LOCUS       c00081_CABFLA0..        5967 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  CABFLA010000159.1 Lactobacillus rhamnosus isolate AMBR6 genome
            assembly, contig: gnl|Prokka|CKHPCMOK_74, whole genome shotgun
            sequence.
ACCESSION   CABFLA010000159
VERSION     CABFLA010000159
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2022-06-21 12:11:14
            Original ID  :: CABFLA010000159.1
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5967
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5967
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2364:5615]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2365..5615
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5967
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5967
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(313..1245)
                     /locus_tag="ctg81_1"
                     /transl_table=11
                     /translation="MTTLIFGHQNPDTDAITSAMSWAEFQKQAGNTDVEAVALGEPNDE
                     TKFVLDHFKVAAPRVIKTAANETGHVMLVDHNEFQQSVADIEDVQIDSVVDHHRIANFH
                     TASPLYYRAEPVGSTNTVIFELFQEHGYEISPTLAGLMASGLISDTLLLKSPTATDKDK
                     KILPEMAKIAGIDLQSYGLAMLKAGTNLAAKSDMDIIEGDAKSFTMGGKSIRIGQVNTV
                     DLDDIYARQDELVAAMTKEAQAKGYDDFLLIATDILNSNSTGFAVGADRDKVAAAFNEK
                     FDANDRLALPGVVSRKKQVVPPMQGAFEG"
     CDS             complement(1275..2243)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) LysR_substrate"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HTH_1"
                     /gene_functions="regulatory (smcogs) SMCOG1012:Bacterial
                     regulatory helix-turn-helix protein, lysR family (Score:
                     55.5; E-value: 1.4e-17)"
                     /gene_kind="regulatory"
                     /locus_tag="ctg81_2"
                     /sec_met_domain="LysR_substrate (E-value: 7.1e-28,
                     bitscore: 85.5, seeds: 361, tool: rule-based-clusters)"
                     /sec_met_domain="HTH_1 (E-value: 8.8e-19, bitscore: 55.5,
                     seeds: 1330, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTKQERIFSSKTLTYFLQLAETMNYTQSAQLLGITQPALTQQIK
                     KLERTVGAPLFYSVGKKLHLSDAGRTMLKATHEIYEILNQTTDEIQQSTSATVGKINIG
                     LLSSIEDSAFNAFIAHYYDLNPEVEITLRMMTRHEIWEALENNQIDLAIMNLPDETIKN
                     WKPYASKKILDEQLLFLHHDNSLANRKRLHFKDTLAEPWVTYPDDYFLGQLLHEAYKNQ
                     MVDFPHVVAHFTTPYQLTQFVNHTGVNTALPTSFVAAHADQIKAHAVAFEPAIRFETSF
                     VYRKEKADIPRIANFFKTFDAHLSEKDYLSRVSENSGKIER"
     CDS             complement(2365..3216)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 66.6;
                     E-value: 9e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg81_3"
                     /sec_met_domain="Radical_SAM (E-value: 1.9e-27, bitscore:
                     84.8, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLIDQSQSPLNILEQVGQDHEGPIKGYVHSVESFGSVDGPGIRF
                     VVFMQGCRMRCQYCHNPDTWNIGVGEEMTADQILADAQRYKAFWGEQGGITCSGGESLV
