Probiotic potential risk score
| ARGs (CARD) | VFs (VFDB) | PGs (PHI-base) | PPRS |
|---|---|---|---|
| 4 | 0 | 1 | 4.12 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| ARO accession | ARO name | Identity | Coverage | CARD name | Accession no. |
|---|---|---|---|---|---|
| ARO:3000186 | tet(M) | 98.91 | 100.31 | tet(M) | AB039845.1 |
| ARO:3002704 | fexA | 97.68 | 100.00 | fexA | AJ549214.1 |
| ARO:3000194 | tet(W) | 97.02 | 100.31 | tet(W) | AJ222769.3 |
| ARO:3002835 | lnuA | 96.89 | 100.00 | lnuA | M14039.1 |
RGI v6.0.0 (Database: CARD Variants v4.0.0, Jul. 2022 release)
ResFinder
| Resistance gene | Identity | Coverage | Phenotype | Accession no. |
|---|---|---|---|---|
| tet(M) | 99.48 | 100.00 | Tetracycline resistance | X75073 |
| tet(W) | 99.06 | 99.64 | Tetracycline resistance | FN396364 |
| fexA | 98.39 | 100.00 | Phenicol resistance | AJ549214 |
| lnu(A) | 98.35 | 100.00 | Lincosamide resistance | M14039 |
ResFinder v4.0 (Database: resfinder_db, May 2022 release)
AMRFinderPlus
| Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|
| tet(W) | tetracycline resistance ribosomal protection protein Tet(W) | BLASTX | 99.84 | 99.84 | WP_000691721.1 |
| tet(M) | tetracycline resistance ribosomal protection protein Tet(M) | BLASTX | 99.53 | 100.00 | WP_000691727.1 |
| lnu(A) | lincosamide nucleotidyltransferase Lnu(A) | BLASTX | 99.38 | 100.00 | WP_001829870.1 |
| fexA | chloramphenicol/florfenicol efflux MFS transporter FexA | BLASTX | 97.68 | 100.00 | WP_015585966.1 |
AMRFinderPlus v3.10.42 (Database: Oct. 2022 release)
Virulence factors
Virulence Factor Database (VFDB)
| Gene ID | Gene name | Identity | Coverage | e-value | VF name | VF category | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
BLASTN v2.8.1+ (Database: VFDB, Jun. 2022 release)
VirulenceFinder
| Database | Virulence factor | Identity | Coverage | Protein function | Accession no. |
|---|---|---|---|---|---|
| No hit found | |||||
VirulenceFinder v2.0.3 (Database: virulencefinder_db, Dec. 2022 release)
Pathogenic genes
PHI-base
| Protein ID | Gene name | Identity | Coverage | e-value | Function | Gene ID |
|---|---|---|---|---|---|---|
| P0A355 | CspA | 84.61 | 97.01 | 3.46e-36 | Cold shock protein | CAA62903 |
BLASTP v2.8.1+ (Database: PHI-base v4.14, Nov. 2022 release)
Plasmid
PlasmidFinder
| Plasmid | Identity | Query / Template | Accession no. |
|---|---|---|---|
| No hit found | |||
PlasmidFinder 2.0.1 (Database: plasmidfinder_db, Jan. 2023 release)
Prophage
Phigaro
| Scaffold | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MIMN01000016.1 | 2229 | 11436 | True | Unknown | VOG0641, VOG9502, VOG9409, VOG0221, VOG0221, VOG4548 |
| MIMN01000033.1 | 54329 | 88350 | False | Siphoviridae | VOG4824, VOG0054, VOG1637, VOG3398, VOG5372, VOG6163, VOG4633, VOG4545, VOG0800, VOG0725, VOG0799, VOG0724, VOG5660, VOG0723, VOG4713, VOG1319, VOG4555, VOG7533, VOG4564, VOG0720, VOG10227, VOG0796, VOG0198, VOG3307, VOG3307, VOG0327, VOG6165, VOG5278, VOG4609, VOG4632, VOG6545, VOG0322, VOG4548, VOG0321, VOG5720, VOG1638, VOG2228, VOG10195, VOG9667 |
Phigaro v2.3.0 (Database: Aug. 2017 release)