Probiotic potential risk score
| ARGs (CARD) | VFs (VFDB) | PGs (PHI-base) | PPRS |
|---|---|---|---|
| 4 | 0 | 2 | 4.47 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| ARO accession | ARO name | Identity | Coverage | CARD name | Accession no. |
|---|---|---|---|---|---|
| ARO:3000179 | tet(L) | 99.13 | 100.00 | tet(L) | M11036.1 |
| ARO:3002837 | lnuC | 98.17 | 100.00 | lnuC | AY928180.1 |
| ARO:3000186 | tet(M) | 98.13 | 100.31 | tet(M) | AB039845.1 |
| ARO:3002844 | vatE | 97.20 | 100.00 | vatE | AF242872.1 |
RGI v6.0.0 (Database: CARD Variants v4.0.0, Jul. 2022 release)
ResFinder
| Resistance gene | Identity | Coverage | Phenotype | Accession no. |
|---|---|---|---|---|
| tet(L) | 99.78 | 100.00 | Tetracycline resistance | M29725 |
| lnu(C) | 99.19 | 100.00 | Lincosamide resistance | AY928180 |
| tet(M) | 99.12 | 100.00 | Tetracycline resistance | X75073 |
| vat(E) | 96.43 | 100.00 | Streptogramin B resistance | AJ488494 |
ResFinder v4.0 (Database: resfinder_db, May 2022 release)
AMRFinderPlus
| Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|
| vat(E) | streptogramin A O-acetyltransferase Vat(E) | BLASTX | 99.53 | 100.00 | WP_063856929.1 |
| tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 99.34 | 100.00 | WP_001574277.1 |
| tet(M) | tetracycline resistance ribosomal protection protein Tet(M) | BLASTX | 98.91 | 100.00 | WP_000691736.1 |
| lnu(C) | lincosamide nucleotidyltransferase Lnu(C) | BLASTX | 98.17 | 100.00 | WP_063851341.1 |
AMRFinderPlus v3.10.42 (Database: Oct. 2022 release)
Virulence factors
Virulence Factor Database (VFDB)
| Gene ID | Gene name | Identity | Coverage | e-value | VF name | VF category | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
BLASTN v2.8.1+ (Database: VFDB, Jun. 2022 release)
VirulenceFinder
| Database | Virulence factor | Identity | Coverage | Protein function | Accession no. |
|---|---|---|---|---|---|
| No hit found | |||||
VirulenceFinder v2.0.3 (Database: virulencefinder_db, Dec. 2022 release)
Pathogenic genes
PHI-base
| Protein ID | Gene name | Identity | Coverage | e-value | Function | Gene ID |
|---|---|---|---|---|---|---|
| S4E4Q5 | WalR | 85.59 | 100.00 | 1.01e-147 | Transcriptional regulatory protein | EPH95667 |
| Q82ZX2 | CspR | 81.82 | 98.51 | 2.20e-36 | Cold shock protein | AAO82613 |
BLASTP v2.8.1+ (Database: PHI-base v4.14, Nov. 2022 release)
Plasmid
PlasmidFinder
| Plasmid | Identity | Query / Template | Accession no. |
|---|---|---|---|
| No hit found | |||
PlasmidFinder 2.0.1 (Database: plasmidfinder_db, Jan. 2023 release)
Prophage
Phigaro
| Scaffold | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| JAINRO010000001.1 | 78972 | 112902 | False | Myoviridae | VOG4552, VOG6128, VOG0321, VOG4548, VOG4548, VOG0322, VOG6545, VOG4632, VOG4609, VOG0327, VOG8575, VOG5545, VOG0637, VOG5878, VOG0198, VOG0796, VOG10227, VOG0720, VOG4564, VOG9789, VOG4555, VOG4711, VOG3435, VOG1353, VOG1881, VOG1883, VOG1352, VOG4699, VOG1956, VOG1433, VOG1348, VOG0573, VOG4550, VOG4691, VOG4845, VOG4334, VOG4705 |
Phigaro v2.3.0 (Database: Aug. 2017 release)