Probiotic potential risk score

ARGs (CARD)	VFs (VFDB)	PGs (PHI-base)	PPRS
0	24	28	36.88

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

ARO accession	ARO name	Identity	Coverage	CARD name	Accession no.
No hit found

RGI v6.0.0 (Database: CARD Variants v4.0.0, Jul. 2022 release)

ResFinder

Resistance gene	Identity	Coverage	Phenotype	Accession no.
No hit found

ResFinder v4.0 (Database: resfinder_db, May 2022 release)

AMRFinderPlus

Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v3.10.42 (Database: Oct. 2022 release)

Virulence factors

Virulence Factor Database (VFDB)

Gene ID	Gene name	Identity	Coverage	e-value	VF name	VF category	Accession no.
VFG005322	cbpD	100.00	100.00	0.0	Autolysin	Exoenzyme	WP_011226733
VFG006041	rfbA	100.00	100.00	0.0	Capsule	Immune modulation	WP_011681286
VFG006032	STER_RS06030	100.00	100.00	0.0	Capsule	Immune modulation	WP_011226149
VFG005556	tig/ropA	100.00	100.00	0.0	Trigger factor	Stress survival	WP_011680653
VFG006084	STU_RS16245	99.88	100.00	0.0	Capsule	Immune modulation	WP_002948020
VFG006086	STU_RS16250	99.79	100.00	0.0	Capsule	Immune modulation	WP_011226328
VFG006088	STU_RS16255	99.74	100.00	0.0	Capsule	Immune modulation	WP_041828290
VFG006022	rfbB	99.71	100.19	0.0	Capsule	Immune modulation	WP_002947383
VFG005191	fbp54	99.70	100.00	0.0	FBPs	Adherence	WP_011681101
VFG006081	rfbD	99.65	100.00	0.0	Capsule	Immune modulation	WP_014621820
VFG006082	STU_RS16240	99.58	100.00	0.0	Capsule	Immune modulation	WP_011226326
VFG006074	STU_RS16230	99.50	100.00	0.0	Capsule	Immune modulation	WP_011226325
VFG006090	STU_RS16260	99.50	100.00	0.0	Capsule	Immune modulation	WP_011226330
VFG006078	STU_RS16235	99.43	100.00	0.0	Capsule	Immune modulation	WP_002948022
VFG005381	srtA	99.34	100.00	0.0	Sortase A	Exoenzyme	-
VFG005844	STER_RS05250	98.52	100.00	0.0	Capsule	Immune modulation	WP_011681165
VFG006053	STU_RS14645	98.36	100.00	0.0	Capsule	Immune modulation	WP_011226048
VFG006011	STER_RS05320	97.54	100.00	0.0	Capsule	Immune modulation	WP_011681177
VFG006091	STU_RS16265	97.48	99.76	0.0	Capsule	Immune modulation	WP_011226331
VFG006040	STU_RS14635	97.30	100.00	0.0	Capsule	Immune modulation	WP_011226046
VFG006009	STR_RS05255	95.40	99.86	0.0	Capsule	Immune modulation	WP_011227247
VFG006031	STU_RS14630	95.39	100.00	0.0	Capsule	Immune modulation	WP_011226045
VFG019077	plr/gapA	93.48	100.00	0.0	Streptococcal plasmin receptor/GAPDH	Adherence	WP_000260685
VFG005580	eno	90.20	99.92	0.0	Streptococcal enolase	Exoenzyme	WP_000022813

BLASTN v2.8.1+ (Database: VFDB, Jun. 2022 release)

VirulenceFinder

Database	Virulence factor	Identity	Coverage	Protein function	Accession no.
No hit found

VirulenceFinder v2.0.3 (Database: virulencefinder_db, Dec. 2022 release)

Pathogenic genes

PHI-base

Protein ID	Gene name	Identity	Coverage	e-value	Function	Gene ID
Q1JLQ2	MntE	94.41	99.44	0.0	Manganese Homeostasis	ABF32167
P67331	spr0084	92.57	98.18	0.0	pathogenitcity related	AAK98888
Q97S31	metE	91.99	99.87	0.0	Methionine biosynthesis enzymes	AAK74738
Q1J943	prsA2	90.62	98.46	0.0	Ribose-phosphate pyrophosphokinase	ABF36968
Q8DPC2	potA	89.58	99.48	0.0	ABC transporter ATP-binding protein - spermidine/putrescine transport	AAL00050
Q8DYL6	uvrB	88.84	99.85	0.0	Excinuclease ABC, B subunit	AE014259_2
P0C0B9	bglB	88.55	99.33	0.0	Putative phospho--glucosidase	AAK33487
Q99Z66	ptsI	87.87	99.83	0.0	Phosphoenolpyruvate-protein phosphotransferase	AAK34199
D5AF41	IMPDH	87.42	99.80	0.0	Inosine-5'-monophosphate dehydrogenase	ADE32434
A4VYE0	gidA	86.05	98.75	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	ABP91129
Q97S30	metF	86.01	98.96	0.0	Methionine biosynthesis enzymes	AAK74739
A4VWH9	Eno	85.98	99.54	0.0	Enolase	ABP90468
P0A452	recA	84.72	98.71	0.0	DNA recombination and repair	AAL00560
B5TQV9	PGM	83.71	99.48	0.0	phosphoglucomutase	ACH87041
Q97RW0	SP_0676	83.06	93.19	0.0	Transcription facot	AAK74821
A4VY17	pdh	82.79	99.41	0.0	Pyruvate dehydrogenase	ABP91006
A4VWD1	mnmE	81.80	99.34	0.0	Central tRNA-modifying GTPase	ABP90420
Q1JJJ3	PepO	80.19	99.84	0.0	Endopeptidase	ABF32980
Q93MY4	covR	85.53	99.58	2.72e-151	Responder protein	AAK71319
Q9S1K2	rr01	83.04	99.12	5.26e-142	Response regulator	CAB54566
B3VA60	CovR	80.79	99.56	4.02e-136	serves to repress GAS virulence factor-encoding genes	ACE75886
U2W9V3	SpxA2	90.91	99.25	3.08e-88	Transcriptional regulator	ERL21461
U2UML5	SpxA1	80.60	99.26	4.93e-79	Transcriptional regulator	ERL19273
Q8DQV4	spr0479	86.46	97.96	1.04e-58	essential gene	AAK99283
Q8DP67	spr1327	94.12	98.84	3.42e-54	essential gene	AAL00131
Q8DRE1	spr0177	84.27	87.25	2.13e-51	essential gene	AAK98981
Q8CZ89	spr0175	84.09	98.88	3.92e-51	pathogenitcity related	AAK98979
Q04M32	ilvC (SPD_0406)	96.47	99.71	0.0	Ketol-acid reductoisomerase (NADP(+))	ABJ55451

BLASTP v2.8.1+ (Database: PHI-base v4.14, Nov. 2022 release)

Plasmid

PlasmidFinder

Plasmid	Identity	Query / Template	Accession no.
No hit found

PlasmidFinder 2.0.1 (Database: plasmidfinder_db, Jan. 2023 release)

Prophage

Phigaro

Scaffold	Begin	End	Transposable	Taxonomy	pVOGs
No hit found

Phigaro v2.3.0 (Database: Aug. 2017 release)