Probiotic potential risk score

ARGs (CARD)	VFs (VFDB)	PGs (PHI-base)	PPRS
0	11	19	21.95

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

ARO accession	ARO name	Identity	Coverage	CARD name	Accession no.
No hit found

RGI v6.0.0 (Database: CARD Variants v4.0.0, Jul. 2022 release)

ResFinder

Resistance gene	Identity	Coverage	Phenotype	Accession no.
No hit found

ResFinder v4.0 (Database: resfinder_db, May 2022 release)

AMRFinderPlus

Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v3.10.42 (Database: Oct. 2022 release)

Virulence factors

Virulence Factor Database (VFDB)

Gene ID	Gene name	Identity	Coverage	e-value	VF name	VF category	Accession no.
VFG006081	rfbD	94.95	100.00	0.0	Capsule	Immune modulation	WP_014621820
VFG005556	tig/ropA	94.78	100.00	0.0	Trigger factor	Stress survival	WP_011680653
VFG006074	STU_RS16230	92.66	98.43	0.0	Capsule	Immune modulation	WP_011226325
VFG006084	STU_RS16245	92.48	99.88	0.0	Capsule	Immune modulation	WP_002948020
VFG006082	STU_RS16240	92.21	100.00	0.0	Capsule	Immune modulation	WP_011226326
VFG006086	STU_RS16250	91.95	100.00	0.0	Capsule	Immune modulation	WP_011226328
VFG005191	fbp54	91.39	99.94	0.0	FBPs	Adherence	WP_011681101
VFG006088	STU_RS16255	91.39	99.91	0.0	Capsule	Immune modulation	WP_041828290
VFG006078	STU_RS16235	90.91	99.15	0.0	Capsule	Immune modulation	WP_002948022
VFG005324	cbpD	90.07	93.50	0.0	Autolysin	Exoenzyme	WP_082302295
VFG005382	srtA	84.01	99.87	0.0	Sortase A	Exoenzyme	WP_011681303

BLASTN v2.8.1+ (Database: VFDB, Jun. 2022 release)

VirulenceFinder

Database	Virulence factor	Identity	Coverage	Protein function	Accession no.
No hit found

VirulenceFinder v2.0.3 (Database: virulencefinder_db, Dec. 2022 release)

Pathogenic genes

PHI-base

Protein ID	Gene name	Identity	Coverage	e-value	Function	Gene ID
P67331	spr0084	92.88	98.18	0.0	pathogenitcity related	AAK98888
Q1J943	prsA2	92.19	98.46	0.0	Ribose-phosphate pyrophosphokinase	ABF36968
Q8DPC2	potA	89.84	99.48	0.0	ABC transporter ATP-binding protein - spermidine/putrescine transport	AAL00050
Q8DYL6	uvrB	89.44	99.85	0.0	Excinuclease ABC, B subunit	AE014259_2
P0C0B9	bglB	87.88	99.33	0.0	Putative phospho--glucosidase	AAK33487
D5AF41	IMPDH	87.83	99.80	0.0	Inosine-5'-monophosphate dehydrogenase	ADE32434
A4VYE0	gidA	85.58	98.75	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	ABP91129
P0A452	recA	85.45	98.46	0.0	DNA recombination and repair	AAL00560
A4VY17	pdh	83.38	99.41	0.0	Pyruvate dehydrogenase	ABP91006
A4VWD1	mnmE	82.67	99.34	0.0	Central tRNA-modifying GTPase	ABP90420
Q93MY4	covR	85.53	99.58	2.76e-151	Responder protein	AAK71319
Q9S1K2	rr01	84.82	99.12	1.32e-145	Response regulator	CAB54566
R4Z8M6	ciaR	86.16	98.68	6.03e-144	Two component system response regulator	AIK75708
B3VA60	CovR	82.10	99.56	2.88e-138	serves to repress GAS virulence factor-encoding genes	ACE75886
U2UML5	SpxA1	82.09	99.26	4.72e-82	Transcriptional regulator	ERL19273
Q8DQV4	spr0479	85.42	97.96	1.21e-57	essential gene	AAK99283
Q8CZ89	spr0175	85.23	98.88	2.62e-52	pathogenitcity related	AAK98979
Q8DRE1	spr0177	83.15	87.25	1.00e-50	essential gene	AAK98981
Q04M32	ilvC (SPD_0406)	85.88	99.71	0.0	Ketol-acid reductoisomerase (NADP(+))	ABJ55451

BLASTP v2.8.1+ (Database: PHI-base v4.14, Nov. 2022 release)

Plasmid

PlasmidFinder

Plasmid	Identity	Query / Template	Accession no.
No hit found

PlasmidFinder 2.0.1 (Database: plasmidfinder_db, Jan. 2023 release)

Prophage

Phigaro

Scaffold	Begin	End	Transposable	Taxonomy	pVOGs
No hit found

Phigaro v2.3.0 (Database: Aug. 2017 release)