctatagt cgatctaaag taaggttgtt acttgattgt gactcgttag
     1261 acgggtttta acgaaagtca aactcgcgtt taaaacttgg gaatgtcttc gtaacagaca
     1321 tgcacctccg tttaaacttt gtaatacctg agtctacaaa tggaactggt aatgtggaac
     1381 taacaactgg tcatcggaga cacctgtttc caactaaaac ttagttaaaa tctgttcgtc
     1441 attctaatgc caattaattc tttgcggtta tcgtttatct aagggtcaac gttcagtcat
     1501 ccaacgtaaa tctcacgaca ctcgctagac cgataacgct tcacggcgct taaaacggtt
     1561 tcgataacga cccaaactgt gatactagct gtctaacatt acagcgacag ccaaactctt
     1621 ttgagaagct ttgctaatcg aggattaatt cctttcctca cttagtataa tagcataaac
     1681 cgcaaacgct tacaacaaaa acaaccgcga attcgcaata aaaatcaata gtgactcaaa
     1741 acgctgctat atcggggatt atagtaaatt ttttcgagaa atagtcgcat aatttgctga
     1801 ttgaccgatc gtttgaaaag tcggctaatt agcgctaata attgattgaa tatgggctga
     1861 catacggtac aatagaagta gacaagaaag cgctttatat aacgatgacg tgtatcaagt
     1921 gtggtggcac ttataccgcg aaacgttggc gtgattaatc agtgaattca agctggtatg
     1981 gaaggacgag atacgatgac aacaacgttt aaagaccggg ccttcgaggc gctaggacat
     2041 gtcattgacc ctgaattagg aattgatcta gttaacttgg ggttgatcta tgatgcacaa
     2101 ttgacggcgg aggggaccgc tgaaattacc atgacgttga ccattgcggg ctgtcctttg
     2161 accgaatggt tggctaagga cattcacgat gaactgatca aattaccaga agttaacaat
     2221 atcgagattg agattgtgtg ggaacctgct tggaacgaaa gtatgatgtc ccgcgaagct
     2281 cgaatggaac taggcattca tagttagtgg ccggcctgtt cgcagctagt tcaagggctg
     2341 acggtcattg gatagggcat gattaaggaa agacgttgct ggtttaggct tgcggcgcct
     2401 tttttgcgtg ccactgtact aattccagcc agcaatacgt gcatgcgtcg agccagcaaa
     2461 aaaacaccag tgacacaacc atccgtagac gattgtatcg ctggtgtttt agtcatgcta
     2521 gaaatgccga tttaaagttt tggcaatggc atccatgtct tgtacccgtt gtaatcagcg
     2581 gtgtgcagta gtttttcggc atttgccacc cgatcttggg ttggagactc gattccttca
     2641 agccggtact tgatcttcat ttcatgatat tttttaacgc ccaaagtatg gtacggcaat
     2701 acttcgactt tttgaacgac gtcgtttgga atcttagcaa tgtaatcgcc gagtttcttc
     2761 aagtcttcat cgtaatcagt ccgttgggga accaggacgt gacgaatcca catatcatcg
     2821 tggtgttggg ccatgtactg aatcatatcc aagatatttt cgttcccata ctgggtcaac
     2881 tgcttgtgtt tggcagaatc gatgtgctta atgtcgacca acgaaatgtc cgtgaccttc
     2941 attagacgtt cgaacttgtc aaagaaaggt tgttctcggg taaaaggctg tccagaggta
     3001 tccagacaag tactgatatt catcgccttg gctttttcga ataagtctaa gatgaagtcg
     3061 atttgtacca gtgattcacc accactgact gtgatgccac ccgtcttgcc ccagaaagcc
     3121 cggtatttag ccgcgtcttc gagaatctcg tcggccgtca tttgatcgcc aacgttgagt
     3181 ttccaagtat cagggttgtg acagtattgg caacgcatgt ggcatccttg aaggaatgcc
     3241 acgtaacgga tacctggtcc gtcaacggag ccaaacgttt cgatggagtg aacgtagcct
     3301 atcaaaggct cctttgccgc cgcttgcgtt gttgaaactt gtttgttttc catgagcctt
     3361 tcgccttctt aactattaaa gattaaatgt tacattgatt cgaagaatgt ccgtgaaata
     3421 acgtcatctt gttgttcctt ggttaaatct gcgaagtaga cacagtagcc agaaacccga
