Probiotic potential risk score

ARGs (CARD)	VFs (VFDB)	PGs (PHI-base)	PPRS
1	23	28	36.25

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

ARO accession	ARO name	Identity	Coverage	CARD name	Accession no.
ARO:3000192	tet(S)	99.84	100.78	tet(S)	L09756.1

RGI v6.0.0 (Database: CARD Variants v4.0.0, Jul. 2022 release)

ResFinder

Resistance gene	Identity	Coverage	Phenotype	Accession no.
tet(S)	100.00	100.00	Tetracycline resistance	L09756

ResFinder v4.0 (Database: resfinder_db, May 2022 release)

AMRFinderPlus

Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
tet(S)	tetracycline resistance ribosomal protection protein Tet(S)	EXACTX	100.00	100.00	WP_000691722.1

AMRFinderPlus v3.10.42 (Database: Oct. 2022 release)

Virulence factors

Virulence Factor Database (VFDB)

Gene ID	Gene name	Identity	Coverage	e-value	VF name	VF category	Accession no.
VFG006022	rfbB	100.00	100.00	0.0	Capsule	Immune modulation	WP_002947383
VFG005388	cshA	99.76	71.59	0.0	Cell surface hydrophobicity proteins	Adherence	-
VFG006084	STU_RS16245	99.75	100.00	0.0	Capsule	Immune modulation	WP_002948020
VFG005191	fbp54	99.58	100.00	0.0	FBPs	Adherence	WP_011681101
VFG005556	tig/ropA	99.53	100.00	0.0	Trigger factor	Stress survival	WP_011680653
VFG006086	STU_RS16250	99.27	100.00	0.0	Capsule	Immune modulation	WP_011226328
VFG006011	STER_RS05320	99.25	100.00	0.0	Capsule	Immune modulation	WP_011681177
VFG006082	STU_RS16240	99.25	100.00	0.0	Capsule	Immune modulation	WP_011226326
VFG006088	STU_RS16255	99.22	100.00	0.0	Capsule	Immune modulation	WP_041828290
VFG006074	STU_RS16230	99.22	99.89	0.0	Capsule	Immune modulation	WP_011226325
VFG006070	rfbA	99.19	100.00	0.0	Capsule	Immune modulation	WP_011226150
VFG006046	STR_RS05880	99.16	100.00	0.0	Capsule	Immune modulation	WP_011226149
VFG006078	STU_RS16235	99.15	100.00	0.0	Capsule	Immune modulation	WP_002948022
VFG006081	rfbD	99.06	100.00	0.0	Capsule	Immune modulation	WP_014621820
VFG005381	srtA	98.69	100.00	0.0	Sortase A	Exoenzyme	-
VFG005325	cbpD	98.57	100.00	0.0	Autolysin	Exoenzyme	WP_011680595
VFG006000	STER_RS05315	98.50	100.00	0.0	Capsule	Immune modulation	WP_011681176
VFG005976	STER_RS05305	96.76	100.00	0.0	Capsule	Immune modulation	WP_011681174
VFG006047	STU_RS14640	95.96	100.00	0.0	Capsule	Immune modulation	WP_011226047
VFG005964	STER_RS05300	95.39	99.85	0.0	Capsule	Immune modulation	WP_011681173
VFG019077	plr/gapA	93.97	100.20	0.0	Streptococcal plasmin receptor/GAPDH	Adherence	WP_000260685
VFG005580	eno	90.20	99.92	0.0	Streptococcal enolase	Exoenzyme	WP_000022813
VFG006004	SPD_RS01775	82.73	96.20	2.68e-148	Capsule	Immune modulation	WP_001819836

BLASTN v2.8.1+ (Database: VFDB, Jun. 2022 release)

VirulenceFinder

Database	Virulence factor	Identity	Coverage	Protein function	Accession no.
No hit found

VirulenceFinder v2.0.3 (Database: virulencefinder_db, Dec. 2022 release)

Pathogenic genes

PHI-base

Protein ID	Gene name	Identity	Coverage	e-value	Function	Gene ID
Q1JLQ2	MntE	94.97	99.44	0.0	Manganese Homeostasis	ABF32167
P67331	spr0084	92.88	98.18	0.0	pathogenitcity related	AAK98888
Q97S31	metE	91.86	99.87	0.0	Methionine biosynthesis enzymes	AAK74738
Q1J943	prsA2	90.62	98.46	0.0	Ribose-phosphate pyrophosphokinase	ABF36968
Q8DPC2	potA	89.58	99.48	0.0	ABC transporter ATP-binding protein - spermidine/putrescine transport	AAL00050
Q8DYL6	uvrB	89.14	99.85	0.0	Excinuclease ABC, B subunit	AE014259_2
P0C0B9	bglB	88.89	99.33	0.0	Putative phospho--glucosidase	AAK33487
Q99Z66	ptsI	87.87	99.83	0.0	Phosphoenolpyruvate-protein phosphotransferase	AAK34199
D5AF41	IMPDH	87.63	99.80	0.0	Inosine-5'-monophosphate dehydrogenase	ADE32434
A4VWH9	Eno	85.98	99.54	0.0	Enolase	ABP90468
A4VYE0	gidA	85.74	98.75	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	ABP91129
Q97S30	metF	85.66	98.96	0.0	Methionine biosynthesis enzymes	AAK74739
P0A452	recA	84.94	98.46	0.0	DNA recombination and repair	AAL00560
B5TQV9	PGM	83.71	99.48	0.0	phosphoglucomutase	ACH87041
A4VY17	pdh	83.09	99.41	0.0	Pyruvate dehydrogenase	ABP91006
Q97RW0	SP_0676	82.45	93.50	0.0	Transcription facot	AAK74821
A4VWD1	mnmE	81.80	99.34	0.0	Central tRNA-modifying GTPase	ABP90420
Q93MY4	covR	85.53	99.58	3.00e-151	Responder protein	AAK71319
Q9S1K2	rr01	84.38	99.12	1.61e-144	Response regulator	CAB54566
P0A4H7	CiaR	87.44	99.11	3.31e-143	Two-component response regulator	AAK74935
B3VA60	CovR	81.22	99.56	1.14e-136	serves to repress GAS virulence factor-encoding genes	ACE75886
U2W9V3	SpxA2	90.91	99.25	3.40e-88	Transcriptional regulator	ERL21461
U2UML5	SpxA1	80.60	99.26	6.70e-79	Transcriptional regulator	ERL19273
Q8DQV4	spr0479	84.38	97.96	5.16e-58	essential gene	AAK99283
Q8DP67	spr1327	95.29	98.84	4.20e-55	essential gene	AAL00131
Q8DRE1	spr0177	84.27	87.25	2.35e-51	essential gene	AAK98981
Q8CZ89	spr0175	84.09	98.88	4.33e-51	pathogenitcity related	AAK98979
Q04M32	ilvC (SPD_0406)	96.47	99.71	0.0	Ketol-acid reductoisomerase (NADP(+))	ABJ55451

BLASTP v2.8.1+ (Database: PHI-base v4.14, Nov. 2022 release)

Plasmid

PlasmidFinder

Plasmid	Identity	Query / Template	Accession no.
No hit found

PlasmidFinder 2.0.1 (Database: plasmidfinder_db, Jan. 2023 release)

Prophage

Phigaro

Scaffold	Begin	End	Transposable	Taxonomy	pVOGs
No hit found

Phigaro v2.3.0 (Database: Aug. 2017 release)