GCA_900095345.1 - 12 region(s)

Identified secondary metabolite regions using strictness 'relaxed'

FMSN01000013.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 2.1	terpene	201,906	222,712

FMSN01000001.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 4.1	other,sactipeptide	323,234	386,000	subtilosin A	RiPP:Thiopeptide	100%
Region 4.2	lanthipeptide-class-i	619,174	645,399	subtilin	RiPP:Lanthipeptide	100%
Region 4.3	NRPS	812,773	859,912	bacillibactin	NRP	100%

FMSN01000005.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 5.1	NRPS	220,727	286,117	surfactin	NRP:Lipopeptide	86%

FMSN01000007.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 12.1	CDPS	1	13,814	micrococcin P1	RiPP:Thiopeptide	8%
Region 12.2	lanthipeptide-class-ii	122,912	149,228	thusin	RiPP	25%

FMSN01000009.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 14.1	transAT-PKS,PKS-like,T3PKS,NRPS	546,835	651,451	bacillaene	Polyketide + NRP	100%
Region 14.2	NRPS,betalactone,transAT-PKS	714,790	792,012	mycosubtilin	NRP + Polyketide	100%
Region 14.3	terpene	878,579	900,477
Region 14.4	T3PKS	948,915	990,012

FMSN01000010.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 15.1	lanthipeptide-class-v	251,910	293,794

Region	Type	From	To	Most similar known cluster	Similarity
Region 2.1	terpene	201,906	222,712
Region 4.1	other,sactipeptide	323,234	386,000	subtilosin A	RiPP:Thiopeptide	100%
Region 4.2	lanthipeptide-class-i	619,174	645,399	subtilin	RiPP:Lanthipeptide	100%
Region 4.3	NRPS	812,773	859,912	bacillibactin	NRP	100%
Region 5.1	NRPS	220,727	286,117	surfactin	NRP:Lipopeptide	86%
Region 12.1	CDPS	1	13,814	micrococcin P1	RiPP:Thiopeptide	8%
Region 12.2	lanthipeptide-class-ii	122,912	149,228	thusin	RiPP	25%
Region 14.1	transAT-PKS,PKS-like,T3PKS,NRPS	546,835	651,451	bacillaene	Polyketide + NRP	100%
Region 14.2	NRPS,betalactone,transAT-PKS	714,790	792,012	mycosubtilin	NRP + Polyketide	100%
Region 14.3	terpene	878,579	900,477
Region 14.4	T3PKS	948,915	990,012
Region 15.1	lanthipeptide-class-v	251,910	293,794

Compact view

FMSN01000013.1 - Region 1 - terpene

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Location: 201,906 - 222,712 nt. (total: 20,807 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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Gene details

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KnownClusterBlast

Similar known gene clusters

No matches found.

FMSN01000001.1 - Region 1 - other,sactipeptide

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Location: 323,234 - 386,000 nt. (total: 62,767 nt) Show pHMM detection rules used

other: (LmbU or Neocarzinostat or fom1 or bcpB or frbD or mitE or vlmB or prnB or CaiA or bacilysin or orf2_PTase)
sactipeptide: (subtilosin or thuricin or TIGR04404 or Subtilosin_A or skfc)

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KnownClusterBlast

Sactipeptides

Similar known gene clusters

Sactipeptide predictions

Putative Sactipeptide(s)

ctg4_369 leader / core peptide: MKKAVIVENK - GCATCSIGAACLVDGPIPDFEIAGATGLFGLWG

Sactipeptides

Core peptide predictions

ctg4_369: Putative sactipeptide; RODEO score: 33.0; Core sequence, approximate: GCATCSIGAACLVDGPIPDFEIAGATGLFGLWG

FMSN01000001.1 - Region 2 - lanthipeptide-class-i

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Location: 619,174 - 645,399 nt. (total: 26,226 nt) Show pHMM detection rules used

lanthipeptide-class-i: ((LANC_like and (Lant_dehydr_N or Lant_dehydr_C)) and not (YcaO or TIGR03882))

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Lanthipeptides

KnownClusterBlast

Lanthipeptide predictions

Lanthipeptide(s) for ctg4_616 - Putative Class I

ctg4_619 leader / core peptide: MSKFDDFDLDVVKVSKQDSKITPQ - WKDhaEDhaVCDhbPGCVDhbGLLQDhbCFLQDhbIDhbCNCKIDhaK

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Similar known gene clusters

Lanthipeptides

Core peptide predictions

ctg4_619 - Class I

Cleavage pHMM score: 27.80

RODEO score: 27

Monoisotopic mass: 3346.7 Da

Molecular weight: 3349.0 Da

Number of bridges: 5

Alternative weights: (assuming N unmodified Ser/Thr residues); 3367.0 Da N = 1; 3385.0 Da N = 2; 3403.0 Da N = 3

