Probiotic potential risk score

ARGs (CARD)	VFs (VFDB)	PGs (PHI-base)	PPRS
0	1	15	15.03

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

ARO accession	ARO name	Identity	Coverage	CARD name	Accession no.
No hit found

RGI v6.0.0 (Database: CARD Variants v4.0.0, Jul. 2022 release)

ResFinder

Resistance gene	Identity	Coverage	Phenotype	Accession no.
No hit found

ResFinder v4.0 (Database: resfinder_db, May 2022 release)

AMRFinderPlus

Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v3.10.42 (Database: Oct. 2022 release)

Virulence factors

Virulence Factor Database (VFDB)

Gene ID	Gene name	Identity	Coverage	e-value	VF name	VF category	Accession no.
VFG005323	cbpD	90.77	99.29	0.0	Autolysin	Exoenzyme	WP_011225268
VFG005555	tig/ropA	94.27	65.19	0.0	Trigger factor	Stress survival	WP_011225339
VFG006053	STU_RS14645	91.77	68.03	0.0	Capsule	Immune modulation	WP_011226048
VFG005381	srtA	85.60	64.44	1.90e-147	Sortase A	Exoenzyme	-

BLASTN v2.8.1+ (Database: VFDB, Jun. 2022 release)

VirulenceFinder

Database	Virulence factor	Identity	Coverage	Protein function	Accession no.
No hit found

VirulenceFinder v2.0.3 (Database: virulencefinder_db, Dec. 2022 release)

Pathogenic genes

PHI-base

Protein ID	Gene name	Identity	Coverage	e-value	Function	Gene ID
P67331	spr0084	93.19	98.18	0.0	pathogenitcity related	AAK98888
Q99Z66	ptsI	89.58	86.33	0.0	Phosphoenolpyruvate-protein phosphotransferase	AAK34199
P0C0B9	bglB	87.88	99.33	0.0	Putative phospho--glucosidase	AAK33487
D5AF41	IMPDH	87.83	99.80	0.0	Inosine-5'-monophosphate dehydrogenase	ADE32434
P0A452	recA	85.19	98.46	0.0	DNA recombination and repair	AAL00560
Q97RW0	SP_0676	83.11	93.50	0.0	Transcription facot	AAK74821
A4VY17	pdh	83.09	99.41	0.0	Pyruvate dehydrogenase	ABP91006
Q8DPC2	potA	91.97	70.98	1.04e-177	ABC transporter ATP-binding protein - spermidine/putrescine transport	AAL00050
Q1J943	prsA2	93.50	75.69	2.79e-164	Ribose-phosphate pyrophosphokinase	ABF36968
Q93MY4	covR	87.91	77.12	6.55e-118	Responder protein	AAK71319
U2W9V3	SpxA2	91.67	99.25	6.62e-89	Transcriptional regulator	ERL21461
Q8DQV4	spr0479	85.42	97.96	2.50e-58	essential gene	AAK99283
Q8CZ89	spr0175	85.23	98.88	1.52e-52	pathogenitcity related	AAK98979
Q8DRE1	spr0177	83.15	87.25	5.84e-51	essential gene	AAK98981
Q04M32	ilvC (SPD_0406)	97.35	99.71	0.0	Ketol-acid reductoisomerase (NADP(+))	ABJ55451
Q1JLQ2	MntE	94.81	64.17	3.65e-166	Manganese Homeostasis	ABF32167

BLASTP v2.8.1+ (Database: PHI-base v4.14, Nov. 2022 release)

Plasmid

PlasmidFinder

Plasmid	Identity	Query / Template	Accession no.
No hit found

PlasmidFinder 2.0.1 (Database: plasmidfinder_db, Jan. 2023 release)

Prophage

Phigaro

Scaffold	Begin	End	Transposable	Taxonomy	pVOGs
No hit found

Phigaro v2.3.0 (Database: Aug. 2017 release)