Probiotic potential risk score
| ARGs (CARD) | VFs (VFDB) | PGs (PHI-base) | PPRS |
|---|---|---|---|
| 3 | 0 | 1 | 3.16 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| ARO accession | ARO name | Identity | Coverage | CARD name | Accession no. |
|---|---|---|---|---|---|
| ARO:3003905 | ANT(4')-Ib | 99.21 | 101.19 | ANT(4')-Ib | GQ900432.1 |
| ARO:3000194 | tet(W) | 97.29 | 92.49 | tet(W) | AJ222769.3 |
| ARO:3002835 | lnuA | 96.89 | 100.00 | lnuA | M14039.1 |
RGI v6.0.0 (Database: CARD Variants v4.0.0, Jul. 2022 release)
ResFinder
| Resistance gene | Identity | Coverage | Phenotype | Accession no. |
|---|---|---|---|---|
| ant(6)-Ia | 100.00 | 100.00 | Aminoglycoside resistance | KF864551 |
| aadD | 99.87 | 100.00 | Aminoglycoside resistance Alternate name; ant(4')-Ia and aadD2 | M19465 |
| ant(9)-Ia | 99.87 | 98.21 | Warning: gene is missing from Notes file. Please inform curator. | X02588 |
| tet(L) | 99.19 | 98.40 | Tetracycline resistance | AY081910 |
| tet(W) | 98.87 | 92.34 | Tetracycline resistance | FN396364 |
| lnu(A) | 98.56 | 100.00 | Lincosamide resistance | M14039 |
ResFinder v4.0 (Database: resfinder_db, May 2022 release)
AMRFinderPlus
| Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|
| aadD1 | aminoglycoside O-nucleotidyltransferase ANT(4')-Ia | EXACTX | 100.00 | 100.00 | WP_032488330.1 |
| aadE | aminoglycoside 6-adenylyltransferase AadE | EXACTX | 100.00 | 100.00 | WP_001255868.1 |
| tet(W) | tetracycline resistance ribosomal protection protein Tet(W) | BLASTX | 99.83 | 92.49 | WP_003672618.1 |
| lnu(A) | lincosamide nucleotidyltransferase Lnu(A) | BLASTX | 99.38 | 100.00 | WP_001829870.1 |
| tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 99.10 | 97.16 | WP_002294500.1 |
| ant(9)-Ia | aminoglycoside nucleotidyltransferase ANT(9)-Ia | INTERNAL_STOP | 98.84 | 99.62 | WP_000067268.1 |
AMRFinderPlus v3.10.42 (Database: Oct. 2022 release)
Virulence factors
Virulence Factor Database (VFDB)
| Gene ID | Gene name | Identity | Coverage | e-value | VF name | VF category | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
BLASTN v2.8.1+ (Database: VFDB, Jun. 2022 release)
VirulenceFinder
| Database | Virulence factor | Identity | Coverage | Protein function | Accession no. |
|---|---|---|---|---|---|
| No hit found | |||||
VirulenceFinder v2.0.3 (Database: virulencefinder_db, Dec. 2022 release)
Pathogenic genes
PHI-base
| Protein ID | Gene name | Identity | Coverage | e-value | Function | Gene ID |
|---|---|---|---|---|---|---|
| P0A355 | CspA | 84.61 | 97.01 | 3.80e-36 | Cold shock protein | CAA62903 |
BLASTP v2.8.1+ (Database: PHI-base v4.14, Nov. 2022 release)
Plasmid
PlasmidFinder
| Plasmid | Identity | Query / Template | Accession no. |
|---|---|---|---|
| rep30 | 97.43 | 777 / 777 | CP002845 |
| repUS67 | 95.00 | 1040 / 1039 | CP006012 |
PlasmidFinder 2.0.1 (Database: plasmidfinder_db, Jan. 2023 release)
Prophage
Phigaro
| Scaffold | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MIMJ01000103.1 | 22064 | 51024 | False | Siphoviridae | VOG0817, VOG0519, VOG4606, VOG0198, VOG4570, VOG10660, VOG1345, VOG4693, VOG8220, VOG4841, VOG4581, VOG1886, VOG4556, VOG4568, VOG0204, VOG0205, VOG0207, VOG4586, VOG0209, VOG0660, VOG4545, VOG0801, VOG10972, VOG4856, VOG1637, VOG0054 |
| MIMJ01000125.1 | 39607 | 51018 | False | Siphoviridae | VOG4900, VOG0650, VOG4632, VOG4606, VOG6414, VOG0205, VOG4841, VOG4581, VOG4544, VOG4556, VOG4568 |
Phigaro v2.3.0 (Database: Aug. 2017 release)