Probiotic potential risk score
| ARGs (CARD) | VFs (VFDB) | PGs (PHI-base) | PPRS |
|---|---|---|---|
| 1 | 11 | 19 | 21.98 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| ARO accession | ARO name | Identity | Coverage | CARD name | Accession no. |
|---|---|---|---|---|---|
| ARO:3000375 | ErmB | 97.54 | 82.26 | ErmB | AF242872.1 |
RGI v6.0.0 (Database: CARD Variants v4.0.0, Jul. 2022 release)
ResFinder
| Resistance gene | Identity | Coverage | Phenotype | Accession no. |
|---|---|---|---|---|
| mef(A) | 100.00 | 100.00 | Macrolide resistance | U83667 |
| msr(D) | 99.80 | 99.93 | Macrolide, Lincosamide and Streptogramin B resistance | AF274302 |
| tet(M) | 99.69 | 99.90 | Tetracycline resistance | X04388 |
| erm(B) | 99.60 | 100.00 | Macrolide resistance | AF299292 |
ResFinder v4.0 (Database: resfinder_db, May 2022 release)
AMRFinderPlus
| Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|
| mef(A) | macrolide efflux MFS transporter Mef(A) | EXACTX | 100.00 | 100.00 | WP_000417519.1 |
| erm(B) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) | PARTIALX | 99.09 | 89.39 | WP_001038792.1 |
| tet(M) | tetracycline resistance ribosomal protection protein Tet(M) | PARTIALX | 99.74 | 61.35 | WP_002414694.1 |
AMRFinderPlus v3.10.42 (Database: Oct. 2022 release)
Virulence factors
Virulence Factor Database (VFDB)
| Gene ID | Gene name | Identity | Coverage | e-value | VF name | VF category | Accession no. |
|---|---|---|---|---|---|---|---|
| VFG005556 | tig/ropA | 95.01 | 93.69 | 0.0 | Trigger factor | Stress survival | WP_011680653 |
| VFG006081 | rfbD | 94.95 | 100.00 | 0.0 | Capsule | Immune modulation | WP_014621820 |
| VFG006053 | STU_RS14645 | 94.11 | 81.01 | 0.0 | Capsule | Immune modulation | WP_011226048 |
| VFG006022 | rfbB | 93.89 | 99.90 | 0.0 | Capsule | Immune modulation | WP_002947383 |
| VFG005976 | STER_RS05305 | 93.52 | 100.00 | 0.0 | Capsule | Immune modulation | WP_011681174 |
| VFG006046 | STR_RS05880 | 92.81 | 74.75 | 0.0 | Capsule | Immune modulation | WP_011226149 |
| VFG006088 | STU_RS16255 | 91.22 | 99.65 | 0.0 | Capsule | Immune modulation | WP_041828290 |
| VFG005579 | eno | 90.58 | 99.92 | 0.0 | Streptococcal enolase | Exoenzyme | WP_000022815 |
| VFG006047 | STU_RS14640 | 89.06 | 99.71 | 0.0 | Capsule | Immune modulation | WP_011226047 |
| VFG005381 | srtA | 83.83 | 82.68 | 4.11e-172 | Sortase A | Exoenzyme | - |
| VFG006004 | SPD_RS01775 | 83.42 | 96.20 | 5.55e-155 | Capsule | Immune modulation | WP_001819836 |
| VFG019077 | plr/gapA | 93.07 | 67.06 | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | WP_000260685 |
| VFG006052 | rfbA | 90.96 | 60.92 | 0.0 | Capsule | Immune modulation | WP_011226150 |
BLASTN v2.8.1+ (Database: VFDB, Jun. 2022 release)
VirulenceFinder
