LOCUS       c00478_CADBNU0..        7527 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  CADBNU010000478.1 Bacillus coagulans isolate RUG14133 genome
            assembly, contig: RUG14133_asm_478, whole genome shotgun sequence.
ACCESSION   CADBNU010000478
VERSION     CADBNU010000478
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2022-06-21 02:30:25
            Original ID  :: CADBNU010000478.1
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7527
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..171)
                     /locus_tag="ctg478_1"
                     /transl_table=11
                     /translation="MNSFLKGTIILAGAAFISECIEFLINLTLAKELGEYGIGQYMTVI
                     PVIGLLMIIASF"
     protocluster    1..7527
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[539:3632]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      540..3632
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7527
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7527
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             540..2768
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg478_2"
                     /sec_met_domain="Gly_radical (E-value: 6.1e-46, bitscore:
                     144.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 6.2e-300, bitscore:
                     984.8, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKQWEGFKPGKWQEKVDVRDFILKNVTPYEGDESFLEGPTEATTN
                     LWEQVMELNKKEREAGGILDADTKVVSTITSHGPGYLNKELEQIVGFQTDKPFKRSLMP
                     FGGIRMAKQALEAYGFELDPEVEHIFTEWRKTHNQGVFDAYTPEMRRARSAGVITGLPD
                     AYGRGRIIGDYRRVALYGVDFLIRQKEEDKNYIGSRTMTPDIIRDREEHSEQIRALHEL
                     KQMAASYGFDISEPASNAKEAIQWLYFGYLAAIKEQNGAAMSLGRVSTFLDIYIERDLK
                     NGTLTEREAQELVDHFVMKLRLVKFARTPEYNELYSGDPTWVTESIGGMALDGRTLVTK
                     NSFRFLNTLDNLGPAPEPNLTILWSTKLPEGFKKYAAKMSIKSSAIQYENDDLMRDIYG
                     DDYGIACCVSAMRIGKQMQFFGARVNVAKALLYAINGGVDEMKKVQVGPKFAPITSEYL
                     EYEEVVDKFDAMLDWLCELYVNTLNVIHYMHDKYAYERIEMALHDREVLRTMACGIAGL
                     SVAADSLSAIKHAKVKTIRDENGIVVDYEVEGDFPKYGNNDDRVDEIAVNIVKKVMKNI
                     RKHQTYRNAVPTQSVLTITSNVVYGKKTGNTPDGREAGKPFAPGANPMHGRDTKGALAS
                     LTSVAKLPYEDSLDGISNTFTITPKAIGRDDEAQVNNLVGMLDGYMTKEGHHLNVNVLN
                     RDTLLDAMDHPELYPQLTIRVSGYAVNFIKLTREQQIDVINRTFHQSM"
     CDS             2901..3632
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 80.2;
                     E-value: 5.7e-25)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg478_3"
                     /sec_met_domain="Radical_SAM (E-value: 1.6e-32, bitscore:
                     102.3, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVTGRIHSIETFGAVDGPGLRYIVFMQGCSLRCLYCHNADTWKMK
                     SGKEITVQEIIDEVLDYMPFFEASGGGITVSGGEALLQIDFLIELFKECKRHGIHTTLD
                     TSGGPFSRRPNYLNKLDELLKYTDLILLDLKHIDSEKHKLLTGMHNEHILDFATYLSDK