     3481 accgttaatg ttgggtattc ttcagggtgt ttctgagcat caatcaaagt gtctttattg
     3541 aagacgttga tgttcaagtg catcccatcg ttcttcatgt aaccgtctaa catgtttact
     3601 aacgtgtctt tccgtgattc gaggtcatgg cctaacgtgt taggagtaac gccgaacgtg
     3661 ttggaaatcc cgtcagttgc gtaacggtat ggtaatttgg cagttgaaag aagtgaagct
     3721 aatgcaccac tcttttcagc accgtatgca gggttagcac ctggtgagaa tggttcgcct
     3781 ttttgacggc catttggtgt ggtaccagtg ttcttaccat aaacaacgtt ggaggtaatc
     3841 gtcaaaacag aagttgaaag tttggcattc cgatagaggt gatgcgtgtt catcttgctg
     3901 tataattctt tgactaacca tttagcaatg tcatcagcgc gatcgtcatt gttcccataa
     3961 cgtgggtagt cattgtcggc tttgaagtca acggcgatac cacgttcgtc acgaattact
     4021 ttaacgtgac catacttgat agctgagatt gaatccgcgg catgtgataa gccagaaatc
     4081 ccagttgcaa aggtccgatc aagatcagta ttcttcaaag ctaattgggc agcttcatag
     4141 tagtacttat catgcatata atgaattgca ttcagtgagt taacgtaagt gtcagctaac
     4201 caatccattt gcttgtctaa tttcttcatg aattcgtcgt aatcgatgta ttctgaagta
     4261 attggttcgt aagcagggcc aacttgatct ccagcaattt cgtcgcggcc accattgatg
     4321 gcataaagaa tcgctttggc taagtttgcc cgggcaccga agtactggat tccatcagca
     4381 attggttgtg cggaaacaca gcaagcaatg ccataataat cggtacccca ttgtacccgc
     4441 atcaagtcat cattttcata ctgaatggtt gaactgtcga ttgaaacttc agttgcgtaa
     4501 cgtttgaagt cttctggtaa gcggtctgac cataaaatcg tgatgtttgg ttctggtgcg
     4561 gcgcccatgt tgtccaaagt ctttaaaatc cggaaagcgg tcttagtcac atggtgttga
     4621 ccgtcgacgc ctaaaccaca cattgataag gttgcccaga ttgggtcacc agagaagaga
     4681 gaattgtatt cttcagtacg gatgaaccga accatccgta gtttcatgac gaattgatca
     4741 attaattctt gagcttggct ttcgtccaga acaccattgt ccaagtcacg ttggatgaag
     4801 atgtccatcg ttgtatcaat ccggccaacg gacattgcag cgccgttttg ggtcttgata
     4861 gcagctaagt aagcgaagta catccattga atagcttctt gagcattagt tgcaggcttg
     4921 ctaatatcgt agccataact ggcagccatc ttcttcatat ctgctaaagc acggtattga
     4981 tcttgaactt cttcacggag gcgaataaca tcatcagtca attcaccatc accaatgtgg
     5041 gcaaagtcat tagctttgtc ttccattaaa cgatcgatcc cataaacagc gacccgtgga
     5101 agatcaggaa tgagacggcc acgtgcgtat gcatctggta gtccggtgat gatcttgtag
     5161 tgacgtgctt tccgcatgtc aggagtataa acatcgaaga cgccttggtt atgagtcttg
     5221 cgatattcag tgaaaatctt gtcgttttct tcatcagggg tataaccgta tgatttcaaa
     5281 gcgtcatcag ccattcgaat accaccaaat ggcatgaagg cccgcttcaa aggcttgtca
     5341 gtctggagac caacaatctt ttcgagatct ttttgaatat aaccagggcc gtgtgaagta
     5401 atcgttgcaa cgactttagt atcagcatcc aacacaccac cagcggcacg ttcttgtttc
     5461 tttaattcta agactttgtc attcaaggtc ttagtagcgg ctgttggtcc ggccaggaag
     5521 ctttcgtcgc cattgtactg tgtgaagtta ttttgaataa aatcacgaat gttgatttct
     5581 tcctgccagt gcccgccttt gaaacctttc caagctgctg gttttgttgt cttttctgat
     5641 gtaatcattt tctcgcctcc ataatttgaa tccaatcaaa tgattgataa aattctatat