| Database | Virulence factor | Identity | Coverage | Protein function | Accession no. |
|---|---|---|---|---|---|
| No hit found | |||||
VirulenceFinder v2.0.3 (Database: virulencefinder_db, Dec. 2022 release)
Pathogenic genes
PHI-base
| Protein ID | Gene name | Identity | Coverage | e-value | Function | Gene ID |
|---|---|---|---|---|---|---|
| Q54970 | spxB | 98.06 | 78.38 | 0.0 | Pyruvate Oxidase Gene | AAB40976 |
| P67331 | spr0084 | 92.88 | 98.18 | 0.0 | pathogenitcity related | AAK98888 |
| Q8DPC2 | potA | 90.30 | 96.11 | 0.0 | ABC transporter ATP-binding protein - spermidine/putrescine transport | AAL00050 |
| Q99Z66 | ptsI | 89.67 | 90.48 | 0.0 | Phosphoenolpyruvate-protein phosphotransferase | AAK34199 |
| A4VYE0 | gidA | 87.06 | 76.21 | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | ABP91129 |
| A4VWH9 | Eno | 86.41 | 99.31 | 0.0 | Enolase | ABP90468 |
| B5TQV9 | PGM | 85.56 | 78.36 | 0.0 | phosphoglucomutase | ACH87041 |
| A4VY17 | pdh | 83.09 | 99.41 | 0.0 | Pyruvate dehydrogenase | ABP91006 |
| A4VWD1 | mnmE | 82.61 | 80.13 | 0.0 | Central tRNA-modifying GTPase | ABP90420 |
| P95830 | DnaJ | 81.65 | 82.85 | 0.0 | Heat shock protein. PI3K and JNK signalling pathway | AAL14123 |
| Q1J943 | prsA2 | 93.09 | 75.69 | 1.33e-163 | Ribose-phosphate pyrophosphokinase | ABF36968 |
| P0C0B9 | bglB | 87.74 | 70.90 | 7.41e-141 | Putative phospho--glucosidase | AAK33487 |
| B3VA60 | CovR | 81.71 | 75.98 | 1.36e-103 | serves to repress GAS virulence factor-encoding genes | ACE75886 |
| U2W9V3 | SpxA2 | 91.67 | 99.25 | 1.50e-88 | Transcriptional regulator | ERL21461 |
| U2UML5 | SpxA1 | 80.00 | 85.19 | 5.59e-68 | Transcriptional regulator | ERL19273 |
| Q8CZ89 | spr0175 | 85.23 | 98.88 | 3.43e-52 | pathogenitcity related | AAK98979 |
| Q8DRE1 | spr0177 | 83.15 | 87.25 | 1.32e-50 | essential gene | AAK98981 |
| Q8DP67 | spr1327 | 93.15 | 84.88 | 2.84e-45 | essential gene | AAL00131 |
| Q04M32 | ilvC (SPD_0406) | 97.35 | 99.71 | 0.0 | Ketol-acid reductoisomerase (NADP(+)) | ABJ55451 |
| Q97S31 | metE | 93.16 | 68.27 | 0.0 | Methionine biosynthesis enzymes | AAK74738 |
| D5AF41 | IMPDH | 89.23 | 60.12 | 0.0 | Inosine-5'-monophosphate dehydrogenase | ADE32434 |
| Q1JLQ2 | MntE | 94.81 | 64.17 | 8.25e-166 | Manganese Homeostasis | ABF32167 |
| Q97RW0 | SP_0676 | 83.64 | 68.11 | 3.49e-135 | Transcription facot | AAK74821 |
| Q93MY4 | covR | 81.05 | 64.83 | 5.67e-88 | Responder protein | AAK71319 |
BLASTP v2.8.1+ (Database: PHI-base v4.14, Nov. 2022 release)
Plasmid
PlasmidFinder
| Plasmid | Identity | Query / Template | Accession no. |
|---|---|---|---|
| No hit found | |||
PlasmidFinder 2.0.1 (Database: plasmidfinder_db, Jan. 2023 release)
Prophage
Phigaro
| Scaffold | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.3.0 (Database: Aug. 2017 release)