FMSN01000001.1 - Region 3 - NRPS

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Location: 812,773 - 859,912 nt. (total: 47,140 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 5, location 812772 - 859912:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (gly - thr)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg4_823

Search NORINE for peptide: strict or relaxed

AMP-binding (31..435): dhb

NRPSPredictor2: dhb

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: dhb, sal
Small clusters prediction:: dhb, sal
Single AA prediction:: dhb

Stachelhaus prediction details:

Stachelhaus sequence:: PLPAQGVVNK
Nearest Stachelhaus code:: dhb
Stachelhaus code match:: 100% (strong)

ctg4_825

Search NORINE for peptide: strict or relaxed

AMP-binding (463..861): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGLIWK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 100% (strong)

AMP-binding (1519..1935): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

FMSN01000005.1 - Region 1 - NRPS

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Location: 220,727 - 286,117 nt. (total: 65,391 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))

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Gene details

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 220726 - 286117:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (glu - leu - D-leu) + (val - asp - D-leu) + (leu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg5_257

Search NORINE for peptide: strict or relaxed

AMP-binding (469..867): leu

NRPSPredictor2: leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: leu

Stachelhaus prediction details:

Stachelhaus sequence:: DAFFLGCVFK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 100% (strong)

ctg5_258

Search NORINE for peptide: strict or relaxed

AMP-binding (470..864): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: val

Stachelhaus prediction details:

Stachelhaus sequence:: DAFWIGGTFK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 100% (strong)

AMP-binding (1501..1904): asp

NRPSPredictor2: asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asp

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKVGHIGK
Nearest Stachelhaus code:: asp
Stachelhaus code match:: 100% (strong)

AMP-binding (2546..2934): leu

NRPSPredictor2: leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: leu

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFLGNVVK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 100% (strong)

ctg5_259

Search NORINE for peptide: strict or relaxed

AMP-binding (466..867): glu

NRPSPredictor2: glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: glu, gln
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAKDLGVVDK
Nearest Stachelhaus code:: glu
Stachelhaus code match:: 100% (strong)

AMP-binding (1510..1908): leu

NRPSPredictor2: leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: leu

Stachelhaus prediction details:

Stachelhaus sequence:: DAFMMGMVFK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 100% (strong)

AMP-binding (2550..2938): leu

NRPSPredictor2: leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: leu

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFLGNVVK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 100% (strong)

FMSN01000007.1 - Region 1 - CDPS

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Location: 1 - 13,814 nt. (total: 13,814 nt) Show pHMM detection rules used

Region on contig edge.

CDPS: (CDPS or cycdipepsynth)

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KnownClusterBlast

Similar known gene clusters

FMSN01000007.1 - Region 2 - lanthipeptide-class-ii

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Location: 122,912 - 149,228 nt. (total: 26,317 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

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Lanthipeptides

KnownClusterBlast

Lanthipeptide predictions

Lanthipeptide(s) for ctg12_129 - Putative Class II

ctg12_128 leader / core peptide: MEKTKILKTPELKVTKDLNLIDPCGDVLEEIKEHEVSGA - FNDhbWNLDhbDhbDhbDhaVGDhbILVDhaEVFGNKGKVCDhbGDhbKECQGDhbC
ctg12_130 leader / core peptide: MKKDMVKNASGFIEEDELISLANGENATGG - GDhbPVDhbAIIDhbALDhbGDhbDhbFDhbVDhbLDhaAADhaCPDhbDhaACDhbNLCNK
allorf_124854_124994 leader / core peptide: MKTGFQKTFCDRETTAFLAA - LPALDhaPKAAALADhaDELPCAEKDCDLP
allorf_130261_130350 leader / core peptide: LEGILGM - KDhaFLEKADhaKALKLCVMVADhbIYV
allorf_132238_132315 leader / core peptide: LSHLMGA - VPIDhbICDhaLLAKGRAYVLQ

Lanthipeptide(s) for ctg12_131 - Putative Class II

ctg12_128 leader / core peptide: MEKTKILKTPELKVTKDLNLIDPCGDVLEEIKEHEVSGA - FNDhbWNLDhbDhbDhbDhaVGDhbILVDhaEVFGNKGKVCDhbGDhbKECQGDhbC
ctg12_130 leader / core peptide: MKKDMVKNASGFIEEDELISLANGENATGG - GDhbPVDhbAIIDhbALDhbGDhbDhbFDhbVDhbLDhaAADhaCPDhbDhaACDhbNLCNK
allorf_130261_130350 leader / core peptide: LEGILGM - KDhaFLEKADhaKALKLCVMVADhbIYV
allorf_132238_132315 leader / core peptide: LSHLMGA - VPIDhbICDhaLLAKGRAYVLQ