     5701 ttgaatcata acatttttcg ctggatttgt aaccgctttc aggtgattta tttcacgttt
     5761 gggttatttt tttgcaatct attattgaaa agttgactat tagcgttgat ataacagtac
     5821 ttacatagct agataggcat tggtaattca cttaattata tttagaaaac ggctaaaaac
     5881 aagacttgtt ttgatatata agtggaatat tgtccataat gtaacaagtg tttaactagt
     5941 taattaataa aaagtgcaac gaggtgtgga gggtgttaaa agcgtttgcc gttgatttta
     6001 tgaggaagtt cgttatcata aagaagataa gcagaaacta gtgaatgtag tgaaaggtgg
     6061 gcgtaatgcg gatgctcgaa caaattgaac aatatattga cagttgtatt gaagctggcg
     6121 acatttatgg ggcgtcgttt agcttgatca cccctaaagg tatcaatcaa tactatcatg
     6181 gtaagcaggg ccgcgatgag tttgccgtgg gcttagatcc tgcgatgatt tatgacctag
     6241 catcggtaac taaggttgta gggaccacga cgaggatttt tcagttgtta tcggaccaca
     6301 cgattcattt aacggactcg gttgcccggt ctttacctgg gttcactcat cctgaaataa
     6361 cgattaaaaa cttgttactt cataatagtg ggctaccggc agatattgat aacctggata
     6421 agatgcaacg tgaagatctg atcaaagcgg tttatgatgc gccactcgtc acggcaccag
     6481 ggacaacgta tgtctattca gacctgggat ttattttatt gggatggatt attcgtgcgg
     6541 ttgacggctc attggctcgg agtattcaag accacgtttg ctatccacta gcgatgacca
     6601 atacgggtta taacttaaat cgtccgaaga ttcggtttgt gccgacggag tttgatccgc
     6661 ggcggggtca gattcaagga caggtgcatg attataaagc ctttttgttg aatggtgaga
     6721 gtggtcacgc aggcttgttc tcaacgctga cggatttatc cgtatttatt gaaatgatgt
     6781 taaactttgg tgaatatcaa ggcaaacggg tgctggatga aaatgtcttt gactggttag
     6841 gcgagtatga cgaaaatggc cggacgttag gttgggaacg acgtgcggga cgacatcaat
     6901 atttccatac tggttttact ggcccggcag ttgcttttga tttggatcgt caaattgggc
     6961 tggttgtgtt aacaaatcga gtttatccaa ctagtgacaa tcaggtctgg aaccaagatc
     7021 gtaagcgagt attcgatctg ttttttgaat cctgatgatg gcagcttcat taatagttag
     7081 tagttaaatt aaataagcgt cgcagccact taaggctgcg acgcttattt aattacgatt
     7141 tcatgacagc tagaccgctg aagtgtttgg cacttgtggg tgctaagcaa acgctatttt
     7201 gctttttgct tctatatata cgttattttt gcaaccagcg aagtccggga acatgacgtg
     7261 caattttata accagcggca ccgataatga caccagccca atttgtgatg acatcatcga
     7321 tatcaaccca acgtttgagg ttaaccaacc agtcaaggat gaattgtccc agctcgagtg
     7381 ttagccccgt aagaaatccc aaccaaataa aacgacgcca gggcaagtcg ggaaaattcc
     7441 atgccagtaa accgcccaat ggaacagtga ggagaacgtt gagccaaaat tcaatgtcaa
     7501 gttgttggaa aggaatcacg ttcagcttaa cgggtcccca ttgaatatat tgaataacga
     7561 gttgggtacc aaaattgtaa ctggtaggtg tgtagcaaaa agcagttaag acccagagaa
     7621 aggcaattaa actgagttgc ttaaaacgct ggatatttgt gcgcgctgat cgcgcacaag
     7681 cgtatagtag aacaataaac attaatgaaa tcaaaaagaa tggtagccaa gctagcataa
     7741 tttagtcgct cctttaatta aatagtatag agaaagaaag ctggtttgac tattaataaa
     7801 aagaaattca gaaaaagtgt tgacactttt tacttcaatt ggtatattag taaacgttgc
     7861 tgctacggca gatacatctc accaccaaat aattattgaa attagttgtt gacaacttct
     7921 tcagtaattg gtaagatagt ttctgttgtc gcaatggcaa ccac
//
