GCA_905312035.1 - 10 region(s)

Identified secondary metabolite regions using strictness 'relaxed'

CAJNQA010000002 (original name was: CAJNQA010000002.1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 2.1	sactipeptide	41,991	63,602	subtilosin A	RiPP:Thiopeptide	100%

CAJNQA010000003 (original name was: CAJNQA010000003.1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 3.1	RRE-containing	17,569	39,719	subtilin	RiPP:Lanthipeptide	76%

CAJNQA010000008 (original name was: CAJNQA010000008.1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 8.1	NRPS	78,972	117,259	bacillibactin	NRP	100%

CAJNQA010000009 (original name was: CAJNQA010000009.1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 9.1	NRPS	30,679	96,070	surfactin	NRP:Lipopeptide	82%

CAJNQA010000010 (original name was: CAJNQA010000010.1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 10.1	T3PKS	55,751	91,227

CAJNQA010000014 (original name was: CAJNQA010000014.1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 14.1	lanthipeptide-class-iii	53,843	75,370

CAJNQA010000015 (original name was: CAJNQA010000015.1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 15.1	NRPS-like,betalactone,NRPS	10,579	55,157	fengycin	NRP	100%

CAJNQA010000029 (original name was: CAJNQA010000029.1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 29.1	RiPP-like	25,652	37,676

CAJNQA010000033 (original name was: CAJNQA010000033.1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 33.1	terpene	3,867	24,673

CAJNQA010000053 (original name was: CAJNQA010000053.1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 53.1	terpene	1,470	22,174