                     KIPVWIRHVLVPTISDFDEDLYRLAEFIQTLDNVEKVEVLPYHQLGVYKWENLGLEYKL
                     KNVDPPTQERIKNAEKILGAS"
     CDS             4145..4765
                     /locus_tag="ctg478_4"
                     /transl_table=11
                     /translation="MPISFPVVIQTRKVSDGPKKYVYYPQIIELKNKQVEQHINRTIVH
                     HTQQLMNQQFGNMPSIIVEMVGSFEIKNNQRDILSLTLSNYAYPDRAAHGMTYLDSLTF
                     NVETGKQYALKDLFKPGSDYIERISNIIKGQIKQRDLPVITEFTTIKPDQDYYIADKAL
                     VIYFQLYDLVPYAWGFPMFPISVYDIQDIIDENGPLGKMLAGN"
     CDS             5277..5420
                     /locus_tag="ctg478_5"
                     /transl_table=11
                     /translation="MEYITLSFPPFPIFIKGAESVFPKGQKHFRRTFSVFELLYVKRDA
                     YI"
     CDS             5417..5587
                     /locus_tag="ctg478_6"
                     /transl_table=11
                     /translation="MTEENKEFAVKEGKYLILIPGREHYGHYPCTEETQLVWLHFTIEG
                     GYDIVPKQSLS"
     CDS             5690..6139
                     /locus_tag="ctg478_7"
                     /transl_table=11
                     /translation="LLQQLVELNEERDPDRSLKQLLYFVEFIWQLQKQELHIPSASEKV
                     CAEAVAYIERHYAEPITIQQLAQSLRFHPDYITRCMQKTIGMSFVQYLNYYRLSKAKKW
                     LAETNETIEAIAKRAGIEDGAYFSRLFKKIEGVTPTEYRRTVHRM"
     CDS             complement(6174..7526)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Alpha-amylase"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg478_8"
                     /sec_met_domain="Alpha-amylase (E-value: 2.2e-94, bitscore:
                     305.8, seeds: 19, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="QIFPERFANGNPSLNPEGTLPWGSEEPTPTSFFGGDFEGIIQHLD
                     YLVELGINGIYFTPIFYAPSNHKYDTIDYFEIDPHFGDKQTFKKLVDLCHQKGIRVMLD
                     AVFNHCGYYFAPFQDVLKNGNNSKYKDWFHIHEFPLRTVPRPNYDTFAFVEQMPKLNTE
                     NPEVKQYLLDVATYWIREFDIDGWRLDVANEVDHQFWREFRQAVKAIKPDVYILGEIWH
                     DAMPWLRGDQFDAVMNYPFTNGVISFFAKDEIRAEQFANIIMNVLHSYPANVNEVAFNL
                     LGSHDTPRILTTCNNDVRKVKLLFLFQLSFTGSPCIYYGDEIGMTGGQDPGCRKCMIWD
                     ESKQNRDILEHVKKLISLRKTHPALGSRGNITFIDANNETNHLIYTKTYKHETILVMIN
                     NNNKNIEVTVPLSLKGKRLTNLWTNEQFAVEAKTLRANIPPYGFLLYQVEN"
ORIGIN
        1 aaagctagca ataatcataa gtaagccgat caccggaata acggtcatat attgacctat
       61 tccatactcc ccaagttcct ttgccaaagt taaattaatt aaaaactcaa tacattcact
      121 aataaaggct gccccagcta aaatgattgt tcccttcaaa aatgaattca tctattcgtc
      181 tcctttaata tcaatcacct agaaatcctt ccactacttc ataattatga gacgagcctt
      241 ccaattatta ttaactttta ttaacacagt ttctatagtg taaactacaa caaacatgtg
      301 ttcttactct gattcttata taactacaca ataaataact tttcaaaaaa tatcacattt
      361 ttgtcataaa ataggacttc aaacttagtc atacaatgtt atactaaaca cataaaaagt
      421 ttgctcagta tttcacaata ctgaactgta ttagttattg tgtatataac tgttacaata
      481 ttatacattt aaacttccaa acttaagcaa ctacaatttc ctttttagga ggtcatttta
