GCA_905311035.1 - 13 region(s)

Identified secondary metabolite regions using strictness 'relaxed'

CAJNPR010000001 (original name was: CAJNPR010000001.1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1.1	T3PKS	888,781	929,878
Region 1.2	terpene	978,325	1,000,256
Region 1.3	NRPS,betalactone	1,072,834	1,150,584	fengycin	NRP	100%

CAJNPR010000002 (original name was: CAJNPR010000002.1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 2.1	terpene	197,830	218,633
Region 2.2	transAT-PKS,PKS-like,T3PKS,NRPS	811,582	918,710	bacillaene	Polyketide + NRP	100%

CAJNPR010000003 (original name was: CAJNPR010000003.1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 3.1	CDPS	38,897	59,643
Region 3.2	sactipeptide	281,545	303,156	subtilosin A	RiPP:Thiopeptide	100%
Region 3.3	other	309,941	351,359	bacilysin	Other	100%

CAJNPR010000004 (original name was: CAJNPR010000004.1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 4.1	NRPS	82,111	129,259	bacillibactin	NRP	100%

CAJNPR010000006 (original name was: CAJNPR010000006.1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 6.1	NRPS	1	25,312	surfactin	NRP:Lipopeptide	43%

CAJNPR010000007 (original name was: CAJNPR010000007.1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 7.1	lanthipeptide-class-i	28,829	53,286	subtilomycin	RiPP:Lanthipeptide	100%
Region 7.2	NRPS	179,352	207,522	surfactin	NRP:Lipopeptide	43%

CAJNPR010000013 (original name was: CAJNPR010000013.1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 13.1	NRPS	1	9,144	surfactin	NRP:Lipopeptide	8%

Region	Type	From	To	Most similar known cluster		Similarity
Region 1.1	T3PKS	888,781	929,878
Region 1.2	terpene	978,325	1,000,256
Region 1.3	NRPS,betalactone	1,072,834	1,150,584	fengycin	NRP	100%
Region 2.1	terpene	197,830	218,633
Region 2.2	transAT-PKS,PKS-like,T3PKS,NRPS	811,582	918,710	bacillaene	Polyketide + NRP	100%
Region 3.1	CDPS	38,897	59,643
Region 3.2	sactipeptide	281,545	303,156	subtilosin A	RiPP:Thiopeptide	100%
Region 3.3	other	309,941	351,359	bacilysin	Other	100%
Region 4.1	NRPS	82,111	129,259	bacillibactin	NRP	100%
Region 6.1	NRPS	1	25,312	surfactin	NRP:Lipopeptide	43%
Region 7.1	lanthipeptide-class-i	28,829	53,286	subtilomycin	RiPP:Lanthipeptide	100%
Region 7.2	NRPS	179,352	207,522	surfactin	NRP:Lipopeptide	43%
Region 13.1	NRPS	1	9,144	surfactin	NRP:Lipopeptide	8%

Compact view

c00001_CAJNPR0.. - Region 1 - T3PKS

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Location: 888,781 - 929,878 nt. (total: 41,098 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

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KnownClusterBlast

Similar known gene clusters

No matches found.

c00001_CAJNPR0.. - Region 2 - terpene

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Location: 978,325 - 1,000,256 nt. (total: 21,932 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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core biosynthetic genes

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KnownClusterBlast

Similar known gene clusters

No matches found.

c00001_CAJNPR0.. - Region 3 - NRPS,betalactone

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Location: 1,072,834 - 1,150,584 nt. (total: 77,751 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))
betalactone: (HMGL-like and AMP-binding)

Legend:

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 3, location 1072833 - 1150584:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (glu - D-orn) + (tyr - D-thr) + (glu - D-val) + (pro - glu - D-tyr) + (ile)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg1_1192

Search NORINE for peptide: strict or relaxed

AMP-binding (463..864): glu

NRPSPredictor2: glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: glu, gln
Single AA prediction:: glu

Stachelhaus prediction details:

Stachelhaus sequence:: DAWHFGGVDK
Nearest Stachelhaus code:: glu
Stachelhaus code match:: 100% (strong)

AMP-binding (1503..1908): orn

NRPSPredictor2: orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: orn, lys, arg
Small clusters prediction:: orn, horn
Single AA prediction:: orn

Stachelhaus prediction details:

Stachelhaus sequence:: DVGEIGSIDK
Nearest Stachelhaus code:: orn
Stachelhaus code match:: 100% (strong)

ctg1_1193

Search NORINE for peptide: strict or relaxed

AMP-binding (474..865): tyr

NRPSPredictor2: tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: phe, trp, phg, tyr, bht
Small clusters prediction:: tyr, bht
Single AA prediction:: tyr

Stachelhaus prediction details:

Stachelhaus sequence:: DGTITAEVAK
Nearest Stachelhaus code:: tyr
Stachelhaus code match:: 100% (strong)

AMP-binding (1505..1903): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

ctg1_1194

Search NORINE for peptide: strict or relaxed

AMP-binding (469..870): glu

NRPSPredictor2: glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: glu, gln
Single AA prediction:: glu

Stachelhaus prediction details:

Stachelhaus sequence:: DAWHFGGVDK
Nearest Stachelhaus code:: glu
Stachelhaus code match:: 100% (strong)

AMP-binding (1510..1903): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: val

Stachelhaus prediction details:

Stachelhaus sequence:: DVFWIGGTFK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 100% (strong)

ctg1_1195

Search NORINE for peptide: strict or relaxed

AMP-binding (468..865): pro

NRPSPredictor2: pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: pro, pip
Small clusters prediction:: pro
Single AA prediction:: pro

Stachelhaus prediction details:

Stachelhaus sequence:: DVQFIAHVVK
Nearest Stachelhaus code:: pro
Stachelhaus code match:: 100% (strong)

AMP-binding (1499..1900): glu

NRPSPredictor2: glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: glu, gln
Single AA prediction:: glu

Stachelhaus prediction details:

Stachelhaus sequence:: DAWHFGSVEK
Nearest Stachelhaus code:: glu
Stachelhaus code match:: 100% (strong)

AMP-binding (2538..2932): tyr

NRPSPredictor2: tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: phe, trp, phg, tyr, bht
Small clusters prediction:: tyr, bht
Single AA prediction:: tyr

Stachelhaus prediction details:

Stachelhaus sequence:: DGTITAEVAK
Nearest Stachelhaus code:: tyr
Stachelhaus code match:: 100% (strong)

ctg1_1196

Search NORINE for peptide: strict or relaxed

AMP-binding (474..867): ile

NRPSPredictor2: ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: ile

Stachelhaus prediction details:

Stachelhaus sequence:: DAFFYGITFK
Nearest Stachelhaus code:: ile
Stachelhaus code match:: 100% (strong)

ctg1_1201

Search NORINE for peptide: strict or relaxed

AMP-binding (13..445): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: N/A
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: gclhIAApvK
Nearest Stachelhaus code:: phe
Stachelhaus code match:: 40% (weak)

c00002_CAJNPR0.. - Region 1 - terpene

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Location: 197,830 - 218,633 nt. (total: 20,804 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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core biosynthetic genes

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KnownClusterBlast

Similar known gene clusters

No matches found.

c00002_CAJNPR0.. - Region 2 - NRPS,PKS-like,T3PKS,transAT-PKS

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Location: 811,582 - 918,710 nt. (total: 107,129 nt) Show pHMM detection rules used

transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))
PKS-like: (t2pks2 or ksIII)
T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)
NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 2, location 811581 - 918710:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (gly - ccmal - ohmal - mal) + (mal - ccmal - mal - ohmal - mal) + (mal - ccmal - ohmal - mal) + (X - ccmal - ccmal - ccmal) + (ccmal - mal - mal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 811581 - 902176:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (gly - ccmal - ohmal - mal) + (mal - ccmal - mal - ohmal - mal) + (mal - ccmal - ohmal - mal) + (X - ccmal - ccmal - ccmal) + (ccmal - mal - mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

c00003_CAJNPR0.. - Region 1 - CDPS

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Location: 38,897 - 59,643 nt. (total: 20,747 nt) Show pHMM detection rules used

CDPS: (CDPS or cycdipepsynth)

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KnownClusterBlast

Similar known gene clusters

No matches found.

c00003_CAJNPR0.. - Region 2 - sactipeptide

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Location: 281,545 - 303,156 nt. (total: 21,612 nt) Show pHMM detection rules used

sactipeptide: (subtilosin or thuricin or TIGR04404 or Subtilosin_A or skfc)

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core biosynthetic genes

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KnownClusterBlast

Sactipeptides

Sactipeptide predictions

Putative Sactipeptide(s)

ctg3_290 leader / core peptide: MKKAVIVENK - GCATCSIGAACLVDGPIPDFEIAGATGLFGLWG

Sactipeptides

Core peptide predictions

ctg3_290: Putative sactipeptide; RODEO score: 33.0; Core sequence, approximate: GCATCSIGAACLVDGPIPDFEIAGATGLFGLWG

c00003_CAJNPR0.. - Region 3 - other

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Location: 309,941 - 351,359 nt. (total: 41,419 nt) Show pHMM detection rules used

other: (LmbU or Neocarzinostat or fom1 or bcpB or frbD or mitE or vlmB or prnB or CaiA or bacilysin or orf2_PTase)

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KnownClusterBlast

Similar known gene clusters

c00004_CAJNPR0.. - Region 1 - NRPS

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Location: 82,111 - 129,259 nt. (total: 47,149 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 82110 - 129259:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (gly - thr)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

c00006_CAJNPR0.. - Region 1 - NRPS

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Location: 1 - 25,312 nt. (total: 25,312 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

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Gene details

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 25312:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (leu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

c00007_CAJNPR0.. - Region 1 - lanthipeptide-class-i

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Location: 28,829 - 53,286 nt. (total: 24,458 nt) Show pHMM detection rules used

lanthipeptide-class-i: ((LANC_like and (Lant_dehydr_N or Lant_dehydr_C)) and not (YcaO or TIGR03882))

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Lanthipeptides

KnownClusterBlast

Lanthipeptide predictions

Lanthipeptide(s) for ctg7_36 - Putative Class I

ctg7_34 leader / core peptide: MEKNNIFDLDINKKMESTSEVSAQ - DhbWADhbIGKDhbIVQDhaVKKCRDhbFDhbCGCDhaLGDhaCDhaNCN

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Core peptide predictions

ctg7_34 - Class I

Cleavage pHMM score: 14.10

RODEO score: 24

Monoisotopic mass: 3233.6 Da

Molecular weight: 3235.8 Da

Number of bridges: 5

Alternative weights: (assuming N unmodified Ser/Thr residues); 3253.8 Da N = 1; 3271.8 Da N = 2; 3289.8 Da N = 3; 3307.8 Da N = 4

c00007_CAJNPR0.. - Region 2 - NRPS

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Location: 179,352 - 207,522 nt. (total: 28,171 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

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Gene details

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 2, location 179351 - 207522:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (glu - leu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

c00013_CAJNPR0.. - Region 1 - NRPS

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Location: 1 - 9,144 nt. (total: 9,144 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

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regulatory genes

other genes

resistance

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Gene details

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 9144:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (val - asp)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form