No secondary metabolite regions were found in these records:: CAJNQA010000001 (original name was: CAJNQA010000001.1); CAJNQA010000004 (original name was: CAJNQA010000004.1); CAJNQA010000005 (original name was: CAJNQA010000005.1); CAJNQA010000006 (original name was: CAJNQA010000006.1); CAJNQA010000007 (original name was: CAJNQA010000007.1); CAJNQA010000011 (original name was: CAJNQA010000011.1); CAJNQA010000012 (original name was: CAJNQA010000012.1); CAJNQA010000013 (original name was: CAJNQA010000013.1); CAJNQA010000016 (original name was: CAJNQA010000016.1); CAJNQA010000017 (original name was: CAJNQA010000017.1); CAJNQA010000018 (original name was: CAJNQA010000018.1); CAJNQA010000019 (original name was: CAJNQA010000019.1); CAJNQA010000020 (original name was: CAJNQA010000020.1); CAJNQA010000021 (original name was: CAJNQA010000021.1); CAJNQA010000022 (original name was: CAJNQA010000022.1); CAJNQA010000023 (original name was: CAJNQA010000023.1); CAJNQA010000024 (original name was: CAJNQA010000024.1); CAJNQA010000025 (original name was: CAJNQA010000025.1); CAJNQA010000026 (original name was: CAJNQA010000026.1); CAJNQA010000027 (original name was: CAJNQA010000027.1); CAJNQA010000028 (original name was: CAJNQA010000028.1); CAJNQA010000030 (original name was: CAJNQA010000030.1); CAJNQA010000031 (original name was: CAJNQA010000031.1); CAJNQA010000032 (original name was: CAJNQA010000032.1); CAJNQA010000034 (original name was: CAJNQA010000034.1); CAJNQA010000035 (original name was: CAJNQA010000035.1); CAJNQA010000036 (original name was: CAJNQA010000036.1); CAJNQA010000037 (original name was: CAJNQA010000037.1); CAJNQA010000038 (original name was: CAJNQA010000038.1); CAJNQA010000039 (original name was: CAJNQA010000039.1); CAJNQA010000040 (original name was: CAJNQA010000040.1); CAJNQA010000041 (original name was: CAJNQA010000041.1); CAJNQA010000042 (original name was: CAJNQA010000042.1); CAJNQA010000043 (original name was: CAJNQA010000043.1); CAJNQA010000044 (original name was: CAJNQA010000044.1); CAJNQA010000045 (original name was: CAJNQA010000045.1); CAJNQA010000046 (original name was: CAJNQA010000046.1); CAJNQA010000047 (original name was: CAJNQA010000047.1); CAJNQA010000048 (original name was: CAJNQA010000048.1); CAJNQA010000049 (original name was: CAJNQA010000049.1); CAJNQA010000050 (original name was: CAJNQA010000050.1); CAJNQA010000051 (original name was: CAJNQA010000051.1); CAJNQA010000052 (original name was: CAJNQA010000052.1); CAJNQA010000054 (original name was: CAJNQA010000054.1); CAJNQA010000055 (original name was: CAJNQA010000055.1); CAJNQA010000056 (original name was: CAJNQA010000056.1); CAJNQA010000057 (original name was: CAJNQA010000057.1); CAJNQA010000058 (original name was: CAJNQA010000058.1); CAJNQA010000059 (original name was: CAJNQA010000059.1); CAJNQA010000060 (original name was: CAJNQA010000060.1); CAJNQA010000061 (original name was: CAJNQA010000061.1); CAJNQA010000062 (original name was: CAJNQA010000062.1); CAJNQA010000063 (original name was: CAJNQA010000063.1); CAJNQA010000064 (original name was: CAJNQA010000064.1); CAJNQA010000065 (original name was: CAJNQA010000065.1); CAJNQA010000066 (original name was: CAJNQA010000066.1); CAJNQA010000067 (original name was: CAJNQA010000067.1); CAJNQA010000068 (original name was: CAJNQA010000068.1); CAJNQA010000069 (original name was: CAJNQA010000069.1); CAJNQA010000070 (original name was: CAJNQA010000070.1); CAJNQA010000071 (original name was: CAJNQA010000071.1); CAJNQA010000072 (original name was: CAJNQA010000072.1); CAJNQA010000073 (original name was: CAJNQA010000073.1); CAJNQA010000074 (original name was: CAJNQA010000074.1); CAJNQA010000075 (original name was: CAJNQA010000075.1); CAJNQA010000076 (original name was: CAJNQA010000076.1); CAJNQA010000077 (original name was: CAJNQA010000077.1); CAJNQA010000078 (original name was: CAJNQA010000078.1); CAJNQA010000079 (original name was: CAJNQA010000079.1); CAJNQA010000080 (original name was: CAJNQA010000080.1); CAJNQA010000081 (original name was: CAJNQA010000081.1); CAJNQA010000082 (original name was: CAJNQA010000082.1); CAJNQA010000083 (original name was: CAJNQA010000083.1); CAJNQA010000084 (original name was: CAJNQA010000084.1); CAJNQA010000085 (original name was: CAJNQA010000085.1); CAJNQA010000086 (original name was: CAJNQA010000086.1); CAJNQA010000087 (original name was: CAJNQA010000087.1); CAJNQA010000088 (original name was: CAJNQA010000088.1); CAJNQA010000089 (original name was: CAJNQA010000089.1); CAJNQA010000090 (original name was: CAJNQA010000090.1); CAJNQA010000091 (original name was: CAJNQA010000091.1); CAJNQA010000092 (original name was: CAJNQA010000092.1); CAJNQA010000093 (original name was: CAJNQA010000093.1); CAJNQA010000094 (original name was: CAJNQA010000094.1); CAJNQA010000095 (original name was: CAJNQA010000095.1); CAJNQA010000096 (original name was: CAJNQA010000096.1); CAJNQA010000097 (original name was: CAJNQA010000097.1); CAJNQA010000098 (original name was: CAJNQA010000098.1); CAJNQA010000099 (original name was: CAJNQA010000099.1); CAJNQA010000100 (original name was: CAJNQA010000100.1); CAJNQA010000101 (original name was: CAJNQA010000101.1); CAJNQA010000102 (original name was: CAJNQA010000102.1); CAJNQA010000103 (original name was: CAJNQA010000103.1); CAJNQA010000104 (original name was: CAJNQA010000104.1); CAJNQA010000105 (original name was: CAJNQA010000105.1); CAJNQA010000106 (original name was: CAJNQA010000106.1); CAJNQA010000107 (original name was: CAJNQA010000107.1); CAJNQA010000108 (original name was: CAJNQA010000108.1); CAJNQA010000109 (original name was: CAJNQA010000109.1); CAJNQA010000110 (original name was: CAJNQA010000110.1); CAJNQA010000111 (original name was: CAJNQA010000111.1); CAJNQA010000112 (original name was: CAJNQA010000112.1); CAJNQA010000113 (original name was: CAJNQA010000113.1); CAJNQA010000114 (original name was: CAJNQA010000114.1)

