Probiotic potential risk score
| ARGs (CARD) | VFs (VFDB) | PGs (PHI-base) | PPRS |
|---|---|---|---|
| 3 | 0 | 2 | 3.61 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| ARO accession | ARO name | Identity | Coverage | CARD name | Accession no. |
|---|---|---|---|---|---|
| ARO:3000179 | tet(L) | 99.13 | 100.00 | tet(L) | M11036.1 |
| ARO:3000186 | tet(M) | 98.13 | 100.31 | tet(M) | AB039845.1 |
| ARO:3002835 | lnuA | 97.52 | 100.00 | lnuA | M14039.1 |
RGI v6.0.0 (Database: CARD Variants v4.0.0, Jul. 2022 release)
ResFinder
| Resistance gene | Identity | Coverage | Phenotype | Accession no. |
|---|---|---|---|---|
| tet(L) | 99.78 | 100.00 | Tetracycline resistance | M29725 |
| tet(M) | 99.12 | 100.00 | Tetracycline resistance | X75073 |
| lnu(A) | 98.77 | 100.00 | Lincosamide resistance | M14039 |
ResFinder v4.0 (Database: resfinder_db, May 2022 release)
AMRFinderPlus
| Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|
| lnu(A) | lincosamide nucleotidyltransferase Lnu(A) | EXACTX | 100.00 | 100.00 | WP_001829870.1 |
| tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 99.34 | 100.00 | WP_001574277.1 |
| tet(M) | tetracycline resistance ribosomal protection protein Tet(M) | BLASTX | 98.91 | 100.00 | WP_000691736.1 |
AMRFinderPlus v3.10.42 (Database: Oct. 2022 release)
Virulence factors
Virulence Factor Database (VFDB)
| Gene ID | Gene name | Identity | Coverage | e-value | VF name | VF category | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
BLASTN v2.8.1+ (Database: VFDB, Jun. 2022 release)
VirulenceFinder
| Database | Virulence factor | Identity | Coverage | Protein function | Accession no. |
|---|---|---|---|---|---|
| No hit found | |||||
VirulenceFinder v2.0.3 (Database: virulencefinder_db, Dec. 2022 release)
Pathogenic genes
PHI-base
| Protein ID | Gene name | Identity | Coverage | e-value | Function | Gene ID |
|---|---|---|---|---|---|---|
| S4E4Q5 | WalR | 85.59 | 100.00 | 3.14e-148 | Transcriptional regulatory protein | EPH95667 |
| Q82ZX2 | CspR | 81.82 | 98.51 | 2.70e-36 | Cold shock protein | AAO82613 |
BLASTP v2.8.1+ (Database: PHI-base v4.14, Nov. 2022 release)
Plasmid
PlasmidFinder
| Plasmid | Identity | Query / Template | Accession no. |
|---|---|---|---|
| No hit found | |||
PlasmidFinder 2.0.1 (Database: plasmidfinder_db, Jan. 2023 release)
Prophage
Phigaro
| Scaffold | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| JAEHNU010000007.1 | 184606 | 207148 | False | Siphoviridae | VOG5278, VOG0195, VOG0198, VOG1315, VOG0796, VOG10227, VOG0720, VOG4564, VOG0122, VOG10914, VOG4572, VOG4713, VOG0723, VOG5660, VOG0724, VOG0799, VOG0725, VOG0800, VOG4545, VOG4633, VOG5904, VOG5008, VOG0641 |
| JAEHNU010000008.1 | 128920 | 165283 | False | Siphoviridae | VOG9667, VOG3947, VOG0321, VOG4548, VOG0322, VOG6545, VOG4632, VOG4609, VOG0327, VOG5998, VOG5562, VOG0275, VOG5278, VOG0195, VOG1724, VOG4918, VOG0638, VOG7894, VOG4841, VOG4581, VOG1886, VOG4556, VOG4573, VOG4553, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG5852, VOG4545, VOG0801, VOG3462, VOG4190, VOG4856, VOG5008 |
Phigaro v2.3.0 (Database: Aug. 2017 release)