Probiotic potential risk score

ARGs (CARD)	VFs (VFDB)	PGs (PHI-base)	PPRS
8	10	9	15.65

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

ARO accession	ARO name	Identity	Coverage	CARD name	Accession no.
ARO:3003324	Bacillus subtilis mprF	99.88	100.00	Bsub_mprF	AL009126.3
ARO:3002813	lmrB	99.79	100.42	lmrB	JYFL01000006.1
ARO:3003007	bmr	99.49	100.00	bmr	M33768.1
ARO:3003064	ykkD	98.10	100.00	ykkD	AL009126.1
ARO:3003006	blt	98.00	100.00	blt	L32599.1
ARO:3004476	vmlR	97.97	98.90	vmlR	NC_000964.3
ARO:3003063	ykkC	97.32	100.00	ykkC	AL009126.1
ARO:3002627	aadK	96.13	101.41	aadK	AL009126.1

RGI v6.0.0 (Database: CARD Variants v4.0.0, Jul. 2022 release)

ResFinder

Resistance gene	Identity	Coverage	Phenotype	Accession no.
aadK	97.78	100.00	Aminoglycoside resistance	M26879
mph(K)	93.49	99.89	Warning: gene is missing from Notes file. Please inform curator.	NC_000964

ResFinder v4.0 (Database: resfinder_db, May 2022 release)

AMRFinderPlus

Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
vmlR	ABC-F type ribosomal protection protein VmlR	BLASTX	97.97	98.90	WP_003234144.1
aadK	aminoglycoside 6-adenylyltransferase AadK	BLASTX	96.13	100.00	WP_003229862.1
mphK	macrolide 2'-phosphotransferase MphK	INTERNAL_STOP	93.14	100.00	WP_003246254.1
satA	streptothricin N-acetyltransferase SatA	BLASTX	91.33	100.00	WP_003242546.1
rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.06	100.00	WP_087347987.1

AMRFinderPlus v3.10.42 (Database: Oct. 2022 release)

Virulence factors

Virulence Factor Database (VFDB)

Gene ID	Gene name	Identity	Coverage	e-value	VF name	VF category	Accession no.
VFG049999	dhbA	99.75	100.00	0.0	Bacillibactin	Nutritional/Metabolic factor	NP_391080
VFG016299	capB	99.58	100.00	0.0	Capsule	Immune modulation	NP_391471
VFG016234	hlyIII	99.38	100.00	0.0	Hemolysin III	Exotoxin	NP_390062
VFG050032	dhbB	99.36	100.00	0.0	Bacillibactin	Nutritional/Metabolic factor	NP_391077
VFG016295	capC	98.89	100.00	0.0	Capsule	Immune modulation	NP_391470
VFG016303	dep/capD	98.75	100.00	0.0	Capsule	Immune modulation	NP_389723
VFG050010	dhbC	98.75	100.00	0.0	Bacillibactin	Nutritional/Metabolic factor	NP_391079
VFG016291	capA	98.51	100.00	0.0	Capsule	Immune modulation	NP_391469
VFG050043	dhbF	98.49	100.00	0.0	Bacillibactin	Nutritional/Metabolic factor	NP_391076
VFG050021	dhbE	98.27	100.00	0.0	Bacillibactin	Nutritional/Metabolic factor	NP_391078

BLASTN v2.8.1+ (Database: VFDB, Jun. 2022 release)

VirulenceFinder

Database	Virulence factor	Identity	Coverage	Protein function	Accession no.
No hit found

VirulenceFinder v2.0.3 (Database: virulencefinder_db, Dec. 2022 release)

Pathogenic genes

PHI-base

Protein ID	Gene name	Identity	Coverage	e-value	Function	Gene ID
W8TRT9	tufA	85.03	99.24	0.0	Elongation factor Tu	AHM69299
B3GXB6	pdxS	83.79	97.97	0.0	Encodes the putative pyridoxal 5�-phosphate synthase subunit PdxS	ACE61268
B7HLG1	CodY	81.08	99.62	5.53e-148	Nutrient-responsive regulator	ACJ80679
P63786	ClpP	81.05	96.94	1.57e-114	Part of proteolytic complex	BAB94595
Q9RGX0	spxA1	83.97	99.24	8.94e-83	Redox-responsive transcription factor	AAF21893
Q724L7	SpoVG	82.72	78.64	2.30e-47	RNA-Binding Protein	AAT02994
P0A355	CspA	90.91	98.51	7.59e-40	Cold shock protein	CAA62903
Q92AD0	CspD	81.54	97.01	1.97e-36	Cold shock protein	CAC99957
P0A357	CspB	83.08	97.01	2.90e-34	Cold shock protein	CAD00094

BLASTP v2.8.1+ (Database: PHI-base v4.14, Nov. 2022 release)

Plasmid

PlasmidFinder

Plasmid	Identity	Query / Template	Accession no.
No hit found

PlasmidFinder 2.0.1 (Database: plasmidfinder_db, Jan. 2023 release)

Prophage

Phigaro

Scaffold	Begin	End	Transposable	Taxonomy	pVOGs
JAHHXT010000002.1	209306	236122	False	Myoviridae	VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4843, VOG0189, VOG3490

Phigaro v2.3.0 (Database: Aug. 2017 release)