                     QIDFILELFTKAKELGISTCLDTSGGPFTRDQPWFGQFEKLMAVTDISLVDIKHIDSAE
                     HKKLTGFPNENILDMVQYMSDHGDDMWIRHVLVPERTDFDPYLKRLGDYIATLDKRVVQ
                     KVEILPYHTLGVKKYHELGITYPLEGIEPPSADRVKNAENLLHVEDYTGWQSWRPQPAN
                     KQ"
     CDS             complement(3354..5615)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg81_4"
                     /sec_met_domain="Gly_radical (E-value: 1.6e-42, bitscore:
                     132.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1e-245, bitscore:
                     804.8, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKQLDETKVPNYWEGFNGGDWQEEINVRDFIEHNLNQYDGDESFL
                     AGPTEATTVLNNQVLNLKKQERANGGVLDADNNIPSTITSHGPGYLNKDLEKIVGVQTD
                     KPFKRAFMPFGGIRMAEDALKSYGFETDANEHKIFTEYRKTHNQGVFDAYTPEMRKARH
                     YKIVTGLPDAYGRGRIVSDFPRIAIYGIDRLMAEKFNDYNLTGDGEMTDDVIKLREEIN
                     EQYRALNDMKKMAKEYGYDISRPAANAQEAVQWIYFGYLAAVKTQNGAAMSVGRIDTVI
                     DAYIQRDMRLGKLNEEQAQELIDHLVMKLRMVRFIRTEEYNSLFSGDPIWATLSLAGMG
                     NDGRHHVTKTAFRFLKTLDNMGAAPEPNITLLWSERLPEGFKRYATEVSIQSSTIQYEN
                     DDLMRNEWGTDYYGIACCVSAQPIADGVQYFGARANLAKTVLYAINGGKDEIQEAQVGP
                     EYEPITSDYIDYDEFMKKFDKMMDWLADVYVNALNVIHYMHDKYYYEAAQLALKDTRLN
                     RTFATGISGLSHAVDSISAIKYGHVKAIRDENGVAIDFVADNDDYPRYGNNDDRADNIA
                     KWLVKTFYNKMNTHHLYRGAKLSTSVLTITSNVVYGKNTGTTPNGRQKGEPFSPGANPA
                     YGAEKNGALASLMSTAKIPYHYATDGISNTFGVTPNTLGHDDETRKDTLVHMVDGYMEN
                     SGMHLNINVFNKETLIDAQKHPEEYPTLTVRVSGYCVYFADLTKEQQDDVIARTFFDEM
                     "
ORIGIN
        1 agtcaaatat aattcatttc tttttttttt aatgatacgg agaccaccga gatctacacc
       61 taagccttcg tcggcagcgt cagatgtgta taagagacag cataatatat attatacgaa
      121 gtagttataa ggctgcgttt tttgcacctc gttttaaatt tgaactattc ttactgaaaa
      181 atttcgcaat aatcacattt ctgcaccttc agatctaaaa attcgacttt tttgttcttc
      241 caagtttttg agcacaaaaa aatcgcaacc acaaacggct gcgatttata aaaccaatta
      301 ttaagttcaa aattaacctt caaacgcacc ttgcattggc ggtacgacct gcttcttacg
      361 tgaaaccacg ccaggcagtg ccaaccggtc attggcatca aacttttcgt tgaatgctgc
      421 ggccactttg tcacgatctg cgccaacagc aaagcccgtt gaattgctgt tcagaatatc
      481 ggttgcaatt aacaggaagt catcatagcc cttcgcctgt gcttctttgg tcatagcggc
      541 caccaattca tcctgacgcg cataaatatc atcaagatca actgtattca cttgtccaat
      601 acggatagac ttaccgccca tcgtaaagct cttcgcatcg ccttcaatga tatccatatc
      661 cgacttggca gctagattgg tgcccgcctt caacatagcc aaaccgtatg attgcaggtc
      721 aatgccagcg attttagcca tttccggcaa gatctttttg tccttgtcgg ttgcagtcgg
      781 tgacttcaga agcagggtat ccgaaatcaa gccagaagcc attaagcctg ccagtgttgg
      841 tgaaatttca tagccgtgtt cctggaacaa ctcgaaaatc accgtgttgg tactgcccac
      901 tggttctgcc cggtaatata gtggtgacgc cgtatggaag ttggcaatgc ggtggtgatc
      961 aaccactgaa tcgatttgca cgtcttcgat atcagcaacg ctttgctgga attcgttatg
     1021 atcaaccaac attacgtgcc cggtttcatt tgcggctgtc ttaatgaccc gcggcgcagc
     1081 aaccttaaaa tgatccaata caaacttggt ttcatcgttt ggttcaccga gtgctactgc