      541 tgaaacaatg ggaaggtttt aaacctggaa aatggcaaga aaaagttgat gttcgtgatt
      601 ttatattaaa aaatgtaacc ccatatgaag gtgatgaaag ctttttagaa ggtcctactg
      661 aagcgacaac taatctgtgg gagcaagtaa tggaactaaa taaaaaggaa cgtgaagcag
      721 gtggcatttt agatgcagat actaaagttg tttctaccat cacttctcac ggtcctggat
      781 atttaaacaa agaattagaa cagattgtcg gatttcaaac agacaaacca tttaaacgtt
      841 cattaatgcc tttcggtgga attcgaatgg caaaacaagc gttagaagct tacggatttg
      901 aattagatcc tgaagtggaa catattttca cagaatggcg caaaacccat aaccaaggtg
      961 tatttgatgc gtatacacca gaaatgagaa gagctcgttc tgccggtgtt attaccggac
     1021 ttcctgatgc atacggtcgt ggtcgtatta tcggagatta tcgccgtgtt gcgttatacg
     1081 gtgttgattt cttaattcgc caaaaagaag aagataaaaa ctacatcggt agtcggacaa
     1141 tgactcctga tatcattcgt gaccgtgaag aacactcaga acaaattcgt gcacttcacg
     1201 aactaaaaca aatggcagct tcttacggtt ttgatattag tgaaccggca agtaacgcaa
     1261 aagaagcaat ccaatggtta tacttcggct atctagctgc aattaaagaa cagaacggtg
     1321 cggcaatgag tttaggacgg gtatctactt tcctagatat atacattgaa cgtgacttaa
     1381 aaaatggtac attgactgaa agagaagctc aggaacttgt cgatcatttc gtcatgaaac
     1441 ttcgtttagt taaattcgct agaacaccag aatataacga attatatagt ggcgatccaa
     1501 cttgggtaac agaatccatt ggtggtatgg cccttgacgg acggacatta gttacgaaaa
     1561 actctttccg tttcttaaat acattagata acttaggtcc agcaccagaa ccaaacttaa
     1621 caattctctg gtcaactaaa ttaccagaag gattcaaaaa atacgctgcg aaaatgtcca
     1681 ttaaatcaag cgcaatacaa tacgaaaatg atgatttaat gcgtgacatt tatggtgatg
     1741 attacggaat tgcatgttgt gtatcagcta tgcgaattgg taagcagatg caattcttcg
     1801 gtgcccgcgt aaacgtcgca aaagcattac tttatgcaat caacggcggt gtcgacgaaa
     1861 tgaaaaaagt tcaagtcggt ccaaaatttg caccaattac ttccgaatat cttgaatatg
     1921 aagaagttgt tgataaattt gatgcgatgc ttgactggtt atgtgaacta tatgtcaaca
     1981 ctttaaacgt catccattac atgcatgata aatatgcata cgaacgaatt gaaatggcgt
     2041 tacatgaccg tgaagtatta cgtacaatgg cctgtggtat cgctggttta tctgttgcag
     2101 ctgactcttt aagtgcaatt aaacatgcga aagtaaaaac aattcgtgat gaaaatggca
     2161 ttgttgttga ttatgaagtt gaaggtgact tccctaaata tggtaacaat gatgaccgcg
     2221 ttgacgaaat cgctgttaat attgttaaaa aagtaatgaa aaacattcgt aaacatcaaa
     2281 cttatcgtaa tgcagtacca actcagtctg tacttacaat tacttcaaac gttgtatacg
     2341 gtaagaaaac tggtaataca ccagatggac gtgaagctgg taaaccattt gcaccaggtg
     2401 caaacccaat gcatggtcgc gatacaaaag gtgcattggc atccttaaca tctgttgcca
     2461 aattaccata tgaagattca ctagatggaa tctcaaatac atttacaatc acaccgaaag
     2521 caattggtcg tgatgatgaa gcacaagtga acaacttagt aggtatgtta gacggttata
     2581 tgacaaaaga aggtcatcat cttaatgtga acgtattgaa ccgtgacaca ttattagatg
     2641 cgatggatca tcctgaatta tatccacaat taaccattcg tgtttctgga tatgcggtta
     2701 actttattaa attaacccgt gaacaacaaa ttgacgtgat taaccggaca ttccatcaat
     2761 ctatgtaata gtttgattgt taagtatctt tatttctcaa aagtctatta ttaaatttat
     2821 ttcattctcc caatttttat ataaatgaag agaagtcccc cgcttctctt ccccttttaa
