Probiotic potential risk score

ARGs (CARD)	VFs (VFDB)	PGs (PHI-base)	PPRS
0	18	15	23.43

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

ARO accession	ARO name	Identity	Coverage	CARD name	Accession no.
No hit found

RGI v6.0.0 (Database: CARD Variants v4.0.0, Jul. 2022 release)

ResFinder

Resistance gene	Identity	Coverage	Phenotype	Accession no.
No hit found

ResFinder v4.0 (Database: resfinder_db, May 2022 release)

AMRFinderPlus

Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v3.10.42 (Database: Oct. 2022 release)

Virulence factors

Virulence Factor Database (VFDB)

Gene ID	Gene name	Identity	Coverage	e-value	VF name	VF category	Accession no.
VFG005322	cbpD	100.00	82.14	0.0	Autolysin	Exoenzyme	WP_011226733
VFG006046	STR_RS05880	99.83	100.00	0.0	Capsule	Immune modulation	WP_011226149
VFG005191	fbp54	99.76	100.00	0.0	FBPs	Adherence	WP_011681101
VFG006084	STU_RS16245	99.75	100.00	0.0	Capsule	Immune modulation	WP_002948020
VFG006052	rfbA	99.66	100.00	0.0	Capsule	Immune modulation	WP_011226150
VFG006088	STU_RS16255	99.65	100.00	0.0	Capsule	Immune modulation	WP_041828290
VFG006078	STU_RS16235	99.53	100.00	0.0	Capsule	Immune modulation	WP_002948022
VFG006074	STU_RS16230	99.44	100.00	0.0	Capsule	Immune modulation	WP_011226325
VFG006022	rfbB	99.43	100.00	0.0	Capsule	Immune modulation	WP_002947383
VFG006082	STU_RS16240	99.38	92.95	0.0	Capsule	Immune modulation	WP_011226326
VFG006081	rfbD	99.35	72.54	0.0	Capsule	Immune modulation	WP_014621820
VFG005986	STR_RS05245	98.93	93.33	0.0	Capsule	Immune modulation	WP_196767671
VFG006058	STU_RS14650	98.76	100.00	0.0	Capsule	Immune modulation	WP_011226049
VFG005974	STR_RS05240	98.23	100.00	0.0	Capsule	Immune modulation	WP_041827042
VFG005976	STER_RS05305	97.96	99.33	0.0	Capsule	Immune modulation	WP_011681174
VFG006090	STU_RS16260	97.83	99.70	0.0	Capsule	Immune modulation	WP_011226330
VFG006000	STER_RS05315	96.97	81.15	0.0	Capsule	Immune modulation	WP_011681176
VFG006047	STU_RS14640	93.25	98.41	0.0	Capsule	Immune modulation	WP_011226047
VFG005555	tig/ropA	98.54	63.55	0.0	Trigger factor	Stress survival	WP_011225339

BLASTN v2.8.1+ (Database: VFDB, Jun. 2022 release)

VirulenceFinder

Database	Virulence factor	Identity	Coverage	Protein function	Accession no.
No hit found

VirulenceFinder v2.0.3 (Database: virulencefinder_db, Dec. 2022 release)

Pathogenic genes

PHI-base

Protein ID	Gene name	Identity	Coverage	e-value	Function	Gene ID
Q1JLQ2	MntE	94.41	99.44	0.0	Manganese Homeostasis	ABF32167
Q97S31	metE	91.72	99.87	0.0	Methionine biosynthesis enzymes	AAK74738
D5AF41	IMPDH	89.65	86.03	0.0	Inosine-5'-monophosphate dehydrogenase	ADE32434
P0C0B9	bglB	88.55	99.33	0.0	Putative phospho--glucosidase	AAK33487
A4VYE0	gidA	86.05	98.75	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	ABP91129
Q97S30	metF	86.01	98.96	0.0	Methionine biosynthesis enzymes	AAK74739
A4VWD1	mnmE	82.98	71.83	0.0	Central tRNA-modifying GTPase	ABP90420
Q1JJJ3	PepO	80.03	99.84	0.0	Endopeptidase	ABF32980
Q97RW0	SP_0676	83.09	84.21	2.29e-168	Transcription facot	AAK74821
Q1J943	prsA2	90.27	79.08	8.41e-167	Ribose-phosphate pyrophosphokinase	ABF36968
Q9S1K2	rr01	84.38	99.12	2.21e-144	Response regulator	CAB54566
P0A4H7	CiaR	84.21	76.00	1.16e-105	Two-component response regulator	AAK74935
U2W9V3	SpxA2	90.91	99.25	2.51e-88	Transcriptional regulator	ERL21461
Q8DQV4	spr0479	86.46	97.96	8.45e-59	essential gene	AAK99283
Q04M32	ilvC (SPD_0406)	94.22	81.23	0.0	Ketol-acid reductoisomerase (NADP(+))	ABJ55451
B5TQV9	PGM	84.08	65.79	0.0	phosphoglucomutase	ACH87041
P67331	spr0084	91.07	68.09	9.74e-154	pathogenitcity related	AAK98888
A4VY17	pdh	85.22	67.85	1.32e-146	Pyruvate dehydrogenase	ABP91006

BLASTP v2.8.1+ (Database: PHI-base v4.14, Nov. 2022 release)

Plasmid

PlasmidFinder

Plasmid	Identity	Query / Template	Accession no.
No hit found

PlasmidFinder 2.0.1 (Database: plasmidfinder_db, Jan. 2023 release)

Prophage

Phigaro

Scaffold	Begin	End	Transposable	Taxonomy	pVOGs
No hit found

Phigaro v2.3.0 (Database: Aug. 2017 release)