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Similar known gene clusters

Lanthipeptides

Core peptide predictions

ctg12_128 - Class II

Cleavage pHMM score: -3.20

RODEO score: 0

Monoisotopic mass: 3742.9 Da

Molecular weight: 3745.2 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 3763.2 Da N = 1; 3781.3 Da N = 2; 3799.3 Da N = 3; 3817.3 Da N = 4; 3835.3 Da N = 5; 3853.3 Da N = 6; 3871.4 Da N = 7

ctg12_130 - Class II

Cleavage pHMM score: -5.20

RODEO score: 0

Monoisotopic mass: 3294.8 Da

Molecular weight: 3296.8 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 3314.8 Da N = 1; 3332.8 Da N = 2; 3350.8 Da N = 3; 3368.9 Da N = 4; 3386.9 Da N = 5; 3404.9 Da N = 6; 3422.9 Da N = 7; 3440.9 Da N = 8; 3459.0 Da N = 9; 3477.0 Da N = 10

allorf_130261_130350 - Class II

Cleavage pHMM score: -4.00

RODEO score: 0

Monoisotopic mass: 2387.4 Da

Molecular weight: 2389.0 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2407.0 Da N = 1; 2425.0 Da N = 2

allorf_132238_132315 - Class II

Cleavage pHMM score: -7.20

RODEO score: 0

Monoisotopic mass: 1908.1 Da

Molecular weight: 1909.3 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1927.4 Da N = 1

ctg12_128 - Class II

Cleavage pHMM score: -3.20

RODEO score: 0

Monoisotopic mass: 3742.9 Da

Molecular weight: 3745.2 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 3763.2 Da N = 1; 3781.3 Da N = 2; 3799.3 Da N = 3; 3817.3 Da N = 4; 3835.3 Da N = 5; 3853.3 Da N = 6; 3871.4 Da N = 7

ctg12_130 - Class II

Cleavage pHMM score: -5.20

RODEO score: 0

Monoisotopic mass: 3294.8 Da

Molecular weight: 3296.8 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 3314.8 Da N = 1; 3332.8 Da N = 2; 3350.8 Da N = 3; 3368.9 Da N = 4; 3386.9 Da N = 5; 3404.9 Da N = 6; 3422.9 Da N = 7; 3440.9 Da N = 8; 3459.0 Da N = 9; 3477.0 Da N = 10

allorf_124854_124994 - Class II

Cleavage pHMM score: -6.00

RODEO score: 0

Monoisotopic mass: 2601.3 Da

Molecular weight: 2603.0 Da

Number of bridges: 2

allorf_130261_130350 - Class II

Cleavage pHMM score: -4.00

RODEO score: 0

Monoisotopic mass: 2387.4 Da

Molecular weight: 2389.0 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2407.0 Da N = 1; 2425.0 Da N = 2

allorf_132238_132315 - Class II

Cleavage pHMM score: -7.20

RODEO score: 0

Monoisotopic mass: 1908.1 Da

Molecular weight: 1909.3 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1927.4 Da N = 1

FMSN01000009.1 - Region 1 - NRPS,PKS-like,T3PKS,transAT-PKS

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Location: 546,835 - 651,451 nt. (total: 104,617 nt) Show pHMM detection rules used

transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))
PKS-like: (t2pks2 or ksIII)
T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)
NRPS: cds(Condensation and (AMP-binding or A-OX))

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 546834 - 651451:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (gly - ccmal - ohmal - mal) + (mal - ccmal - mal - ohmal - mal) + (mal - ccmal - ohmal - mal) + (X - ccmal - ccmal - ccmal) + (ccmal - mal - mal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 546834 - 633336:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (gly - ccmal - ohmal - mal) + (mal - ccmal - mal - ohmal - mal) + (mal - ccmal - ohmal - mal) + (X - ccmal - ccmal - ccmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg14_591

PKS_AT (4..283): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 102.4
Methoxymalonyl-CoA: 50.8
inactive: 41.9
Methylmalonyl-CoA: 37.6
Propionyl-CoA: 23.8
2-Methylbutyryl-CoA: 12.8
Ethylmalonyl-CoA: 12.7
Isobutyryl-CoA: 10.7
CHC-CoA: 10.4
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

ctg14_592

PKS_AT (7..295): pk

ATSignature: (unknown)

