Probiotic potential risk score

ARGs (CARD)	VFs (VFDB)	PGs (PHI-base)	PPRS
0	0	2	2.00

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

ARO accession	ARO name	Identity	Coverage	CARD name	Accession no.
No hit found

RGI v6.0.0 (Database: CARD Variants v4.0.0, Jul. 2022 release)

ResFinder

Resistance gene	Identity	Coverage	Phenotype	Accession no.
blaTEM-168	99.32	85.37	Beta-lactam resistance	FJ919776
blaTEM-1A	99.32	85.37	Beta-lactam resistance Alternate name; RblaTEM-1	HM749966
blaTEM-82	99.32	85.37	Beta-lactam resistance	AF427128

ResFinder v4.0 (Database: resfinder_db, May 2022 release)

AMRFinderPlus

Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
blaTEM	TEM family class A beta-lactamase	PARTIAL_CONTIG_ENDX	98.77	85.31	WP_013279314.1

AMRFinderPlus v3.10.42 (Database: Oct. 2022 release)

Virulence factors

Virulence Factor Database (VFDB)

Gene ID	Gene name	Identity	Coverage	e-value	VF name	VF category	Accession no.
No hit found

BLASTN v2.8.1+ (Database: VFDB, Jun. 2022 release)

VirulenceFinder

Database	Virulence factor	Identity	Coverage	Protein function	Accession no.
No hit found

VirulenceFinder v2.0.3 (Database: virulencefinder_db, Dec. 2022 release)

Pathogenic genes

PHI-base

Protein ID	Gene name	Identity	Coverage	e-value	Function	Gene ID
S4E4Q5	WalR	84.68	99.57	1.82e-144	Transcriptional regulatory protein	EPH95667
Q82ZX2	CspR	87.88	98.51	1.64e-40	Cold shock protein	AAO82613

BLASTP v2.8.1+ (Database: PHI-base v4.14, Nov. 2022 release)

Plasmid

PlasmidFinder

Plasmid	Identity	Query / Template	Accession no.
repUS75	100.00	1059 / 1059	CP002393
repUS64	92.59	958 / 954	JN601038

PlasmidFinder 2.0.1 (Database: plasmidfinder_db, Jan. 2023 release)

Prophage

Phigaro

Scaffold	Begin	End	Transposable	Taxonomy	pVOGs
JAFEHI010000010.1	97973	104303	False	Unknown	VOG1309, VOG0186, VOG4566, VOG0189, VOG6623, VOG4693, VOG4813, VOG9759
JAFEHI010000022.1	1813	22431	False	Siphoviridae	VOG4649, VOG0602, VOG7017, VOG0861, VOG4856, VOG4619, VOG0801, VOG1323, VOG0725, VOG0799, VOG1321, VOG9583, VOG1320, VOG4713, VOG1322, VOG0694, VOG4555, VOG4564, VOG0720, VOG10227, VOG3699
JAFEHI010000023.1	1211	20999	False	Siphoviridae	VOG0054, VOG1637, VOG4705, VOG4599, VOG4605, VOG6163, VOG0660, VOG0209, VOG4586, VOG0207, VOG0205, VOG9975, VOG4553, VOG4573, VOG4556, VOG1886, VOG4581, VOG4841

Phigaro v2.3.0 (Database: Aug. 2017 release)