     1141 ttcaacatcg gtattacctg cttgcttctg aaattcagcc caactcatgg cgcttgtaat
     1201 cgcgtctgta tcagggtttt gatgtccaaa aatcaaagtc gtcatgttat ctgatcgctc
     1261 ctcagctgaa tagtttacct ttcaatttta ccactgtttt cgctgacccg cgatagataa
     1321 tctttctcag ataaatgtgc gtcaaacgtc ttaaagaagt tggcaatccg cggaatatca
     1381 gctttttcct tgcgatacac aaacgaggtc tcaaagcgaa tcgctggttc aaaggcaacg
     1441 gcatgcgcct tgatttgatc agcatgagcg gcaacaaaac tggttggtaa cgcggtgtta
     1501 acaccggtat ggttgacaaa ctgagttaac tgatatggcg tggtaaaatg cgccacaaca
     1561 tgcggaaaat caaccatctg attcttatat gcctcatgca acaactgacc caggaaatag
     1621 tcgtctggat aagttaccca cggctcagcc agcgtatctt tgaaatgcag gcgtttgcga
     1681 ttagccaggg aattatcatg gtgcaggaac aataactgtt catcaagaat cttcttgctg
     1741 gcatacggtt tccagttctt aatcgtctca tcaggcagat tcatgatggc caaatcaatc
     1801 tggttgttct ccaatgcttc ccagatctca tgacgtgtca tcattcgcaa cgtgatttct
     1861 acttcaggat taagatcata gtagtgcgcg ataaaagcat taaacgcact gtcttcaatt
     1921 gaacttaaca agccaatatt aatcttaccg actgtggcac tggtactttg ctgaatctcg
     1981 tcagtcgtct ggttcaaaat ttcataaatc tcgtgtgtcg ccttcaacat cgtgcgaccg
     2041 gcatcggata aatgcaactt cttaccaacg gaataaaaca atggtgcccc cactgttcgt
     2101 tccaatttct tgatttgctg cgtcagtgcc ggctgggtaa tccccaacaa ctgggctgac
     2161 tgggtgtagt tcatggtttc tgctaattgc aaaaaatacg tgagagtttt ggaggaaaaa
     2221 atgcgctctt gtttcgtctt catagggcgg cttccctttc gtttataatt tgataacgtt
     2281 actcaaccat catcataagt ggcatttaaa aaaaacgcaa cggatttgct gcgtttttaa
     2341 tcgttacgtt aagtttcaac taaatcactg cttgttagct ggctgtggtc gccaactttg
     2401 ccatccagta tagtcctcaa cgtgtagcaa gttctctgca ttcttgaccc gatcagctga
     2461 aggcggctcg ataccttcca gcggatacgt aatacctagt tcatggtact tcttaacccc
     2521 taacgtatgg tacggaagaa tttccacttt ctggaccacg cgtttatcca aagtggcaat
     2581 ataatctccc aagcgtttga gataaggatc aaagtcggtt cgttctggca caagtacgtg
     2641 gcgaatccac atatcatcgc catgatccga catatactga accatatcca aaatgttttc
     2701 gtttgggaaa ccggtcagct ttttgtgttc cgcggaatca atgtgcttga tatcaaccaa
     2761 cgaaatatcc gtgacggcca tcagtttctc aaattgacca aaccacggct gatcgcgggt
     2821 gaatggacca cccgaagtat ccaaacaagt tgagataccc agttccttag ctttggtaaa
     2881 aagttccagg ataaaatcaa tttggacgag actttcgcca ccgctacaag taatcccccc
     2941 ttgttcaccc cagaaggctt tatatcgctg tgcatctgcc aggatctgat cagcggtcat
     3001 ttcctcgccc acaccgatat tccaagtgtc agggttgtga cagtactggc accgcatcct
     3061 gcatccttgc ataaagacga caaaccgaat cccgggacca tcaaccgaac caaagctttc
     3121 gactgaatga acgtatccct ttattgggcc ttcatgatcc tgtcccactt gttccaaaat
     3181 atttaacggg ctttgtgatt gatcgatgag tttcatctat gccgcctcca gtgcaaataa
     3241 atacctaatt ttcaacgatt ttcatttcag tttgtcaaaa aacggcaaca gcatcgttgc
     3301 cgttttttga caaaacagga atgatctcct gttttgcgaa tagattgact tgattacatt
     3361 tcgtcgaaga aggtccgagc gataacatcg tcttgctgtt ctttggtcag gtctgcaaag
     3421 tagacgcagt aaccggaaac tcgaacagtc aaagttggat attcttctgg gtgcttctgg
     3481 gcatcaatca acgtttcttt attgaagacg ttgatgttaa ggtgcatgcc gctattttcc