     2881 attcgcaagg aggcttttca atggtaacag gtcgaattca ctctattgaa acatttggtg
     2941 cagtagatgg tcccggttta agatatatcg tatttatgca aggatgctcg ctccgctgcc
     3001 tatattgcca taatgcagat acgtggaaaa tgaaaagtgg caaagaaatt accgttcaag
     3061 aaataatcga cgaagtgtta gattatatgc ctttttttga ggcaagtggc ggtggaataa
     3121 ccgtaagtgg cggtgaagct ttattacaaa tcgactttct catcgagtta tttaaagaat
     3181 gtaagcgaca tggtattcat actacccttg atacttcagg cggtcctttc tcccgacgcc
     3241 cgaattactt aaacaaactt gatgagttac ttaagtatac ggatttaatt ttactcgatc
     3301 tcaaacatat tgatagtgag aaacataaat tgttaactgg tatgcataat gagcatatac
     3361 ttgatttcgc aacctattta tctgataaaa aaattcctgt ctggattcgc catgtactcg
     3421 taccaacgat ttctgatttt gatgaagacc tgtaccgcct tgctgaattt attcaaactc
     3481 tggacaatgt tgaaaaagtt gaggttctcc cctaccatca gcttggcgta tataaatggg
     3541 aaaacttagg tttagaatat aaattaaaaa atgtagatcc ccctacacaa gaacggatta
     3601 aaaatgctga aaaaatttta ggtgctagtt aaatattgaa agttaaacgt cgcttccttt
     3661 ctatatagat tttttaactt attaatatga ctctggctcg ttttaacaaa aagctagttt
     3721 tgaaatgatg aattatcatt ctgaagctag cttttgttta tttttatgag cgaatttcct
     3781 tgttttgtga gcgatttggt caattttatg agcgaatacc ctgtttttat gagcgacttt
     3841 ttaaatgtat gagcgaactg gtattctacg agcgttttcg acttgtctat gagcgatttc
     3901 tcttgtccta tgagcgaaaa ttatttataa aaattaatcc ttgtaatcta tgagcgattt
     3961 tttgacctgt ttgatcgact tttcaacccc tatgagcgaa tttaccccac tataagaatg
     4021 gtgcctccct gtttatggaa ttatcccctt cataaaaaat gtatttttca tttcatcttt
     4081 ttacctttaa gatactatcc atatcttccc gcatacaata gttaaaaatg aaaggaagag
     4141 gtatatgccc atatcatttc ctgtcgtcat tcaaacaagg aaggtaagtg atggaccgaa
     4201 aaaatatgtt tattacccgc aaattattga attaaaaaac aaacaagttg aacaacatat
     4261 caatcgaaca atcgtgcatc atacacaaca attaatgaat caacagttcg gaaatatgcc
     4321 atccatcatt gtcgaaatgg ttggttcctt tgaaattaaa aacaatcagc gggatatttt
     4381 aagccttact ttatcaaatt atgcttatcc tgaccgagca gcacacggta tgacatattt
     4441 agattcatta acctttaatg ttgaaactgg aaaacaatac gcgttaaagg atttatttaa
     4501 acctggaagt gattatattg aaaggatctc aaatataata aaaggacaaa ttaaacagcg
     4561 ggatttacct gtgattaccg aatttacaac aataaagcca gatcaagatt attatattgc
     4621 ggataaagca cttgtcatct attttcaact gtacgattta gtgccatacg catggggatt
     4681 tccaatgttt ccaatttccg tttacgatat tcaagatatc attgacgaaa acggacctct
     4741 tggcaaaatg ttagctggta attaaagtgg tacagaggaa agtggtacag agggacgggt
     4801 tcggtgtttc tattttgata ttgaaacacc tgacctgccc ctgtgtttcc cttttgcatt
     4861 atcataccta tgtatttcaa tttgttcatc tatgaaactt ttttattttt aaagcgtaat
     4921 gaaatgtaaa ccctttaaaa tttcattagt gaggtcggtg tagttgaaaa aaattatagg
     4981 attgatattt tcagtcgtcc taatcgttag cttaactgca tgtagtggta atgaagttaa
     5041 gaatacaatt aatgaaaaaa acaagcaaac cacagaagtt acaggagagc aagaaaggca
     5101 agtcgatatt cctatcgaat tttagcggga aactttgaag gagtaataga tcccggatat
     5161 gatgaaatgg caaagaacga agaacggata atgaaaatgg catttttgtc cgtttcaata
