Probiotic potential risk score

ARGs (CARD)	VFs (VFDB)	PGs (PHI-base)	PPRS
1	2	7	7.35

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

ARO accession	ARO name	Identity	Coverage	CARD name	Accession no.
ARO:3002814	clbA	99.71	104.01	clbA	CP006845.1

RGI v6.0.0 (Database: CARD Variants v4.0.0, Jul. 2022 release)

ResFinder

Resistance gene	Identity	Coverage	Phenotype	Accession no.
cfr(B)	88.71	98.67	Warning: gene is missing from Notes file. Please inform curator.	KR610408
tet(L)	86.86	99.93	Tetracycline resistance	X08034

ResFinder v4.0 (Database: resfinder_db, May 2022 release)

AMRFinderPlus

Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
clbA	23S rRNA (adenine(2503)-C(8))-methyltransferase ClbA	BLASTX	99.71	98.85	WP_012116915.1
tet(L)	tetracycline efflux MFS transporter Tet(L)	PARTIALX	89.47	74.67	WP_003242953.1

AMRFinderPlus v3.10.42 (Database: Oct. 2022 release)

Virulence factors

Virulence Factor Database (VFDB)

Gene ID	Gene name	Identity	Coverage	e-value	VF name	VF category	Accession no.
VFG016299	capB	82.06	99.58	0.0	Capsule	Immune modulation	NP_391471
VFG016295	capC	83.37	99.78	5.67e-117	Capsule	Immune modulation	NP_391470

BLASTN v2.8.1+ (Database: VFDB, Jun. 2022 release)

VirulenceFinder

Database	Virulence factor	Identity	Coverage	Protein function	Accession no.
No hit found

VirulenceFinder v2.0.3 (Database: virulencefinder_db, Dec. 2022 release)

Pathogenic genes

PHI-base

Protein ID	Gene name	Identity	Coverage	e-value	Function	Gene ID
W8TRT9	tufA	86.80	99.24	0.0	Elongation factor Tu	AHM69299
B3GXB6	pdxS	84.83	97.97	0.0	Encodes the putative pyridoxal 5�-phosphate synthase subunit PdxS	ACE61268
B7HLG1	CodY	82.19	95.00	9.96e-143	Nutrient-responsive regulator	ACJ80679
Q9RGX0	spxA1	83.21	99.24	3.54e-82	Redox-responsive transcription factor	AAF21893
P0A355	CspA	90.91	98.51	7.75e-40	Cold shock protein	CAA62903
Q92AD0	CspD	80.00	97.01	1.09e-35	Cold shock protein	CAC99957
P0A357	CspB	84.61	97.01	9.47e-35	Cold shock protein	CAD00094

BLASTP v2.8.1+ (Database: PHI-base v4.14, Nov. 2022 release)

Plasmid

PlasmidFinder

Plasmid	Identity	Query / Template	Accession no.
No hit found

PlasmidFinder 2.0.1 (Database: plasmidfinder_db, Jan. 2023 release)

Prophage

Phigaro

Scaffold	Begin	End	Transposable	Taxonomy	pVOGs
CP035899.1	2058544	2079182	False	Siphoviridae	VOG0796, VOG10439, VOG0720, VOG0720, VOG4564, VOG4555, VOG4553, VOG1125, VOG0815, VOG4623, VOG0816, VOG0724, VOG0799, VOG2621, VOG0725, VOG1323, VOG4545, VOG4605, VOG4599
CP035899.1	2356168	2380633	False	Myoviridae	VOG8642, VOG0796, VOG0720, VOG1183, VOG0045, VOG4555, VOG4572, VOG1643, VOG4564, VOG4623, VOG1353, VOG1881, VOG1883, VOG1352, VOG4699, VOG9745, VOG6163, VOG1956, VOG1433, VOG1348, VOG0573, VOG4550, VOG4691, VOG4845, VOG7718, VOG3390, VOG4705, VOG0753, VOG0136, VOG10230
CP035899.1	2434437	2463149	False	Siphoviridae	VOG3547, VOG11060, VOG9600, VOG0025, VOG1884, VOG10961, VOG2981, VOG5447, VOG0376, VOG2545, VOG0531, VOG4841, VOG0713, VOG4581, VOG1886, VOG3607, VOG4556, VOG4568, VOG4553, VOG7006, VOG0204, VOG1266, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG0801, VOG4772, VOG4619, VOG4660, VOG4660, VOG4612, VOG3390
CP035899.1	3847550	3875152	False	Myoviridae	VOG0753, VOG4705, VOG3390, VOG4894, VOG4612, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG7947, VOG3892, VOG3891, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667, VOG4602

Phigaro v2.3.0 (Database: Aug. 2017 release)