Probiotic potential risk score

ARGs (CARD)	VFs (VFDB)	PGs (PHI-base)	PPRS
0	2	8	8.25

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

ARO accession	ARO name	Identity	Coverage	CARD name	Accession no.
No hit found

RGI v6.0.0 (Database: CARD Variants v4.0.0, Jul. 2022 release)

ResFinder

Resistance gene	Identity	Coverage	Phenotype	Accession no.
No hit found

ResFinder v4.0 (Database: resfinder_db, May 2022 release)

AMRFinderPlus

Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v3.10.42 (Database: Oct. 2022 release)

Virulence factors

Virulence Factor Database (VFDB)

Gene ID	Gene name	Identity	Coverage	e-value	VF name	VF category	Accession no.
VFG005323	cbpD	91.55	100.00	0.0	Autolysin	Exoenzyme	WP_011225268
VFG006046	STR_RS05880	90.91	100.00	0.0	Capsule	Immune modulation	WP_011226149
VFG005381	srtA	84.79	64.44	5.90e-141	Sortase A	Exoenzyme	-

BLASTN v2.8.1+ (Database: VFDB, Jun. 2022 release)

VirulenceFinder

Database	Virulence factor	Identity	Coverage	Protein function	Accession no.
No hit found

VirulenceFinder v2.0.3 (Database: virulencefinder_db, Dec. 2022 release)

Pathogenic genes

PHI-base

Protein ID	Gene name	Identity	Coverage	e-value	Function	Gene ID
P67331	spr0084	92.88	98.18	0.0	pathogenitcity related	AAK98888
P0A452	recA	85.45	98.46	0.0	DNA recombination and repair	AAL00560
A4VYE0	gidA	85.42	98.75	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	ABP91129
Q1JLQ2	MntE	84.96	93.89	0.0	Manganese Homeostasis	ABF32167
U2W9V3	SpxA2	91.67	99.25	4.39e-89	Transcriptional regulator	ERL21461
Q8DQV4	spr0479	85.42	97.96	1.65e-58	essential gene	AAK99283
Q8CZ89	spr0175	85.23	98.88	1.01e-52	pathogenitcity related	AAK98979
Q8DRE1	spr0177	83.15	87.25	3.87e-51	essential gene	AAK98981
P0C0B9	bglB	88.94	66.56	4.00e-134	Putative phospho--glucosidase	AAK33487

BLASTP v2.8.1+ (Database: PHI-base v4.14, Nov. 2022 release)

Plasmid

PlasmidFinder

Plasmid	Identity	Query / Template	Accession no.
No hit found

PlasmidFinder 2.0.1 (Database: plasmidfinder_db, Jan. 2023 release)

Prophage

Phigaro

Scaffold	Begin	End	Transposable	Taxonomy	pVOGs
No hit found

Phigaro v2.3.0 (Database: Aug. 2017 release)