     5221 ataaagaaaa gatagatttg atttgtatat ttccgacatg tgaaaggatg tttggaatgg
     5281 aatacataac actttctttt ccgccgtttc cgatttttat taaaggggca gaaagcgtat
     5341 ttccaaaagg acaaaaacat tttcgacgga cgttttctgt gtttgaattg ttatatgtga
     5401 aaagggatgc ttatatatga cagaagaaaa caaggaattt gctgtaaaag aaggaaaata
     5461 tcttattctt attccgggaa gagagcatta cgggcattat ccttgcacgg aagaaacaca
     5521 actagtttgg cttcatttta cgatcgaagg aggatatgac attgttccaa aacagtcgtt
     5581 aagctgacaa aatgtcattg agagggaagc aacatatgtc aagtcggttc aatatcgttt
     5641 ttggatacgg caatatgcga aagtaaagca aagagaacgc atcgaacaat tgcttcaaca
     5701 attagtcgag ctgaatgagg aaagagatcc agatcgttca ttaaagcaat tactctattt
     5761 tgttgaattc atttggcaat tgcaaaaaca ggagctgcat attccaagcg cttctgaaaa
     5821 agtgtgtgcc gaagcggttg cttacatcga acggcattat gctgaaccga ttacgattca
     5881 gcagctcgcc caatcattac gatttcatcc tgactacatt acccgttgca tgcaaaaaac
     5941 aattggcatg agttttgtgc aatatttgaa ttattatcgc ttgtcaaaag caaaaaaatg
     6001 gttggcagaa acgaatgaaa cgattgaagc gattgccaag agagcaggaa ttgaagatgg
     6061 cgcctatttt tcaagactat tcaaaaaaat agaaggagtt acaccgacag aatatcgccg
     6121 tactgtgcat cggatgtaaa aaaagcgaga ggatacttcc ttctcgcctt ttatcaattt
     6181 tctacttgat agagaagaaa accgtatgga ggaatgtttg cacgtaatgt ttttgcttct
     6241 acagcaaatt gctcgtttgt ccataagttc gttagccgct ttcctttaag agacaatggg
     6301 accgtgactt caatattttt gttgttattg ttgatcataa ctaaaatcgt ttcgtgcttg
     6361 tatgttttcg tatatattaa atgatttgtt tcattatttg catcaataaa tgtaatattt
     6421 cctcgacttc caagagcagg atgagtcttg cgaagcgaaa tgagtttttt cacatgttct
     6481 aatatatcac ggttttgttt actttcatcc caaatcatac atttgcggca tcctggatct
     6541 tgcccacctg tcataccaat ttcgtcacca tagtatatgc atggagaacc ggtaaaagag
     6601 agttggaata aaaatagcag tttgactttt cgtacgtcat tgttacatgt cgttaaaatt
     6661 cttggtgtat cgtgactacc aagcaaatta aaagcgactt cattgacgtt tgctgggtaa
     6721 gaatgcagaa cgttcataat tatattagca aattgttcag cacggatttc gtctttggcg
     6781 aaaaagctga taacgccatt cgtaaatgga taattcatga cagcatcaaa ttgatcgcct
     6841 cgaagccatg gcattgcatc gtgccaaatt tctccgagaa tataaacatc cggtttgatt
     6901 gctttcaccg cttgacggaa ttctcgccaa aattggtggt ctacttcgtt tgctacatct
     6961 aatcgccatc catcgatgtc aaactcgcga atccaatatg ttgctacatc taataaatac
     7021 tgctttacct caggattttc cgtatttaac tttggcattt gctcaacaaa tgcgaacgta
     7081 tcatagtttg gtcttgggac tgtccgtaac ggaaattcgt gaatatgaaa ccaatcctta
     7141 tattttgaat tattaccatt ttttagtaca tcttgaaacg gtgcaaaata gtaaccgcaa
     7201 tgattaaata ctgcatctag catcactctt attccttttt gatggcaaag atcaacaagt
     7261 tttttaaatg tttgtttatc tccaaaatgt ggatcgattt caaaataatc tatcgtatca
     7321 tatttatgat tagatggcgc atagaaaata ggagtaaaat aaatgccgtt aataccgagc
     7381 tcaacaagat aatcaaggtg ttgtataatc ccttcaaaat cgccgccaaa aaaacttgtt
     7441 ggtgtcggtt cttcgcttcc ccaaggtagc gttccctctg gattcaaaga cggatttcca
     7501 tttgcaaagc gctccgggaa aatttga
//