No matches above 50%

minowa: inactive

Prediction, score:

inactive: 32.1
Malonyl-CoA: 25.1
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ctg14_593

PKS_AT (4..284): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 66.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 85.0
inactive: 40.8
Methoxymalonyl-CoA: 27.0
Methylmalonyl-CoA: 15.3
Propionyl-CoA: 11.0
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ctg14_599

Search NORINE for peptide: strict or relaxed

CAL_domain (60..476): pk

minowa: fatty_acid

Prediction, score:

fatty_acid: 19.8
NH2: 10.4
shikimic_acid: 0.0
Acetyl-CoA: 0.0
AHBA: 0.0

AMP-binding (1159..1554): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGMIWK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 100% (strong)

ctg14_602

Search NORINE for peptide: strict or relaxed

AMP-binding (498..906): X

NRPSPredictor2: hydrophobic-aliphatic

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVsnmAiIYK
Nearest Stachelhaus code:: ala
Stachelhaus code match:: 60% (weak)

FMSN01000009.1 - Region 2 - NRPS,betalactone,transAT-PKS

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Location: 714,790 - 792,012 nt. (total: 77,223 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))
betalactone: (HMGL-like and AMP-binding)
transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))

Legend:

core biosynthetic genes

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Gene details

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 3, location 714789 - 792012:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal - asn) + (D-tyr - D-asn - gln - pro) + (D-ser - asn)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg14_707

Search NORINE for peptide: strict or relaxed

AMP-binding (13..445): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: N/A
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: gclhIAApvK
Nearest Stachelhaus code:: phe
Stachelhaus code match:: 40% (weak)

ctg14_712

Search NORINE for peptide: strict or relaxed

AMP-binding (268..673): ser

NRPSPredictor2: ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: ser
Single AA prediction:: ser

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHFSLIDK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 100% (strong)

AMP-binding (1788..2183): asn

NRPSPredictor2: asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asn

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKIGEVGK
Nearest Stachelhaus code:: asn
Stachelhaus code match:: 100% (strong)

ctg14_713

Search NORINE for peptide: strict or relaxed

AMP-binding (268..662): tyr

NRPSPredictor2: tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: phe, trp, phg, tyr, bht
Small clusters prediction:: tyr, bht
Single AA prediction:: tyr

Stachelhaus prediction details:

Stachelhaus sequence:: DALSVGEVVK
Nearest Stachelhaus code:: tyr
Stachelhaus code match:: 100% (strong)

AMP-binding (1778..2173): asn

NRPSPredictor2: asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asn

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKIGEVGK
Nearest Stachelhaus code:: asn
Stachelhaus code match:: 100% (strong)

AMP-binding (3289..3694): gln

NRPSPredictor2: gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: glu, gln
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAQDLGVVDK
Nearest Stachelhaus code:: gln
Stachelhaus code match:: 100% (strong)

AMP-binding (4338..4734): pro

NRPSPredictor2: pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: pro, pip
Small clusters prediction:: pro
Single AA prediction:: pro

Stachelhaus prediction details:

Stachelhaus sequence:: DVQFIAHVVK
Nearest Stachelhaus code:: pro
Stachelhaus code match:: 100% (strong)

ctg14_714

Search NORINE for peptide: strict or relaxed

CAL_domain (36..479): pk

minowa: fatty_acid

Prediction, score:

fatty_acid: 617.1
NH2: 15.6
shikimic_acid: 0.0
Acetyl-CoA: 0.0
AHBA: 0.0

AMP-binding (2945..3340): asn

NRPSPredictor2: asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asn

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKIGEVGK
Nearest Stachelhaus code:: asn
Stachelhaus code match:: 100% (strong)

ctg14_715

PKS_AT (6..293): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; inactive: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 85.1
inactive: 46.5
Methoxymalonyl-CoA: 28.9
Methylmalonyl-CoA: 13.8
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

FMSN01000009.1 - Region 3 - terpene

Download region GenBank file

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Location: 878,579 - 900,477 nt. (total: 21,899 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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KnownClusterBlast

Similar known gene clusters

No matches found.

FMSN01000009.1 - Region 4 - T3PKS

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Location: 948,915 - 990,012 nt. (total: 41,098 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

KnownClusterBlast

Similar known gene clusters

No matches found.

FMSN01000010.1 - Region 1 - lanthipeptide-class-v

Download region GenBank file

Download region SVG

Location: 251,910 - 293,794 nt. (total: 41,885 nt) Show pHMM detection rules used

lanthipeptide-class-v: (APH and HopA1)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

Lanthipeptides

KnownClusterBlast

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Similar known gene clusters

No matches found.

Lanthipeptides

Core peptide predictions

No core peptides found.