Probiotic potential risk score
| ARGs (CARD) | VFs (VFDB) | PGs (PHI-base) | PPRS |
|---|---|---|---|
| 5 | 1 | 4 | 6.48 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| ARO accession | ARO name | Identity | Coverage | CARD name | Accession no. |
|---|---|---|---|---|---|
| ARO:3002816 | clbC | 100.00 | 100.00 | clbC | AP006627.1 |
| ARO:3004749 | BCL-1 | 100.00 | 98.05 | BCL-1 | EF540343.1 |
| ARO:3002623 | ANT(4')-Ia | 99.22 | 100.00 | ANT(4')-Ia | EF540343.1 |
| ARO:3000600 | Erm(34) | 96.80 | 100.00 | Erm(34) | AY234334.1 |
| ARO:3004452 | Alkalihalobacillus clausii chloramphenicol acetyltransferase | 88.68 | 94.30 | Acla_ACT_CHL | AY238971.1 |
RGI v6.0.0 (Database: CARD Variants v4.0.0, Jul. 2022 release)
ResFinder
| Resistance gene | Identity | Coverage | Phenotype | Accession no. |
|---|---|---|---|---|
| ant(4')-Ib | 99.09 | 100.00 | Aminoglycoside resistance | AJ506108 |
| cat | 97.52 | 98.40 | Phenicol resistance | AY238971 |
| erm(34) | 95.47 | 99.17 | Macrolide resistance | AY234334 |
ResFinder v4.0 (Database: resfinder_db, May 2022 release)
AMRFinderPlus
| Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|
| blaBCL-1 | class A beta-lactamase BCL-1 | ALLELEX | 100.00 | 100.00 | WP_065825670.1 |
| catA | type A-10 chloramphenicol O-acetyltransferase | EXACTX | 100.00 | 100.00 | WP_035203840.1 |
| clbB | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbB | EXACTX | 100.00 | 100.00 | WP_011245929.1 |
| aadD2 | aminoglycoside O-nucleotidyltransferase ANT(4')-Ib | BLASTX | 99.22 | 100.00 | WP_035202393.1 |
| erm(34) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(34) | BLASTX | 96.80 | 100.00 | WP_063844504.1 |
AMRFinderPlus v3.10.42 (Database: Oct. 2022 release)
Virulence factors
Virulence Factor Database (VFDB)
| Gene ID | Gene name | Identity | Coverage | e-value | VF name | VF category | Accession no. |
|---|---|---|---|---|---|---|---|
| VFG016238 | hlyIII | 99.69 | 100.00 | 0.0 | Hemolysin III | Exotoxin | WP_011247375 |
BLASTN v2.8.1+ (Database: VFDB, Jun. 2022 release)
VirulenceFinder
| Database | Virulence factor | Identity | Coverage | Protein function | Accession no. |
|---|---|---|---|---|---|
| No hit found | |||||
VirulenceFinder v2.0.3 (Database: virulencefinder_db, Dec. 2022 release)
Pathogenic genes
PHI-base
| Protein ID | Gene name | Identity | Coverage | e-value | Function | Gene ID |
|---|---|---|---|---|---|---|
| W8TRT9 | tufA | 87.31 | 99.24 | 0.0 | Elongation factor Tu | AHM69299 |
| Q724L7 | SpoVG | 81.48 | 78.64 | 7.00e-45 | RNA-Binding Protein | AAT02994 |
| P0A355 | CspA | 86.15 | 97.01 | 1.46e-37 | Cold shock protein | CAA62903 |
| Q2FH36 | msaB | 81.25 | 95.52 | 2.52e-33 | Cold shock protein | ABD22065 |
BLASTP v2.8.1+ (Database: PHI-base v4.14, Nov. 2022 release)
Plasmid
PlasmidFinder
| Plasmid | Identity | Query / Template | Accession no. |
|---|---|---|---|
| No hit found | |||
PlasmidFinder 2.0.1 (Database: plasmidfinder_db, Jan. 2023 release)
Prophage
Phigaro
| Scaffold | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| JAIEWK010000007.1 | 78422 | 110434 | False | Myoviridae | VOG8979, VOG0992, VOG0602, VOG0753, VOG4705, VOG3390, VOG5384, VOG4862, VOG4691, VOG3894, VOG4885, VOG4865, VOG4910, VOG3892, VOG3891, VOG3890, VOG10055, VOG5660, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG7407, VOG0796, VOG5447 |
| JAIEWK010000009.1 | 55411 | 92917 | False | Myoviridae / Siphoviridae | VOG0083, VOG5722, VOG5779, VOG5721, VOG10940, VOG7134, VOG6941, VOG6036, VOG9219, VOG6472, VOG9713, VOG6940, VOG5534, VOG5601, VOG0537, VOG7930, VOG0275, VOG9667, VOG11003, VOG1070, VOG4779, VOG3389, VOG5042, VOG3498, VOG9712 |
| JAIEWK010000009.1 | 154237 | 167763 | False | Myoviridae / Siphoviridae | VOG4724, VOG1198, VOG1198, VOG0918, VOG4551, VOG0025, VOG7476, VOG0322, VOG6211 |
Phigaro v2.3.0 (Database: Aug. 2017 release)