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c00002_CAJNQA0.. - Region 1 - sactipeptide

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Location: 41,991 - 63,602 nt. (total: 21,612 nt) Show pHMM detection rules used

sactipeptide: (subtilosin or thuricin or TIGR04404 or Subtilosin_A or skfc)

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core biosynthetic genes

additional biosynthetic genes

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KnownClusterBlast

Sactipeptides

Similar known gene clusters

Sactipeptide predictions

Putative Sactipeptide(s)

ctg2_55 leader / core peptide: MKKAVIVENK - GCATCSIGAACLVDGPIPDFEIAGATGLFGLWG

Sactipeptides

Core peptide predictions

ctg2_55: Putative sactipeptide; RODEO score: 33.0; Core sequence, approximate: GCATCSIGAACLVDGPIPDFEIAGATGLFGLWG

c00003_CAJNQA0.. - Region 1 - RRE-containing

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Location: 17,569 - 39,719 nt. (total: 22,151 nt) Show pHMM detection rules used

RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

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core biosynthetic genes

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KnownClusterBlast

Similar known gene clusters

c00008_CAJNQA0.. - Region 1 - NRPS

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Location: 78,972 - 117,259 nt. (total: 38,288 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 78971 - 117259:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (gly - thr)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg8_101

Search NORINE for peptide: strict or relaxed

AMP-binding (463..861): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGLIWK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 100% (strong)

AMP-binding (1519..1935): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

ctg8_103

Search NORINE for peptide: strict or relaxed

AMP-binding (31..435): dhb

NRPSPredictor2: dhb

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: dhb, sal
Small clusters prediction:: dhb, sal
Single AA prediction:: dhb

Stachelhaus prediction details:

Stachelhaus sequence:: PLPAQGVVNK
Nearest Stachelhaus code:: dhb
Stachelhaus code match:: 100% (strong)

c00009_CAJNQA0.. - Region 1 - NRPS

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Location: 30,679 - 96,070 nt. (total: 65,392 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

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Gene details

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 30678 - 96070:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (glu - leu - D-leu) + (val - asp - D-leu) + (leu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg9_58

Search NORINE for peptide: strict or relaxed

AMP-binding (469..867): leu

NRPSPredictor2: leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: leu

Stachelhaus prediction details:

Stachelhaus sequence:: DAFFLGCVFK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 100% (strong)

ctg9_59

Search NORINE for peptide: strict or relaxed

AMP-binding (470..864): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: val

Stachelhaus prediction details:

Stachelhaus sequence:: DAFWIGGTFK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 100% (strong)

AMP-binding (1501..1904): asp

NRPSPredictor2: asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asp

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKVGHVGK
Nearest Stachelhaus code:: asp
Stachelhaus code match:: 100% (strong)

AMP-binding (2546..2934): leu

NRPSPredictor2: leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: leu

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFLGNVVK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 100% (strong)

ctg9_60

Search NORINE for peptide: strict or relaxed

AMP-binding (466..867): glu

NRPSPredictor2: glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: glu, gln
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAKDLGVVDK
Nearest Stachelhaus code:: glu
Stachelhaus code match:: 100% (strong)

AMP-binding (1510..1908): leu

NRPSPredictor2: leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: leu

Stachelhaus prediction details:

Stachelhaus sequence:: DAFMMGMVFK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 100% (strong)

AMP-binding (2550..2938): leu

NRPSPredictor2: leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: leu

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFLGNVVK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 100% (strong)

c00010_CAJNQA0.. - Region 1 - T3PKS

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Location: 55,751 - 91,227 nt. (total: 35,477 nt) Show pHMM detection rules used

Region on contig edge.