     3541 atgtagccgt caaccatgtg aaccaaggtg tccttacgag tttcatcgtc atggcctaag
     3601 gtgttcggtg ttaccccaaa ggtattgctg atcccgtctg ttgcatagtg atatgggatc
     3661 ttggcggttg acatcaagga agctaaagca ccgttctttt cagcgccata cgctgggttg
     3721 gctccaggtg agaatggttc gcccttctga cggccgtttg gtgttgtccc ggtattctta
     3781 ccataaacca catttgatgt aatggtcaga acactggtac tgagcttagc accgcgatac
     3841 agatgatgtg tattcatctt gttgtagaag gtcttaacca accacttagc aatgttgtct
     3901 gcccggtcat cattgttacc ataacgcgga tagtcatcat tgtcagcaac aaagtcgatt
     3961 gcaacgccat tttcatcccg aattgccttc acgtggccat acttgatggc actgatggaa
     4021 tcaaccgcat gtgacaaacc ggaaatcccg gtagcaaacg tccgattcag acgtgtatcc
     4081 ttcaatgcca actgcgcagc ttcatagtag tacttgtcgt gcatgtagtg aataacattc
     4141 agggcattga catagacatc tgctaaccag tccatcatct tgtcgaactt cttcatgaac
     4201 tcgtcatagt caatgtagtc gctggtgatc ggctcgtatt caggtcccac ctgtgcttcc
     4261 tgaatttcgt ccttaccacc gttgatcgca tacagcacgg tcttggctag gtttgctcgc
     4321 gccccgaagt actgaacacc atctgcaatt ggctgtgcgg aaacacagca agcaatgccg
     4381 taatagtcgg ttccccattc gttgcgcatc agatcatcgt tttcgtactg aatggttgaa
     4441 ctttggatgg acacttcagt agcataacgt ttgaaccctt caggaagacg ttcgctccac
     4501 aacaaggtga tgtttggttc cggtgccgcg cccatgttgt ccaaggtctt caggaagcgg
     4561 aacgcggtct tggtaacatg atgacggcca tcattaccca tccctgccaa ggacagtgtt
     4621 gcccagatcg gatcaccgga gaacagtgag ttgtattctt cagtccgaat gaaacgtacc
     4681 atccgtaact tcataaccaa atgatcgatg agttcttgtg cctgctcttc attcagcttg
     4741 cccaaacgca tatcccgttg aatataggca tcaatcactg tatcgatacg gccgacagac
     4801 attgccgcac cattttgggt cttaacggct gccaggtaac cgaagtagat ccactgaaca
     4861 gcttcttgag cattagcagc tgggcgggaa atgtcatagc cgtattcttt ggccatcttc
     4921 ttcatgtcat tcaaggcacg atactgttca ttaatttctt cgcgcagttt gataacatca
     4981 tctgtcatct cgccatcgcc ggtcaggttg taatcgttga acttctcagc catcagccgg
     5041 tcaatcccat aaatagcaat tcgagggaag tccgaaacga tccgaccacg accataagca
     5101 tccggtaagc cggtcacaat cttatagtgg cgggctttgc gcatttccgg cgtataagca
     5161 tcaaaaacac cttggttatg cgtcttacga tattcggtaa agatcttgtg ctcattagcg
     5221 tcggtttcga agccatagga tttcaaagca tcctcagcca tccggatacc gccaaatggc
     5281 atgaatgctc gcttaaacgg cttatcggtt tggacaccga caatcttttc gaggtcctta
     5341 ttcagataac cggggccatg agaagtaatg gtcgatggaa tattgttatc tgcatccagt
     5401 acaccgccat tggcacgttc ttgctttttc agattcaaaa cctgattatt taaaacagtc
     5461 gttgcttcag tggggccagc aaggaaactc tcgtccccat catattggtt caggttatgc
     5521 tcgataaaat cgcgcacgtt aatttcttct tgccagtcgc cgccgttaaa gccttcccaa
     5581 taattaggaa ctttggtttc gtcaagctgt ttcataggta ttgcctccta aagtgaatta
     5641 cagatgtgac agttcttaca aactcagtat aacacgaagg caaatctatg caagcgtttt
     5701 atcgctgata taacaggcgt tttgctgttt ttttcggtgt actttcctgt ttcataaaaa
     5761 aagcttaatc attagcttga taagttgtgc acaacctgtc tcttatacac atctccgagc
     5821 ccacgagact aaggcgaatc tcgtatgccg tcttctgctt gaaaaaaaaa aaaaaagaaa
     5881 aaaaaaaaaa aaaaaaaaca aaagagaatt gcgagagacg aagtataggg aggtggtagg
     5941 aacacggcag aagaaagaaa gagaaca
//
