Probiotic potential risk score

ARGs (CARD)	VFs (VFDB)	PGs (PHI-base)	PPRS
1	15	30	33.56

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

ARO accession	ARO name	Identity	Coverage	CARD name	Accession no.
ARO:3007011	mdeA	81.33	99.34	mdeA	NZ_CP044221.1

RGI v6.0.0 (Database: CARD Variants v4.0.0, Jul. 2022 release)

ResFinder

Resistance gene	Identity	Coverage	Phenotype	Accession no.
msr(D)	100.00	100.00	Macrolide, Lincosamide and Streptogramin B resistance	AF274302
mef(A)	99.92	100.00	Macrolide resistance	U83667

ResFinder v4.0 (Database: resfinder_db, May 2022 release)

AMRFinderPlus

Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
mef(A)	macrolide efflux MFS transporter Mef(A)	EXACTX	100.00	100.00	WP_000417519.1
msr(D)	ABC-F type ribosomal protection protein Msr(D)	EXACTX	100.00	100.00	WP_000420313.1

AMRFinderPlus v3.10.42 (Database: Oct. 2022 release)

Virulence factors

Virulence Factor Database (VFDB)

Gene ID	Gene name	Identity	Coverage	e-value	VF name	VF category	Accession no.
VFG006087	STER_RS07100	96.25	99.52	0.0	Capsule	Immune modulation	WP_011681445
VFG006079	STER_RS07085	96.17	98.33	0.0	Capsule	Immune modulation	WP_011681442
VFG006089	rfbD	94.60	100.00	0.0	Capsule	Immune modulation	WP_011681446
VFG005556	tig/ropA	94.16	100.00	0.0	Trigger factor	Stress survival	WP_011680653
VFG006022	rfbB	93.89	100.00	0.0	Capsule	Immune modulation	WP_002947383
VFG006075	STER_RS07075	92.28	100.00	0.0	Capsule	Immune modulation	WP_011681440
VFG006041	rfbA	91.95	100.00	0.0	Capsule	Immune modulation	WP_011681286
VFG005191	fbp54	90.66	99.94	0.0	FBPs	Adherence	WP_011681101
VFG018652	plr/gapA	90.26	100.00	0.0	Streptococcal plasmin receptor/GAPDH	Adherence	WP_006154814
VFG006046	STR_RS05880	89.39	100.00	0.0	Capsule	Immune modulation	WP_011226149
VFG005325	cbpD	87.90	99.76	0.0	Autolysin	Exoenzyme	WP_011680595
VFG005576	eno	87.79	99.39	0.0	Streptococcal enolase	Exoenzyme	WP_000022832
VFG006000	STER_RS05315	83.95	99.59	0.0	Capsule	Immune modulation	WP_011681176
VFG005964	STER_RS05300	83.76	99.78	0.0	Capsule	Immune modulation	WP_011681173
VFG005382	srtA	83.57	99.61	0.0	Sortase A	Exoenzyme	WP_011681303

BLASTN v2.8.1+ (Database: VFDB, Jun. 2022 release)

VirulenceFinder

Database	Virulence factor	Identity	Coverage	Protein function	Accession no.
No hit found

VirulenceFinder v2.0.3 (Database: virulencefinder_db, Dec. 2022 release)

Pathogenic genes

PHI-base

Protein ID	Gene name	Identity	Coverage	e-value	Function	Gene ID
Q54970	spxB	97.29	99.83	0.0	Pyruvate Oxidase Gene	AAB40976
Q1JLQ2	MntE	94.69	99.44	0.0	Manganese Homeostasis	ABF32167
P67331	spr0084	92.88	98.18	0.0	pathogenitcity related	AAK98888
Q97S31	metE	92.66	99.87	0.0	Methionine biosynthesis enzymes	AAK74738
Q1J943	prsA2	91.88	98.46	0.0	Ribose-phosphate pyrophosphokinase	ABF36968
Q04LP0	PrtA	91.64	96.83	0.0	serine protease	ABJ55268
Q99Z66	ptsI	89.95	99.83	0.0	Phosphoenolpyruvate-protein phosphotransferase	AAK34199
Q8DYL6	uvrB	89.59	99.85	0.0	Excinuclease ABC, B subunit	AE014259_2
Q8DPC2	potA	89.58	99.48	0.0	ABC transporter ATP-binding protein - spermidine/putrescine transport	AAL00050
P0C0B9	bglB	88.89	99.33	0.0	Putative phospho--glucosidase	AAK33487
D5AF41	IMPDH	86.81	99.80	0.0	Inosine-5'-monophosphate dehydrogenase	ADE32434
Q97S30	metF	86.01	98.96	0.0	Methionine biosynthesis enzymes	AAK74739
A4VYE0	gidA	85.26	98.75	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	ABP91129
P0A452	recA	84.67	98.71	0.0	DNA recombination and repair	AAL00560
B5TQV9	PGM	84.24	99.48	0.0	phosphoglucomutase	ACH87041
A4VWH9	Eno	84.14	99.31	0.0	Enolase	ABP90468
Q97RW0	SP_0676	83.44	93.50	0.0	Transcription facot	AAK74821
A4VWD1	mnmE	82.89	99.34	0.0	Central tRNA-modifying GTPase	ABP90420
A4VY17	pdh	82.20	99.41	0.0	Pyruvate dehydrogenase	ABP91006
Q93MY4	covR	86.44	99.58	1.67e-152	Responder protein	AAK71319
Q9S1K2	rr01	84.38	99.12	9.86e-145	Response regulator	CAB54566
R4Z8M6	ciaR	87.05	98.68	2.48e-144	Two component system response regulator	AIK75708
B3VA60	CovR	81.66	99.56	1.24e-137	serves to repress GAS virulence factor-encoding genes	ACE75886
U2W9V3	SpxA2	91.67	99.25	1.66e-88	Transcriptional regulator	ERL21461
U2UML5	SpxA1	82.84	99.26	1.34e-82	Transcriptional regulator	ERL19273
Q8DQV4	spr0479	85.42	97.96	3.98e-58	essential gene	AAK99283
Q8DP67	spr1327	92.94	98.84	1.61e-54	essential gene	AAL00131
Q8CZ89	spr0175	85.23	98.88	3.80e-52	pathogenitcity related	AAK98979
Q8DRE1	spr0177	83.15	87.25	1.46e-50	essential gene	AAK98981
Q04M32	ilvC (SPD_0406)	84.41	99.71	0.0	Ketol-acid reductoisomerase (NADP(+))	ABJ55451

BLASTP v2.8.1+ (Database: PHI-base v4.14, Nov. 2022 release)

Plasmid

PlasmidFinder

Plasmid	Identity	Query / Template	Accession no.
No hit found

PlasmidFinder 2.0.1 (Database: plasmidfinder_db, Jan. 2023 release)

Prophage

Phigaro

Scaffold	Begin	End	Transposable	Taxonomy	pVOGs
KQ971262.1	40460	48954	False	Siphoviridae	VOG3443, VOG7508, VOG4984, VOG4999, VOG4632, VOG8336, VOG8353

Phigaro v2.3.0 (Database: Aug. 2017 release)