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

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KnownClusterBlast

Similar known gene clusters

No matches found.

c00014_CAJNQA0.. - Region 1 - lanthipeptide-class-iii

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Location: 53,843 - 75,370 nt. (total: 21,528 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-iii: (micKC and not PKS_KS)

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Lanthipeptides

KnownClusterBlast

Lanthipeptide predictions

Lanthipeptide(s) for ctg14_72 - Putative Class III

ctg14_78 leader / core peptide: MSRGKQISNILKLQSLESGPVYTAGAT - GDhbVEGQDhaLIDhaPADhaIAFCLDhbPLPVDhaDhbMDhaAAFCNN

Lanthipeptide(s) for ctg14_79 - Putative Class III

ctg14_78 leader / core peptide: MSRGKQISNILKLQSLESGPVYTAGAT - GDhbVEGQDhaLIDhaPADhaIAFCLDhbPLPVDhaDhbMDhaAAFCNN

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Similar known gene clusters

No matches found.

Lanthipeptides

Core peptide predictions

ctg14_78 - Class III

Cleavage pHMM score: -3.30

RODEO score: 0

Monoisotopic mass: 3181.6 Da

Molecular weight: 3183.6 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 3201.7 Da N = 1; 3219.7 Da N = 2; 3237.7 Da N = 3; 3255.7 Da N = 4; 3273.7 Da N = 5; 3291.8 Da N = 6

ctg14_78 - Class III

Cleavage pHMM score: -3.30

RODEO score: 0

Monoisotopic mass: 3181.6 Da

Molecular weight: 3183.6 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 3201.7 Da N = 1; 3219.7 Da N = 2; 3237.7 Da N = 3; 3255.7 Da N = 4; 3273.7 Da N = 5; 3291.8 Da N = 6

c00015_CAJNQA0.. - Region 1 - NRPS,NRPS-like,betalactone

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Location: 10,579 - 55,157 nt. (total: 44,579 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))
betalactone: (HMGL-like and AMP-binding)
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

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Gene details

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NRPS/PKS domains

KnownClusterBlast

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 10578 - 55157:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ile)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 10578 - 49778:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ile)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 13195 - 55157:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ile)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg15_27

Search NORINE for peptide: strict or relaxed

AMP-binding (13..445): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: N/A
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: gclhIAApvK
Nearest Stachelhaus code:: phe
Stachelhaus code match:: 40% (weak)

ctg15_33

Search NORINE for peptide: strict or relaxed

AMP-binding (1..335): ile

NRPSPredictor2: ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: ile

Stachelhaus prediction details:

Stachelhaus sequence:: DAFFYGITFK
Nearest Stachelhaus code:: ile
Stachelhaus code match:: 100% (strong)

ctg15_38

Search NORINE for peptide: strict or relaxed

AMP-binding (15..246): X

NRPSPredictor2: phe, trp, phg, tyr, bht

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: phe, trp, phg, tyr, bht
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DGT------K
Nearest Stachelhaus code:: tyr
Stachelhaus code match:: 40% (weak)

c00029_CAJNQA0.. - Region 1 - RiPP-like

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Location: 25,652 - 37,676 nt. (total: 12,025 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193)

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core biosynthetic genes

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KnownClusterBlast

Similar known gene clusters

No matches found.

c00033_CAJNQA0.. - Region 1 - terpene

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Location: 3,867 - 24,673 nt. (total: 20,807 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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core biosynthetic genes

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KnownClusterBlast

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No matches found.

c00053_CAJNQA0.. - Region 1 - terpene

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Location: 1,470 - 22,174 nt. (total: 20,705 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

Legend:

core biosynthetic genes

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KnownClusterBlast

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No matches found.