Probiotic potential risk score

ARGs (CARD)	VFs (VFDB)	PGs (PHI-base)	PPRS
4	1	4	5.74

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

ARO accession	ARO name	Identity	Coverage	CARD name	Accession no.
ARO:3002816	clbC	99.43	100.00	clbC	AP006627.1
ARO:3002623	ANT(4')-Ia	98.05	100.00	ANT(4')-Ia	EF540343.1
ARO:3000600	Erm(34)	96.80	100.00	Erm(34)	AY234334.1
ARO:3004452	Alkalihalobacillus clausii chloramphenicol acetyltransferase	87.74	94.30	Acla_ACT_CHL	AY238971.1

RGI v6.0.0 (Database: CARD Variants v4.0.0, Jul. 2022 release)

ResFinder

Resistance gene	Identity	Coverage	Phenotype	Accession no.
ant(4')-Ib	98.83	100.00	Aminoglycoside resistance	AJ506108
cat	96.79	98.40	Phenicol resistance	AY238971
erm(34)	96.04	98.46	Macrolide resistance	AY234334

ResFinder v4.0 (Database: resfinder_db, May 2022 release)

AMRFinderPlus

Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
clbB	23S rRNA (adenine(2503)-C(8))-methyltransferase ClbB	BLASTX	99.43	100.00	WP_011245929.1
catA	type A-10 chloramphenicol O-acetyltransferase	BLASTX	99.07	100.00	WP_035203840.1
aadD2	aminoglycoside O-nucleotidyltransferase ANT(4')-Ib	BLASTX	98.05	100.00	WP_035202393.1
erm(34)	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(34)	BLASTX	96.80	100.00	WP_063844504.1
blaBCL	BCL family class A beta-lactamase	BLASTX	91.69	100.00	WP_065825670.1

AMRFinderPlus v3.10.42 (Database: Oct. 2022 release)

Virulence factors

Virulence Factor Database (VFDB)

Gene ID	Gene name	Identity	Coverage	e-value	VF name	VF category	Accession no.
VFG016238	hlyIII	99.54	100.00	0.0	Hemolysin III	Exotoxin	WP_011247375

BLASTN v2.8.1+ (Database: VFDB, Jun. 2022 release)

VirulenceFinder

Database	Virulence factor	Identity	Coverage	Protein function	Accession no.
No hit found

VirulenceFinder v2.0.3 (Database: virulencefinder_db, Dec. 2022 release)

Pathogenic genes

PHI-base

Protein ID	Gene name	Identity	Coverage	e-value	Function	Gene ID
W8TRT9	tufA	87.31	99.24	0.0	Elongation factor Tu	AHM69299
Q724L7	SpoVG	81.48	78.64	6.58e-45	RNA-Binding Protein	AAT02994
P0A355	CspA	86.15	97.01	1.37e-37	Cold shock protein	CAA62903
Q2FH36	msaB	81.25	95.52	2.37e-33	Cold shock protein	ABD22065

BLASTP v2.8.1+ (Database: PHI-base v4.14, Nov. 2022 release)

Plasmid

PlasmidFinder

Plasmid	Identity	Query / Template	Accession no.
No hit found

PlasmidFinder 2.0.1 (Database: plasmidfinder_db, Jan. 2023 release)

Prophage

Phigaro

Scaffold	Begin	End	Transposable	Taxonomy	pVOGs
No hit found

Phigaro v2.3.0 (Database: Aug. 2017 release)